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    Can SCUBE1 be used to predict the early diagnosis, lesion volume and prognosis of acute ischemic stroke?
    (WALTER DE GRUYTER GMBH, 2019) Bolayir, Asli; Gokce, Seyda Figul; Cigdem, Burhanettin; Bolayir, Hasan Ata; Gulunay, Aydin; Celik, Veysel Kenan; Kapancik, Serkan; Yildiz, Oztem Kayim
    Objective: SCUBE1 [signal peptide-CUB (complement C1r/C1 s)-EGF (epidermal growth factor)-like domain-containing protein 1] is a novel biochemical marker. SCUBE1 is thought to play roles both in platelet activation and inflammation, which are important stages for the development of acute ischemic stroke (AIS). The purpose of our study was to determine the diagnostic and prognostic values and temporal change of plasma SCUBE1 levels in AIS patients. Materials and methods: Thirty-five patients diagnosed with AIS at the Cumhuriyet University Faculty of Medicine Neurology Department, between June and December 2017, and a control group of 35 healthy volunteers were included. Results: Median first day SCUBE1 value in the patient group was 97.51 ng/mL, and the median 7th day SCUBE1 value was 32.72 ng/mL. Median control group SCUBE1 value was 27.51 ng/mL. The first day SCUBE1 levels were significantly higher than the 7th day and the control group SCUBE1 levels (p = 0.001, p < 0.001, respectively). The ROC analysis showed that SCUBE1 levels above 68.8 ng/mL can be used as an indicator with high sensitivity and specificity for AIS diagnosis. Multivariate analysis revealed that the first day SCUBE1 had significant independent effects on development of AIS. In correlation analysis, plasma SCUBE1 levels showed a significantly positive correlation with lesion volume, NIHSS and MRS values (p < 0.01). Conclusion: First day plasma SCUBE1 values in AIS patients rised at significant levels compared to the control group. SCUBE1 could use both in the early diagnosis and prediction of prognosis and lesion volume of AIS patients.
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    Chemical composition, cytotoxicity, and molecular docking analyses of Thuja orientalis extracts
    (Elsevier, 2024) Kapancik, Serkan; Celik, Muhammed Safa; Demiralp, Mustafa; Unal, Kagan; Cetinkaya, Serap; Tuzun, Burak
    There has been an increasing interest in the biologically active plant extracts. Studies still continue in order to discover novel components especially with antimicrobial activities to overcome the challenge of drug resistance and to ameliorate existing antibiotics. Three extract preparations, methanol, water, and chloroform, were made. Their chemical compositions were determined and compared. Extraction with water proved most fruitful as it contained forty-two active compounds. Cytotoxicity of these compounds was tested on MCF-7 and HT-29 cell lines and it was demonstrated that ethanol extracts were toxic. Molecular docking computations hinted that some of the compounds could target breast cancer proteins PDB ID, 1A52, and 1JNX and colorectal cancer proteins PDB ID, 1UWH, and 3RQ7). ADME/T calculations were made to examine the effects of Thuja orientalis extracts on human metabolism.
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    New benzimidazole derivatives containing hydrazone group as anticancer agents: Inhibition of carbonic anhydrase IX and molecular docking studies
    (Wiley-V C H Verlag Gmbh, 2025) Bostanci, Hayrani Eren; Yildiz, Mehmet Taha; Kapancik, Serkan; Inan, Zeynep Deniz Sahin; Kilic, Haci Ahmet; Guler, Ozen Ozensoy; Cevik, Ulviye Acar
    In this study, we propose identifying potential novel compounds targeting carbonic anhydrase (CA) IX and anticancer activity. To study the impact of these synthesized compounds on CA IX and anticancer activity, we have developed and synthesized novel benzimidazole-hydrazone derivatives (3a-3j). The target compounds' H-1 NMR (nuclear magnetic resonance), C-13 NMR, and high resolution mass spectrometry spectra were used to confirm their chemical structures. L929 (healthy mouse fibroblast cell line) used as control healthy cell line and MCF-7 (breast cancer), C6 (rat glioblastoma), HT-29 (colon cancer), cells were used in cell culture studies. As a result of cell culture studies, it was determined that the newly synthesized compounds 3d and 3j had cytotoxic effects on colon cancer. Again, it was determined that the compound 3d had a more toxic effect than cisplatin on both breast cancer and glioma cells. According to the CA IX activity results, compounds 3d and 3j were found to have the highest activity. Compounds 3d and 3j are essential for having anti-cancer properties and inhibiting the carbonic anhydrase IX enzyme. Molecular docking of these compounds was carried out in the active site of CA IX. Flow cytometry and immunofluorescence microscope analyses also confirmed that these compounds had cytotoxic effects on cancer cells.
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    Serum levels of polyamine synthesis enzymes increase in diabetic patients with breast cancer
    (BIOSCIENTIFICA LTD, 2017) Celik, V. Kenan; Kapancik, Sercan; Kacan, Turgut; Kacan, Selen Baloglu; Kapancik, Serkan; Kilicgun, Hasan
    Background: In this study, it was aimed to investigate the relationship between diabetes and breast cancer and the detection of enzymes and ornithine levels in polyamine synthesis pathway in diabetes, breast cancer and diabetic breast cancer patients. Methods: Ornithine, arginine decarboxylase, ornithine decarboxylase and agmatinase levels have been measured in serum of all groups. Ornithine levels were measured spectrophotometrically. Arginine decarboxylase, ornithine decarboxylase and agmatinase levels were determined by ELISA kits. Results: Except for the diabetic group, the levels of enzymes in the polyamine synthesis pathway were increased in all and statistically significant (P < 0.05). The increase in the levels of agmatinase was very important among the enzymes (P < 0.001). Conclusions: Decreased levels of polyamine synthase enzymes in diabetes mellitus were found to be increased patients with breast cancer. Whether and how diabetes-based breast cancer development relates to increase activity of enzymes responsible for polyamine synthesis requires further mechanistic and prospective monitoring studies in larger patient cohorts.
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    Synthesis and Characterization of Magnetic Stearic Acid Modified Sulfated Alginate and Investigation of the Cytotoxicity and Apoptosis in MCF-7 Cell Line
    (Springer, 2024) Akkaya, Birnur; Kapancik, Serkan; Akkaya, Recep; Sariaydin, Nurguel
    Breast cancer is the type of cancer that causes death in the first place among women in 2022, with a rate of 6.9%. According to the data of the World Health Organization in 2024, 665,684 women died due to breast cancer in 2022. Cancer metastasis is the main reason why people die from cancer. Since changes in proliferation-related gene expression and protein levels that occur as a result of mutations that cause metastasis make cancerous cells more proliferatively aggressive, treatment opportunities remain limited. Sulfatide has the potential to be a p-selectin inhibitor, as it is a p-selectin-binding molecule that has an antimetastatic effect. Sulfatide mimicking material has previously been studied by sulfonation and acylation of chitosan. It was evaluated as an anticancer drug carrier because it did not have cytotoxic effects against cancer cells. In this study, a sulfatide-like molecule was obtained using alginate and it was found to have a cytotoxic effect against cancer cells. The compound that is newly synthesized in the presented article is an alternative to the group of sulfatide-like compounds that we have previously studied, which are thought to have an antimetastatic effect. The molecular disruption of the apoptosis mechanism in cancerous tissues leads to the resistance of cancerous cells to apoptosis, which in turn promotes cancer progression. For this reason, cancer studies focus on cell death, that is, apoptosis, as well as on cell proliferation. This study is the first to combine the simultaneous sulfation and acylation of alginate with the addition of magnetism, despite previous research on the sulfation, acylation, and alkylation of alginate (M-SA-SULF-A) and analysed it by scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy, a vibrating sample magnetometer (VSM) and a mastersizer. Then we aim to examine the effect of newly modified magnetic stearic acid on apoptosis in MCF-7 cells by looking at the expressions of apoptosis-and metastatasis related genes through an array kit, and to reveal the molecular pathways that cause modified alginate's anti-cancer activity. Thus, we determined that magnetic stearic acid modified sulfated alginate may be an anti-cancer agent by regulating the expression of genes associated with apoptosis.
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    The relationship of agmatine deficiency with the lung cancer
    (UHOD - Uluslararasi Hematoloji Onkoloji Dergisi, 2016) Kapancik, Serkan; Celik, V. Kenan; Kilickap, Saadettin; Kacan, Turgut; Kapancik, Sercan
    The polyamines are essential for rapidly growing cells. Polyamines is synthesized by two reactions which begin from arginine. The aim of this study is to examine the serum levels of the enzymes located in the putrescine synthesis pathway and their relationship with lung cancer. In addition, the study also aim determine the oxidant-antioxidant status and the oxidative stress index. This study was performed on 36 lung cancer patients and 36 healthy controls. The arginase activity and the ornithine level were determined spectrophotometrically while the arginine decarboxylase, ornithine decarboxylase and agmatinase levels were determined by using Enzyme Linked Immunosorbent Assay (Elisa kit). The total antioxidant (TAS) and total oxidant status (TOS) were determined using a commercial kit auto analyser, oxidative stress index (OSI) was subsequently estimated. We observed an increase in ornithine decarboxylase, agmatinase and arginase levels in cancer patients (p< 0.05). however, no significant difference was observed between the two groups with regards to orithine and arginase decarboxylase levels (p> 0.05). In the patient group, the TAS values decreased (p< 0.05), while the TOS values increased (p< 0.05). In addition, the OSI values increased (p< 0.05). Reduced agmatine level can cause an increase in polyamine synthesis and cell proliferation. For this reason, lung cancer prognosis may have a relationship with the agmantine levels. © 2016, UHOD - Uluslararasi Hematoloji Onkoloji Dergisi. All rights reserved.