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Öğe Amniotic Membrane-Coated Polypropylene Mesh for the Repair of Incisional Hernia: An Experimental Study In a Rat Model of Abdominal Wall Defect(KOWSAR PUBL, 2018) Soylu, Sinan; Yildiz, Caglar; Bozkurt, Birkan; Karakus, Savas; Kurt, Begum; Kurt, AtillaBackground: Incisional hernia repairs are among common abdominal wall surgeries, can be primarily required or being reconstructed using a synthetic or biological material. Objectives: This study aimed at evaluating intra-abdominal adhesions and incisional site healing after the repair of abdominal wall by fresh amniotic membrane-coated polypropylene mesh in comparison to only polypropylene mesh in an experimental rat study. Methods: The study protocol was approved by Cumhuriyet University Institutional Ethics Committee for Animal Experiments (Sivas-Turkiye, date 24/06/2015). Sixteen pregnant female Wistar-Albino rats (mean weight, 275 g) were anesthetized on the 21st day of pregnancy and a 1-cm area of the abdominal wall was excised. The pregnancy was terminated, emerging amniotic membranes were dissected, and eight pieces of the 1-cm(2) polypropylene mesh were coated with these amniotic membranes without using any suture or adhesive. The polypropylene meshes were sutured on the abdominal wall of eight rats (control group), selected by simple random sampling. For the remaining eight rats, the same procedure was applied with the amniotic membrane-coated polypropylene meshes (experimental group). On the 28th postoperative day, the anterior abdominal wall was opened, and intra-abdominal adhesions were assessed macroscopically by Nair's adhesion scoring system. Strip-shaped biopsy samples were taken from incision lines for histopathological examination. Results: The experimental group had significantly less intra-abdominal adhesions (i.e. Nair's score of 2 to 4) compared to the control group (two and six rats, respectively; P= 0.046), and had significantly lower mean score for polymorphonuclear leukocyte infiltration (P = 0.039), hyperemia (P= 0.039), and epithelialization (P= 0.039). The score for increase in connective tissue (P = 0.018) was significantly higher in the experimental group, and the scores for edema (P= 0.590) and macrophage infiltration (P= 0.590) were similar between the two groups. Conclusions: The use of polypropylene mesh coated with fresh amniotic membrane provides advantage of decreasing postoperative intra-abdominal adhesions along with less inflammation and higher epithelialization after abdominal wall repair surgery.Öğe Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)(Sivas Cumhuriyet University, 2021) Genç, Hüsnü; Soylu, Sinan; İnan, Deniz Şahin; Kurt, Atilla; Çoşkun, Hakkı; Yıldırır, AliObjective: The basic aim in the treatment of second-degree burns is to prevent progressive cell death, and so treatments are directed at the zone of stasis. In this study, We were aimed to investigate the healing effects of using Mesenchymal Stem Cells (MSCs), Ovarian follicular fluid (OFS) and Platelet-rich plasma (PRP) on burn stasis zone in an experimental burn model.Method: Forty rats were divided randomly into four groups. Burns were created in each group according to the comb burn model. The control group received no treatment; the mesenchymal stem cellÖğe Effects of contrast media on the hepato-pancreato-biliary system(W J G PRESS, 2009) Topcu, Omer; Kurt, Atilla; Nadir, Isilay; Arici, Sema; Koyuncu, Ayhan; Aydin, CengizAIM: To determine the effects of high osmolarity contrast media (HOCM) and iso-osmolar contrast. media (CM) application, with or without pressure, on hepato-pancreato-biliary (HPB) system. METHODS: Sixty rats were divided into six equal groups as follows: Group 1: (0.9% NaCl, control), Group 2: (diatrizoate meglumine Na, ionic HOCM, Urographin (R)), Group 3: (iodixanol, iso-osmolar nonionic CM, Visipaque (R)); each of which was applied without pressure, whereas the animals of the remaining three groups (1p, 2p, 3p) were subjected to the same CM with pressure. We performed a duodenal puncture and introduced a catheter into the ampulla. After the catheterization, 0.2 mL CM or 0.9% NaCl was injected with or without pressure. Blood samples were taken for biochemical evaluations. The histopathological examinations of liver, common bile duct, and pancreas were performed. RESULTS: There were no significant differences between the six groups for blood amylase, alanine aminotransferases, aspartate aminotransferases, bilirubin levels (P > 0.05). Alkaline phosphatase and gamma glutamyl transaminase levels were higher (P < 0.05) in the Urographin (R) groups (2, 2p) than the Visipaque (R) groups (3, 3p), or control groups (1, 1p). Hepatocyte necrosis, portal area inflammation, and Kupffer's cell hyperplasia were higher (P < 0.05) in the study groups than the control group. However, there were no significant differences (P > 0.05) between HOCM (2, 2p) and iso-osmolar CM (3, 3p) groups. Bile duct proliferation and regeneration in the Urographin (R) groups (2, 2p) were significantly higher (P < 0.05) than the Visipaque (R) groups (3, 3p) or the control groups (1, 1p). Although CM caused minor damage to the pancreas, there were no statistically significant differences (P > 0.05) between the groups. Application of the CM with pressure did not cause additional damage to the HPB system. CONCLUSION: Iso-osmolar, non-ionic CM could be more reliable than the ionic HOCM, whereas the application of pressure during the CM application had no effect on the HPB system. (C) 2009 The WJG Press and Baishideng. All rights reserved.Öğe Effects of Intraperitoneal Bevacizumab Administration on Colonic Anastomosis and Early Postoperative Adhesion Formation(SAGE PUBLICATIONS INC, 2013) Karanlik, Hasan; Kurt, Atilla; Kunduz, Enver; Serin, Kursat; Saglam, Sezer; Soydinc, Hilal Oguz; Yasasever, Vildan; Olgac, Vakur; Asoglu, OktarAim. The purpose of this study is to investigate the effect of intraperitoneal (IP) bevacizumab on colonic anastomosis and evaluate the effects on early postoperative adhesion formation. Materials and Methods. A total of 24 mature female Sprague-Dawley rats were used for this study. Rats were randomly assigned to a control group that received saline (n = 8) or to experimental groups (n = 8 each) that received bevacizumab at a dose of 2.5 mg/kg (group 1) or 5 mg/kg (group 2). Animals were killed humanely on the seventh day after operation, and measurements of anastomotic strength and biochemical variables were performed. Results. The mean adhesion grade was 2.63 +/- 0.92, and 1 +/- 0.93 and 0.75 +/- 0.71 for the control and test groups, respectively. Bevacizumab significantly reduced adhesion formation in both low-dose and high-dose IP applications (P < .05). When all groups were compared, it was found that VEGF levels decreased significantly only in the tissue (P = .001), whereas there was no significant difference in the blood and the IP fluid (P = .73 and .08, respectively). We evaluated hydroxyproline levels, anastomosis bursting pressure, and histopathological healing scores. When each of these parameters were examined, there was statistical difference between groups (P = .01, .004, and .01, respectively). It was found that these parameters significantly decreased depending on increasing drug dose. Conclusion. IP administration of bevacizumab effectively reduced the formation of adhesions and caused significant impairment of anastomotic wound healing when standard doses were administered (5 mg/kg), but the 2.5-mg/kg dosage did not affect the anastomotic wound healing and also effectively reduced the formation of adhesions.Öğe Effects of Proton Pump Inhibitors and H-2 Receptor Antagonists on the Ileum Motility(HINDAWI PUBLISHING CORPORATION, 2011) Kurt, Atilla; Altun, Ahmet; Bagcivan, Ihsan; Koyuncu, Ayhan; Topcu, Omer; Aydin, Cengiz; Kaya, TijenObjectives. To investigate the effects of proton pump inhibitors (PPIs) and H-2 receptor antagonists on ileum motility in rats with peritonitis and compare changes with control group rats. Methods. Peritonitis was induced by cecal ligation and puncture in 8 rats. Another of 8 rats underwent a sham operation and were accepted as controls. Twenty-four hours later after the operation, the rats were killed, and their ileum smooth muscle was excised and placed in circular muscle direction in a 10mL organ bath. Changes in amplitude and frequency of contractions were analyzed before and after PPIs and H-2 receptor blockers. Results. PPI agents decreased the motility in a dose-dependent manner in ileum in both control and intraabdominal sepsis groups. While famotidine had no significant effect on ileum motility, ranitidine and nizatidine enhanced motility in ileum in both control and intraabdominal sepsis groups. This excitatory effect of H-2 receptor antagonists and inhibitor effects of PPIs were significantly high in control group when compared to the peritonitis group. The inhibitor effect of pantoprazole on ileum motility was significantly higher than the other two PPI agents. Conclusions. It was concluded that H-2 receptor antagonists may be more effective than PPIs for recovering the bowel motility in the intraabdominal sepsis situation.Öğe Effects of strontium ranilate and Hypericum perforatum extract on experimental colitis model in rats(2018) Kurt, Atilla; Soylu, Sinan; İnal, Zeynep Deniz Şahin; Topçu, Ömer; Atabey, MustafaObjective: In this study, we aimed to compare the healing effect of strontium ranilate(SR) and hypericum perfaratum(HPE) with the healing effect of prednisolone used in the treatment of experimental colit in rats.Method: 30 wistar albino rats were used in the study. Rats were randomly assigned to 5 consecutive groups consistingof 5 rats. Group 1, healthy rats, group 2 colitis but untreated rats. The rats in groups 3, 4, and 5 had colitis and received160 mg / kg SR, 1mg/kg prednisolone and HP extracts containing 0.3% hiperisine (250 uq / 300 mg) by oral gavage,respectively for seven days. At the end of the seventh day, the colons of the rats were removed and histopathologicalexamination was performed.Results: The histopathological results of the HPE group were statistically worst including the control group. It wasobserved that the best results in the treatment groups were SR group followed by prednisolone group,respectively.(P<0.05)Conclusions: This study concluded that SR was at least as effective as prednisolone in colitis treatment, but HPEexacerbate colitis-induced inflammation when received by orally.Öğe Guidelines for Extended Lymphadenectomy in Gastric Cancer: A Prospective Comparative Study(SPRINGER, 2013) Asoglu, Oktar; Matlim, Tugba; Kurt, Atilla; Onder, Semen Yesil; Kunduz, Enver; Karanlik, Hasan; Sam, Bulent; Kapran, Yersu; Bugra, DursunTo assess the efficacy of extended lymph node dissection in gastric cancer and to identify factors affecting lymph node detection. A prospective study of 126 gastric cancer patients was conducted. Patients eligible for curative resection received total gastrectomy and extended lymphadenectomy (D2) and paraaortic lymph node sampling as the standard of care (study group). Supramesocolic total lymphadenectomy of the upper gastrointestinal tract was performed on 23 autopsy cases as a control group. Fifty-five gastric carcinoma patients were included in the study group. Median age was 58 years (range 31-80 years); 14 patients were female (25 %), and 41 were male (75 %). The median number of lymph nodes harvested from the specimen was 47 (24-95), and the median number of metastatic lymph nodes was 15 (1-71). In contrast, in the autopsy comparative group, the median number of harvested lymph nodes was 72 (50-91). The median number of stational lymph nodes excised (lymph nodes excised from stations 4, 5, 10, 11, 12, and 16) was significantly higher in the control group than in the study group (P < 0.05). Lymph node detection was adversely affected by body mass index (BMI) (P < 0.03). In the study group, stations 5, 12, 11, and 10 had the highest lymph node absence (LNA) (noncompliance) ratio with percentages of 53, 36, 33, and 22 %, respectively. In the autopsy group, LNA (noncompliance) was not detected. Lymph nodes should be dissected by surgeons with sufficient technical and anatomical experience, and then examined and counted by experienced pathologists to reduce the occurrence of LNA. The results of this anatomical study can serve as a guideline to assess the success of lymph node dissection during gastric cancer surgery. Similar studies should be conducted in every country to establish national guidelines.Öğe The impact of Ramadan on peptic ulcer perforation(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2012) Gokakin, Ali Kagan; Kurt, Atilla; Atabey, Mustafa; Koyuncu, Ayhan; Topcu, Omer; Aydin, Cengiz; Sen, Metin; Akgol, GunduzBACKGROUND Medical treatment has played an important role in the reduction of peptic ulcer perforation (PUP). The goal of this study was to evaluate the effect of fasting on PUP. METHODS A retrospective analysis of 229 patients who were operated due to PUP between 1999-2009 was made. Patients were divided into two groups. Group I (n=188) included the patients who were operated in other periods of the year, while Group II (n=41) included the patients who were operated during Ramadan, the Muslim period of fasting. Patients in Group II were analyzed in terms of duration of fasting. RESULTS The increase in surgeries per group was higher in Group II than Group I (p<0.05). Predisposing factors, anti-ulcer drug usage and demographic variables were seen to have no role in this difference. Duration of fasting may have a minimal effect on the perforation. CONCLUSION The results of this study demonstrate that PUP is detected as relatively higher during Ramadan among those who are fasting for more than 12 hours daily. We suggest that people with predisposing factors should be informed before making a decision to fast.Öğe Investigation of gene expression levels in thyroid tissues of rats treated with Wi-Fi electromagnetic wave (2.4-3 GHz Wi-Fi RF-EMF)(Elsevier, 2023) Serin, Musa; Soylu, Sinan; Dastan, Sevgi Durna; Koc, Suleyman; Kurt, AtillaIn this study, it was aimed to examine the effects of wireless waves applied to rats in the thyroid tissue by evaluating gene expression levels on 12 determined gene regions. 20 healthy 16-week-old Wistar albino female rats weighting 200-220 g were used. In the experiments, two groups, control and experimental groups, were formed, and ten female rats were used in each group. While WI-FI electromagnetic field was applied to the experimental group of these rats, nothing was applied to the control group. Many different gene regions, including beta catenin, beta-actin, GAPDH, L3B, HIF 1-Alpha, GSk-3B, TCF, WNT7a, WNT10a, WNT2, Beclin 1 and Beclin 2, ATG5 and ATG12, were investigated. ACTB and GAPDH gene primers were used as a house keeping gene. The fold change values were calculated statistically from the data obtained from the gene expression re-sults. Compared to the control group, it was observed that the experimental group had significant increases in gene expression levels of many gene regions investigated in the study (P<0.05). Among the study groups, 12 different genes such as beta catenin, beta-actin, GAPDH, L3B, HIF 1-Alpha, GSk-3B, TCF, WNT7a, WNT10a, WNT2, Beclin 1 and Beclin 2, ATG5 and ATG12, which we have determined according to the literature, are known to be related to autophagy and oxidative stress. When the expression levels were investigated, it was determined that the expression coefficients of all the genes studied in the wnt/ss catenin pathway in the experimental group of rats exposed to the Wi-Fi EM field, except for ATG5 and ATG12, were quite high, and there was significant differences between the groups.Öğe Lazer Hemoroidopeksi Klinik Deneyimimiz(Sivas Cumhuriyet Üniversitesi, 2024) Genç, Hüsnü Çağrı; Çoşkun, Hakkı; Demirtaş, Yıldırımcan; Soylu, Sinan; Kurt, AtillaAmaç: Kliniğimizde uygulanan Lazer Hemoroidoplasti (LHP) işleminin sonuçlarını sunmayı amaçladık Yöntem: Bu retrospektif çalışmada, kliniğimizde 24 aylık bir dönemde yapılan LHP'nin sonuçlarını analiz ettik. Hastalar, ameliyat sonrası üç haftalık takip ziyaretleri planlandı, ardından üç ve altı aylık aralıklarla takip edildi. Bulgular: 112 hastanın 73'ü (%65.17) erkek, 39'u (%34.82) kadındı. Ortalama yaş 52.6 idi (aralık: 20-65). Ortalama operasyon süresi 18.3 dakika idi (aralık: 12-25). Yetmiş hasta grade 2 olarak sınıflandırılırken, 42 hasta grade 3 olarak sınıflandırıldı. Ortalama hastanede kalış süresi 1.16 gün idi. Grade 2 hemoroidli 70 hastanın 5'i takipten çıkarıldı, 24'ü 6 ay ve 41'i 1 yıl boyunca takip edildi. Bir (1.42%) hasta 1 yıl sonundaki takipte nüks gösterdi. Dört hasta (%9.52) nüks gösterdi. Grade 3 hemoroidli bir hasta, bir hafta süren postoperatif kanama yaşadı ve eritrosit süspansiyonu transfüzyon ihtiyacı oldu. Grade 3 hemoroidli bir başka hasta, konservatif tedavi ile çözülen bir hematom geliştirdi. Grade 3 hemoroidli sekiz hastada (%7.14) postoperatif ödem gelişti, hepsi konservatif tedavi ile iyileşti. Görsel Analog Skala (VAS) kullanılarak ölçülen ağrı değerleri, birinci postoperatif günün 2.03, ikinci postoperatif günün ise 1.49 olarak ölçüldü. Sonuç: LH tedavisi, Grade 2 ve Grade 3 hastalıklar için başarılı bir alternatif tedavi seçeneği olduğu düşünülmektedir ve LH tedavisinde hasta seçiminin kritik önem taşıdığı vurgulanmaktadır.Öğe Photodynamic activity of cationic ball-type zinc phthalocyanine in cell culture experiments with MCF-7 and MG63 cancer cells(World Scientific Publ Co Pte Ltd, 2024) Soylu, Sinan; Bagda, Efkan; Altun, Ahmet; Bagda, Esra; Kurt, Atilla; Erden, Fuat; Yabas, EbruEffective binding of cationic ball-type zinc phthalocyanine (BT-Pc) to G-quadruplex (G4) DNA molecules has already been demonstrated previously. This motivated us to investigate the PDT performance of BT-Pc in cell culture studies. Accordingly, the present work involves the application of BT-Pc to breast (MCF-7) and osteosarcoma (MG63) cancer cells, and also to healthy fibroblast cells (L929). Cell lines (CL) were irradiated at 640 nm wavelength, and the photodynamic activity was evaluated. The results show that cytotoxicity was significantly stronger in MCF-7 cells than in both L929 and MG63 cells (p < 0.05) in the absence of IPL irradiation. Also, we report that the application of l IPL provided significant photodynamic activity in MCF-7 and MG63 cells (p < 0.05). These results further suggest that BT-Pc might become a promising photosensitizer for future clinical trials.Öğe The protective effects of sildenafil in acute lung injury in a rat model of severe scald burn: A biochemical and histopathological study(ELSEVIER SCI LTD, 2013) Gokakin, Ali Kagan; Deveci, Koksal; Kurt, Atilla; Karakus, Boran Cihat; Duger, Cevdet; Tuzcu, Mehmet; Topcu, OmerSevere burn induces biochemical mediators such as reactive oxygen species that leads to lipid peroxidation which may have a key role in formation of acute lung injury (ALI). Sildenafil is a selective and potent inhibitor of cyclic guanosine monophosphate specific phosphodiesterase-5. Sildenafil preserves alveolar growth, angiogenesis, reduces inflammation and airway reactivity. The purpose of the present study was to evaluate the effects of different dosages of sildenafil in ALI due to severe scald burn in rats. Twenty-four rats were subjected to 30% total body surface area severe scald injury and were randomly divided into three equal groups as follow: control, 10 and 20 mg/kg sildenafil groups. Levels of malondialdehyde (MDA), activities of glutathione peroxidase (Gpx), catalase (Cat), total oxidative stress (TOS), and total antioxidative capacity (TAG) were measured in both tissues and serums. Oxidative stress index (OSI) was calculated. A semi-quantitative scoring system was used for the evaluation of histopatological findings. Sildenafil increased Gpx, Cat, TAG and decreased MDA, TOS and OSI. Sildenafil decreased inflammation scores in lungs. Our results reveal that sildenafil is protective against scald burn related ALI by decreasing oxidative stress and inflammation and the dosage of 10 mg/kg could be apparently better than 20 mg/kg. (C) 2013 Elsevier Ltd and ISBI. All rights reserved.Öğe Role of pentoxifylline and iloprost in the prevention of ischemia-reperfusion injury in an experimental model of intestine ischemia-reperfusion in rats(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2018) Abakay, Ugur; Soylu, Sinan; Goksel, Sabahattin; Sarac, Bulent; Inan, Zeynep Deniz Sahin; Cakmak, Erol; Korkmaz, Ozge; Kurt, Atilla; Genc, Husnu CagriBACKGROUND: Intestinal ischemia-reperfusion (I/R) injury can lead to multiple organ failure and death. The aim of this study was to investigate the effects of pentoxifylline and iloprost administered before reperfusion in intestinal ischemia. METHODS: In total, 25 male Wistar Albino rats weighing 250-300 g were divided into five groups each comprising five subjects: control group (n=5), sham group (n=5, no I/R), I/R group (n=5, 45 min ischemia, and 120 min reperfusion), I/R + pentoxifylline group (n=5, 45 min ischemia following intraperitoneal 50 mg/kg pentoxifylline and 120 min reperfusion), and I/R + iloprast group (n=5, 45 min ischemia followed by intraperitoneal 2 mcg/kg iloprost and 120 min reperfusion). At the end of the experiment, ileum specimens were stained using hematoxylin-eosin and histopathologically evaluated using the Chiu score. Isometric contraction-relaxation responses were recorded using organ baths for contraction-relaxation responses. RESULTS: Pentoxifylline provided a significant improvement in response to histopathological and contraction-relaxation responses. Although iloprost provided recovery in reperfusion injury, it was not statistically significant. CONCLUSION: Our findings demonstrate that pentoxifylline may be promising in preventing small bowel ischemia-reperfusion injury. We concluded that further clinical and experimental studies for iloprost are needed.Öğe Well-differentiated abdominal liposarcoma: experience of a tertiary care center(BIOMED CENTRAL LTD, 2015) Karadayi, Kursat; Yildiz, Caglar; Karakus, Savas; Kurt, Atilla; Bozkurt, Birkan; Soylu, Sinan; Cicekli, Ayse A.; Egilmez, Reyhan; Cetin, AliBackground: We presented abdominal liposarcoma cases diagnosed and managed in a tertiary care center and also conducted a literature review on main features of this tumor. Methods: Chart reviews of eight cases were conducted, and clinical, surgical, histopathological, and follow-up data were recorded. Results: Overall, complete surgical resection was performed with adjacent organ resection in 25% of cases, and radiotherapy was not administered. Recurrence was developed in only one case and died after 2 years and 3 months, and other cases are under follow-up without recurrence. Histopatological examinations revealed findings of well-differentiated liposarcoma. Conclusions: According to our surgical experience, the surgical margin positivity may not be a determining factor for the survival of patients with well-differentiated liposarcoma, and in the absence of macroscopic invasion, adjacent organ resection may not be required. Radiotherapy may not be preferred when complete resection of abdominal mass was achieved.
