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Öğe Clinical and histologic evaluations of experimental Acanthamoeba keratitis(SPRINGER, 2007) Polat, Zubeyde Akin; Ozcelik, Semra; Vural, Ayse; Yildiz, Esin; Cetin, AliAmoebic keratitis, a sight-threatening, progressive corneal disease, is commonly caused by ubiquitous, pathogenic, free-living Acanthamoeba spp., which are widely distributed in the environment. We investigated clinical findings and histology of Acanthamoeba keratitis in a rat cornea model. Experimental Acanthamoeba keratitis was induced in Wistar rats by intrastromal inoculation of Acanthamoeba castellanii trophozoites. The clinic features of Acanthamoeba keratitis by day 70 are observed. All rats inoculated with Acanthamoeba developed keratitis. Histologically, the eyes displayed blood vessels, edema, and amoebae in stroma. A mixed cellular response, including neutrophils, mononuclear cells, and spindle-shaped cells, was seen. In conclusion, progressive, suppurative Acanthamoeba keratitis can be induced in the rat cornea model. This rat cornea model assists researchers who study the pathogenesis of Acanthamoeba keratitis and devise treatment for this difficult condition.Öğe Dermanyssus gallinae infestation: An unusual cause of scalp pruritus treated with permethrin shampoo(INFORMA HEALTHCARE, 2010) Dogramaci, Asena Cigdem; Culha, Gulnaz; Ozcelik, SemraDermanyssus gallinae is a blood parasite of wild birds, but it is also a pest in the poultry industry. It occasionally bites mammals and thus rarely creates skin problems such as itching, papules, vesicles, and dermatitis. A 92-year-old man presented with severe itch on his head, particularly at night. He had been misdiagnosed with senile pruritus and treated with antihistamines and topical corticosteroids with temporary relief of the symptoms. On the basis of clinical and parasitological findings, D. gallinae dermatitis was diagnosed. Decontamination of the patient's immediate environment was not helpful. The patient was successfully treated using a 1% permethrin shampoo once a week for 2 weeks. During 3 months of follow-up he was free of symptoms. The case is of significance because most dermatologists have difficulty identifying ectoparasitoses, such as those that arise within new or atypical conditions.Öğe Detection of Toxoplasma gondii infection among diabetic patients in Turkey(Elsevier - Division Reed Elsevier India Pvt Ltd, 2020) Ozcelik, Semra; Alim, Mehtap; Ozpinar, NecatiBackgound: In some recent studies, it has been suggested that there may be a relationship between Toxoplasma gondii (T. gondii) and type 2 diabetes mellitus (T2DM). T. gondii is of greater significance in these patients. Objectives: In the present study, it was aimed to investigate the seroprevalence of T. gondii in T2DM patients. Methods: To define Toxoplasma IgG and IgM seropositivity and determine the seroprevalence of toxoplasmosis in patients presenting at the Internal Diseases outpatient clinic of the Healthcare Application and Research Hospital, where the ELISA method was used. 200 T2DM patients and 100 healthy individuals with no complaints took part in the study. Results: Toxoplasma IgG positivity was determined in 53% of the 200 T2DM patients and Toxoplasma IgM positivity in 13%. While in the control group, Toxoplasma IgG positivity was determined in 27% and Toxoplasma IgM positivity in 1%. The risk of toxoplasmosis infection in T2DM patients was two folds higher than healthy controls according to the IgG results. IgM results were further differentiated. Conclusions: We believe that the seroprevalence of T. gondii is high in the T2DM patient group and that the causes should be investigated in more detail.Öğe Genotyping of Giardia intestinalis isolated from people living in Sivas, Turkey(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2012) Degerli, Serpil; Degerli, Naci; Celiksoz, Ali; Ozcelik, SemraAim: The technique of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) genotyping was used to characterise morphologically identical isolates of Giardia intestinalis from human stool samples. Materials and methods: In this study a total of 17 trophozoite samples, obtained either directly from stool samples or after excystation, or by duodenal aspiration, were used. A set of primers was chosen to amplify the different regions of triose phosphate isomerase (tpi) and a segment of the glutamate dehydrogenase (gdh) genes. A single-stranded conformational polymorphism technique was also used in an attempt to discriminate among some subgroups. Results: Only primers of the 683-bp segment of the tpi gene from the trophozoite samples were suitable for obtaining a PCR product. In the total of 17 trophozoite DNAs where the tpi gene segment was amplified, 9 belonged to assemblage A (53%) and 4 to assemblage B (23.5%). It was not possible to identify assemblages for the remaining 4 samples (23.5%). Conclusion: PCR RFLP tpi gene application was able to discriminate between G. intestinalis assemblage A and B, but not the other subgroups. Since assemblage A is the more prevalent subgroup compared with assemblage B, this subgroup can be said to be responsible for common Giardia infections in Turkey.Öğe The Investigation of Cryptosporidium spp. Prevalence in Cattle and Farmers by ELISA(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2012) Ozcelik, Semra; Poyraz, Omer; Kalkan, Kadir; Malatyali, Erdogan; Degerli, SerpilCryptosporidium spp. is an important cause of enteritis for animals and humans. As the parasite has zoonotic potential, it is aimed to investigate the prevalence in people who had close contact with livestock, in control group who lived in Centrum and cattle. Fecal samples collected from 50 farmers who lived in different villages of Sivas and 65 people to be control group lived in Sivas Center without gastrointestinal discomfort and 200 cattle and calf breeding different county during the period from September 2009 to September 2010. Fecal samples were stored in 10% formalin until examination and then studied with Cryptosporidium Stool Antigen Microwell ELISA Kit. As a result, positive rate were found 4(6.2%) in control group and 9(18%) in study group, (chi(2)=3.95, P<0.05). On the other hand, 15 (7.5%) of the cattle and calve samples gave positive reaction with Cryptosporidium spp. ELISA.Öğe Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey(HINDAWI PUBLISHING CORPORATION, 2013) Coskun, Kubra Acikalin; Ozcelik, Semra; Tutar, Lutfi; Elaldi, Nazif; Tutar, YusufThe present work focuses on a local survey of free-living amoebae (FLA) that cause opportunistic and nonopportunistic infections in humans. Determining the prevalence of FLA in water sources can shine a light on the need to prevent FLA related illnesses. A total of 150 samples of tap water were collected from six districts of Sivas province. The samples were filtered and seeded on nonnutrient agar containing Escherichia coli spread. Thirty-three (22%) out of 150 samples were found to be positive for FLA. The FLA were identified by morphology and by PCR using 18S rDNA gene. The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of three different species, Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis. Naegleria fowleri, Balamuthia mandrillaris, or Sappinia sp. was not isolated during the study. All A. castellanii and A. polyphaga sequence types were found to be genotype T4 that contains most of the pathogenic Acanthamoeba strains. The results indicated the occurrence and distribution of FLA species in tap water in these localities of Sivas, Turkey. Furthermore, the presence of temperature tolerant Acanthamoeba genotype T4 in tap water in the region must be taken into account for health risks.Öğe The Prevalence of Pediculus humanus capitis and the Coexistence of Intestinal Parasites in Young Children in Boarding Schools in Sivas, Turkey(WILEY-BLACKWELL, 2012) Degerli, Serpil; Malatyali, Erdogan; Celiksoz, Ali; Ozcelik, Semra; Mumcuoglu, Kosta Y.The aim of this study was to investigate the prevalence of Pediculus humanus capitis and the coexistence of intestinal parasites in boarding primary schools in Sivas, Turkey. Seven hundred seventy-two students (350 [45.3%] girls, 422 [54.7%] boys) were evaluated with combing for the presence of head lice, collection of fecal samples, and examination of the perianal region for intestinal parasites using the cellophane tape method. The overall infestation rate for head lice was 6% (n = 46). Nine children had evidence of nits only (1.2%), whereas living lice and nits or eggs were found in 37 children (4.8%). Girls were significantly more commonly infested (12.9%) than boys (0.2%). Of the parameters evaluated, socioeconomic level, number of rooms per family, and size and weight of the children were statistically significantly different between the children with and without lice. Although the infestation rate of children with intestinal parasites was higher in the head louse-infested group (23.9%) than in the group of children without lice (17.6%), the differences were not statistically significant.Öğe Search of house mite's fauna and investigation of relationship between house dust mite and allergy in the province of Hatay, Turkey(Kuwait Medical Assoc, 2019) Gulkan, Burcu; Degerli, Serpil; Culha, Gulnaz; Savas, Nazan; Ozcelik, SemraObjective: This study aims to search house mite's fauna and to investigate the relationship between house dust mite prevalence and house-related risk factors and allergic diseases. Design: Cross sectional study Setting : Two hundred houses in 25 different neigborhoods in Hatay province of Turkey. Hatay province is located in East Mediterranean Region with a population of 1,519,836. It has the border with Syria. The sample was selected by clustered sociodemographic region systematic random sampling method. Subjects: Two hundred samples of house dust Intervention: A survey was used. Pearson Chi-Square test was used. Main outcome measure(s): Samples of house dust in total were analyzed according to Spieksma-Boezman method of lactic acid precipitation; and the identification of the samples in which mites were detected was made according to the Coloff's type identification key. Results: Out of 200 samples of house dust, mite was detected in 57.5% of them. The most frequently found type of mite was determined as D. pteronyssinus (72.2%). There was no significant relationship found between the mite prevalence and other criteria, except the number of rooms when the ground clearance of houses, the case of garden, carpet type, washing temperature and duration of duvets, times of the insolation of pillows and quilts, heating devices, cleaning times, insolation conditions, presence of a pet and the economic situation of the family were analyzed (p > 0.05). Conclusion It is determined that mite prevalence in the province of Hatay, Turkey is higher than many regions.Öğe Search of house mite’s fauna and investigation of relationship between house dust mite and allergy in the province of Hatay, Turkey(Kuwait Medical Association, 2019) Gulkan, Burcu; Degerli, Serpil; Culha, Gulnaz; Savas, Nazan; Ozcelik, SemraObjective: This study aims to search house mite’s fauna and to investigate the relationship between house dust mite prevalence and house-related risk factors and allergic diseases. Design: Cross sectional study Setting: Two hundred houses in 25 different neigborhoods in Hatay province of Turkey. Hatay province is located in East Mediterranean Region with a population of 1,519,836. It has the border with Syria. The sample was selected by clustered sociodemographic region systematic random sampling method. Subjects: Two hundred samples of house dust Intervention: A survey was used. Pearson Chi-Square test was used. Main outcome measure(s): Samples of house dust in total were analyzed according to Spieksma-Boezman method of lactic acid precipitation; and the identification of the samples in which mites were detected was made according to the Coloff’s type identification key. Results: Out of 200 samples of house dust, mite was detected in 57.5% of them. The most frequently found type of mite was determined as D. pteronyssinus (72.2%). There was no significant relationship found between the mite prevalence and other criteria, except the number of rooms when the ground clearance of houses, the case of garden, carpet type, washing temperature and duration of duvets, times of the insolation of pillows and quilts, heating devices, cleaning times, insolation conditions, presence of a pet and the economic situation of the family were analyzed (p >0.05). Conclusion It is determined that mite prevalence in the province of Hatay, Turkey is higher than many regions. © 2019, Kuwait Medical Association. 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