Yazar "Haligur, Mehmet" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Canine Parvoviral Enteritis- The Role of MMP-9 and TIMP-1 in the Pathogenesis of Intestinal Inflammation
    (Univ Fed Rio Grande Do Sul, 2024) Karayigit, Mehmet Onder; Aydogan, Ahmet; Haligur, Mehmet; Basbug, Onur; Karatas, Ozhan
    Background: Canine parvoviral enteritis is a highly contagious infection in the intestines caused great morbidity and mortality in untreated dogs younger than 6 months. Matrix metalloproteinases consist of zinc- and calcium-dependent extracellular matrix-degrading endopeptidases that are tightly controlled by endogenous metalloproteinase tissue inhibitors. Canine parvoviral enteritis is common in Turkey. The aim of this study was to examine the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in natural canine parvoviral enteritis infection of 25 dogs diagnosed with parvoviral enteritis by clinical tests and histopathology. Materials, Methods & Results: The study material consists of dog's small intestine, which was brought to Cumhuriyet University Faculty of Veterinary Medicine pathology department for necropsy and diagnosed with parvoviral enteritis. This investigation was suported by the Comission of Scientific Research Projects of Cumhuriyet University (Project No: V-086). For the study, sections of 5 mu m were taken from small intestine blocks consisting of duodenum, jejunum and ileum, fixed in 10% buffered formalin solution and embedded in paraffin, and stained with hematoxylin-eosin and MMP-9 and TIMP-1 antibodies using immunohistochemical procedure. On histopathology, shedding and blunting of the villi epithelium, severe mononuclear inflammation in the lamina propria and locally enlarged crypts with lymphocytolysis in peyer's patches were noted in the ileum. Immunohistochemically, strong expression for MMP-9 and moderate expression for TIMP-1 were observed in the crypt epithelium and inflammatory cells in the small intestines of infected animals compared controls (P < 0.001). Discussion: In the present study, immunohistochemical expressions of MMP-9 and TIMP-1 in intestinal tissues were investigated in canine parvoviral enteritis, which is an important viral disease in veterinary medicine. Statistically strong expression for MMP-9 and moderate expression for TIMP-1 were observed in the crypt epithelium and inflammatory cells in the small intestines of infected animals. As a result, high levels of MMP-9 may be one of the factors that trigger the inflammatory process in the disease. It is thought that the increase in MMP-9 may be directly proportional to the severity of inflammation in the tissue. In addition, it is suggested that the level of its inhibitor, TIMP-1, may increase at similar rates in response to this increase in MMP-9 levels. As a result, severe increases MMP-9 and TIMP-1 may indicate the presence of inflammation of similar severity in that tissue. Immunohistochemical data obtained from the study showed that MMP-9 expression was found to increase in inflammatory and degenerative changes in parvoviral enteritis. This may have triggered extracellular matrix degradation, intestinal permeability, degenerative changes and inflammation. Abnormal increase in MMP-9 levels is thought to contribute significantly to the intestinal lesions in parvoviral enteritis. It was observed that TIMP-1 levels increased similarly in response to this increase but weaker expression of TIMP-1 as its inhibitor in canine parvoviral enteritis may determine the development of the disease. In this regard, matrix metalloproteinases appear to be potential therapeutic targets in canine parvoviral enteritis, and the use of their inhibitors can significantly reduce disease progression. However, current findings need to be confirmed by more detailed studies in the future.
  • Küçük Resim Yok
    Öğe
    Immunohistochemical expression of MMP-2 and MMP-9 in the brain tissue of sheep naturally infected with Listeria monocytogenes and relationship with cell death in the Listerial encephalitis
    (Univ Zulia, Facultad Ciencias Veterinarias, 2024) Karayigit, Mehmet Onder; Haligur, Mehmet; Ekici, Mehmet
    Listeria monocytogenes is an intracellular, food-borne bacterium. Silage is an important source of this pathogen causing listeriosis. Listeriosis is an important health problem for both animals and humans in the world. The disease comprises three clinical syndromes: meningoencephalitis, septicemia and metritis with abortion. Encephalitis is frequently observed and the factors that play a role in its pathogenesis are the subject of research. In this study, the immunohistochemical expression of MMP-2 and MMP-9 together with TUNEL staining was investigated in the pathogenesis of meningoencephalitis in sheep naturally infected with L. monocytogenes. The brains of 25 sheep with Listerial meningoencephalitis were used in this study. Brain material from 10 sheep provided from the slaughterhouse was also used as a control. Tissue sections were stained immunohistochemically with L. monocytogenes, MMP-2 and MMP-9 antibodies. Additionally, TUNEL staining was performed to determine apoptosis in the disease. As a result of the study, it was observed that TUNEL staining in neurons and glial cells, MMP-2 and MMP-9 expressions in vascular endothelial cells, inflammatory cells, microglia and especially neurons in the infected brain tissue were significantly increased compared to controls. These results suggested that MMP-2 and MMP-9 play an active role in the neurodegeneration and cell death that occur in Listerial encephalitis.