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Öğe Comparative effects of riboflavin, nicotinamide and folic acid on alveolar bone loss: A morphometric and histopathologic study in rats(SRPSKO LEKARSKO DRUSTVO, 2016) Akpinar, Aysun; Karakan, Nebi Cansin; Alpan, Aysan Lektemur; Dogan, Suat Serhan Altintepe; Goze, Fahrettin; Poyraz, OmerIntroduction Periodontitis is a chronic inflammatory and osteolytic disease. Vitamin B complex is a class of water-soluble vitamins that play important roles in cell metabolism. Objective The aim of this study was to evaluate the effects of riboflavin (RBF), nicotinamide (NA), and folic acid (FA) on alveolar bone loss in experimental periodontitis rat model. Methods Sixty-four male Wistar rats were randomly divided into the following eight groups: Control, Ligated, RBF50 (RBF, 50 mg/kg daily), NA50 (NA, 50 mg/kg daily), FA50 (FA, 50 mg/kg daily), RBF100 (RBF, 100 mg/kg daily), NA100 (NA, 100 mg/kg daily), and FA100 (FA, 100 mg/kg daily). Periodontitis was induced using silk ligature around the right first mandibular molar. After 11 days the rats were sacrificed. Mandible and serum samples were collected. Changes in alveolar bone levels were measured clinically, and periodontal tissues were examined histopathologically. Serum IL-1 beta (pg/ml) levels were analyzed by using ELISA. Results Mean alveolar bone loss in the mandibular first molar tooth revealed to be significantly lower in RBF100 group than in the Control group. In the Ligated group, alveolar bone loss was significantly higher than in all other groups. The ratio of presence of inflammatory cell infiltration in the Ligated group was significantly higher than in the Control group. The differences in the serum IL-1 beta levels between the groups were not statistically significant. Osteoclasts that were observed in the Ligated group were significantly higher than those of the Control and FA100 groups. The osteoblastic activity in the Ligated group, RBF100, and NA100 groups were shown to be significantly higher than those in the Control group. Conclusion This study has demonstrated that systemic administration of RBF, NA, and FA in different dosages (50-100 mg/kg) reduced alveolar bone loss in periodontal disease in rats.Öğe Crimean-Congo Hemorrhagic Fever Virus in High-Risk Population, Turkey(CENTERS DISEASE CONTROL, 2009) Gunes, Turabi; Engin, Aynur; Poyraz, Omer; Elaldi, Nazif; Kaya, Safak; Dokmetas, Ilyas; Bakir, Mehmet; Cinar, ZiynetIn the Tokat and Sivas provinces of Turkey, the overall Crimean-Congo hemorrhagic fever virus (CCHFV) seroprevalence was 12.8% among 782 members of a high-risk population. CCHFV seroprevalence was associated with history of tick bite or tick removal from animals, employment in animal husbandry or farming, and being > 40 years of age.Öğe Crimean-Congo Hemorrhagic Fever Virus in Ticks Collected from Humans, Livestock, and Picnic Sites in the Hyperendemic Region of Turkey(MARY ANN LIEBERT INC, 2011) Gunes, Turabi; Poyraz, Omer; Vatansever, ZatiDuring June and July 2007, about 3125 adult ticks were collected from humans, animals, and vegetation in a hyperendemic region (Sivas and Tokat) of Turkey. A total of 2193 ticks were pooled in 225 pools and screened for the Crimean Congo hemorrhagic fever virus (CCHFV) presence by antigen-capture enzyme-linked immunosorbent assay. Infection rates were calculated as the maximum likelihood estimation with 95% confidence intervals (CI). The dominant tick species was found to be Hyalomma marginatum with the following infestation rates in human, cattle and sheep, respectively: 47.43%, 66.07%, and 30.12%. Maximum likelihood estimation values of CCHFV in H. marginatum ticks collected from human, cattle, and sheep were 0.91% (CI 0.05-4.42), 2.10% (CI 1.12-3.64), and 3.11% (CI 1.18-6.87), respectively. CCHFV antigens were also demonstrated in Hyalomma excavatum, Haemaphysalis parva, and Boophilus annulatus ticks collected from cattle and Rhipicephalus bursa ticks from sheep. Our results suggest that the studied area might maintain its endemic properties in the near future unless effective tick control measures are implemented.Öğe Cystic echinococcosis: a study of consciousness and creating awareness(2007) Ozçelik, Semra; Kengeç, Lütfü; Celiksöz, Ali; De?erli, Serpil; Ataş, Ahmet Duran; Poyraz, OmerStudy was started in November 2005 and education practices and other activities have been realized between the dates of December 2005 and 5 January 2006. A wide group has been chosen from the students who are at the level of primary and secondary education to adults as a target mass to create awareness. Correspondences related to the subject have been done with Cumhuriyet University rector ship, Sivas governs ship, Sivas manager of education, Sivas mufti and essential otorizations got. Pictorial leaflets which introduce disease and explain infection and ways of protection are prepared and sent all province and county school by means of Manager of Education. Besides, lots of leaflet is distributed to tradesmen and public. Large sized posters are published about disease to make people aware of it. It is provided that these posters are distributed to all schools and mosques. Lectures are organized to introduce the disease and emphasize the ways of protection in five fixed center by two sessions. It is provided that 89 primary and secondary school students' attendance regularly with their teachers. Besides the working of society awareness, preparing executing and treating of control programs about cystic echinococcosis are other important and necessary stages.Öğe Effect of meloxicam on gingival crevicular fluid IL-1beta and IL1 receptor antagonist levels in subjects with chronic periodontitis, and its effects on clinical parameters(SPRINGER HEIDELBERG, 2006) Toker, Hulya; Marakoglu, Ismail; Poyraz, OmerThe aim of the present study was to determine the effects of meloxicam after initial periodontal treatment on interleukin-1beta (IL-1 beta) and IL-1 receptor antagonist (IL-1ra) in gingival crevicular fluid (GCF) and clinical parameters in the chronic periodontitis patients. Data were obtained from 30 patients with chronic periodontitis. Fifteen chronic periodontitis patients received 7.5 mg meloxicam, and 15 patients received placebo tablets in a 1x1 regimen for 1 month. All subjects were nonsmokers and had not received any periodontal therapy. The plaque index (PI), gingival index (GI), probing depth (PD), and clinical attachment level (CAL) were recorded. The GCF was collected using a paper strip: eluted and enzyme-linked immunoabsorbent assays (ELISAs) were performed to determine the cytokine levels. The clinical data and GCF samples were obtained after periodontal therapy and 1 month after periodontal therapy. The PI, GI, PD, and GCF IL-1ra decreased significantly (p < 0.05) in meloxicam group at first month when comparing the initial levels. While decrease of the PI was statistically significant in control group (p < 0.05), statistically significant changes were not determined in the other clinical parameters and GCF cytokine levels (p > 0.05). There were no significant differences between two groups in any of the investigated parameters. Our observations did not reveal any influence of meloxicam on levels of IL-1 beta and IL-1ra in chronic periodontitis. Additional clinical studies are advisable to determine whether COX-2 selective drugs alter periodontal disease outcome with greater safety.Öğe Effect of Non surgical Periodontal Treatment on Gingival Crevicular Fluid Interleukin-1beta and Interleukin-37 in Different Periodontal Diseases(Galenos Yayincilik, 2021) Gorgun, Emine Pirim; Toker, Hulya; Poyraz, OmerObjective: Interleukin-37 (IL-37), which is a natural inhibitor of innate immunity, is correlated with chronic inflammation and some autoimmune diseases. Proinflammatory cytokines, for example, interleukin-1beta (IL-1 beta), which plays a key part in the periodontium, can induce IL-37. This study aimed to investigate the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) IL-37 and IL-1 beta levels in generalized aggressive periodontitis (G-AgP) and generalized chronic periodontitis (GCP). Materials and Methods: Thirty-five patients with G-AgP, 30 patients with GCP and 30 volunteers with healthy periodontal status (C) were included in this research. Patient groups received scaling and root planning with periodontal hand instrumentations. Clinical periodontal parameters and GCF samples were collected at baseline and at six weeks following SRP. Biomarker levels in GCF were analysed by enzyme-linked immunosorbent assay. Results: Both periodontitis groups exhibited significant improvement in clinical parameters (p<0.05). GCF IL-37 levels were significantly higher in G-AgP and GCP groups than in the C group at baseline (p<0.05). Following periodontal treatment, GCF IL-37 levels were decreased and comparable in all groups. The GCF IL-1 beta levels were significantly higher at baseline in the G-AgP group than those in GCP and C groups, and after periodontal therapy, a decrease was detected in GCF IL-1 beta levels. GCF levels of IL-1 beta were positively correlated with GCF IL-37 levels. Conclusion: Clinical improvement was found in both periodontitis groups after periodontal treatment. IL-1 beta is a key regulator of periodontal inflammation and bone resorption. According to the results of our research, IL-37 may be useful marker of determining the clinical health and disease status of patients with periodontitis. However, further studies are required to determine the mechanism of IL-37 in periodontal inflammation.Öğe Effect of periodontal treatment on IL-1 beta, IL-1ra, and IL-10 levels in gingival crevicular fluid in patients with aggressive periodontitis(WILEY, 2008) Toker, Hulya; Poyraz, Omer; Eren, KayaAim:The aim of this study was to examine the effect of phase I periodontal treatment on the levels of interleukin (IL)-1 beta, IL-1ra, and IL-10 in gingival crevicular fluid (GCF) in patients with generalized aggressive periodontitis (G-AgP). Material and Methods: Data were obtained from 15 patients with aggressive periodontitis and 15 healthy controls. GCF was collected from at least four pre-selected sites (one shallow, at least two moderate, or at least one deep pockets) in patients with G-AgP. In the healthy group, GCF samples were collected from one site. The cytokine levels were determined by an enzyme-linked immunosorbent assay. Probing depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. The GCF sampling and clinical measurements were recorded at baseline and 6 weeks later after periodontal treatment. Results: IL-1 beta levels were significantly higher at the moderate and deep pocket sites compared with the shallow sites (p < 0.05). After periodontal therapy, IL-1 beta levels were significantly reduced in the moderate and deep pocket sites (p<0.05). IL-1ra levels at baseline of the moderate and deep pocket sites were significantly lower than the control sites (p < 0.05). IL-10 levels were similar in all pockets and did not change after periodontal therapy. Conclusions: The periodontal treatment improves the clinical parameters in G-AgP, and this improvement is evident in deep pocket sites for pocket depth and CAL values. These results confirm that IL-1 beta is effective for evaluating the periodontal inflammation and can thus be used as a laboratory tool for assessing the activity of periodontal disease.Öğe Effects of Curcumin on Alveolar Bone Loss in Experimental Periodontitis in Rats: A Morphometric and Histopathologic Study(VERLAG HANS HUBER, 2017) Akpinar, Aysun; Calisir, Metin; Karakan, Nebi Cansin; Alpan, Aysan Lektemur; Goze, Fahrettin; Poyraz, OmerBackground: Curcumin is found in the rhizomes of the turmeric plant that has been showed antioxidant and anti-inflammatory effect. The aim of this study was to evaluate the effects of systemic curcumin therapy on alveolar bone loss in an experimental periodontitis model in rats. Material and Methods: Thirty-two male Wistar rats were randomly divided to 4 groups: 75 mg/kg/daily curcumin (C75; n = 8), 150 mg/kg/daily curcumin (C150; n = 8), Control (n = 8), and Ligated (n = 8). Curcumin was administrated using gastric gavage. After 12 days, the rats were sacrificed. Right mandibles samples were histopathologically examined. Alveolar bone loss was measured. Interleukin 1 beta (IL-1 beta) and interleukin 10 (IL-10) were evaluated in the serum samples and gingival homogenates. Results: The measurements of alveolar bone loss in the mandibular molars revealed significantly higher bone-loss values in the Ligated group than the Control, C75 and C150 groups. The IL-1 beta levels in the gingival homogenates were significantly increased in the Ligated group compared to those of the Control, C75 and C150 groups. The serum IL-1 beta levels in the Ligated group were significantly higher than the Control group. The mean osteoblast numbers in the Ligated group were lower than those of the Control, C75 and C150 groups. The C150 groups showed significantly more osteoblasts than the Control group. The osteoclast numbers in the Ligated group increased significantly compared to the C75, C150 and control groups. Conclusion: This study demonstrates that systemic administration of curcumin at the 75 and 150mg/kg doses reduced alveolar bone loss in the periodontal disease in rats.Öğe The effects of IL-10 gene polymorphism on serum, and gingival crevicular fluid levels of IL-6 and IL-10 in chronic periodontitis(UNIV SAO PAULO FAC ODONTOLOGIA BAURU, 2018) Toker, Hulya; Gorgun, Emine Pirim; Korkmaz, Ertan Mahir; Yuce, Hatice Balci; Poyraz, OmerObjective: Anti-inflammatory cytokines play a crucial role in periodontitis by inhibiting synthesis of pro-inflammatory cytokines. The purpose of this study was to evaluate the effect of interleukin-10 (-597) gene polymorphism and genotype distributions on chronic periodontitis (CP) development and IL-6 and IL-10 levels in gingival crevicular fluid (GCF) and serum before and after non-surgical periodontal treatment. Material and Methods: The study population consisted of 55 severe generalized CP patients as CP group and 50 healthy individuals as control group. Plaque index, gingival index, probing depth and clinical attachment level were recorded and GCF and blood samples were taken at both the baseline and the sixth week after non-surgical periodontal treatment. PCR-RFLP procedure was used for gene analyses and cytokine levels were measured via ELISA. Results: IL-10 genotype distribution was significantly different between CP and control groups (p=0.000, OR: 7, 95% CI, 2.83-60.25). Clinical measurements significantly improved in the CP group after periodontal treatment (p<0.05). Periodontal treatment significantly decreased GCF IL-6 and IL-10 levels. No significant difference was found in clinical parameters between IL-10 AA and AC+CC genotypes at both the baseline and the sixth week (p>0.05). Sixth week GCF IL-10 levels were significantly lower in patients carrying IL-10 AC+CC genotype compared to the patients carrying IL-10 AA genotype (p<0.05). Serum IL-6 and IL-10 levels were lower in patients carrying the IL-10 AA genotype compared to patients with IL-10 AC+CC genotype, but the difference was not significant (p>0.05). Conclusion: IL-10 AA genotype carriers had lower IL-6 and IL-6/10 levels in serum; however, GCF IL-6/10 levels were similar in both genotypes. Within the limitations of our study, a possible association between IL-10(597) gene polymorphism and CP might be considered.Öğe Erythrocyte Sedimentation Rate, Serum Amyloid A and C-reactive Protein Levels in Patients with Acute Brucellosis(PROFESSIONAL MEDICAL PUBLICATIONS, 2011) Celik, V. Kenan; Sari, Ismail; Bakir, Sevtap; Engin, Aynur; Bakir, Deniz; Sari, Musa; Poyraz, Omer; Yildiz, GurselObjective: To determine the levels of and the relationship between serum amyloid A (SAA), C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) in patients with acute brucellosis. Methodology: SAA, CRP and ESR were measured with ELISA, nephelometry and Mix-Rate x100 vital diagnostic device, respectively, in serum samples of 30 patients diagnosed with acute brucellosis and 40 volunteers with no systemic diseases. Results: Statistically significant difference was determined between patients and controls in terms of the levels of SAA, CRP and ESR (p<0.05). Although CRP levels and ESR were significantly correlated in the patient group (p<0.05), there was no significant correlation between CRP and SAA or ESR and SAA (p>0.05). Conclusion: CRP levels are significantly elevated in bacterial infections, however the increase is minimal in viral infections. SAA increases sooner and to greater levels during the course of infections. In addition, contrary to CRP, SAA is markedly elevated in both bacterial and viral infection. Consequently, these three acute phase reactants should be used together in order to obtain more reliable results in the differential diagnosis of infections.Öğe Evaluation of IL-1 beta, IL-1ra, and IL-10 levels and outcome of periodontal therapy in chronic periodontitis with familial Mediterranean fever(SPRINGER HEIDELBERG, 2017) Bostanci, Vildan; Toker, Hulya; Senel, Soner; Poyraz, Omer; Akpinar, Aysun; Gorgun, Emine Pirim; Bakar, OlcayThis study aimed to examine the IL-1 beta, IL-1ra, and IL-10 cytokine levels in gingival crevicular fluid (GCF) and serum of familial Mediterranean fever (FMF) and chronic periodontitis (CP) patients, and their response to nonsurgical periodontal therapy. A total of 50 patients, 15 FMF patients with generalized chronic periodontitis (FMF-CP), 15 systemically healthy patients with generalized chronic periodontitis (CP), ten systemically and periodontal healthy controls (HC), and ten periodontally healthy FMF patients (FMF-HC) were enrolled in the study. The cytokine levels in GCF and serum were determined by ELISA. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks were recorded. The study indicated statistically significant healing of the clinical parameters in both FMF-CP and CP groups after periodontal treatment. GCF IL-1 beta levels at 6 weeks in FMF-CP group were significantly lower than the CP group (p < 0.05), and GCF IL-1ra levels were significantly decreased at 6 week in the FMF-CP group (p < 0.05). GCF IL-10 levels were significantly higher in the FMF-CP group than in the other groups at baseline and 6 weeks (p < 0.05). There were no significant differences in serum-IL-1 beta, IL-1ra, and IL-10 levels either FMF-CP or CP groups at baseline or 6 weeks (p > 0.05). The results of our study suggested that there was a positive correlation between gingival inflammation and serum cytokine levels in FMF patients and also colchicine treatment showed protective effects on GCF cytokine levels in FMF-CP group. Following treatment, GCF IL-1 beta and GCF IL-1ra levels were decreased in FMF-CP group. GCF IL-10 levels were increased in FMF-CP group compared to other groups. Also, the serum cytokine levels associated with periodontal inflammation in FMF patients.Öğe Humic Acid, a Polyphenolic Substance, Decreases Alveolar Bone Loss in Experimental Periodontitis in Rats(Sage Publications Inc, 2019) Calisir, Metin; Akpinar, Aysun; Poyraz, Omer; Goze, Fahrettin; Cinar, ZiynetThe purpose of this study was to evaluate the biochemical, morphometric, and histopathological changes associated with experimental periodontitis in rats in response to local administration of humic acid. Thirty-eight Wistar rats were divided into 5 experimental groups: nonligated (NL) group, ligature-only (LO) group, and ligature + local administration of humic acid (20, 80, and 150 mg/kg body weight per day for 15 days, respectively; L-20, L-80, and L-150 groups). Changes in alveolar bone levels were clinically measured as the distance from the cementoenamel junction to the alveolar bone crest with a stereomicroscope. Tissues were histopathologically examined to assess the osteoclast numbers, osteoblastic activity, and inflammatory cell infiltration among the study groups. Enzyme-linked immunosorbent assay interleukin1 beta (IL-1 beta) and IL-10 levels in serum and gingival homogenates were evaluated. At the end of 15 days, the alveolar bone loss was significantly higher in the LO group compared to the NL, L-20, and L-150 groups (P < .05). The osteoclast number in the LO group was significantly higher than the NL, L-20, and L-150 groups (P < .05). Inflammatory cell infiltration was significantly higher in the LO and L-80 groups than the other groups (P < .05). The highest serum and gingival homogenate IL-10 levels were determined in the NL group (P < .05). The serum and gingival homogenate IL-1 beta levels in LO group were significantly higher than the NL, L-20, and L-150 groups (P < .05). Within the limits of this study, it can be suggested that humic acid, when administered locally at 20 and 80 mg/kg doses, may prevent alveolar bone loss in the rat model.Öğe IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters(SOCIEDADE BRASILEIRA DE PESQUISA ODONTOLOGICA, 2017) Gorgun, Emine Pirim; Toker, Hulya; Korkmaz, Ertan Mahir; Poyraz, OmerGenetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin (IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid (GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). GAgP patients showed significant improvement on clinical parameters after periodontal therapy (p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levels were observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6 (-174) and IL-10 (-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6 (-174) GG genotype had higher levels of GCF IL-10 levels.Öğe Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment(WILEY-BLACKWELL, 2012) Toker, Hulya; Akpinar, Aysun; Aydin, Huseyin; Poyraz, OmerToker H, Akpinar A, Aydin H, Poyraz O. Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment. J Periodont Res 2012; 47: 572577. (C) 2012 John Wiley & Sons A/S Background and Objective: The aim of this study was to evaluate the impact of smoking on the relationship between interleukin-1 (IL-1 beta) and oxidation in patients with periodontitis and response to nonsurgical periodontal therapy. Material and Methods: Data were obtained from 30 patients with generalized chronic periodontitis (15 smokers and 15 nonsmokers) and from 10 periodontally healthy controls. IL-1 beta level, total oxidant status (TOS) and total antioxidant status (TAS) were recorded in gingival crevicular fluid. Probing depth, clinical attachment level, gingival and plaque indices and bleeding on probing were also measured. The gingival crevicular fluid and clinical parameters were recorded at baseline and 6 wk after periodontal treatment. Results: The study showed statistically significant improvement of clinical parameters in both smokers and nonsmokers after periodontal treatment. Moreover, the baseline IL-1 beta levels were significantly higher in smokers compared with nonsmokers (p < 0.05). After periodontal treatment, the IL-1 beta levels were significantly reduced in both smokers and nonsmokers (p < 0.05). There were no significant differences in TOS and TAS between periodontitis patients and healthy controls at baseline and 6 wk after periodontal treatment. The level of IL-1 beta in gingival crevicular fluid was positively correlated with TOS in both smokers and nonsmokers. Conclusions: Periodontal treatment improved the clinical parameters in both smokers and nonsmokers. The results confirm that periodontal therapy has an effect on IL-1 beta levels in gingival crevicular fluid, but not on TOS and TAS.Öğe Investigating the Effects of Systemically Administered Strontium Ranelate on Alveolar Bone Loss Histomorphometrically and Histopathologically on Experimental Periodontitis in Rats(AMER ACAD PERIODONTOLOGY, 2017) Karakan, Nebi Cansin; Akpinar, Aysun; Goze, Fahrettin; Poyraz, OmerBackground: The aim of this study is to investigate effects of strontium ranelate (SR) on alveolar bone loss (ABL) in rats with experimental periodontitis. Methods: Forty Wistar rats were randomly divided into five groups: 1) control (n = 8); 2) ligated (n = 8); 3) 300 mg/kg SR (SR300, n = 8); 4) 625 mg/kg SR (SR625, n = 8); and 5) 900 mg/kg SR (SR900, n = 8). To create experimental periodontitis, 4/0 silk ligatures were inserted submarginally around first molars at the right mandible. After 11 days, rats were sacrificed. ABL was calculated by measuring cemento-enamel junction and alveolar crest distance. Interleukin (IL)-1 beta, osteoprotegerin (OPG), and bone-specific alkaline phosphatase (BALP) serum levels were determined by enzyme-linked immunosorbent assay. Histopathologic analysis was used to evaluate inflammatory cell infiltration, numbers of osteoblasts and osteoclasts, and receptor activator of nuclear factor-kappa B ligand (RANKL) activity. Results: ABL was significantly lower in SR900 group than in the ligated group (P <0.05). Osteoclast numbers in ligated group were significantly higher than in the control, SR300, and SR900 groups (P < 0.05). In ligated, SR625, and SR900 groups, significantly higher osteoblast numbers were detected than in control group (P < 0.05). Osteoblast numbers in SR625 group were significantly higher than in the SR300 group (P <0.05). RANKL activities in SR900 and control groups were close to each other (P >0.05). Serum IL- 1 beta, OPG, and BALP levels revealed no significant difference (P >0.05). Conclusion: It can be concluded that SR can reduce RANKL activity and osteoclast numbers, as well as ABL.Öğe Investigation of Chlamydophila pneumoniae Seropositivity and Risk Factors in Patients with Atherosclerotic Vascular Disease in Sivas, Turkey(ANKARA MICROBIOLOGY SOC, 2012) Kilic, Zeliha Banu; Poyraz, Omer; Kilic, Ahmet TurhanThe role of various microorganisms including Chlamydophila (formerly Chlamydia) pneumoniae, have been frequently investigated in the pathogenesis of atherosclerosis. In our study, the relationship between C.pneumoniae seropositivity and risk factors for atherosclerosis have been evaluated. A total of 90 atherosclerotic patients (71 of them were male; age range: 45-87 years; mean age: 65.3 +/- 8.7 years) and 90 control subjects without coronary diseases (41 of them were male; age range: 42-84 years; mean age: 61.6 +/- 9.6 years) were included in the study. Both groups were also evaluated for the presence of risk factors such as age, gender, smoking, hypertension, diabetes, obesity, dyslipidemia, familial history and the high levels of ferritin, cholesterol (total, HDL and LDL) and HS (high sensitive)-CRP. The presence of C.pneumoniae IgG, IgM and IgA antibodies were investigated by micro-immunofluorescence (MIF) and ELISA methods using commercial kits (Euroimmun, Germany). The total antibody seropositivity rate was found 100% (90/90) in patient group by both MIF and ELISA methods, while this rate in control group was 94% (85/90) by MIF and 92% (83/90) by ELISA. When MIF test results were taken into consideration (since it is accepted as the reference method for C.pneumoniae serology), IgG, IgM and IgA positivity rates in patient and control groups were found as 100% (90/90) and 89% (80/90); 70% (63/90) and 59% (53/90); 3% (3/90) and 2% (2/90), respectively. Statistically (s)ignificant difference between patient and control groups was detected only for IgG positivity (p < 0.05) and for total antibody positivities (100% and 94%, respectively) (p < 0.05). The evaluation of the risk factors revealed that age, hypertension, dyslipidemia and HS-CRP levels exhibited statistically significant differences between patient and control groups (p < 0.05 for each parameter tested). Statistically significant relation was detected only between high HS-CRP levels and C.pneumoniae seropositivity (p < 0.05). It was concluded that in areas with high C.pneumoniae infection prevalence, early diagnosis and specific treatment of C.pneumoniae infections, may prevent establishment of chronic infection and eliminate a risk factor for the development of atherosclerosis.Öğe The Investigation of Cryptosporidium spp. Prevalence in Cattle and Farmers by ELISA(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2012) Ozcelik, Semra; Poyraz, Omer; Kalkan, Kadir; Malatyali, Erdogan; Degerli, SerpilCryptosporidium spp. is an important cause of enteritis for animals and humans. As the parasite has zoonotic potential, it is aimed to investigate the prevalence in people who had close contact with livestock, in control group who lived in Centrum and cattle. Fecal samples collected from 50 farmers who lived in different villages of Sivas and 65 people to be control group lived in Sivas Center without gastrointestinal discomfort and 200 cattle and calf breeding different county during the period from September 2009 to September 2010. Fecal samples were stored in 10% formalin until examination and then studied with Cryptosporidium Stool Antigen Microwell ELISA Kit. As a result, positive rate were found 4(6.2%) in control group and 9(18%) in study group, (chi(2)=3.95, P<0.05). On the other hand, 15 (7.5%) of the cattle and calve samples gave positive reaction with Cryptosporidium spp. ELISA.Öğe Investigation of the Presence of Francisella tularensis and Acanthamoeba spp. in the Drinking Water of Sivas Province, Turkey(Bilimsel Tip Yayinevi, 2021) Atas, Mehmet; Poyraz, OmerIntroduction: Tularaemia is a zoonotic disease caused by Francisella tularensis. F. tularensis subsp. holarctica is primarily isolated in Northern Europe, the Balkans, Turkey, Siberia, and the Far East, and it is mostly responsible for waterborne outbreaks. F. tularensis, which is a facultative intracellular bacterium, may survive inside Acanthamoeba castellani, which is a free-living amoeba in water resources; this symbiotic life allows the bacteria to survive in water and mud for months. In this study, drinking water samples taken during the tularaemia outbreak and non-outbreak regions were compared in terms of the presence of Francisella tularensis and Acanthamoeba spp., and the role of Acanthamoeba was investigated in tularemia outbreaks observed in Turkey. Materials and Methods: This study was conducted in the province of Sivas located in the central Anatolia region in Turkey, and a total of 300 water samples were used. Glucose-cysteine-blood agar (GCBA) and non-nutrient agar (NNA) were used for the isolation of F. tularensis and Acanthamoeba, respectively. Each isolate was identified by PCR technique. Results: A total of 30 (10%) F. tularensis and 28 (9.33%) Acanthamoeba spp. isolates were produced by culture method. All bacterial strains were confirmed with PCR as to be F. tularensis subsp. holarctica. In our study, concurrent presence of F. tularensis and Acanthamoeba were detected in two water samples. There were no statistically significant differences between the water samples taken from outbreak and non-outbreak regions in terms of the presence of Acanthamoeba (p> 0.05). Conclusion: Even though experimental studies have shown that F. tularensis may survive inside Acanthamoeba spp. and its viability continues, our study could not identify a relationship between tularemia outbreak and Acanthamoeba spp. New research on this issue may have a contribution on tularemia epidemiology.Öğe [Seroprevalance of Babesia microti in humans living in rural areas of the Sinop region].(2010) Poyraz, Omer; Güneş, TurabiBabesia species are blood parasites which transmitted by ticks and located in erythrocytes. Babesia divergens in Europe and Babesia microti in America are the most significant etiologic agents of human babesiosis cases. The aim of this study was to determine serologically whether people living in Sinop where Ixodes ricinus ticks are commonly seen have been exposed to B. microti or not. For this purpose, during May and June of 2006-2007, blood specimens were obtained from 273 individuals who lived in villages of the Sinop region. B. microti IgG antibodies were investigated by the indirect fluorescent antibody test in sera obtained from blood specimens. B. microti seropositivity was found in 6.23% of these sera. There was no statistical difference in antibody seropositivity rates according to sex groups, age groups, occupational groups and recall of tick bites (p > 0.05). This study is the first seroepidemiologic report of B. microti antibodies in people living in Turkey.Öğe The seroprevalence of Anaplasma phagocytophilum in humans from two different climatic regions of Turkey and its co-seroprevalence rate with Borrelia burgdorferi(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2011) Gunes, Turabi; Poyraz, Omer; Atas, Mehmet; Turgut, Nergiz HacerAim: To investigate the seroprevalence of Anaplasma phagocytophilum in people living in 2 different climatic regions and to evaluate the co-seroprevalence rate of A. phagocytophilum with Borrelia burgdorferi. Sinop and Tokat provinces, both in the middle Black Sea region of Turkey, have distinct climatic features. Materials and methods: In 2006-2007, serum samples were collected from people living in rural areas of Tokat and Sivas, and anti-A. phagocytophilum IgG antibodies were explored by the IFA method. Positive samples were further investigated for the possible presence of B. burgdorferi IgG antibodies. Results: A. phagocytophilum seropositivity was found in 29 (10.62%) out of 273 serum samples in Sinop and 21 (5.77%) out of 364 serum samples in Tokat (P = 0.035). Co-seroprevalence for A. phagocytophilum and B. burgdorferi was found to be 3.30% in Sinop and 0.55% in Tokat (P = 0.012). Conclusion: The current study suggests that A. phagocytophilum infections can be seen in humans from different climatic regions of Turkey. Both the seroprevalence of A. phagocytophilum and the possibility of mixed infections of A. phagocytophilum and B. burgdorferi are higher in places with more suitable habitats for Ixodes ricinus ticks.
