Yazar "Karatas, Ozhan" seçeneğine göre listele
Listeleniyor 1 - 14 / 14
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Aflatoxin B-1 induced renal and cardiac damage in rats: Protective effect of lycopene(ELSEVIER SCI LTD, 2018) Yilmaz, Seval; Kaya, Emre; Karaca, Aysegul; Karatas, OzhanThis study was conducted to investigate the protective effects of lycopene against the toxic effects of Aflatoxin B-1 (AFB(1)) exposure in kidney and heart of rat by evaluating antioxidant defense systems and lipid peroxidation (LPO). Forty-two healthy three-month-old male Wistar-Albino rats were used in this study. The animals were randomly divided into six experimental groups including 7 rats in each. These groups were arranged as follows: control group, lycopene (5 mg/kg/day, orally for 15 days) group, AFB(1) (0.5 mg/kg/day, orally for 7 days) group, AFB(1) (1.5 mg/kg/day, orally for 3 days) group, AFB(1)(0.5 mg/kg/day, orally for 7 days) + lycopene (5 mg/kg/ day, orally for 15 days) group and AFB(1) (1.5 mg/kg/day, orally for 3 days) + lycopene (5 mg/kg/day, orally for 15 days) group. The animals were sacrificed at the end of applications. In this study, malondialdehyde (MDA) levels significantly increased; while reduced glutathione (GSH), glutathione-S-transferase (GST), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and glucose-6-phosphate-dehydrogenase (G6PD) activities decreased in kidney and heart tissues. The significant reduction in the activities of antioxidant enzymes and non-enzymatic antioxidant system in AF treated rats as compared to the control group could be responsible for increased MDA levels observed during AF induced kidney and heart damage. The results showed increased urea, creatinine levels, as well as reduction sodium concentrations in plasma of AFB(1 )treated rats. There was lycopene showed protection against AF induced nephrotoxicity and cardiotoxicity.Öğe An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia(Urmia Univ, 2024) Karatas, Ozhan; Akcakavak, GokhanContagious caprine pleuropneumonia (CCPP) in goats is defined as a highly contagious and rapidly spreading mycoplasmal disease that is now among the leading causes of major economic losses on many continents (Asia, Africa and the Middle East). In this study, we aimed to evaluate immunohistochemically mast cells (MCs) profile and local interleukin (IL)-17 and IL-1(3 protein expressions in naturally infected CCPP according to the course of the inflammation (peracute-acute, subacute-chronic). The material of the study consisted of 40 naturally infected CCPP and 6 healthy control goat lung tissues. Appropriate samples were taken from the necropsied goats and subjected to histopathological and immunohistochemical examination. In the histopathological examination of the samples, it was determined that 29 samples had a peracute-acute course and 11 had a subacute-chronic course. In immunohistochemical examination, MC profile and local IL-17 and IL-1(3 protein expressions were evaluated in the peracute-acute and subacute-chronic course. Immunohistochemically, significant increases in MC number, local IL-17 and IL-1(3 scores were detected in the peracuteacute course compared to the control group. There were significant decreases in the relevant scores in the subacute-chronic course compared to the peracute-acute course. Current findings indicated that MC, IL-17, and IL-1(3 expressions played important roles in the pathogenesis of infection in naturally infected CCPP, especially in the peracute-acute course. Additionally, MC profile was evaluated for the first time in naturally infected CCPP. (c) 2024 Urmia University. All rights reserved.Öğe Canine Parvoviral Enteritis- The Role of MMP-9 and TIMP-1 in the Pathogenesis of Intestinal Inflammation(Univ Fed Rio Grande Do Sul, 2024) Karayigit, Mehmet Onder; Aydogan, Ahmet; Haligur, Mehmet; Basbug, Onur; Karatas, OzhanBackground: Canine parvoviral enteritis is a highly contagious infection in the intestines caused great morbidity and mortality in untreated dogs younger than 6 months. Matrix metalloproteinases consist of zinc- and calcium-dependent extracellular matrix-degrading endopeptidases that are tightly controlled by endogenous metalloproteinase tissue inhibitors. Canine parvoviral enteritis is common in Turkey. The aim of this study was to examine the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in natural canine parvoviral enteritis infection of 25 dogs diagnosed with parvoviral enteritis by clinical tests and histopathology. Materials, Methods & Results: The study material consists of dog's small intestine, which was brought to Cumhuriyet University Faculty of Veterinary Medicine pathology department for necropsy and diagnosed with parvoviral enteritis. This investigation was suported by the Comission of Scientific Research Projects of Cumhuriyet University (Project No: V-086). For the study, sections of 5 mu m were taken from small intestine blocks consisting of duodenum, jejunum and ileum, fixed in 10% buffered formalin solution and embedded in paraffin, and stained with hematoxylin-eosin and MMP-9 and TIMP-1 antibodies using immunohistochemical procedure. On histopathology, shedding and blunting of the villi epithelium, severe mononuclear inflammation in the lamina propria and locally enlarged crypts with lymphocytolysis in peyer's patches were noted in the ileum. Immunohistochemically, strong expression for MMP-9 and moderate expression for TIMP-1 were observed in the crypt epithelium and inflammatory cells in the small intestines of infected animals compared controls (P < 0.001). Discussion: In the present study, immunohistochemical expressions of MMP-9 and TIMP-1 in intestinal tissues were investigated in canine parvoviral enteritis, which is an important viral disease in veterinary medicine. Statistically strong expression for MMP-9 and moderate expression for TIMP-1 were observed in the crypt epithelium and inflammatory cells in the small intestines of infected animals. As a result, high levels of MMP-9 may be one of the factors that trigger the inflammatory process in the disease. It is thought that the increase in MMP-9 may be directly proportional to the severity of inflammation in the tissue. In addition, it is suggested that the level of its inhibitor, TIMP-1, may increase at similar rates in response to this increase in MMP-9 levels. As a result, severe increases MMP-9 and TIMP-1 may indicate the presence of inflammation of similar severity in that tissue. Immunohistochemical data obtained from the study showed that MMP-9 expression was found to increase in inflammatory and degenerative changes in parvoviral enteritis. This may have triggered extracellular matrix degradation, intestinal permeability, degenerative changes and inflammation. Abnormal increase in MMP-9 levels is thought to contribute significantly to the intestinal lesions in parvoviral enteritis. It was observed that TIMP-1 levels increased similarly in response to this increase but weaker expression of TIMP-1 as its inhibitor in canine parvoviral enteritis may determine the development of the disease. In this regard, matrix metalloproteinases appear to be potential therapeutic targets in canine parvoviral enteritis, and the use of their inhibitors can significantly reduce disease progression. However, current findings need to be confirmed by more detailed studies in the future.Öğe Determination by Immunohistochemistry of Acute Phase Proteins in Naturally Infected Sheep with Listeriosis(Sciendo, 2025) Karatas, Ozhan; Akcakavak, GokhanListeriosis is an infectious and fatal disease affecting domestic mammals, poultry, and humans worldwide. The effectiveness of local tissue expression of acute phase proteins in listeriosis in domestic mammals is not yet clear. The aim of this study is to evaluate the local expression of acute phase proteins in 26 brainstem tissue samples according to the distribution and severity of inflammation due to natural Listeriosis disease in sheep. The study material consisted of 26 brainstem paraffin blocks, including 20 from listeriosis-infected cases and 6 from healthy controls. Sections obtained from the paraffin blocks were subjected to histopathological and immunohistochemical analyses. Histopathological examination revealed normal histological structures in the brainstems of the control group. In contrast, brainstem sections from listeriosis cases exhibited histopathological findings such as micro abscesses composed of neutrophil granulocytes and microglial cells, gliosis, meningitis, congestion, perivascular cuffs, neuronal degeneration, and neuronophagia. Based on the distribution and severity of inflammation, listeriosis cases were categorized into three groups: 5 mild cases (Group I), 8 moderate cases (Group II), and 7 severe cases (Group III). Immunohistochemical analysis demonstrated significantly increased expression levels of C-reactive protein (CRP), Haptoglobin (Hp), and Serum Amyloid A (SAA) in listeriosis groups compared to the control group, with the highest statistical scores observed in Group II and Group III (p<0.001). The findings of this study suggest that acute-phase proteins may play crucial roles in the pathophysiological processes of naturally infected listeriosis and could express locally. Particularly, the increased expression of these proteins with the progression of inflammation may provide valuable insights into disease severity and the infection process.Öğe Determination of Apoptosis, Necroptosis and Autophagy Markers by Real-time PCR in Naturally Infected Pneumonic Pasteurellosis caused by Pasteurella multocida and Mannheimia haemolytica in Cattle(Univ Agriculture, Fac Veterinary Science, 2024) Akcakavak, Gokhan; Karatas, Ozhan; Tuzcu, Nevin; Tuzcu, MehmetPneumonic pasteurellosis (PP) is defined as one of the pivotal infectious diseases caused by Pasteurella multocida and Mannheimia haemolytica . This study aimed to determine the levels of Bcl-2-associated protein X (Bax), B-cell lymphoma 2 (Bcl2), caspase-3, autophagy related-5 (Atg5), beclin-1 and receptor interacting protein3 ( RIP3) in lung tissues with naturally infected PP caused by P. multocida and M. haemolytica , and to reveal their effects on the pathogenesis of P. multocida and M. haemolytica pneumonia. The material of the study consisted of 150 fibrinous pneumonia/pleuropneumonia and 10 healthy lung tissue samples. Relevant samples were examined by histopathological, immunohistochemical and real-time PCR methods. Immunohistochemically, 23 (15.3%) were positive for P. multocida, and 17 (11.3%) were positive for M. haemolytica . Subsequently, the processes of apoptosis, autophagy and necroptosis for P. multocida and M. haemolytica were evaluated by real-time PCR. P. multocida pneumonia increased Bax, Caspase-3, Atg5, Beclin-1, and RIP3 gene expressions (4.2, 3.8, 2.9, 2.1, 2.8-fold, respectively), whereas Bcl-2 gene expression was decreased (0.22-fold). While Bax, Caspase-3, Atg5, Beclin-1, and RIP3 gene expressions were increased in M. haemolytica pneumonia (2.3, 1.9, 1.7, 1.2, 4.2-fold, respectively), it was observed that Bcl-2 gene expression was reduced (0.52-fold). The results obtained in the study revealed the importance of necroptosis, apoptosis and autophagy processes in the pathogenesis of PP caused by P. multocida and M. haemolytica and contributed to the literature. In addition, we found that the processes of apoptosis and autophagy play a more active role in PP caused by P. multocida , and the process of necroptosis plays a more active role in PP caused by M. haemolytica.Öğe Eucalyptol regulates Nrf2 and NF-kB signaling and alleviates gentamicin-induced kidney injury in rats by downregulating oxidative stress, oxidative DNA damage, inflammation, and apoptosis(Taylor & Francis Ltd, 2024) Akcakavak, Gokhan; Kazak, Filiz; Karatas, Ozhan; Alakus, Halil; Alakus, Ibrahim; Kirgiz, Omer; Celik, ZeynepGentamicin, an aminoglycoside antibiotic, is nowadays widely used in the treatment of gram-negative microorganisms. The antimicrobial, anti-inflammatory, and antioxidant activities of eucalyptol, a type of saturated monoterpene, have been reported in many studies. The aim of this study was to examine the possible effects of eucalyptol on gentamicin-induced renal toxicity. A total of 32 rats were divided into 4 groups; Control (C), Eucalyptol (EUC), Gentamicin (GEN), and Gentamicin + Eucalyptol (GEN + EUC). In order to induce renal toxicity, 100 mg/kg gentamicin was administered intraperitoneally (i.p.) for 10 consecutive days in the GEN and GEN + EUC groups. EUC and GEN + EUC groups were given 100 mg/kg orally of eucalyptol for 10 consecutive days. Afterwards, rats were euthanized and samples were taken and subjected to histopathological, biochemical, immunohistochemical, and real-time PCR examinations. The blood urea nitrogen (BUN) and creatinine (CRE) levels were significantly decreased in the GEN + EUC group (0.76 and 0.69-fold, respectively) compared to the GEN group. The glutathione peroxidase (GPx) and catalase (CAT) activities were significantly increased in the GEN + EUC group (1.35 and 2.67-fold, respectively) compared to the GEN group. In GEN group, Nuclear factor kappa B (NF-kB), Interleukin 1-beta (IL-1 beta), Inducible nitric oxide synthase (iNOS), Tumor necrosis factor-alpha (TNF-alpha), Caspase-3, 8-Hydroxy-2 '-deoxyguanosine (8-OHdG) and Nuclear factor erythroid 2-related factor (Nrf2) expression levels were found to be quite irregular. GEN + EUC group decreased the expressions of NF-kB, IL-1 beta, iNOS, TNF-alpha, Caspase-3, and 8-OHdG (0.55, 0.67, 0.54, 0.54, 0.63 and 0.67 fold, respectively), while it caused increased expression of Nrf2 (3.1 fold). In addition, eucalyptol treatment ameliorated the histopathological changes that occurred with gentamicin. The results of our study show that eucalyptol has anti-inflammatory, antioxidative, antiapoptotic, nephroprotective, and curative effects on gentamicin-induced nephrotoxicity.Öğe Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2024) Karatas, Ozhan; Akcakavak, GokhanWhite muscle disease (WMD) and/or nutritional muscular dystrophy is defined as a disease with a generally acute course that results in degeneration and necrosis of the heart muscle. In this study, it was aimed to reveal local tissue expressions of acute phase proteins such as C–reactive protein (CRP), Serum amyloid–A (SAA) and Haptoglobin (Hp) in lambs with WMD. The study material consisted of 27, one–six months old lamb heart tissues, 6 of which were healthy and 21 with WMD. The lambs were necropsied, and the relevant heart samples were taken into neutral formaldehyde. Afterwards, paraffin blocks were obtained by going through routine tissue follow–up processes. Sections were taken from paraffin blocks and stained with Hematoxylin–Eosin (H–E) and Immunohistochemical methods. Histopathologically, control groups exhibited normal histology. Hyaline degeneration, Zenker necrosis, calcification, inflammatory cell infiltration and an increase in connective tissue were detected in the heart tissues of lambs with WMD. Lambs with WMD had significantly increased CRP and SAA proteins compared to control (P<0.01). However, there was no difference between the groups in Hp (P>0.05). In conclusion, local tissue expressions of CRP, SAA and Hp in lambs with WMD were identified immunohistochemically for the first time. It is possible to say that CRP and SAA may play important roles in the pathophysiology of WMD and that CRP and SAA may provide more sensitive results in the diagnosis and prognosis of the disease. © (2023), (Universidad del Zulia). All Rights Reserved.Öğe Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected(Wiley, 2025) Karatas, Ozhan; Ozkaraca, Mustafa; Atasoy, Mustafa O.This study aimed to detect the presence of bovine papillomavirus (BPV) in the testicular tissue of bulls over 1-year old by immunohistochemical, immunofluorescence and molecular assay targeting methods. In addition, gamma H2AX and cytochrome c expressions were evaluated by immunohistochemical and immunofluorescent methods in samples positive for BPV agent. In this study, 100 testicular specimens that did not show any macroscopic papilloma findings were collected. Testicular tissues were collected and examined using histologically and molecularly methods. In the present study, immunopositivity was detected in 6 (6%) samples with the IHC method and in 11 (11%) samples with the IF method. In addition, BPV positivity was detected in four (4%) samples by the PCR method and four (4%) samples determined to be BPV positive by PCR also showed immunopositivity in IHC and IF methods. gamma H2AX immunopositivity was seen in two of the BPV-positive samples by the IHC method, and gamma H2AX immunopositivity was observed in four of the samples determined to be positive for the BPV agent by the IF method. Cytochrome c immunopositivity was found in all the samples determined positive for the BPV agent. When the findings were evaluated, the presence of the BPV agent in the testicular tissues of cattle that did not have macroscopic papillomas was revealed for the first time. It was thought that the BPV agent caused double-stranded DNA breaks and increased cytochrome c expression.Öğe Mechanism of anticancer effect of gambogic acid on gastric signet ring cell carcinoma(Humana Press Inc, 2023) Joha, Ziad; Ozturk, Aysegul; Yulak, Fatih; Karatas, Ozhan; Ataseven, HilmiGambogic acid has demonstrated inhibitory effects on the growth of various cancer cell types, such as breast cancer, pancreatic cancer, prostate cancer, lung cancer, and osteosarcoma. This study aims to investigate the antiproliferative activity of Gambogic acid on SNU-16 cells derived from gastric signet ring cell carcinoma and elucidate the underlying mechanisms. The cytotoxic effect of gambogic acid was evaluated in SNU-16 cells by treating them with different concentrations of the compound, and the XTT cell viability assay was employed to assess cell viability. ELISA was used to measure bax, BCL-2, caspase 3, PARP, and 8-oxo-dG levels. Additionally, immunofluorescence staining was applied to assess 8-oxo-dG and LC3 & beta; levels in SNU-16 cells. It was observed that gambogic acid exerted a dose-dependent and statistically significant antiproliferative effect on SNU-16 cells. The IC50 value of gambogic acid in SNU-16 cells was found to be 655.1 nM for 24 h. Subsequent investigations conducted using the IC50 dose revealed a significant upregulation of apoptotic proteins including cleaved caspase 3, Bax, and cleaved PARP (p < 0.001), along with a downregulation of BCL-2 (p < 0.001), an anti-apoptotic protein. Moreover, the administration of this drug led to an upregulation of 8-oxo-dG (p < 0.001), a widely acknowledged biomarker indicating oxidative damage in DNA, as well as an increase in LC3 & beta; levels (p < 0.05), a marker associated with autophagy. The antiproliferative effect of gambogic acid against gastric signet ring cell carcinoma is attributed to its ability to induce apoptosis and autophagy. This discovery highlights the promising potential of gambogic acid as a treatment option for gastric signet ring cell carcinoma.Öğe Proanthocyanidin alleviates testicular torsion/detorsion-induced ischemia/ reperfusion injury in rats(Churchill Livingstone, 2024) Kazak, Filiz; Akcakavak, Gokhan; Alakus, Ibrahim; Alakus, Halil; Kirgiz, Omer; Karatas, Ozhan; Deveci, Mehmet Zeki YilmazTesticular torsion is an urological emergency and can lead to ischemia damage and testicular loss if not diagnosed in time. Proanthocyanidin is reported to have anti-inflammatory and antioxidant properties. The current study aimed to examine the possible effects of proanthocyanidin (P) on the testis in torsion/detorsion (T/D)induced testicular ischemia/reperfusion (I/R) injury in rats. Forty rats were divided into four groups (n=10 for each): sham-operated (sham), I/R, I/R + P100 (100 mg/kg, 30 min before torsion), and I/R + P200 (200 mg/kg, 30 min before torsion). Testicular T/D was performed on the left testicle by 3 hours of torsion at 720 degrees clockwise, followed by 3 hours of detorsion. In the I/R group, an increase in malondialdehyde (MDA) levels and a decrease in glutathione (GSH), vitamin C (Vit C), glutathione peroxidase (GPx), glucose-6-phosphate dehydrogenase (G6PD) values were determined compared to the sham group (p <0.001). Moreover, an increase in the expression of cleaved caspase-3 and Bcl2-associated X protein (Bax), a decrease in the expression of B-cell lymphoma 2 (Bcl2) and proliferating cell nuclear antigen (PCNA) were detected in the I/R group (p <0.001). Histopathologically, it was determined that the Johnsen and Cosentino scores of the testicles were irregular in the I/R group (p <0.001). Proanthocyanidin treatment caused a decrease in MDA, cleaved caspase-3 and Bax levels and an increase in GSH, Vit C, GPx, G6PD, Bcl-2 and PCNA values. Additionally, Johnsen and Cosentino rearranged the scores. The present findings revealed the protective and curative effects of proanthocyanidin in organ damage due to testicular torsion/detorsion-induced ischemia/reperfusion with their antioxidative and antiapoptotic properties.Öğe Syringic acid guards against indomethacin-induced gastric ulcer by alleviating inflammation, oxidative stress and apoptosis(Taylor & Francis Ltd, 2024) Okkay, Irmak Ferah; Okkay, Ufuk; Cicek, Betul; Karatas, Ozhan; Yilmaz, Aysegul; Yesilyurt, Fatma; Hacimuftuoglu, AhmetThe purpose of this study was to evaluate the effects of syringic acid, an anti-oxidant, on indomethacin induced gastric ulcers in rats. Experimental groups were control, ulcer, ulcer treated with 20 mg/kg esomeprazole (a proton pump inhibitor that reduces acid secretion), and ulcer treated with 100 mg/kg syringic acid. Rats were pretreated with esomeprazole or syringic acid two weeks before ulcer induction. Our histopathological observations showed that either syringic acid or esomeprazole attenuated the severity of gastric mucosal damage. Moreover, syringic acid and esomeprazole pretreatments alleviated indomethacin-induced damage by regulating oxidative stress, inflammatory response, the level of transforming growth factor-beta (TGF-beta), expressions of COX and prostaglandin E2, cell proliferation, apoptosis and regulation of the NF-kappa B signaling pathway. We conclude that either esomeprazole or syringic acid administration protected the gastric mucosa from harmful effects of indomethacin. Syringic acid might, therefore be a potential therapeutic agent for preventing and treating indomethacin-induced gastric damage.Öğe Tarantula cubensis alcohol extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats(University of Benin, 2024) Akcakavak, Gokhan; Celik, Zeynep; Karatas, Ozhan; Dogan, Osman; Ozdemir, Ozgur; Tuzcu, MehmetPurpose: To evaluate the effect of a combination of Tarantula cubensis alcohol extract (TCAE) and capecitabine (CAP) in the treatment of azoxymethane (AOM)-induced colorectal cancer (CRC). Methods: Forty-two Wistar albino rats were divided into 7 groups with 6 rats in each group. The groups consisted of Control (C), Control+TCAE (C-TCAE), Control+CAP (C-CAP), Cancer control (CC), Cancer+TCAE (CC-TCAE), Cancer+CAP (CC-CAP) and Cancer+CAP+TCAE (CC-CAP+TCAE). To induce CRC, AOM (15 mg/kg) was administered to rats subcutaneously (sc) twice at a one-week interval to all the groups except control. From the 15th week, TCAE (0.2 mL/rat sc) was administered to CC-TCAE group every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered by gavage to CC-CAP group for 4 weeks. In CC-CAP+TCAE group, TCAE (0.2 mL/rat sc) was administered every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered gavage for 4 weeks. Animals were treated for 18 weeks. Aberrant crypt foci (ACF) were evaluated histopathologically among CC, CC-TCAE, CC-CAP, and CC-CAP+TCAE groups. ?-catenin, CD15, Proliferating Cell Nuclear Antigen (PCNA), and Nuclear Factor kappa B (NF-?B) expression levels were immunohistochemically compared among all groups. Results: Histopathologically, ACF scores were significantly increased in CC group, while a significant decrease in the relevant scores (p < 0.001) was observed in CC-CAP and CC-CAP+TCAE treatment groups, and the lowest scores were in CC-CAP+TCAE group. Immunohistochemically, in CC group, ?-catenin, Nuclear Factor kappa B (NF-?B), Proliferating Cell Nuclear Antigen (PCNA) and CD15 expressions were highly irregular. CC-CAP and CC-CAP+TCAE groups had significantly reduced expressions (p < 0.001), and the lowest expressions were in CC-CAP+TCAE group. Conclusion: The combined use of TCAE and CAP in treatment of CRC has a synergistic effect and increases the anticancer efficacy of TCAE, and CAP. More studies at the molecular level are needed in the future to demonstrate the clinical benefit of TCAE supplementation during the treatment of CRC with CAP. © 2024 The authors. This work is licensed under the Creative Commons Attribution 4.0 International License.Öğe The effects of sorafenib in healthy and cisplatin-treated rats(Wroclaw University of Medicine, 2023) Demirtas, Levent; Gürbüzel, Mehmet; Tahirler, Hilal; Akbas, Emin Murat; Karatas, Ozhan; Arslan, Yusuf KemalBackground. Sorafenib is a multikinase inhibitor currently used in the treatment of hepatocellular carcinoma, renal cell carcinoma and thyroid cancer. Objectives. The literature on this agent is scarce. This study aimed to evaluate the effects of sorafenib when administered to both healthy and cisplatin-induced rats. Materials and methods. The animals were divided into 4 groups: 1) control group that received 0.9% saline intraperitoneally (C); 2) group administered a single dose (7 mg/kg) of cisplatin (Cis); 3) a group administered 20 mg/kg of sorafenib for 7 days (Sor); 4) group administered 20 mg/kg of sorafenib followed by 7 mg/kg of cisplatin for 7 days (Cis+Sor). All animals were sacrificed 7 days after the completion of their treatment arm, and serum and tissue samples were taken. Results. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and interleukin 38 (IL-38) levels were increased in the Sor and Cis+Sor groups compared to the control group. When compared with the control group, serum urea, creatinine, kidney IL-1β, and tumor necrosis factor alpha (TNF-α) levels did not change in the Sor group. When compared to the Cis group, the levels of these parameters decreased in the Cis+Sor group. Conclusions. According to the data obtained, sorafenib caused liver toxicity when given to both healthy and cisplatin-induced rats. While sorafenib did not cause any significant changes in the kidneys when given to healthy rats, it had a healing effect in kidneys after stress induced by cisplatin. © 2023 Wroclaw University of Medicine. All rights reserved.Öğe The Effects of Sunitinib in Healthy and Cisplatin-Induced Rats(Wiley-V C H Verlag Gmbh, 2023) Demirtas, Levent; Gurbuzel, Mehmet; Akbas, Emin Murat; Tahirler, Hilal; Karatas, Ozhan; Arslan, Yusuf KemalSunitinib is a multitargeted kinase inhibitor that inhibits many receptor tyrosine kinases and has been used in the treatment of gastrointestinal stromal tumors, metastatic renal cell carcinoma, and pancreatic neuroendocrine tumors. In this study, the effects of sunitinib given to rats, both alone and after stress with cisplatin, were investigated. The animals were divided into four groups - (1) control group (C) administered interperitoneally with a single dose 0.9 % saline, (2) Cis group administered a single dose (7 mg/kg) of cisplatin, (3) Sun group administered 10 mg/kg sunitinib for seven days, and (4) Cis+Sun group administered 10 mg/kg sunitinib for seven days after a single dose (7 mg/kg) of cisplatin. After these applications, the rats were sacrificed, and blood and tissue samples were taken for biochemical and histopathological evaluations. Sunitinib did not show any effect on urea, creatine, and kidney IL1 beta and TGF-beta 3 expression levels when administered alone; it increased ALT, AST, and IL-38 levels. When sunitinib was given to the cisplatin-induced rats, it was observed that the increase in ALT, AST, and IL-38 levels increased more than the rats that was given only sunitinib. According to the data obtained, sunitinib does not cause a significant change in kidney tissue under both normal and stress conditions, while it creates stress in liver tissue. In addition, its toxicity in the liver becomes more certain as a result of its combination with cisplatin.
