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    CRYOPRESERVATION AND ASSESSMENT OF HONAMLI AND HAIR BUCK SEMEN USING RESVERATROL
    (Cryo Letters, 2025) Inanc, Muhammed Enes; Gungor, Sukru; Mart, Feyza Nur; Herdogan, Mine; Cay, Hasan Ali; Kahraman, Durmus; Korkmaz, Firat
    BACKGROUND: Resveratrol (Res) (3,5,4 '-trihydroxystilbene) is a natural polyphenol that exhibits important biological activities. OBJECTIVE: To assess the effects of resveratrol (Res) on freeze-thawed survival of semen from Honamli and Hair Bucks. MATERIALS AND METHODS: Six bucks, aged 2-3 years (three from each breed), were included in the study. Semen was collected from each breed and mixed separately after removing seminal plasma. The mixed semen was diluted with different Res concentrations (0 mu M as control, 25 mu M, 50 mu M, 100 mu M, 500 mu M, and 1 mM) in Tris diluent and subjected to cryopreservation in liquid nitrogen vapor and frozen. After thawing, the samples were evaluated for motility and some spermatologic quality parameters by flow cytometry. RESULTS: Data were analyzed separately for Honamli and Hair breeds. The results showed that the Res 1 mM group had the lowest motility in all assessments (P<0.05). However, no significant differences were observed between the other Res and control groups (P>0.05). In terms of apoptosis, Hair bucks exhibited a statistically significant difference in late apoptotic parameters, with the control showing the highest values (P<0.05). The Res 25 mu M group (similar to the control group) showed lower mitochondrial oxidative stress than the Res 1 mM group (P<0.05). CONCLUSION: Res at a dose of 1 mM did not protect most sperm functional and biochemical parameters except for apoptosis and performed worse than the control group. When all parameters are evaluated collectively, concentrations lower than 1 mM should be used for freezing Honamli and Hair Buck semen with resveratrol.
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    Impact of Trolox Supplementation on the Cryopreservation of Honamli Buck Semen
    (Mary Ann Liebert, Inc, 2025) Gungor, Sukru; Inanc, Muhammed Enes; Uslu, Baris Atalay; Burca, Ahmet Burak; Ata, Ayhan
    Cryopreservation of buck semen is essential in animal breeding but often damages sperm viability and integrity. The Honamli breed, a hardy Turkish goat, can benefit from improved freezing techniques using antioxidants such as Trolox (T). This study explores the effects of varying T concentrations on Honamli buck semen, assessing parameters such as motility, viability, and membrane integrity to enhance post-thaw quality. Findings support T's potential to improve semen extender formulations for preserving Honamli genetics.This study aims to freeze Honamli buck semen with T and to evaluate in vitro spermatological parameters. Three Honamli bucks, aged 2-3 years, were used in the study. Semen was collected from the bucks and mixed after removing seminal plasma. The mixed semen was diluted with a tris egg yolk extender containing three different concentrations of T (0.25 mM, 0.5 mM, and 1 mM) and control (0 mM). The diluted semen was equilibrated for 2 hours at +4 degrees and subjected to cryopreservation in liquid nitrogen vapor (-120 degrees C for 12 minutes) and frozen. After thawing (37 degrees C water bath for 30 seconds), the groups were evaluated at flow cytometric analysis for viability (SYBR/propidium iodide [PI]), plasma membrane acrosome integrity (FITC-PNA/PI), and mitochondrial membrane potential (JC-1), plasma membrane integrity (hypo-osmotic swelling test), microscopic evaluations for motility and morphological integrity (abnormal spermatozoa rate). The 0.5 T and 0.25 T groups showed significant improvements in motility compared with the control group (p < 0.05). The control group had the lowest plasma membrane integrity (p < 0.05). The highest morphological integrity was observed in the T groups compared with the control group (p < 0.05). In conclusion, supplementing T in buck semen extenders benefits spermatological parameters; particularly, 0.25 and 0.5 mM T could be used in Tris semen extenders during the cryopreservation process.