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  • Küçük Resim
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    Assessment of antimicrobial activity and In Vitro wound healing potential of ZnO nanoparticles synthesized with Capparis spinosa extract
    (Ekim 2023) Sezen, Selma; Ertuğrul, Muhammed Sait; Balpınar, Özge; Bayram, Cemil; Özkaraca, Mustafa; Okkay, Irmak Ferah; Hacımüftüoğlu, Ahmet; Güllüce, Medine
    Agents that will accelerate wound healing maintain their clinical importance in all aspects. The aim of this study is to deter mine the antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) ZnO nanoparticles obtained by green synthesis from Capparis spinosa L. extract and their efect on in vitro wound healing. ZnO NPs were synthesized and characterized using Capparis spinosa L. extract. ZnO NPs were tested against nine ATCC-coded pathogen strains to determine antimicrobial activity. The efects of diferent doses (0.0390625–20 µg/mL) of NPs on cell viability were determined by MTT assay. The efect of ZnO NPs doses (0.0390625 µg/mL, 0.078125 µg/mL, 0.15625 µg/mL, 0.3125 µg/mL, 0.625 µg/mL, 1.25 µg/mL) that increase proliferation and migration on wound healing was investigated in an in vitro wound experiment. Cell culture medium obtained from the in vitro wound assay was used for biochemical analysis, and plate alcohol-fxed cells were used for immunohistochemical staining. It was determined that NPs formed an inhibition zone against the tested Gram-positive bacteria. The ZnO NPs doses determined in the MTT test provided faster wound closure in in-vitro conditions compared to the DMSO group. Biochemical analyses showed that infammation and oxidative status decreased, while antioxidant levels increased in ZnO NPs groups. Immunohistochemical analyses showed increased expression levels of Bek/FGFR2, IGF, and TGF-β associated with wound healing. The fndings reveal the antimicrobial efect of ZnO nanoparticles obtained using Capparis spinosa L. extract in vitro and their potential applications in wound healing.
  • Küçük Resim Yok
    Öğe
    Neuroprotective effect of methanol extract of Capparis spinosa L. fruits in an in-vitro experimental model of Parkinson’s disease
    (MediHealth Academy Yayıncılık, 2022) Sezen, Selma; Yesilyurt, Fatma; Özkaraca, Mustafa; Bayram, Cemil; Alaylar, Burak; Güllüce, Medine; Hacımüftüoğlu, Ahmet
    Aim: Parkinson’s disease (PD) is the second most widespread neurodegenerative disease. This study, it was aimed to investigate the effect of methanol extract obtained from Capparis spinosa L. fruits, which are known to have important bioactive components, on in-vitro experimental PD model. Material and Method: After collecting Capparis spinosa L. fruits from Alanya/Antalya, methanol extract was prepared by drying and grinding. SH-SY5Y cells grown in flasks were transferred to 96 well plates and were incubated until 80% cell density was reached. Different doses of methanol extract were applied to the cells 30 minutes before the PD model was formed. For the PD model, SH-SY5Y cells were exposed to 200 µM 6-OHDA for 24 hours. MTT analysis was performed to assess the viability of SH-SY5Y cells at the end of the 24-hour period. TOS, TAC, and IL-17A levels in the cell medium were determined using the ELISA method. Expression of TNFα and α-synuclein was defined using the immunohistochemical method. Results: Cell viability was found to be higher in all treatment groups than in the 6-OHDA group. Moderate levels of TNFα and α-synuclein positivity were observed in the 1500 µg/ml methanol extract group. It was determined that TOS and TAC levels change depending on the dose. It has been determined that the level of IL-17A decreases at low doses. Statistical significance was found between the groups. Conclusion: When the findings were examined, it was determined that the methanol extract obtained from Capparis spinosa L. fruits reduced oxidative stress and IL-17A levels at low doses and provided a neuroprotective effect by increasing the antioxidant capacity.