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Öğe An Insight Into Veterinary Students' Perceptions on the use of 3D-Printed Bone Biomodels in Anatomy Learning(Univ Ljubljana, 2023) Kocyigit, Alper; Ari, Hasan Huseyin; Uslu, Baris AtalayToday, conventional teaching methods are losing their effectiveness at transferring knowledge and skills, prompting the presentation of alternative strategies that hold more promise. One of the innovative alternative education materials in veterinary anatomy education is the models produced on three-dimensional (3D) printers. The subject of this study is 4 different bone biomodels 3D modeled and printed with reference to cadaver-derived bones. In the study, a total of 298 students were asked to evaluate these biomodels in terms of their similarity to the reference bones. According to the survey, 75.5% of the students stated that their biomodel resembled the reference bones. In addition, 64.8% of these students stated that the use of biomodels can be efficient in learning the skeletal system. These outcomes showed that a sample from each of the 4 main bone types could be replicated on a 3D printer with an acceptable similarity ratio. Based on student opinions about these four different biomodels, we think that 3d printed biomodels deserve to be evaluated as an alternative in anatomy education.Öğe Correlation between the cryosurvival, cell number and diameter in bovine in vitro produced embryos(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2016) Kocyigit, Alper; Cevik, MesutThe selection of quality embryos is a prerequisite of cryopreservation process. Present study was conducted to examine the correlation between the diameter and cryotolerance, on the cell number of the cryopreserved embryos. The blastocyst stage embryos were collected at culture days 7-9, evaluated morphologically under a microscope and divided according to the diameter into three groups: Group 1; (larger than 150 mu m), Group 2; (diameter of 100-150 mu m), Group 3; (smaller than 100 mu m). Blastocysts were vitrified-thawed using the classical vitrification method and then cultured in SOF medium drops at 24 h. Blastocysts were considered viable if they re-expanded or hatched from the zona pellucidae. Finally re-expanded blastocysts from the Group 1 and Group 2 to determine the differential count of cells in the ICM and TE. The re-expansion ability of blastocysts 100-150 mu m in diameter (69.56%) was significantly higher than other groups (52.17 and 47.36%). The value of the correlation coefficient between the re expansion rate and cell number of blastocysts in the group 2 (r = 0.784) tended to be higher than that in the group 1 (r = 0.512) and group 3 (r = 0.491) (p < 0.05). For ICM/total cell ratio yield group 2 embryos showed higher rate (0.28), compared to the other groups (0.19 and 0.16). In conclusion, the present study demonstrates that the correlation between diameter of embryos and their cryosurvival based on re-expansion ability and cell allocation. (C) 2016 Elsevier Inc. All rights reserved.Öğe Effect of SpermVital® technology on conception rate in repeat breeder multiparous dairy cows: preliminary results(Tubitak Scientific & Technological Research Council Turkey, 2019) Kocyigit, Alper; Uslu, Baris Atalay; Sendag, Sait; Treupel, Elisabeth; Wehrend, AxelThe aim of this study was to investigate the effect of SV technology on conception rates in repeat breeder multiparous dairy cows. Seventy-nine multiparous Holstein cows from a private dairy farm were used in the study. These animals were cows that had failed to conceive from at least 3 regularly spaced services (repeat breeders). Estrus cycles of the cows were synchronized by 2 injections of the PG analogue, administered 11 days apart. GnRH was applied 48 h after the second injection of PG. Twenty-four h after this administration, the animals were randomly divided into 2 groups, control and SV. The animals in the control group (n = 28) were inseminated with standard processed semen, and the cows in the SV group (n = 51) were inseminated with SV (R) technology processed semen. A lower pregnancy rate (35.5%) was determined in the control group than in the SV (47.1%) group. The difference between pregnancy rates in the groups was statistically significant (P < 0.05). We are at too early a stage to say that SV (R) Technology can fully respond to the deficiencies in herd management. This work may also lead to future studies into the use of more animal material.Öğe Effects of leukemia inhibitory factor and insulin-like growth factor-I on the cell allocation and cryotolerance of bovine blastocysts(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015) Kocyigit, Alper; Cevik, MesutThe present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and LIP on bovine embryo development potential and (2) to investigate the cryotolerance and survivability of vitrified blastocysts obtained from embryos cultured in a defined media. We studied the development of bovine embryos produced in vitro and cultured (in four different treatments) until Day 7 after fertilization. In Experiment 1, zygotes were cultured to the blastocyst stage and differentially stained for determine the count of cells. In Experiment 2, zygotes were vitrified before staining. LIF alone or combined with IGF-I was significantly effective on in vitro bovine embryo development especially ratio to reach blastocyst. The cells for both ICM and TE decreased by the effect of freezing in all treatment groups in the Experiment 2 compared with Experiment I. Interestingly, the LIF treatment showed fewest variations. In addition to this, for average number of ICM and TE cells, LIP treatment showed fewest variation compared with other treatments (ICM: 23.5 vs 19.5, TE: 53.6 vs 51). These results are the first to demonstrate that the addition of IGF-I along with LIP to the culture medium was found to be beneficial for bovine embryonic development based on cellular cryotolerance after vitrification. (C) 2015 Elsevier Inc. All rights reserved.Öğe Kangal Akkaraman koçlarında bazı androlojik özelliklerin belirlenmesi(2021) Kocyigit, Alper; Yılmaz, Oğuz BurakBu çalışmada Kangal Akkaraman koçlarının androlojik özelliklerinin incelenmesi, bu özelliklerin mevsimle ilişkisinin araştırılması amaçlandı. Bu amaçla dokuz adet Kangal Akkaraman koçunda testis ölçüleri belirlendi. Hayvanlardan elektro-ejakulatör yöntemiyle sperma alındı. Ayrıca kan alınarak serum testosteron ve çinko seviyeleri araştırıldı. Koçlarda testislere ait ölçüm sonuçlarına göre testis uzunluğu mevsim dışı grupta ortalama 11,72 ± 1,30 cm iken mevsim içi grupta 12,21 ± 1,30 cm olarak belirlendi. Spermatolojik muayeneler sonucu sperma miktarı mevsim dışı grupta ortalama 1,02 ± 0,21 ml iken mevsim içi grupta 1,26 ± 0,12 ml olarak ölçüldü. Koçlarda biyokimyasal analiz sonuçlarına göre serum testosteron düzeyi mevsim dışı grupta ortalama 1,77 ± 0,53 ng/ml iken mevsim içi grupta 6,46 ± 1,33 ng/ml olarak belirlendi. Çinko düzeyi ise mevsim dışı grupta ortalama 87,58 ± 11,22 ?g/dL iken mevsim içi grupta 83,97 ± 13,46 ?g/dL olarak ölçüldü. Çalışma sonucunda Akkaraman ırkının, Kangal varyetesinin iri vücut yapısı testis morfolojisine de yansıdığı görüldü. Koçların sperm konsantrasyonu dışındaki spermatolojik özellikleri ile serum testosteron seviyesi Akkaraman ırkı ile paraleldir. Bu çalışma sonuçlarının, suni tohumlama uygulamasını da içeren ve gebelik oranı bulgularıyla desteklenecek başka çalışmalarla geliştirilmesi gerektiğini düşünmekteyiz.Öğe The effect of GnRH on the pregnancy ratio in low-yielding local race cows: comparison of different injection times(Springer, 2020) Uslu, Baris Atalay; Kocyigit, Alper; Sendag, Sait; Gulyuz, Fetih; Wehrend, AxelThe objective of this article was to investigate the efficiency of GnRH administrations at different time points after induced luteolysis on pregnancy rates in low-yielding subfertile cows. One thousand six hundred and ten healthy and subfertile dairy cows of different ages and races were used in this study. Cows were randomly divided into 4 groups. Estrus cycles were synchronized by two, with 11-day intervals, injections of the prostaglandin F2 alpha-analogue (PG). The artificial inseminations (AIs) of all animals were achieved at the 72nd and 96th hours following the last PG injection. The animals in groups I (n 257), II (n 337), and III (n 675) were used for the administration of a single dose of GnRH at different time points. Accordingly, GnRH was applied at 48th, 64th, and 72nd hours following the last PG injection in groups I, II, and III, respectively. Group IV was accepted as a control without GnRH injection (n 341). The pregnancy rates in groups I, II, III, and IV after transrectal pregnancy examinations were found to be 89.88%, 91.09%, 83.25%, and 77.12%, respectively. In our study, maximal pregnancy rates could be obtained with GnRH injections performed at 48th and 64th hours following luteolysis induction (P < 0.001). There was a 6-8% decrease in pregnancy rates due to the injection of GnRH in the 72nd hour (P < 0.001). These dramatic losses and gains in pregnancy rates in our study emphasized the necessity of taking the time of injection into account when using GnRH to stimulate ovulation. It can be said that the success of GnRH stimulation of ovulation is directly related to the follicle wave dynamics at the time of injection point and the character of a dominant follicle.Öğe The production of testis biomodels using three-dimensional (3D) technologies(Wiley, 2021) Kocyigit, Alper; Narlicay, SalihThis study aimed to investigate the potentiality of biomodels to be produced as alternative tools to slaughterhouse materials in andrology education. For this purpose, testis biomodels were produced with reference to bull testes. The biomodel production was carried out by the following steps: the preparation of the reference organs, 3D modelling, and processing of data sets and stages. The biomodels and reference testes were compared in terms of morphological parameters and tonicity. As a result of quantitative measurements, the average length in the reference testicles was 145.56 +/- 21.3 mm, while the thickness was 61.94 +/- 17.2 mm. The average length, thickness, volume and tonicity values of the biomodels showed similarity to the values of the reference testicles (p > .05). However, it was recorded that the average weight of the reference testicles was determined as 368.07 +/- 40.3 g, while the average weight of the biomodels was 69.02 +/- 3.18 g (p < .01). As a result, it has been shown that testis biomodels can be successfully produced using three-dimensional technologies. These biomodels are the first examples in the field. We think that the biomodels produced by using innovative technologies should be considered as serious alternatives, which could contribute to the learning processes of students, especially in andrology education.
