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Öğe Alendronate enhances osseous healing in a rat calvarial defect model(PERGAMON-ELSEVIER SCIENCE LTD, 2012) Toker, Hulya; Ozdemir, Hakan; Ozer, Hatice; Eren, KayaAim: The aim of this study was to evaluate the effect of alendronate on osseous wound healing in an experimental model. Methods: Critical size defects were created in calvaria of 40 male Wistar rats. The animals were divided into four groups of 10 animals each: autogenous bone graft group; autogenous bone graft with systemic alendronate group (0.01 mg /kg body weight per day for 8 weeks); autogenous bone graft with local alendronate group (1 mg/mL for 5 min); non-treatment (control) group. Animals were sacrificed after 8 weeks; osteoblast number, lamellar bone formation, and area of newly formed bone were analysed. Results: The osteoblast number significantly increased in the autogenous bone graft with local alendronate group compared to the autogenous bone graft group (p < 0.05). Both systemic and local application of the alendronate significantly increased the new bone formation compared to the autogenous bone graft group (p < 0.05) with no significant difference between local or systemic use (p > 0.05). Local alendronate and autogenous bone graft use significantly increased the total bone area compared to autogenous bone graft alone (p < 0.05). Conclusion: Alendronate enhances the new bone formation by autogenous bone graft in the rat calvarial defect model suggesting that the inhibition of the osteoclastic activity allows an increased rate of bone apposition, which could be applicable to the inflammation-induced destruction of the periodontal tissues during disease. (C) 2012 Elsevier Ltd. All rights reserved.Öğe Clinical Evaluation of Autologous Platelet-Rich Fibrin in the Treatment of Multiple Adjacent Gingival Recession Defects: A 12-Month Study(QUINTESSENCE PUBLISHING CO INC, 2015) Tunali, Mustafa; Ozdemir, Hakan; Arabaci, Taner; Gurbuzer, Bahadir; Pikdoken, M. Levent; Firatli, ErhanLeukocyte- and platelet-rich fibrin (L-PRF) belongs to a new generation of platelet concentrates. There are limited numbers of studies focused on the use of L-PRF in gingival recession defects. This study evaluated the safety and effectiveness of using L-PRF membranes as a substitute for free connective tissue grafts (CTGs) as a treatment method for gingival recession defects. A total of 44 Miller Class I/II gingival recessions that were bilateral, adjacent, and greater than 3 mm in size were selected. Each recession site was randomly assigned to the test group (L-PRF) or the control group (CTG). After 12 months, root coverage was 76.63% and 77.36% in the L-PRF and CTG groups, respectively. It is suggested that L-PRF membrane may be an alternative graft material for treating multiple adjacent recessions greater than 3 mm in size without a requirement for additional surgery.Öğe A comparative evaluation of the systemic and local alendronate treatment in synthetic bone graft: a histologic and histomorphometric study in a rat calvarial defect model(ELSEVIER SCIENCE INC, 2012) Toker, Hulya; Ozdemir, Hakan; Ozer, Hatice; Eren, KayaObjective. The purpose of this study was to compare the relative efficacy of systemic and local alendronate treatment of synthetic bone graft in a rat calvarial defect model. Study Design. Forty Wistar rats were divided into 4 groups: experimental animals received alendronate systemically or locally combined with micro-macroporous biphasic calcium phosphate (MBCP) graft material. In the control group, the defect was left empty. On each animal, a 5-mm standardized bone defect was created with a standard trephine bur in calvarium. All animals were killed after 8 weeks. The number of osteoclasts, osteoclast morphology, resorption lacunae, osteoblastic activity, and lamellar bone formation were histopathologically evaluated and the newly formed bone area was analyzed histomorphometrically. Results. Eight weeks after surgery, the number of osteoclasts and the resorption lacunae in the MBCP group using systemic alendronate therapy was significantly higher than those of the other groups (P < .05). Osteoblast number in the MBCP group using systemic alendronate treatment was significantly increased (P < .05). No significant difference was found among all MBCP groups using local or systemic alendronate treatments with regard to new bone formation (P > .05). Conclusions. Within the limits of the study, alendronate, when administered systemically or locally, did not increase bone regeneration with MBCP graft in the rat calvarial defect model. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114(suppl 5):S146-S152)Öğe Dramatic oral findings belonging to a pica patient: A case report(WILEY, 2009) Toker, Hulya; Ozdemir, Hakan; Ozan, Fatih; Turgut, Mehmet; Goze, Fahrettin; Sencan, Mehmet; Kantarci, AlpdoganPica is a compulsive eating of non-nutritive substances and can have serious medical implications. The causes are not known, but the symptom is often associated iron-deficiency. Pica is an under-diagnosed problem. Although few dental problems associated with pica have been reported, because of its potential impact on blood measures, dental professionals must be aware of this common, but commonly missed, condition. This is an unusual case of a patient diagnosed with aggressive periodontitis (AgP) who had a dirt eating habit (geophagia) and was diagnosed as having pica.Öğe The effect of boron on alveolar bone loss in osteoporotic rats(ELSEVIER TAIWAN, 2016) Toker, Hulya; Ozdemir, Hakan; Yuce, Hatice Bald; Goze, FahrettinBackground/purpose: The aim of this study is to investigate the effects of systemically administered boric acid on osteoporosis-related bone alterations, alveolar bone loss, receptor activator of nuclear factor kappa-b ligand (RANKL) expressions, and mandibular bone density in experimental periodontitis model in osteoporotic rats. Materials and methods: Thirty-six male Wistar rats were separated into five study groups: non-ligated control (C, n = 6) group; periodontitis (P, n = 6) group; osteoporosis (0, n = 8) group; osteoporosis + periodontitis n = 8) group, and osteoporosis + periodontitis with 50 mg/kg/d boric acid (BA50, n = 8) group for 15 days. Osteoporosis was created with intraperitoneal injection of 80 mg/kg retinoic acid for 15 days. Silk ligatures (4/0) were placed around the mandibular right first molar teeth to induce experimental periodontitis. After induction of osteoporosis and periodontitis, rats were sacrificed at Day 15. Alveolar bone loss was evaluated with a stereomicroscope by measuring the distance from the cement-enamel junction to the alveolar crest. Density measurements were performed on radiographs. RANKL and tartrate resistant acid phosphatase (TRAP) staining were performed on histological slides. Results: Alveolar bone loss was significantly higher in the O+P group than those of the other groups (P < 0.05). Boric acid decreased bone loss (P < 0.05). TRAP + osteoclast numbers were highest in the P group and lowest in the control group. The differences in TRAP + osteoclast numbers among control, P, O+P, and BA50 groups were significant (P < 0.05). There were no significant differences in RANKL expression and mandibular bone density (P > 0.05). Conclusion: Within limitations of this study, we conclude that boric acid may decrease alveolar bone loss in a rat model with periodontitis and osteoporosis. Copyright (C) 2016, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license.Öğe Effect of Chronic Periodontitis on Serum and Gingival Crevicular Fluid Oxidant and Antioxidant Status in Patients With Familial Mediterranean Fever Before and After Periodontal Treatment(AMER ACAD PERIODONTOLOGY, 2014) Bostanci, Vildan; Toker, Hulya; Senel, Soner; Ozdemir, Hakan; Aydin, HuseyinBackground: The aim of this study is to investigate the impact of periodontal status on oxidant/antioxidant status in patients with chronic periodontitis (CP) who experienced familial Mediterranean fever (FMF) and their response to non-surgical periodontal therapy. Methods: Data were obtained from 13 patients with FMF with generalized CP (FMF-CP), 15 systemically healthy patients with generalized CP, 15 systemically and periodontal healthy controls (HCs), and 14 periodontally healthy patients with FMF (FMF-HC). Each participant's total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in their gingival crevicular fluid (GCF) and serum were recorded. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks after periodontal treatment were recorded. Results: The study showed statistically significant improvement of clinical parameters in both FMF-CP and CP groups after periodontal treatment. The baseline GCF-TOS and OSI levels were significantly higher in the CP group compared with the FMF-CP group (P < 0.05). After periodontal treatment, the GCF-TOS levels were significantly reduced in members of the FMF-CP group (P < 0.05). The GCF-TAS levels in members of the FMF-CP group were significantly higher than those of members of the HC group at baseline (P < 0.05). Serum-TAS levels in the FMF-CP group were significantly higher than those in the CP and HC groups at baseline (P < 0.05). The GCF-TOS level in the FMF-CP group was significantly higher than that in the FMF-HC group at baseline and 6 weeks. However, there were no significant differences in the serum-TOS and serum-OSI levels of those in the FMF-CP and CP groups at baseline and 6 weeks (P > 0.05). Conclusion: The results of the present study show that patients with FMF-CP displayed reduced oxidative stress and increased antioxidant status compared with those in the CP and HC groups.Öğe Effect of different mouth rinses on third molar surgery-related oral malodor(MOSBY-ELSEVIER, 2008) Polat, Hidayet Burak; Ozdemir, Hakan; Ay, SinanObjective. The purpose of this study was to assess the effects of 4 types of widely used commercial mouth rinses on third molar surgery-related oral malodor. Study design. In this double-blind selective clinical trial, 80 participants (40 women, 40 men) who had undergone third molar surgery were divided into 5 groups, and different mouth rinses were given to each: 0.2% chlorhexidine gluconate (Chx), 0.12% chlorhexidine gluconate with 0.15% benzydamine hydrochloride (Chx + Bzd), 7.5% polyvinylpyrrolidone iodine (Pvp), 0.15% benzydamine hydrochloride (Bzd), and sterile saline solution (Ss), with other routine medications. Oral malodor of patients was evaluated with 3 methods; using a Halimeter, an organoleptic method, and patient self-evaluation. Measurements were performed preoperatively and postoperatively (pre-op, third, eighth, and fifteenth days). Results. Bad breath parameters systematically increased (P < .05) in all groups after third molar surgery on the third and eighth days. The Pvp and Ss groups showed higher scores when volatile sulfur compounds were considered. When organoleptic and patient self-evaluation scores were considered, the Bzd and Ss groups had higher scores than the others on the third and eighth days. The difference between the pre-op day and the fifteenth day was not significant in all groups in terms of all measures. Conclusion. Third molar surgery-related oral malodor increases during the first postoperative week and decreases to the preoperative level after 15 days. Results from the 3 different methods showed that Chx and Chx + Bzd mouth rinses are more effective mouth rinses than the others on third molar surgery -related oral malodor.Öğe Effect of external tooth bleaching on dental plaque accumulation and tooth discoloration(MEDICINA ORAL S L, 2008) Gursoy, Ulvi Kahraman; Eren, Digdem Isin; Bektas, Ozden Ozel; Hurmuzlu, Feridun; Bostanci, Vildan; Ozdemir, HakanObjectives: Treatment of dental discolorations with external bleaching is becoming very common in dentistry, however, possible irreversible alterations on enamel surface due to bleaching procedures is a topic of discussion. The aim of this study was to evaluate the clinical effects of bleaching by measuring the dental plaque accumulation on human enamel and tooth discoloration in vivo. Study Design: Forty-four teeth in eleven patients not revealing any restorations or periodontal problems were enrolled in this study. Bleaching agent applied only to labial surfaces of incisors using commercial 35 % hydrogen peroxide gel. Dental plaque and tooth color measured in the same group of participants, at the end of non-brushing periods lasting 3 and 5 days, respectively, before and after bleaching. Results: The results of the comparison of pre- and post-bleaching measurements showed that, after a non-brushing period lasting 3 day, discoloration scores and plaque accumulation scores for bleached surfaces were lower than the non-bleached surface scores. However, at the end of a non-brushing period lasting 5 day, even the color measurement score in post-bleaching period was lower than the pre- bleaching counterpart, plaque index measurements showed higher plaque accumulation scores in the bleached group. Conclusions: According to these results, bleaching with 35 % hydrogen peroxide seem to favor plaque accumulation after non-brushing period lasting 5 day and tooth discoloration after bleaching is not in correlation with the amount of plaque accumulation.Öğe Effect of fixed appliances on oral malodor(MOSBY-ELSEVIER, 2011) Babacan, Hasan; Sokucu, Oral; Marakoglu, Ismail; Ozdemir, Hakan; Nalcaci, RuhiIntroduction: The objective of this study was to evaluate the effect of fixed appliances on oral malodor. Methods: Forty-one patients participated in this study. They were randomized into 2 groups: treated with fixed orthodontic appliances and untreated controls. Oral malodor measurements and the gingival and plaque indexes were recorded in each group by same periodontist. Measurements were taken from the study group before bonding, 1 week after bonding, and 4 weeks after bonding. The control group's measurements were made at the same time. Results: Oral malodor and the gingival and plaque indexes were significantly increased 1 week after bonding (P < 0.05). Oral malodor was increased at 4 weeks after bonding (P > 0.05). The comparison of the groups showed differences 1 week and 4 weeks after bonding. Conclusions: The oral malodor reached the critical level during fixed orthodontic treatment. Oral malodor could indicate a need to evaluate oral health and remind patients of the importance of maintaining ideal oral hygiene. (Am J Orthod Dentofacial Orthop 2011;139:351-5)Öğe Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model(SPANDIDOS PUBL LTD, 2017) Ezirganli, Seref; Kazancioglu, Hakki Oguz; Acar, Ahmet Huseyin; Ozdemir, Hakan; Kuzu, Emre; Inan, Deniz SahinBone defects resulting from oncological surgical resections, congenital facial anomalies, trauma or infection represent a significant and common clinical problem. The present study aimed to evaluate the effects of a commercially-available medicinal plant extract product, Ankaferd BloodStopper (ABS), on bone healing. The present study was performed on 24 female ovariectomized (OVX) rats. A defect on each rat calvarium was created using a trephine burr prior to the rats being divided into two groups. Defects were grafted with a gelatin sponge soaked with normal saline (control group) or plant extract (experimental group). Half of the animals were sacrificed after 2 weeks and the others after 4 weeks. In the control group, the defects were not filled with regenerated bone. By contrast, in the experimental group, all defect areas had an increased amount of regenerated bone and connective tissue. Osteoblastic activity appeared to be greater in the experimental group however, osteoclastic activity was observed to be higher in the control group. At 2 and 4 weeks, there was a significant difference in the amount of newly regenerated bone observed in the experimental group compared with the control group (P<0.05). Therefore, the results of the present study indicated that local ABS application had a positive effect on bone healing in the OVX rat model.Öğe Effects of boric acid on experimental periodontitis and alveolar bone loss in rats(PERGAMON-ELSEVIER SCIENCE LTD, 2012) Demirer, Serhat; Kara, M. Isa; Erciyas, Kamile; Ozdemir, Hakan; Ozer, Hatice; Ay, SinanObjective: The goal of the present study was to evaluate the histopathologic and morphometric effects of systemic boric acid in a rat periodontitis model. Design: Twenty-four Wistar rats were divided into three groups of eight animals each: non-ligated (NL), ligature only (LO), and ligature and treated with boric acid (BA) (3 mg/kg per day for 11 days). A 4/0 silk suture was placed in a subgingival position around the mandibular first molars; after 11 days the rats were sacrificed, and changes in alveolar bone levels were measured clinically and tissues were histopathologically examined to assess the differences amongst the study groups. Results: The ratio of presence of inflammatory cell infiltration (ICI) and osteoclast number in the LO group was significantly higher than that of the NL and BA groups (p < 0.05). The ratio of presence of osteoblastic activity in the LO group was significantly lower than that of the NL and BA groups (p < 0.05). Alveolar bone loss was also significantly higher in the LO group compared to the BA and NL groups (p < 005). Conclusions: This study has demonstrated that systemic administration of boric acid reduced periodontal inflammation and alveolar bone loss in periodontal disease in rats. (C) 2011 Elsevier Ltd. All rights reserved.Öğe Effects of Debonding on Oral Malodor(Aves Press Ltd, 2009) Sokucu, Oral; Ozdemir, Hakan; Nalcaci, Ruhi; Marakoglu, Ismail; Babacan, HasanThe objective of this study was to evaluate the affect of debonding on oral malodor. This study consists of three groups. The first group (debonding group) included 20 subjects with brackets and would be debonded in a month. The second group ( bracket group) included 20 subjects who still undergone orthodontic treatment with brackets. The last group is a control group who did not receive orthodontic treatment. Oral malodor measurements, Gingival and Plaque Index scores were recorded in each group by same periodontist. Measurements were taken in first study groups before debonding (T1), 1 week after debonding ( T2) and 4 weeks after bonding (T3). At the same time the measurements were applied to the second and control group. Data's were evaluated with three way ANOVA test. Bonferroni test were assessed when significant differences between the measurements were exist. Mann-Whitney U- test was used to compare both groups. Oral malodor, significantly decreased after debonding ( P< 0.05). One month after debonding the parameters were stable on debonding group and bracket group. ( P> 0.05). Control group did not show differences in all measurements. The comparison between groups showed significant differences between groups (P< 0.05). Bonding brackets cause to increase on oral malodor. However, after debonding immediately oral malodor reached to the acceptable scores. Oral malodor could be another indicator to evaluate the oral health as Gingival and Plaque index scores.Öğe The Effects of Nigella Sativa Seed Extract on Bone Healing in an Experimental Model(LIPPINCOTT WILLIAMS & WILKINS, 2016) Ezirganli, Seref; Kazancioglu, Hakki Oguz; Ozdemir, Hakan; Inan, Deniz Sahin; Tek, MustafaThe purpose of this study was to histologically evaluate the effects on bone healing of nigella sativa seed extract applied on calvarial defects in an ovariectomized rat model. The study included 32 female rats weighing 280 to 310g with an average age of 3 months. A defect was created with a trephine burr on each rat calvarium. The rats were divided into 2 groups (control and study) of 8 animals each. All the defects were grafted with a gelatin sponge mixed with normal saline. In the study group, nigella sativa seed extract was applied systemically using an oro-gastric tube. Half of the animals in each group were sacrificed after 2 weeks, and the others after 4 weeks. In the control groups, the defects were not completely filled with regenerated bone. Osteoblast cells were observed more in the study groups. A higher rate of osteoclasts was determined in the control groups. In addition, the nigella sativa group had a statistically greater amount of bone formation than the others group at both 2 weeks and 4 weeks (P<0.05). The systemic application of nigella sativa seed extract demonstrated incredibly positive effects on enhanced bone healing in this experimental osteoporotic model.Öğe The effects of non-surgical periodontal therapy on oxidant and anti-oxidant status in smokers with chronic periodontitis(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Akpinar, Aysun; Toker, Hulya; Ozdemir, Hakan; Bostanci, Vildan; Aydin, HuseyinAim: The aim of this study was to determine the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) and serum oxidant-antioxidant levels in smoking and non-smoking patients with chronic periodontitis. Methods: Twenty-nine patients with chronic periodontitis (15 smokers (CP-S) and 14 nonsmokers (CP-NS)) and 20 periodontally healthy subjects (10 smokers (H-S) and 10 non-smokers (H-NS)) totalling 49 subjects were included in this study. GCF was collected from at least two pre-selected sites (one moderate and one deep pocket) in patients with CP. In the healthy group, GCF samples were collected from one site. Probing pocket depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. To determine serum total oxidant status (TOS) and total antioxidant status (TAS), venous blood was drawn from each subject. The GCF, serum sampling, and clinical measurements were recorded at baseline and 6 weeks after periodontal treatment. Results: The study showed statistically significant improvement of clinical parameters after periodontal treatment in both smokers and non-smokers. In the CP-S group, there were no significant differences in GCF TAS levels at both moderate and deep pocket sites between baseline and 6 weeks (p > 0.05). GCF TAS levels in the CP-NS groups were significantly increased (p < 0.05) at moderate and deep pocket sites between baseline and 6 weeks. GCF TOS levels in the CP-S groups were significantly decreased (p < 0.05) at deep pocket sites between baseline and 6 weeks. There was no significant difference in serum TAS levels of the all periodontitis patient groups between at baseline and 6 weeks (p > 0.05). Serum TOS levels in the CP-S and CP-NS groups were significantly decreased (p < 0.05) after periodontal treatments. Conclusions: The periodontal treatment improves the clinical parameters in both smokers and non-smokers. These results confirm that non-surgical periodontal therapy can reduce oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.Öğe Effects of Orthodontic Treatment on Patients' Dental Anxiety and Oral Health Knowledge(Aves Press Ltd, 2009) Ozturk, Firat; Sokucu, Oral; Demirer, Serhat; Nalcaci, Ruhi; Ozdemir, HakanThe aim of this study was to investigate effects of orthodontic treatment on both anxiety and oral health knowledge of orthodontic patients. The study was carried out in 184 patients. Four groups were formed according to the stage of orthodontic treatment. Group 1 consisted of 47 individuals selected from patients who were awaiting orthodontic treatment; groups 2 and 3, comprised 44 and 46 individuals who had been undergoing orthodontic treatment for a period of 1-12 and 12-26 months respectively, and group 4, 47 orthodontically treated individuals. The modified Dental Anxiety Scale was used to measure dental anxiety. Oral health knowledge was assessed using several questions concerning etiology, symptoms and prevention. Chi-squire test was used for statistical assessment. The anxiety level of patients in group 1 was significantly higher than the subjects in group 4. Patients in groups 2, 3 and 4 mostly knew that gum bleeding is a sign of periodontal disease (81.8, 84.7, 80.1 per cent respectively); and this was 63.8 per cent for group 1. Patients in all groups were aware how to prevent, however those who were undergoing or had completed treatment were more informed about symptoms of periodontal disease. Only 19.6 and 52.1 per cent of subjects in group 3 had the knowledge to identify dental plaque and what it can cause respectively; which was the highest ratio among the groups. Orthodontic treatment involves some degree of exposure, consequently creates a positive influence on patients' anxiety and periodontal health knowledge.Öğe Effects of platelet rich fibrin alone used with rigid titanium barrier(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Ozdemir, Hakan; Ezirganli, Seref; Kara, M. Isa; Mihmanli, Ahmet; Baris, EmreObjective: Platelet-rich fibrin (PRF) is a platelet and leukocyte rich and platelet preparation that concentrates various growth factors and therefore has the potential to be used as regenerative treatment. The aim of study was to assess the effects of platelet rich fibrin (PRF) on bone augmentation when used in conjunction with titanium barrier a rabbit calvaria model. Study design: Twenty-four adult male New Zealand rabbits were used in this study. Two titanium barriers were fixed on each rabbit's calvarium. The rabbits were divided into four groups (group one is control and the other three groups are experimental) and each group contains 6 animals. PRF, anorganic bovine bone (ABB), and biphasic calcium phosphate (BCP) were used with titanium barriers in the experimental groups. Any materials were not used in the control group. Half of the animals were sacrificed after 1 month, and the rest were sacrificed after 3 months. Histomorphometric evaluation was carried out in order to compare new bone formation among the groups. Results: Significantly more new bone area was noted in the PRF alone group than in the control group, no statistically significant differences were found among PRF, BCP and ABB groups after 1 month. PRF and ABB also had superior effects in new bone formation area control to the BCP group after 3 months. Conclusion: PRF may offer the ease of use, simple handling, and enhanced delivery of growth factors during the bone augmentation procedures. When used in conjunction with the titanium barriers, PEP use can increase the quality of the newly formed bone and enhance the rate of bone formation due to the concentration of growth factors. (C) 2012 Elsevier Ltd. All rights reserved.Öğe In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate(CHURCHILL LIVINGSTONE, 2013) Tunali, Mustafa; Ozdemir, Hakan; Kucukodaci, Zafer; Akman, Serhan; Firatli, ErhanWe have developed a new, titanium-prepared, platelet-rich fibrin (T-PRF) together with the protocol for forming it, which is based on the hypothesis that titanium tubes may be more effective at activating platelets than the glass tubes used by Chouckroun in his platelet-rich fibrin (PRF) method. The aim of this study was to find a suitable animal model in which to evaluate the method and to investigate the efficacy of T-PRF for wound healing. Blood samples from 6 rabbits were used to confirm the protocol for formation of T-PRF. We evaluated T-PRF or T-PRF-like clots morphologically using scanning electron microscopy (EM). Blood samples from 5 rabbits were used to develop an experiment in which to evaluate the effects of T-PRF on wound healing. The mucoperiosteal flaps were filled with autologous T-PRF membranes from the vestibule in the anterior mandibular regions. Samples collected from the surgical sites were stained with haematoxylin and eosin. We found a mature fibrin network in T-PRF clots that had been centrifuged for 15 min at 3500 rpm and, 15 days after placement of the membrane, we found newly-forming connective tissue and islets of bony tissue in the T-PRF membrane. These results show that T-PRF could induce the formation of new bone with new connective tissue in a rabbit model of wound healing within 30 days of treatment. Published by Elsevier Ltd. on behalf of The British Association of Oral and Maxillofacial Surgeons.Öğe Klippel-Trenaunay Syndrome Manifesting as Gingival Overgrowth and Teeth Agenesis(JOURNAL PEDODONTICS INC, 2010) Ozdemir, Hakan; Marakoglu, Ismail; Akyol, Melih; Goze, O. Fahrettin; Gursoy, Ulvi KahramanObjective: Klippel-Trenaunay syndrome (KTS) is characterized by triad of venous varicosity, naevus flammeus, and soft/hard tissue hypertrophy. Manifestations of the syndrome in the head and neck region are rare, but in some cases hemangioma of the lips, tongue, and gums, open bite and cross bite, and early tooth eruption are associated with the disease. Study design: We report a 12-year-old KTS patient with gingival hyperplasia, congenital missing teeth, and increased mucosal vascularization as oral manifestations. Results and conclusion: All manifestations of the KTS were observed on the same side of the head, except bilateral missing teeth. Histological examination showed several vascular enlargements in enlarged gingiva. It is suggested that oral manifestations of the syndrome are generally related to the severity of the disease, but they do not always present in the same pattern.Öğe A morphometric and histopathologic evaluation of the effects of propolis on alveolar bone loss in experimental periodontitis in rats(AMER ACAD PERIODONTOLOGY, 2008) Toker, Hulya; Ozan, Fatih; Ozer, Hatice; Ozdemir, Hakan; Eren, Kaya; Yeler, HasanBackground: Propolis collected by honeybees from various plant sources is a resinous hive product possessing a broad spectrum of biologic activities. Propolis has been used extensively in the diet to improve health and prevent disease. The purpose of this study was to analyze the morphometric and histopathologic changes associated with experimental periodontitis in rats in response to the systemic administration of propolis. Methods: Forty Wistar rats were divided into four experimental groups: non-ligated (NL; IN = 10); ligature only (LO; N = 10); and systemic administration of ligature and propolis (100 mg/kg body weight per day [Pro100; N = 10] or 200 mg/kg body weight per day [Pro200; N = 10]). Silk ligatures were placed at the gingival margin of the lower first molars in both mandibular quadrants. The study duration was 11 days, and the animals were sacrificed at the end of this period. Changes in alveolar bone levels were clinically measured, and tissues were histopathologically examined to assess the differences among the study groups. Results: At the end of 11 days, alveolar bone loss was significantly higher in the LO group compared to the NL, Pro 100, and Pro200 groups (P<0.05). Osteoclast numbers in the LO group were significantly higher than those of the NL, Pro 100, and Pro200 groups (P<0.05). Both dosages of propolis significantly reduced the periodontitis-related bone loss, but the differences between the two propolis groups were not statistically significant (P>0.05). Conclusion: The findings of this study provide morphologic and histologic evidence that propolis, when administered systemically, prevents alveolar bone loss in the rat model.Öğe N-Acetylcysteine, a Thiol Antioxidant, Decreases Alveolar Bone Loss in Experimental Periodontitis in Rats(AMER ACAD PERIODONTOLOGY, 2009) Toker, Hulya; Ozdemir, Hakan; Eren, Kaya; Ozer, Hatice; Sahin, GonulBackground: The purpose of this study was to analyze the morphometric and histopathologic changes associated with experimental periodontitis in rats in response to systemic administration of N-acetylcysteine (NAC). Methods: Forty-three Wistar rats were divided into five experimental groups: non-ligated (NL) group (n = 10), ligature only (LO) group (n = 10), and groups that were administered NAC systemically (7, 35, or 70 mg/kg body weight per day [NAC7, NAC35, and NAC70 groups, respectively]; n = 8, 9, and 6). Silk ligatures were placed at the gingival margin of the lower first molars in a mandibular quadrant. The study duration was 11 days, and the animals were sacrificed at the end of this period. Changes in alveolar bone levels were measured clinically and tissues were histopathologically examined to assess the differences among the study groups. Results: At the end of 11 days, the alveolar bone loss was significantly higher in the LO group compared to NL, NAC7, NAC35, and NAC70 groups (P<0.05). There was no statistically significant difference in the osteoclast numbers among the study groups (P>0.05), whereas the effect of NAC was dose-dependent. Conclusion: NAC prevented alveolar bone loss in the rat model, in a dose-dependent manner, when administered systemically. J Periodontol 2009;80:672-678.
