Yazar "Ozsensoy, Yusuf" seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Assessment of polymorphism on kappa-casein gene of Anatolian water buffalo breed using the PCR-RFLP method(Tubitak Scientific & Technological Research Council Turkey, 2020) Ozsensoy, YusufWater buffalo breeding is practiced in various provinces of Turkey, and is also included in the National Breeding Project. It was aimed in this study to investigate polymorphisms on exon 4-intron 5 and exon 4 only of the kappa-casein (CSN3) gene for milk yields of water buffalo bred in Sivas Province. Blood samples were taken from 135 water buffaloes. The phenol/chloroform method for DNA extraction was conducted on collected blood. Polymerase chain reaction (PCR) was used to amplify CSN3-specific regions, and amplified products were run using 2% agarose gel electrophoresis (AGE). Various restriction endonucleases (RE) were utilized in respect to their protocols to investigate polymorphisms in amplified products. Following the RE digestion, mixtures were run in 2.5% AGE. It was observed that only the BB genotype was obtained in terms of Hinf I enzyme; however, the E allele (Mae III) was not obtained for the CSN3 gene. For the Hind III enzyme, only the BB genotype was obtained from exon 4 of the CSN3 gene, while both AB (27.41%) and BB (72.59%) genotypes were determined from exon 4-intron 5 of the CSN3 gene. No statistical difference was detected for genotypes of the restriction enzymes used in Anatolian water buffaloes (P > 0.05). In conclusion, the Hinf I/BB genotype, associated with higher milk protein amount and milk fat, was observed in Anatolian water buffaloes.Öğe Comparisons of some physiological and stress behavioral parameters of Kangal shepherd dogs with and without ankyloglossia in different environmental temperatures(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2019) Kockaya, Mustafa; Ozsensoy, Yusuf; Insal, BurcuThe aim of this study was to compare Kangal shepherd dogs with and without ankyloglossia in terms of various physiological and behavioral changes. A total of 24 dogs were used, 11 of which have had ankyloglossia and 13 of which did not have ankyloglossia. Heart rates, respiratory rates, and body temperatures of the dogs were measured at three different environmental temperatures (18, 23, and 28 degrees C), resting state, and different exercise durations (5, 10, and 15 min). In addition, resting state and some behavioral parameters were recorded with a video camera. Mann-Whitney U, Kruskal-Wallis, and Wilcoxon tests were used to analyze the data. While no statistical difference was detected in the two groups in terms of all parameters during the resting state at all temperatures, the difference between the resting state and exercising was statistically significant (P < 0.05, P < 0.01). Except for 15 min of exercise for the respiration rates, statistical differences were detected in both groups in parameters at both different temperatures and different exercise durations at each temperature. While some of the stress behavior parameters were not detected at any of the temperatures in normal dogs, the number of the dogs showing other stress behavioral parameters increased in both groups as the temperature increased, but the number was higher in the dogs with ankyloglossia. This study is the first study on physiological and stress behavioral parameters in Kangal shepherd dogs with ankyloglossia.Öğe Genetic diversity and population structure of wheat in India and Turkey(OXFORD UNIV PRESS, 2015) Khan, Mohd Kamran; Pandey, Anamika; Thomas, George; Akkaya, Mahinur S.; Kayis, Seyit Ali; Ozsensoy, Yusuf; Hamurcu, Mehmet; Gezgin, Sait; Topal, Ali; Hakki, Erdogan E.Genetic diversity among plant species offers prospects for improving the plant characteristics. Its assessment is necessary to help tackle the threats of environmental fluctuations and for the effective exploitation of genetic resources in breeding programmes. Although wheat is one of the most thoroughly studied crops in terms of genetic polymorphism studies, phylogenetic affinities of Indian and Turkish Triticum species have not been assessed to date. In this study, genetic association of 95 tetraploid and hexaploid wheat genotypes originating from India and Turkey was determined for the first time. Combined analysis of random amplified polymorphic DNA and inter-simple sequence repeat markers disclosed 177 polymorphic bands, and both the dendrogram and two-dimensional scatterplot showed similar groupings of the wheat genotypes. Turkish hexaploid varieties were basically divided into two clusters, one group showed its close association with Indian hexaploid varieties and the other with Indian tetraploid varieties. Analysis of molecular variance revealed high (77 %) genetic variation within Indian and Turkish populations. Population structure analysis elucidated distinct clustering of wheat genotypes on the basis of both geographical origin and ploidy. The results revealed in this study will support worldwide wheat breeding programmes and assist in achieving the target of sustainable wheat production.Öğe Genetic Diversity of Eight Domestic Goat Populations Raised in Turkey(HINDAWI LTD, 2016) Bulut, Zafer; Kurar, Ercan; Ozsensoy, Yusuf; Altunok, Vahdettin; Nizamlioglu, MehmetThe objective of this study was to determine the intra- and intergenetic diversities of eight different goat populations in Turkey including Hair, Angora, Kilis, Yayladag, Shami, Honamli, Saanen, and Alpine. A total of 244 DNA samples were genotyped using 11 microsatellites loci. The genetic differentiation between breeds was considerable as a result of the statistically significant (P < 0.001) pairwise F-ST values of each pair of breeds. Exceptionally, F-ST values calculated for Honamli and Hair breeds were statistically nonsignificant (P > 0.05). Heterozygosity values ranged between 0.62 and 0.73. According to the structure and assignment test, Angora and Yayladag goats were assigned to the breed they belong to, while other breeds were assigned to two or more different groups. Because this study for the first time presented genetic data on the Yayladag goat, results of structure analysis and assigned test suggest that further analyses are needed using additional and different molecular markers.Öğe Investigation of Genetic Structures of Coloured Horses by mtDNA D-loop Sequence Analysis in Turkey(Kafkas Univ, Veteriner Fakultesi Dergisi, 2019) Koseman, Abdurrahman; Ozsensoy, Yusuf; Erdogan, Metin; Yarali, Cevdet; Toprak, Burhan; Zengin, Kemal; seker, IbrahimThe aim of this study was to determine the genetic structure of Coloured horses in Turkey by analysis of the D-loop sequence of mitochondrial DNA (mtDNA). A total of 28 Coloured horses were examined. DNA was extracted from blood samples using DNA isolation kit; 519 bp long mtDNA D-loop region was amplified by PCR and sequenced by capillary electrophoresis system. Population parameters and phylogenetic trees were drawn by using MEGA4 software package. It was also compared with the DNA sequences of horse populations from different countries. In this study, 42 different polymorphism regions and 10 different haplogroups were detected. Additionaly, Tajima D value was found to be -0.31 and population expansion was determined. It was determined that the base differences among the horses ranged between 0.000 and 0.032. It was detected that the horses formed different clusters from each other and they were intertwined with the populations of different countries. Moreover, it was also observed that some horses formed their own populations at different points from the other countries' horses. As a result, it was observed that the genetic structures of horses which used in population were different from each other and they originated from different mothers. According to the results obtained, it is considered that Coloured horses can be a native horse breed of Turkey.Öğe Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method(Tubitak Scientific & Technological Research Council Turkey, 2019) Ozsensoy, Yusuf; Kara, HakiThe Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific pruners in PCR. PCR products were digested by Alu I (GH, n: 167) and Alu I (GHR, n: 192) restriction enzymes in order to determine polymorphisms. Digested PCR products were separated in 2.5%-3% agarose gel electrophoresis to determine allelic polymorphisms. As a result, the LL (78.44%) and LV (21.56%) genotypes and L (0.89) and V (0.11) alleles for the GH gene and the AA (7.81%) and AG (92.19%) genotypes and A (0.54) and G (0.46) alleles for the GHR gene were obtained. Gene polymorphisms were not detected (P > 0.05) on the Gil gene, but a significant difference was found for the GHR gene (P < 0.001). Therefore, it can be said that Anatolian water buffaloes have increased meat yield due to the presence of genotypes in gene regions. This was the first investigation of enzyme polymorphisms of the GHR gene in Anatolian water buffaloes.Öğe Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method(REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI, 2018) Ozsensoy, YusufThe objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by separation of fragments obtained from digested PCR products in 3% agarose gel electrophoresis. AA genotype (HaeIII) and BB genotype (RsaI) of exon 1 and only AA genotype (RsaI) of exon 3 were obtained. No polymorphisms were determined in Anatolian water buffalo breed and all loci were found as monomorphic. It can be stated that Anatolian water buffalo has a higher milk and milk fat yields since BB genotype was obtained.Öğe MnSOD, CAT and GPx-3 genetic polymorphisms in coronary artery disease(SPRINGER, 2019) Dogan, Ayse; Ozsensoy, Yusuf; Turker, Fevzi SarperIn this study, we aimed to determine the gene polymorphisms of antioxidant enzymes that determine or affect antioxidant activity in the occurrence of the disease and/or complications during and after the surgery in patients who were decided to undergo coronary artery bypass surgery due to coronary artery disease. Blood samples taken before operation in 26 coronary artery patients who were decided to be operated according to the international procedure and the phenol/chloroform method was used to isolate DNA. DNA samples were amplified by using polymerase chain reaction (PCR) method with specific primers for MnSOD, CAT, GPx-3 antioxidant gene regions. As a result of the increasing process, the PCR products for the purpose of determining gene polymorphism, NGOMIV SMA f and BSA I restriction enzymes were used for MNSOD, CAT and GPx-3 gene region, respectively. Allele frequencies were determined and compared by Chi square test. VV (46.15%) and VA (53.85%) genotype for MnSOD region, i TT (22.22%), TC (16.67%) and CC (61.11%) genotype for CAT region, and CC (12.50%), TC (25%) and TT (62.50%) genotypes for GPx-3 region were obtained. While there was no statistically significant significance in terms of genotypes obtained in MnSOD and GPx-3 gene regions (P>0.05), a significant difference was found in the CAT gene region in terms of genotypes (P<0.01). Although oxidative stress is important in relation to cardiovascular diseases and postoperative complications, virtually no study of antioxidant enzymes in gene polymorphism are included in the literature. Work is lacking in relation to the subject.Öğe Morphological and morphometric traits of Turkiye's Aseel chicken(Ankara Univ, 2025) Kocakaya, Afsin; Bulut, Fatma Tulin Ozbaser; Ozkul, Banu Yuceer; Ozsensoy, Yusuf; Ozbeyaz, CeyhanThis research studies the morphology and morphometry traits of Aseel chickens raised in various regions of Turkiye. We used Turkish Aseel genotypes to assess the live weight and physical features of 60 female and 58 male subjects of varying ages. We measured brood length, breadth, head and beak width, neck and body length, chest width, depth, and circumference, thigh diameter, length, and depth, and leg length. Males' average live weight, chest circumference, thigh length, and comb length, which affect game efficacy, are 3.23 kg, 37.88 cm, 20.69 cm, and 40.20 mm, respectively. Although there was a statistically significant difference between the gender groups for all evaluated characteristics, there was only a statistically significant difference between the age groups for head width and chest circumference, shank depth, diameter, and length, and live weight (P<0.05). The data showed differences among different areas, particularly about the measurements of the comb, prompting researchers to propose a hypothesis suggesting a potential correlation between these changes and regional temperature disparities. We evaluated Turkish Aseels for feather, comb, eye color, markings, and comb type. Medium-weight breeds with hair, earlobes, beards, and spurs Weight and characteristics determine the Turkish Aseel breed. Comb structures were shorter, and their live weight was lower than that of other game roosters or Aseel kinds. Based on their morphology, domestic Aseel males may have a distinct genotype and subtype. Evaluating morphometric characteristics is an important aspect of the research. However, conducting additional comparative research is crucial. Genotyping studies with larger numbers are necessary for greater accuracy.Öğe Parentage verification and genetic diversity of the Arabian and Thoroughbred horse populations in Türkiye using microsatellite analysis(Gesellschaft Schweizer Tierarztinnen & Tierarzte, 2023) Yarali, Cevdet; Ozsensoy, Yusuf; Koseman, Abdurrahman; Seker, Ibrahim; Toprak, Burhan; Zengin, KemalThis study aimed to determine the effectiveness of parentage verification in Arabian and Thoroughbred horses in Turki-ye using microsatellite markers. A total of 813 Arabian and 959 Thoroughbred horses were genotyped using a total of 17 microsatellite markers. The mean effective number of alleles was 3,34 and the mean number of alleles was 7,41 in Arabian horses. It was calculated that the mean He and Ho values in Arabian horses were 0,677 and 0,680, respective-ly. The mean effective number of alleles was 3,55 and the mean number of alleles was 6,59 in Thoroughbred horses. It was calculated that the mean Ho and He values in Thor-oughbred horses were 0,697 and 0,684, respectively. When the studied Arabian and Thoroughbred horse populations are considered as a single population, the mean FIT, FST and FIS values were found to be 0,063, 0,074 and, - 0,011, re-spectively. Also, 4 loci in Arabian horses and 3 loci in Thor-oughbred horses significantly deviated from HWE. The mean PIC value was 0,63 in Arabian horses and 0,64 in Thoroughbred horses. As a result; the microsatellites includ-ing the most informative 15 and 9 loci had a total value of > 0,9999 (11 and 7 loci > 0,999) in each population for PE - 1 and PE - 3, respectively. It has been concluded that parentage verification and genetic identification can be made successfully in the Arabian and Thoroughbred horse populations by using the microsatellite markers panel.Öğe Phylogenetic and bottleneck analysis of the Turkish Arabian and Thoroughbred horse populations(Univ Zulia, Facultad Ciencias Veterinarias, 2023) Yarali, Cevdet; Koseman, Abdurrahman; Ozsensoy, Yusuf; Seker, Ibrahim; Toprak, Burhan; Zengin, KemalThis study aimed to determine the phylogenetic and bottleneck analyses of Turkish Thoroughbred and Arabian horse populations. In the study, genotyping was performed using a total of 17 microsatellite markers in the samples taken from 959 Thoroughbred and 813 Arabian horses. The average effective allele number in Arabian horses was 3.338 and the average number of alleles was 7.412 in Thorougbred horses. Genetic distance and genetic identity between Thoroughbred and Arabian breeds was 0.411 and 0.663, respectively. Also genetic identity in each Arabian and Thoroughbred horse populations was 1.000. The FCA table showed that the two breeds were completely separated from each other and were compatible. In conclusion; the bottlenecks of Thoroughbred and Arabian horse populations were in a normal L distribution and these horse breeds do not appear to have succumbed to introgression. Therefore, they are not yet at risk of extinction any time soon.Öğe Phylogenetic relationships of native Turkish cattle breeds using microsatellite markers(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2019) Ozsensoy, Yusuf; Kurar, Ercan; Dogan, Muge; Bulut, Zafer; Nizamlioglu, Mehmet; Altunok, Vahdettin; Isik, Ayse; Camlidag, AysunA total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, respectively. TGLA122 was the most polymorphic locus; however, only 7 different alleles were observed for INRA005. A total of 40 different private alleles were determined. The general F-IS values were between 0.034 and 0.123. Due to the close location to the domestication center, higher genetic diversities were observed. The observed genetic diversities and the results of the phylogenetic analyses were in agreement with evolutionary history and the geographical origins of Turkish native cattle breeds.
