Yazar "Sahin Inan, Zeynep Deniz" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    A digital image analysis study on the disintegration kinetics of reticular fibers in the ethylene glycol-induced rat liver tissue
    (Wiley, 2020) Saraydin, Serpil Unver; Saraydin, Dursun; Sahin Inan, Zeynep Deniz
    Ethylene glycol (EG), the raw material of polyethylenterephthalate, which is the most consumed plastic in the world, has low toxicity, but its metabolites are toxic. EG metabolites can cause acidosis, fibrosis, and eventually cirrhosis in the liver. This study aimed to investigate the effect of EG on rat liver and to determine the quantitative values of the disintegration of reticular fibers (RF) in the liver with the dose duration and to investigate the changes by digital image analysis (DIA). For this purpose, Wistar albino rats were divided into control, and five different daily experimental groups. The control group received saline, and the experimental groups received EG. At the end of experiments, liver tissues of all euthanized rats were removed, and sections were taken, and RF was shown by silver staining. It was observed that the RF fragments in the experimental groups were less than the control group. DIA of RF fragments was then performed with Olympus cellSensDimension 1.15 software and number, area, and ROI% values of the fragments were determined. Statistical analysis revealed that there was a significant difference between control and all experimental groups. RF fragments showed first-order disintegration kinetics, mean disintegration rate constant, and half-time values were 0.1 day(-1)and 7 days, respectively. Consequently, the digital image analysis approach can be a useful tool for the biologist, pathologist, fibrosis-cirrhosis specialist, and computer scientist to understand the effects of toxic chemicals in the liver and analyze reticular fiber disintegration.
  • Küçük Resim Yok
    Öğe
    Evaluation of VEGF, Cytokeratin-19 and Caspase 3 Immunolocalization in the Lung Tissue of Rat with Experimentally Induced Diabetes
    (KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2019) Sahin Inan, Zeynep Deniz; Unver Saraydin, Serpil
    Diabetes Mellitus (DM) manifests itself with changes in the functional structure of the lungs and impairments in gas exchange. These changes in diabetic lung tissue may be due to various factors. Our aim in this study is to correlate the damage of diabetes with lung tissue in terms of VEGF, CK19, caspase 3 immunolocalizations. In this study, animals were divided into 4 groups, 60 mg/kg streptozotocin was given to each of the groups with experimental diabetes and the physiological saline solution was given intraperitoneally to the control group. On days 7 and 14 of the experiment, diabetic and control groups were euthanized, and lung tissues were removed.Tissue samples were evaluated histochemically and immunohistochemically by monitoring with standard light microscopy. In the diabetic group, the localization of CK19 and Caspase 3 increased on the 7th and 14th days compared to t he control group, but the immunolocalization of VEGF decreased. Based on our findings, it was determined that lung tissue was one of the target organs of diabetes.The increase in pulmonary parenchyma due to hyperglycemia is accepted as a source of fibrosis. We concluded that due to increased CK19 localization of fibrosis source, decreased VEGF localization has increased apoptosis in the pulmonary capillary endothelium, which has a significant role in the blood-air barrier in the lung parenchyma, especially in endothelial cells.
  • Küçük Resim Yok
    Öğe
    HER-2 SMASH (vol 95, 10, 2025)
    (Springer, 2025) Alandag, Celal; Ozturk, Aysegul; Yulak, Fatih; Sahin Inan, Zeynep Deniz; Ozkaraca, Mustafa; Lacin, Burak Batuhan; Altun, Ahmet
    [No abstract available]