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Öğe 3-metilkolantren ve bütil hidroksitoluen ile indüklenmiş rat akciğer dokusunda ki-ras ekson 2 gen mutasyon analizi(Cumhuriyet Üniversitesi, 2003) Polat, Fikriye; Özdemir, Öztürk68 6. ÖZET Bu çalışmada, bir grup rata (Rattus norvegicus wistar albino) kanser indükleyici ajanlardan 3-Metilkolantren (MCA) 40 mg/kg dozda haftada bir kez, altı hafta boyunca ; diğer grup rata bir antioksidan olan Bütil hidroksitoluen (BHT) 200 mg/kg dozda yine haftada bir kez olmak üzere altı hafta boyunca ve bir başka gruba ise tek doz 3-Metilkolantren (40 mg/kg) ardından haftada bir kez altı hafta boyunca Bütil hidroksitoluen (200 mg/kg) intraperitonal olarak uygulandı. 26 hafta sonra ratlar servikal dislokasyon yolu ile öldürülerek akciğer dokuları alındı. Daha sonra ratlarm akciğerleri sintigrafik, radyolojik ve morfolojik olarak değerlendirildi. Dokulara DNA izolasyonu yapıldıktan sonra Ki- ras ekson 2 geni Polimeraz zincir reaksiyonu- tek zincir konformasyon polimorfizmi- DNA sekans yöntemiyle incelendi. Ancak yapılan incelemeler sonucunda Ki- ras ekson 2 geninde herhangi bir mutasyon tespit edilemedi.Öğe Analysis of Ki-ras exon 2 gene mutations in 3-methylcholanthrene and butylated hydroxytoluene-induced rat lung tissues(2008) Polat, Fikriye; Özdemir, Öztürk; Elagöz, Şahende3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies. Butylated hydroxytoluene (BHT) has been widely used for many years as an antioxidant to preserve and stabilize the freshness, nutritional value, flavor, and color of foods. The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2. Rats were killed by cervical dislocation 26 weeks after the last BHT injection and lung tissues were surgically removed. Lung tissues of the control and experiment rat groups were examined for point mutations in the exon 2 of the Ki-ras by PCR based SSCP analysis. No point mutation was observed in the Ki-ras gene exon 2. © TÜBİTAK.Öğe The Association of MYNN and TERC Gene Polymorphisms and Bladder Cancer in a Turkish Population(UROL & NEPHROL RES CTR-UNRC, 2019) Polat, Fikriye; Yilmaz, Meral; Diler, Songul BudakPurpose: Researchers reported that, MYNN rs10936599 polymorphism is in strong or moderate linkage disequilibrium with SNPs within the 3q26.2 chromosomal regions that also include the TERC gene. In addition, it has been reported that MYNN rs10936599 had a strong cumulative association with bladder cancer risk, and TERC gene suppresses cell growth in bladder cancer cell lines. Therefore, we aimed to determine whether polymorphisms of MYNN rs10936599 and TERC rs2293607 play any roles for bladder cancer in the Turkish population in this study. Materials and Methods: In this case-control study, 70 patients and 150 controls were investigated. Genotyping analysis was performed by polymerase chain reaction, restriction fragment length polymorphism and DNA sequencing techniques. Results: Genotype distribution between study groups for MYNN rs10936599 SNP was significantly different (P = .001); although there was no difference in genotype distribution for TERC rs2293607 SNP. In addition, patients with CT genotype and CT+TT genotype combination of MYNN SNP have a decreased risk for bladder cancer. Two times increased risk ratio on development of bladder cancer was obtained for CC genotype of the SNP (P = .001). Besides, it was found that genotype combination of GG+AG/CC versus AA/CC genotypes (TERC/MYNN) showed stronger correlation. We observed that statistically significant relationship between the C-G haplotypes of two polymorphisms and bladder cancer risk (P = .0001). Conclusion: At the end of the study, we suggested that there may exist an association between a combination of MYNN rs10936599 and TERC rs2293607 polymorphisms and development of bladder cancer in Turkish population.Öğe Combined Germline Variations of Thrombophilic Genes Promote Genesis of Lung Cancer(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2013) Ozen, Filiz; Polat, Fikriye; Arslan, Sulhattin; Ozdemir, OzturkBackground: A large variety of familiar and non-familiar lung carcinomas (LC) are caused by long term exposure to chemical carcinogens that are present in tobacco smoke. We aimed to investigate the prevalence of 5 thrombophilic germ-line mutations in patients with lung carcinomas. Materials and Methods: A total of 52 LC patients and 212 healthy controls from same population were analyzed for FV Leiden, factor V H1299R (R2), PAI-1, MTHFR C677T, MTHFR A1298C, ACE I/D, and Apo E genes and compared. Results: Overall, heterozygous and/or homozygous point mutations in FV Leiden Apo E2, PAI-1 and MTHFR C677T genes were associated with LC in the current cohort. There was no meaningful association between LC and ACE I/D gene markers. Conclusions: The current results showed that LC is related to combined thrombophilic gene mutations and individuals with homozygosity of 4G in PAI-1 and MTHFR C677T genes and heterozygosity of FV Leiden, Apo E4 genes have a germ-line risk for LC tumorigenesis.Öğe The effect of 3-methylcholanthrene and butylated hydroxytoluene on glycogen levels of liver, muscle, testis, and tumor tissues of rats(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2013) Polat, Fikriye; Dere, Egemen; Gul, Eylem; Yelkuvan, Izzet; Ozdemir, Ozturk; Bingol, GunselThis study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group (n = 9) and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene (n = 9) or butylated hydroxytoluene (n = 11) or a combination of these agents (n = 14). Rats were intraperitoneally injected with a 200 mg kg(-1) dose of butylated hydroxytoluene and a 40 mg kg-1 dose of 3-methylcholanthrene. At the end of the 26-week experimental period, tissues of rats killed via cervical dislocation were placed in 10% trichloroacetic acid for glycogen determination. Our results showed that the administration of 3-methylcholanthrene, butylated hydroxytoluene, and 3-methylcholanthrene + butylated hydroxytoluene caused statistically significant changes in the glycogen levels of liver, muscle, and testis tissues, and glycogen was stored in tumor tissue.Öğe Paraquat’ın farelerin akciğerlerindeki adenozin deaminaz aktivitesi üzerine etkilerinin araştırılması(Cumhuriyet Üniversitesi, 1998) Polat, Fikriye; Çolak, Ahmet29 6. ÖZET Bu çalışmada, yabancı otlarla mücadelede bir herbisit olarak kullanılan Paraquat 'm LD30 (20 mg.kg-10) dozu Mus musculus- Swiss albino farelere intraperitona! yoldan uygulanarak 2, 4, 8, 16, 32, 64 ve 72 saat sonra akciğer Adenozin deaminaz aktivitesi üzerine etkisi araştırılmıştır. Çalışmada, Paraquat akciğerlerde Adenozin deaminaz aktivitesinde başlangıçta önemli bir inhibisyon oluştururken 16. saatten sonra bu inhibisyon yerini aktivitasyona bırakmış, 64 ve72. saatlerde istatistiksel olarak önemli bir artış bulunmuştur. 7. SUMMARY INVESTIGATION OF THE EFFECTS OF PARAQUAT ON ADENOSINE DEAMINASE ACTIVITY IN THE LUNGS OF MICE (Mus musculus - Swiss albino) In this study, the effects of a LD30 (20 mg.kg"1) dose of Paraquat that is widely using in the struggle of the wild plants as a herbiside has been investigated on Adenosine deaminase activity in the lung of mice after 2, 4, 8, 16, 32, 64 and 72 hours it was applied intraperitoneaily to Mus musculus- swiss albino. It has been found that the agent caused significant inhibition on the ADA activity at the begginning of the application time. On the other hand this inhibition was there replaced by activation after 16 th hour and statistically significant increase (in the ADA enzyme activity) was found in the 64 th and 72 nd hours.Öğe Parquat’ın farelerin (Mus musculus-swiss albino) farklı dokularında adenozin deaminaz aktivitesi üzerine etkisi(Cumhuriyet Üniversitesi, 1996) Polat, Fikriye; Dere, EgemenII ÖZET Yüksek Lisans Tezi Paraquat'm Farelerin (Muş ınusculus-S\v\ss Albino) Farklı Dokularında Adenozin Deaminaz Aktivitesi Üzerine Etkisi Fikriye POLAT.* Cumhuriyet Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Danışman : Yrd.Doç. Dr. Egemen DERE Bu çalışmada, bir herbisit olan Paraquat'ın LDjQ (20 mg-kg"1) dozu Muş musculus - svviss albino farelere intrapeıitonal yoldan uygulanarak 2, 4, 8, 16, 32, 64 ve 72 saat sonra hayvanların karaciğer, akciğer ve böbreklerinde adenozin deaminaz aktivitesi üzerine etkisi araştırılmıştır. Paraquat, karaciğer ve böbrek adeııoziıı deaminaz aktivitelerinde önemli bir değişiklik göstermemiştir. Karaciğer adenozin dearninaz'ı kontrolle sürekli bir parelellik gösterirken, böbrek adenoziu deaminaz'ında önemsiz iniş-çıkışlar görühnüştür. Akciğerde ise, adenozin deaminaz enzim aktivitesinde başlangıçta önemli bir inhibisyon oluşurken, 16. saatten sonra bu inhibisyon yerini aktivasyona bırakmış, 64 ve 72. saatlerde istatistiksel olarak önemli bir artış bulunmuştur. Anahtar Kelimeler : Paraquat, Adenozin Deaminaz
