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Öğe Apical extrusion of intracanal biofilm using ProTaper gold, wave one gold,twisted file adaptive, oneshape new generation and K3XF(Kare Publishing, 2016) Zan, Recai; Tunç, Tutku; Hubbezoglu, İhsan; Sümer, ZeynepObjective: To evaluate the bacterial extrusion during instrumentation with different nickel titanium (NiTi) engine-driven instruments. Methods: Ninety extracted single-canal human mandibular incisor teeth were inoculated with Enterococcus faecalis to obtain biofilm formation and were randomly divided to 6 groups (n=15). One group served as the control and was not instrumented; the other groups were prepared with ProTaper Gold (PTG; Dentsply Maillefer, Ballaigues, Switzerland), WaveOne Gold (WOG; Dentsply Maillefer), Twisted File Adaptive (TFA; SybronEndo, Orange, CA, USA), One Shape New Generation (OSNG; MicroMega, Besancon, France), and K3XF (SybronEndo) instruments. Bacteria extruded beyond the apical foramen were quantified in colony-forming units per milliliter. The number of colony-forming units in the remaining biofilm was determined for each sample. Data were analyzed using the one-way analysis of variance (ANOVA) and Tukey post-hoc tests. Results: All NiTi instruments resulted in different quantities of bacterial extrusion. The TFA group caused most bacterial extrusion (p<0.05). The PTG and WOG groups caused less bacterial extrusion than the OSNG and K3XF groups (p<0.05), but there was no statistically significant difference between the PTG and WOG groups (p>0.05). Conclusion: PTG and WOG are preferable system in terms of successful endodontic treatments. The amount of bacterial extrusion is associated with the metallurgy and design of the instrument used. © 2016 Kare Publishing. All rights reserved.Öğe Can garlic (Allium sativum) extract be used as scolocidal agent?(2007) Ozçelik, Semra; Sümer, Zeynep; De?erli, Serpil; Ozan, Fatih; Sökmen, AtalayGarlic (Allium sativum L.) has been used in the public health in some of treatments for years. The most important chemical compounds of garlic are sulphide (alisin, ajoen and diallysulphure) compounds. Allisin is described as antibacterial, antifungal, antiparasitic and anticarsinogenic agent and many works practice on it. But its antiscolocidal effect has not been worked yet. In our work, whether garlic extract has effects both direct and daughter vesicules to protoscolex is searched. In the study during the surgery daughter vesicules and protoscolex which are acquired from liver cyst hydatique and extracts which obtained from garlic grown in the Kastamonu region are used. Viability determination is work at using 0.1% eozin solution. In 50% mg/ml concentration garlic extract to protoscolex' in 15 minutes, in 25% mg/ml concentration 20. minutes and 12.5% mg/ml concentration in 30 minutes have full effect. While it has full effect in 20. minutes to protoscolex into the vesicules within the 50% mg/ml 12.5 mg/ml concentration, there has been 80% viability in 40 minutes.Öğe Examination of Biological Activity of Passiflora edulis (Çarkıfelek) Extract via Phytochemical Analysis(2024) Tunc, Tutku; Sümer, Zeynep; Öksüz, Kerim Emre; Öksüz, Kerim EmreIn recent years, the use of medicinal plants as sources of drugs or herbal extracts has been of great importance. Passiflora edulis is nowadays widely studied for its antimicrobial, anticancer, and antioxidant potential. Therefore, this study aimed to determine the phytochemical structure of ethanol extract of P. edulis leaves and to investigate its biological properties such as antimicrobial and anticancer activities. The ethanol extract of P. edulis leaves was obtained and analyzed by GC-MS. The antimicrobial activity of P. edulis leaf extract was determined by MIC test. XTT method was used to determine the antiproliferative activity. In the phytochemical analysis of P. edulis extract, dodecanoic acid, tetradecanoic acid, and n-hexadecanoic acid were found the most. The antimicrobial effect of P. edulis leaf extract was found against pathogenic microorganisms. In addition, P. edulis leaf extract was found to have high anticancer activity against OvCar and MCF-7 cell lines, while it had the highest effect on the PC-3 cell line. It is thought that the effectiveness of this antiproliferative and antimicrobial activity is related to the secondary metabolites determined by GC-MS analysisÖğe İçme ve Kullanma Suyu Örneklerinin Mikrobiyolojik Kalitesinin Escherichia coli O157:H7 Serotipi Yönünden Araştırılması: Sivas İli Örneği(Sivas Cumhuriyet University, 2021) Aslan, Rukiye; Sümer, ZeynepBu çalışma Sivas ili içme ve kullanma sularının koliform grubu Escherichia coli bakterisi O157:H7 serotipi yönününden incelenmesi amacıyla yapılmıştır. Bu amaçla Sivas ili ve ilçelerindeki farklı kaynaklardan 200 adet içme ve kullanma suyu örneği “Su Kirliliği Kontrolü Yönetmeliği (SKKY) Numune Alma ve Analiz Metodları Tebliği” ve “İnsani Tüketim Amaçlı Sular Hakkında Yönetmelik (İTASHY)” standartları referans alınarak toplanmış ve su örneklerinin analizi “TS EN ISO 9308-1 Escherichia coli ve Koliform Bakterilerin Tespiti ve Sayımı: Membran Filtrasyon Yöntemi” referans alınarak çalışılmıştır. Analiz sonrası üreme gösteren koliform ve fekal koliform bakterilerle E. coli bakterisinin doğrulanması amacıyla biyokimyasal testler uygulanmıştır. E. coli O157:H7 serotipi tespiti için SMAC (Sorbitol MacConkey) Agar ve Escherichia coli O157:H7 lateks aglütinasyon kiti kullanılmıştır. İçme ve kullanma suyu örneklerinde %40 koliform bakteri ve %33 E. coli bakterisi tespit edilmiştir. SMAC agardaki kültür sonuçlarına göre, örneklerden 3 tanesi E. coli O157:H7 açısından şüpheli bulunmuş ancak test tekrarları sonucunda su örneklerinde E. coli O157:H7 serotipine rastlanmamıştır. İncelenen içme ve kullanma suyu örneklerinde E. coli bakterisinin O157:H7 serotipine rastlanmasa da, fekal kontaminasyon göstergesi olan koliform bakterilerin ve E. coli bakterisinin bulunmasının sulardaki mikrobiyolojik kirlenme kaynaklı enfeksiyonlara yol açabileceği, bu durumun halk sağlığı açısından olası risk oluşturabileceği, bu amaçla kullanılacak suların tüketilmeden önce yeterli düzeyde dezenfekte edilmesi gerektiği ve dezenfeksiyonun etkinliğinin kontrol edilmesi gerektiği görüşlerine ulaşılmıştır. İçme ve kullanma sularının mikrobiyolojik kalitesinin devamı adına toplumun her kesiminin bilinçlendirilmesi ve su hijyenin sağlanması adına her bireyin etkin rol alması gerektiği düşünülmektedir.Öğe Kene Isırma Öyküsü Olan Hastalarda Kırım-Kongo Kanamalı Ateşi Virüsünün ve Coxiella burnetii, Borrelia burgdorferi Seropozitifliğinin Araştırılması(2022) Topcu, Kübra Fırtına; Sümer, ZeynepAmaç: Kene ısırma olguları bölgemizde sıklıkla görülmektedir. Çalışmamızda, kene ısırma öyküsü olan hastalarda Kırım-Kongo Kanamalı Ateşi virüsü, Coxiella burnetii ve Borrelia burgdorferi etkenlerinin araştırılması amaçlandı. Yöntem: Kene ısırması nedeniyle Mayıs-Eylül 2019 tarihleri arasında hastaneye başvuran hastaların kan örnekleri kullanıldı. Kırım-Kongo Kanamalı Ateşi (KKKA) için polimeraz zincir reaksiyonu, C. burnetii için ELISA, B. burgdorferi için ELISA ve Western blot yöntemleri kullanılarak etkenler araştırıldı. Bulgular: Anamnezinde kene ısırma öyküsü olan 84 hasta örneği kullanıldı. Olguların %35.7’si kadın, %64.3’ü erkekti. Tamamı 18 yaş üzerindeydi. Olguların %84.5’i çiftçilik-hayvancılık ile uğraşıyordu. Trombositopeni %84.5’inde vardı ve %66.7’si kene temasından sonra en erken yedi gün içinde başvurmuştu. Örneklerin %76.2’sinde KKKA pozitifti ve %8.3’ünde C. burnetii faz II IgM, %34.5’inde faz II IgG pozitifti. B. burgdorferi taraması için yapılan ELISA testinde örneklerin %3.6’sında IgM, %6’sında IgG pozitifti. Doğrulama testinde B. burgdorferi ELISA IgM pozitif örneklerin %66.7’si pozitif iken, ELISA IgG pozitif örneklerin tamamı negatif bulundu. Örneklerin %9.3’ünde C. burnetii IgM ve KKKA, %32.8’inde C. burnetii IgG ve KKKA, %1.5’inde B. burgdorferi IgM ve KKKA aynı anda pozitifti. Sonuç: Sonuç olarak, kene ısırması ile başvuran hasta grubunda C.burnetii ve B.burgdorferi seropozitiflikleri, KKKA PCR pozitifliği belirlendi. Kene temasının yalnızca KKKA için değil diğer hastalıklar içinde bir işaret ve bulaş yolu olduğu akılda tutulmalıdır.Öğe Laboratuvarımızda hazırladığımız ELISA kiti ile akciğer tüberkülozunun serolojik tanısı(Cumhuriyet Üniversitesi, 1996) Sümer, Zeynep; Poyraz, ÖmerÖZET Son yıllarda gittikçe önem kazanan, güvenilir, basit, hızlı ve tekrarlanabilir tanı yöntemlerinden olan ELISA tekniğinin tüberkülozun serolojik tanısında ne oranda etkili olabileceğini kendi laboratuvar koşullarımızda hazırladığımız kit ile, çeşitli gruplar üzerinde deneyerek saptamayı amaçladık. Çalışmada deney grubu olarak Ankara Atatürk Sanatoryumu Göğüs Hastalıkları Hastanesi' nde klinik ve laboratuvar olarak kesin akciğer tüberkülozu tanısı konmuş aktif ve kronik 99 hastadan elde edilen serum örnekleri kullanıldı. Bunların 30'u tüberküloz tanısı almış henüz tedaviye başlanmamış hastalardan, 31'i iki aydır tedavi edilmekte olan hastalardan, 3 8 'i de kronik hastalardan oluşmakta idi. Kontrol olarak PPD (-) ve PPD (+) olan 30'ar kişilik iki grup deneye alınmıştır. Hasta ve kontrol gruplarının IgG düzeylerinin ortalama absorban değerleri arasında anlamlı fark tespit edildi. MSAA antijeni ve BSA blok çözeltisi kullanıldığmda ELISA testinin duyarlılığı % 96.0, seçiciliği ise % 93.3 olarak saptandı. Yalancı pozitiflik oram % 6.7, yalancı negatiftik oram ise % 4.0 olarak bulundu. MSAA antijeni ve Jelatin blok çözeltisi kullanıldığmda ELİSA testinin duyarlılığı (% 87.0) ve seçiciliği (% 81.0) olarak tespit edildi. Yalana pozitiflik oranı % 13.0, yalancı negatiflik oranı ise % 19.0 olduğu görüldü. Oranlar arasındaki farkın istatistiksel olarak anlamlı ölçüde yüksek olduğu saptandı (p<0.01). Sonuç olarak ELISA tekniğinin; yüksek duyarlılık ve seçicilik oranlan, kısa sürede sonuç vermesi, tekrarlanabilir olması ve kullanım kolaylığı nedeniyle tüberkülozun serolojik tanısında ve tarama çalışmalarında tercih edilebilecek bir serolojik tanı yöntemi olduğu kanısındayız. 47Öğe Periodical Control of the Sausages in Term of Mold and Yeast Produced in Sivas(Selcuk University, 2012) Tutar, Uğur; Sümer, Zeynep; Yıldırım, Gülay; Çelik, CemSausage is a meat product which is consumed widely in our country. However, it is important to determine the existence of fungi widely existing in nature in sausages. Fungi are very useful for the human being in many aspects. However, they some-times may cause loss on account of the diseases they have lead to. In other words, both yeasts and molds can be dangerous for human health as they cause foods to decompose and get rotten. Moreover the responsibilities of food ethics can be put in to the agenda. This study aims to determine whether the amounts of yeast, which may have negative and permanent influ-ences, pose threat to human health. For this aim, sausage samples from the factories were taken five times a day during the 20 days’ period. After these examples were homogenized, their analysis terms of mold and yeast was made bused on the cultures from the prepared dilutions by culturing with plaque spreading method. As a consequence mold and yeast were not found in the sausage samples taken from the factories. It has been thought that the result may have occurred because of the heavy usage of protective additives and the application of temperature processing during the production of sausages and the evolvation was done in terms of food ethics.Öğe The effect of lavender oil use on pain and comfort level in intramuscular injection administration: A single blind randomized controlled study(Tissue Viability Society, 2024) Karabey, Tuba; Karagozoglu, Serife; Sümer, ZeynepContext: The fact that the concept of quality in health care services has come to the fore and that evidence-based practices have become widespread made such concepts as patient satisfaction and comfort important and priority parameters in determining the quality of health services. Objectives: The aim of the study is to determine the effects of using lavender oil in intramuscular injection on injection pain, comfort level, blood pressure, pulse rate and respiratory rate. Methods: The study was designed as a prospective, single-blind randomized controlled trial. The research was conducted in accordance with the CONSORT Checklist guide. In our study, a total of 160 individuals were included in the sample, 80 of whom were control and 80 were intervention. Structured Information Form, Visual Analog Scale and Comfort Scale were used to collect the data of the research. Mann Whitney U, Kruskal Wallis test and Wilcoxon test were used to evaluate the data. Results: It was determined that the mean pain scores of the individuals who received intramuscular injection by applying lavender oil were statistically significantly lower than the individuals in the control group. Again, the comfort score averages of the individuals who were applied lavender oil were found to be statistically significantly higher than the control group. It was determined that the blood pressure, pulse rate and respiratory rate before the injection of the individuals in the control group increased statistically significantly according to the post-injection measurements, while the blood pressure, pulse rate and respiratory rate in the lavender oil group decreased significantly after the injection and remained within their normal values. Conclusion: As a result, it was determined that the application of lavender oil in intramuscular injection had a positive and significant effect on the blood pressure, pulse rate and respiratory rate of the individuals on the injection pain and comfort level. © 2024Öğe The incidence of Demodex folliculorum in patients with chronic kidney deficiency(2007) Ozçelik, Semra; Sümer, Zeynep; De?erli, Serpil; Ozyazici, Gonca; Hayta, Sibel Berksoy; Akyol, Melih; Candan, FerhanThe aim of this study was to evaluate the prevalence of Demodex folliculorum in immune suppressed patients. The present study was carried out on individuals being treated for chronic kidney deficiency and immune suppressed patients that were hospitalized in the Hemodialysis Units and Internal Disease Service of the Cumhuriyet University Hospital. Forty-seven patients with chronic kidney deficiency (CKD) and a control group of thirty-eight healthy and actively exercising individuals were included in this investigation. By means of a questionnaire given to the patients and control group, complaints of allergenic related dermal and optical disorders were detected and the prevalence of Demodex was also evaluated by this questionnaire. First, the presence of D. folliculorum was proven taking dermal specimens from the cheek of every individual. Afterwards, at least two eyelash specimens from the lower and upper eyelid-eyelash follicles of the right and left eyes were taken and examined in a drop of olive oil between slide and cover glass microscopically. The frequency of parasites in every preparation was also determined in each positive case. D. folliculorum was found in the eyelid-eyelash follicles of 6 (12.76 %) and 12 (25.53 %) of the dermal face specimens of the 47 chronic kidney deficiency patients. On the other hand, D. folliculorum was found in the eyes of 2 (5.26 %) persons and in the dermal face specimens of 7 (18.42 %) out of a total of 38 persons in the control group. In the present study, the difference in the Demodex prevalence and complaints of dermal disorder in the two groups was found to be insignificant. In the study group, the difference was insignificant as to dermal complaints and Demodex prevalence (p < 0.05), but considerably difference was observed between complaints about eyes and Demodex prevalence (p < 0.05). Consequently, it was concluded that Demodex folliculorum may be a cause of eye disorders such as blepharitis, and this parasite can be frequently observed in persons with immune system anomalies.
