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Öğe Balneoterapinin fibromiyalji hastalarında ağrı ve yaşam kalitesine etkisi(Hatay Mustafa Kemal University, 2018) Karadağ, Ahmet; Parlak, Mesut; Canbaş, MuhammetAim: The aim of this study was to investigate effect of balneotherapy (BT) on pain and quality of life in patients with fibromyalgia (FMS). Material and Methods: The study included 98 women without known metabolic or psychiatric disorder and history of new medication within prior month who were diagnosed as primary fibromyalgia according to 2010 American College of Rheumatology (ACR) criteria and accepted to participate to the study. The sociodemographic characteristics were recorded for all patients. All patients received 21 consecutive sessions of balneotherapy (20 minutes per day). The patients were assessed by Fibromyalgia Impact Questionnaire (FIQ) and Visual Analog Scale (VAS) before and after therapy. Results: The mean age of the all patients was 53.04 ± 10.3 years, the mean body mass index was 29.06 ± 5.9 kg/m2, and the mean disease duration was 54.91 ± 59.5 months. In patients with FMS, there were significant differences between VAS and FIQ scores assessed before and after BT (p=0.01). Also, the extent of decrease in FIQ score was greater in non-smokers than smoker FM patients (p<0.05). Conclusion: In patients with FM, there was a significant difference in VAS scores following BT. Moreover, there was also a significant difference in FIQ scores. However, the improvement in quality of life after BT was greater in non-smoker FM patients when compared to smoker FM patient.Öğe Effects of Metformin on TNF- Release in Lipopolysaccharide-Induced Monocytes in Rats(Sivas Cumhuriyet Üniversitesi, 2024) Gedikli, Emre; Parlak, Mesut; Soydan, Ahmet SerdarObjective: Inflammation which is a response of immune system was demonstrated in many disorders such as atherosclerosis, hypertension, diabetes, cancer and rheumatoid arthritis. Metformin, an oral antidiabetic drug, has anti-inflammatory effect apart from blood glucose regulatory effect. However, the mechanism of its anti-inflammatory effect is not clearly understood. In this study, the effect of metformin on the release of cytokines (TNF-α and IL-6) from LPS stimulated rat mononuclear blood cells was investigated. Methods: Blood samples (5ml) were taken from healthy, male, 8-12 weeks old rats (n=5, 200-250g) through cardiac puncture under general anesthesia into sterile EDTA containing tubes. Monocytes were separated by centrifugation and were resuspended in RPMI 1640 media (3.3±0.2x105 /ml). Cells were then incubated with metformin (2.5 μM, 25 μM, 250 μM) for 2,5 hours followed by addition of LPS (100 ng/ml, 1μg/ml) for further 5 hours. After centrifugation, the supernatant was taken and TNF-α level was measured by ELISA. Results: There was no statistically significant change in the amounts of TNF-α in the LPS + metformin groups compared to the 100 ng/ml LPS group (p>0.05). In LPS+metformin groups, compared to 1 μg/ml LPS, 2.5 μM and 250 μM metformin significantly increased TNF-α levels (p0.05). The amount of IL-6 was not within measurable range in this study. Conclusion: In summary, metformin increased the amount of released TNF-α rather than decreased in our study.Öğe Evaluation of effects of T and N type calcium channel blockers on the electroencephalogram recordings in Wistar Albino Glaxo/Rij rats, an absence epilepsy model(MEDKNOW PUBLICATIONS & MEDIA PVT LTD, 2015) Durmus, Nedim; Gulturk, Sefa; Kaya, Tijen; Demir, Tuncer; Parlak, Mesut; Altun, AhmetObjectives: It is suggested that excessive calcium entry into neurons is the main triggering event in the initiation of epileptic discharges. We aimed to investigate the role of T and N type calcium channels in absence epilepsy experimental model. Materials and Methods: Wistar Albino Glaxo/Rij (WAG/Rij) rats (12-16 weeks old) were randomly allocated into four groups; sham, mibefradil (T type calcium channel blocker), w-Conotoxin MVIIA (N type calcium channel blocker), and mibefradil w-Conotoxin MVIIA. Beta, alpha, theta, and delta wave ratios of EEG recordings and frequency and duration of spike wave discharges (SWDs) were analyzed and compared between groups. Results: Beta and delta recording ratios in 1 M/5 l mibefradil group was significantly different from basal and other dose-injected groups. Beta, alpha, and theta recordings in 0.2 M/5 l w-Conotoxin MVIIA group was significantly different from basal and other dose-injected groups. In w-Conotoxin MVIIA after mibefradil group, beta, alpha, and theta recording ratios were significantly different from basal and mibefradil group. Mibefradil and w-Conotoxin MVIIA significantly decreased the frequency and duration of SWDs. The decrease of frequency and duration of SWDs in mibefradil group was significantly different from w-Conotoxin MVIIA group. The frequency and duration of SWDs significantly decreased in w-Conotoxin MVIIA after mibefradil group compared with basal, mibefradil, and w-Conotoxin MVIIA groups. Conclusions: We concluded that both T and L type calcium channels play activator roles in SWDs and have positive effects on frequency and duration of these discharges. These results are related with their central effects more than peripheral effects.Öğe In vitro evaluation of nebivolol effects on nonadrenergic noncholinergic responses in rabbit corpus cavernosum(WILEY, 2018) Aydin, Kaya; Gokcen, Kaan; Yildirim, Sahin; Bagcivan, Ihsan; Parlak, Mesut; Gokce, GoekhanThe purpose of this study was to compare the effects of nebivolol on nonadrenergic noncholinergic (NANC) relaxation functions that are mediated by electric field stimulation (EFS) in rabbit corpus cavernosum smooth muscle by comparison with other beta-adrenergic receptor blockers and show the level on which its effects through nitric oxide take place. After the effects of nebivolol on the isolated corpus cavernosum tissues that were contracted through the alpha-adrenergic pathway and application of L-NAME' (N-G-nitro-L-arginine methyl ester) which is a competitive inhibitor of nitric oxide synthase (NOS), the changes that occurred were recorded. Following the effect on the tissue that was contracted with phenylephrine in the presence of atropine and guanethidine that was created by EFS, nebivolol and other beta-blockers were added and the changes were recorded. After receiving relaxation responses with EFS-mediated NANC, no difference was observed between the relaxation responses due to addition of nebivolol and other beta-adrenergic blockers (p > 0.05). The finding that nebivolol which has a NO-mediated relaxation effect did not have an effect on EFS-mediated NANC relaxation but created relaxation on the tissue that was contracted by phenylephrine and the effect was reversed by L-NAME, shows that its effects are on a postsynaptic level.Öğe Investigation of mechanical strength of teicoplanin and ciprofloxacin impregnated bone cement on Day 1 and Day 15(TURKISH ASSOC ORTHOPAEDICS TRAUMATOLOGY, 2014) Golge, Umut Hatay; Oztemur, Zekeriya; Parlak, Mesut; Tezeren, Gunduz; Ozturk, Hayati; Bulut, Okay…Öğe Investigation of the role of the NO-cGMP pathway on YC-1 and DEA/NO effects on thoracic aorta smooth muscle responses in a rat preeclampsia model(CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS, 2013) Turgut, Nergiz Hacer; Temiz, Tijen Kaya; Turgut, Bulent; Karadas, Baris; Parlak, Mesut; Bagcivan, IhsanThe present study was designed to investigate the effects of YC-1, a nitric oxide (NO)-independent soluble guanylate cyclase (sGC) activator, and DEA/NO, a NO donor, on smooth muscle responses in the preeclampsia model with suramin-treated rats and on the levels of cyclic guanosine monophosphate (cGMP) of thoracic aorta rings isolated from term-pregnant rats. Rats of 2 groups, control group and suramin group, were given intraperitoneal injection of saline or suramin, respectively. Suramin injection caused increased blood pressure, protein in urine, and fetal growth retardation. Thoracic aorta rings were exposed to contractile and relaxant agents. KCl contraction and papaverine relaxation responses were similar. Relaxation responses of YC-1 and DEA/NO decreased in suramin group. In both groups in the presence of ODQ, a sGC inhibitor, the relaxation responses of YC-1 and DEA/NO decreased. The cGMP content was determined by radioimmunoassay technique. The content of cGMP in the suramin group decreased. In the presence of YC-1 and DEA/NO in both groups, cGMP content increased, but in ODQ-added groups, there was a significant decrease. We conclude that in preeclampsia, the decrease of relaxation responses and the decrease of cGMP content could be due to the reduction in stimulation of sGC and the decrease in cGMP levels.Öğe Isolation and Antibiotic Resistance Rates of Mycobacterium tuberculosis complex in Mycolor TK® System(Doc Design Informatics Co Ltd, 2024) Yildirim, Dilara; Aldemir, Ozlem; Yanik-Yalcin, Tugba; Parlak, MesutObjective: Tuberculosis (TB) is a public health problem. This study aimed to determine the growth rates and drug susceptibility levels of patients with Mycobacterium tuberculosis complex (MTC) growth in cultures obtained and to compare the results with the growth rates and drug susceptibility levels found in our country and other countries. It also aimed to evaluate the results of supplementing classical methods such as Lowenstein-Jensen (LJ) with liquid TK MEDIUM (R) and to determine the relationship between the growth rates obtained with both methods. Materials and Methods: The study included patients who were Erlich Ziehl Neelsen (EZN) positive or negative and whose samples showed MTC growth in culture and susceptibility to the main drugs used in the treatment of TB, including streptomycin (SM), isoniazid (INH), rifampicin (RIF) and ethambutol (EMB). Patient samples were inoculated simultaneously into liquid TK (R) MEDIUM based on color change and LJ solid medium. TB identification and antimicrobial susceptibility testing were performed using the TK ANTI TB and PNB KIT (R) for the Mycolor TK (R) automated mycobacterial culture system. Results: The study found resistance to dual drugs at 5.4%, INH+RIF at 1.35%, INH at 6.8%, RIF at 14.8%, EMB at 17.6%, and SM at 1.35%. Out of 69 samples cultured in LJ medium, three failed to grow in liquid TK (R) MEDIUM, and two of 71 samples cultured in liquid TK (R) MEDIUM gave negative results in solid LJ medium. Conclusion: We found that the drug resistance rates we obtained in our study were similar to the results of the Republic of T & uuml;rkiye Ministry of Health and other studies conducted in our country. In a joint study conducted with liquid and solid media, no difference was found in the detection of TB bacilli. Mycolor TK (R) automated system can enable rapid mycobacterial culture in laboratories with limited resources.Öğe Lycopene induces antiproliferative effects through apoptosis, autophagy, and oxidative DNA damage in the HeLa cells(Taylor & Francis Ltd, 2024) Parlak, Mesut; Joha, Ziad; Yulak, Fatih; Mendil, Ali Sefa; Tastemur, YasarBackground: This study explores the role of apoptosis, autophagy, and oxidative DNA damage in influencing the cytotoxic impact of lycopene on HeLa cells. Material and methods: Cell viability following exposure to varying lycopene concentrations was determined using an XTT assay. ELISA measured key cell death proteins (Bax, BCL-2, etc.), while immunofluorescence staining visualized LC3 beta (autophagy) and 8-oxo-dG (DNA damage). Results: Lycopene significantly killed HeLa cells in a dose-dependent way (IC50 = 10 mu M). Subsequent examinations conducted with the IC50 dose of lycopene demonstrated a notable elevation in the expression levels of apoptotic proteins, such as cleaved caspase 3, cleaved PARP, and Bax (p < 0.001). Additionally, treatment with this substance led to an increase in the levels of 8-oxo-dG (p < 0.001), a widely acknowledged biomarker indicative of oxidative DNA damage. Furthermore, a significant rise (p < 0.05) in LC3 beta protein levels, a well-established indicator of autophagy activation, was noted. Conclusion: This study suggests lycopene's potential to fight cervical cancer by triggering programmed cell death (apoptosis) and cellular self-digestion (autophagy). These findings highlight lycopene as a promising candidate for future cervical cancer treatments.Öğe Simvastatin ve mevastatin'in anjiogenez inhibisyonu üzerine etkilerinin koryoallantoik membran modelinde araştırılması(2012) Beşikçi, Zeliha Ödemiş; Kaya, Tijen Temiz; Balcı, Ezgi; Parlak, Mesut; Altun, AhmetAmaç. Bu çalışmanın amacı klinik tedavide sık olarak kullanılan statinlerdenmevastatinin anjiogenez inhibisyonu üzerine etkilerini döllenmiş tavuk yumurtaları üzerinde,koryoallantoik membran modelini (CAM modeli) kullanarak kıyaslamaktır. Yöntem. ÇalışmadaRoss 308 cinsi döllenmiş tavuk yumurtaları kullanıldı. Döllenmiş tavuk yumurtaları 37,5°C?de%80 rölatif nemli ortamda horizontal pozisyonda inkübe edildi. Kuluçkanın beşinci günündeyumurtanın künt tarafından enjektör yardımıyla 5 mL albumin alındı ve yumurtanın diğer ucundan2-3 cm çapında kabuk kesilerek çıkarıldı. Kabuktaki bu açıklık laboratuvar filmi ile kapatıldı vekoryoallantoik membran modeli yaklaşık 2 cm çapa ulaşana kadar 72 saat daha inkübe edildi. Herbir yumurtaya koryoallantoik membran üzerine etken madde içeren bir pellet yerleştirildi. İlaçuygulamasından (bevacizumab, simvastatin ve mevastatin; 10-4M, 10-5M ve 10-6Mkonsantrasyon) sonra yumurtalar 24 saat daha inkübe edildi. Stereoskopik mikroskop altındaBürgermeister ve arkadaşlarının skorlama sistemi kullanılarak pellet uygulama bölgesindeki damaryapısı değerlendirildi. Bulgular. Çalışmamızda negatif kontrol olarak ilaç içermeyen agar disklerkullanıldı. Negatif kontrol yumurtalarının hiç birinde anjiogenez engellenmedi ve anti-anjiogeniketki puanı 0 olarak bulundu. Pozitif kontrol olarak antianjiogenik etkinliği kanıtlanmışbevacizumab kullanıldı. Bevacizumab?ın 10-4M,10-5M ve 10-6M konsantrasyonlarındaantianjiogenetik skor değerleri sırasıyla 1,58 , 1,55 ve 1,00 olarak bulundu. Simvastatinin vemevastatinin 10-4M, 10-5M ve 10-6M konsantrasyonları için ortalama antianjiyogenik skordeğerleri sırası ile 0,93 , 0,66 , 0,53 ve 1,0, 0,80 ve 0,66 olarak bulundu. Hem simvastatinin hemde mevastatinin her üç konsantrasyonunun da anti anjiogenik etkiye sahip olduğu saptandı. Bu ikiilacın 10-4M konsantrasyonlarının meydana getirmiş olduğu anti anjiogenik etki gücü arasındaanlamlı bir fark bulunamadı. Ancak azalan konsantrasyonlar birbirleri ile karşılaştırıldığındamevastatinin 10-5M ve 10-6 M konsantrasyonlarda simvastatine göre daha anlamlı bir antianjiogenik etki meydana getirdiği saptandı. Sonuç. Sonuç olarak simvastatin ve mevastatin CAMmodelinde antiantiogenik etkinlik gösteren statinlerdir. Bu iki statin lipid düşürücü etkileriyanında, anjiogenez inhibisyonu yaparak; plak içi antianjiogenik etkiyle, plak rüptürü ve bunabağlı ciddi komplikasyonlardan da korunma sağlayabilir.Öğe Tirozin kinaz inhibitörü olan Motesanibin tek başına ve nitrik oksit sentaz inhibitörü L-NIO ve SMT ile kombinasyonlarının HT29 kolorektal kanser hücreleri üzerine etkileri(Cumhuriyet Üniversitesi, 2013) Parlak, Mesut; Yıldırım, Mustafa KemalÖZET Kanser günümüz toplumunun en büyük problemlerinden birini teşkil etmektedir. Birçok türü için tedavisi mümkün olmayan ölümcül bir hastalık olması yanında kanser tedavisi oldukça zor ve pahalı bir hastalıktır. Günümüzde kullanılan kanser ilaçlarının büyük çoğunluğu hücre bölünmesi üzerine etkili olup esas etkinliklerini hızlı bölünen kanser hücreleri yanında vücudun çok bölünen normal hücreleri üzerinde de gösterirler. Bu yüzden yeni tedavi yaklaşımları anjiojenez inhibisyonu ve apoptozis üzerine yoğunlaşmaktadır. Bu çalışmada Motesanib, L-NIO ve SMT'nin tek başlarına ve kombine olarak kolorektal kanser üzerine etkilerini araştırmayı amaçladık. Motesanib, L-NIO ve SMT'nin kolorektal hücre proliferasyonu üzerine olan etkilerini belirlemek için gerçek zamanlı hücre analiz sistemi (xCELLigence sistem) kullanıldı. Ajanların anjiojenez üzerindeki etkileri koryoallantoyik mebran modeli, apoptozis ürerinde olan etkileri ise Annexin V boyama yöntemi ile belirlendi. Bu çalışmada, tek başına Motesanib ve tek başına L-NIO'nun HT29 kolorektal kanser hücrelerinin çoğalmasını ve anjiojenezini güçlü bir şekilde inhibe ettiğini ve güçlü bir apoptozis oluşturduğunu bulduk. Fakat SMT'nin bütün dozları etkisizdi. Motesanib'in L-NIO ve SMT ile kombinasyonları HT29 kolorektal kanser hücrelerinde tek başlarına yaptıkları etkilerden daha fazla etki oluşturdu. Bu çalışma göstermektedir ki, tirozin kinaz inhibitörleri ile NOS inhibitörlerinin kombinasyonları düşük konsantrasyonlarda tirozin kinaz ve NOS inhibitörü kullanılmasına izin verebilir, tirozin kinaz inhibitörlerine gelişen direnci önleyebilir ve yan etkilerini azaltabilir. Bu ilaçların etki mekanizmasını tespit etmek için daha ileri çalışmaların gerekli olduğuna inanıyoruz.
