Yazar "Sen, Metin" seçeneğine göre listele
Listeleniyor 1 - 14 / 14
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antitumoral effects of Allium sivasicum on breast cancer in vitro and in vivo(SPRINGER, 2013) Tepe, Bektas; Tuncer, Ersin; Saraydin, Serpil Unver; Ozer, Hatice; Sen, Metin; Karadayi, Kursat; Inan, Deniz Sahin; Karadayi, Sule; Polat, Zubeyde; Akpulat, Askin; Duman, Mustafa; Koksal, Binnur; Turan, MustafaThis work aims to investigate the antiproliferative properties of Allium sivasicum (AS) on breast cancer. AS extracts were studied for cytotoxicity against the breast cancer cell lines. In vitro apoptosis studies of breast cancer cells were performed by annexin V staining in flow cytometry analyses. AS showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in AS treated cell lines were higher than the untreated control cells. The expressions of caspase-7 protein and TUNEL positive cells were much higher for the rats treated by AS, compared with the untreated control group. The expressions of the Ki-67 decreased in treatment groups compared with the control group. In vivo studies showed that mean tumor volume inhibition ratio in AS treated group was 38 % compared with the untreated rats. These results indicate that A. sivasicum has antitumoral potential against breast cancer.Öğe Antitumoral Effects of Melissa officinalis on Breast Cancer in Vitro and in Vivo(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2012) Saraydin, Serpil Unver; Tuncer, Ersin; Tepe, Bektas; Karadayi, Sule; Ozer, Hatice; Sen, Metin; Karadayi, Kursat; Inan, Deniz; Elagoz, Sahande; Polat, Zubeyde; Duman, Mustafa; Turan, MustafaBackground: There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. Here we investigated the antiproliferative properties of Melissa officinalis (MO) from Turkey on breast cancer. Methods: MO extracts were studied for cytotoxicity against breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). In vitro apoptosis studies were performed by annexin V staining and flow cytometry analyses. Immunohistochemistry for Ki-67 and caspase 7 in the tumoral tissue sections of DMBA-induced mammary tumors in rats was also performed, along with TUNEL assays to detect apoptotic cells. In vivo anticancer activity testing was carried out with reference to inhibition of growth of DMBA induced mammary tumors in rats. Results: MO showed cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells. Expression of caspase-7 protein and TUNEL positive cells were much higher in rats treated by MO, compared with the untreated control group, while expression of Ki-67 was decreased. Furthermore, in vivo studies showed that mean tumor volume inhibition ratio in MO treated group was 40% compared with the untreated rats. Conclusion: These results indicated that MO extrcts have antitumoral potential against breast cancer.Öğe Antitumoral effects of Salvia absconditiflora Greuter & Burdet syn. Salvia cryptantha Montbret & Aucher ex Benth. on Breast cancer(NATL INST SCIENCE COMMUNICATION-NISCAIR, 2013) Ozer, Hatice; Altun, Ahmet; Saraydin, Serpil Unver; Soylu, Sinan; Goktas, Selcuk; Tuncer, Ersin; Inan, Deniz Sahin; Koksal, Binnur; Temiz, Tijen Kaya; Tepe, Bektas; Sen, Metin; Karadayi, Kursat; Turan, MustafaThis work aims to investigate the antiproliferative properties of Salvia cryptantha on breast cancer. Salvia cryptantha (SC) extracts were studied for cytotoxicity against the breast cancer cell lines. In-vitro apoptosis studies of breast cancer cells were performed by nnexin V staining in flow cytometry analyses Immunohistochemistry studies for Ki-67 and p16 in the tumoral tissue sections of Dimethyl Benzanthracene (DMBA) induced mammary tumor in rats were performed. In-vivo anticancer activity testing was carried out by inhibiting the growth of mammary tumor in rats. SC showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in SC treated cell lines were higher than the untreated control cells. The expression of the Ki-67 decreased in treatment groups compared with the control group. The expression of p16 protein was much higher for the rats treated by SC, compared with the untreated control group. In vivo studies showed that mean tumor volume inhibition ratio in SC treated group was 38 % compared with the untreated rats. These results indicate that Salvia cryptantha has antitumoral potential against breast cancer.Öğe Cytoreductive Surgery Followed by Hyperthermic Intraperitoneal Chemotherapy: Morbidity and Mortality Analysis of Our Patients(ORTADOGU AD PRES & PUBL CO, 2012) Karadayi, Kursat; Turan, Mustafa; Karadayi, Sule; Alagozlu, Hakan; Kilickap, Saadettin; Buyukcelik, Abdullah; Sarkis, Cihat; Yucel, Birsen; Boztosun, Abdullah; Cetin, Meral; Yilmaz, Abdulkerim; Yanik, Ali; Sen, MetinObjective: The purpose of this study was to analyze the morbidity and mortality of cytoreductive surgery (CRS) plus hyperthermic intraperitoneal chemotherapy (HIPEC) and early postoperative intraperitoneal chemotherapy (EPIC) with closed abdomen technique in the treatment of peritoneal surface malignancies. Material and Methods: Twenty-six patients (8 with ovarian cancer, 7 peritoneal mesothelioma, 6 colorectal cancer, 3 uterine sarcoma, 1 peritoneal carcinoma and 1 with gastric cancer) underwent 27 procedures. Peritonectomy was performed with complete removal of all the involved visceral and parietal peritoneum. HIPEC was performed with the closed abdominal technique using preheated (42.5 degrees C) perfusate for 60 minutes. EPIC was continued for postoperative 5 days. Results: All patients underwent resection of the lesions. Total pentonectomy was performed in 12 patients, while subtotal or partial peritonectomy was carried out in 14 according to the spread of carcinomatosis. Completeness of cytoreduction score of our patients was 0 in 18 patients, 1 in 6 patients and 2 in 2 patients. Major morbidity developed in 7 patients (27%). CRS+HIPEC+EPIC yielded acceptable morbidity and mortality rates. Of the 26 patients, 20 (77%) were alive without evidence of disease with a mean follow-up period of 13 6 months. Overall 1 year survival was 60%. Conclusion: Cytoreductive approach combined with intraperitoneal chemotherapy prolongs survival in selected patients with peritoneal carcinomatosis (PC) with acceptable morbidity and mortality.Öğe Effect of peritoneal lavage with chlorhexidine gluconate solution on survival and adhesion formation(Turkish Surgical Assoc, 2005) Topcu, Omer; Duman, Mustafa; Koyuncu, Ayhan; Aydin, Cengiz; Turan, Mustafa; Sen, MetinPurpose: In this experimental study; effects of chlorhexidine glucunate solution on peritoneal adhesion formation and perioperative mortality were investigated. Peritoneal Hydatidosis (PH) still remains the most important complication of hydatid cyst surgery. Peritoneal lavage with scolocidal agents and chemotherapy are suggested as a therapeutic approach in the peritoneal dissemination. Materials and Methods: Eighty male rats were divided into four equal groups. Group 1: 0.9% NaCl (Control), Group 2: 20% NaCl, Group 3: 0.4% Chlorhexidine Glucunate (Chx-Glu), and Grup 4: 0.04% Chx-Glu. Two ml solution in each group was instilled into the peritoneal cavity through a median laparotomy and aspirated 5 minutes later. Animals that survived the perioperative period were sacrificed at the fifteenth postoperative day. Adhesion formation were macroscopically graded by Nair criteria. Results: Mortality rate was not significantly different between groups (p > 0.05). Adhesion formation was significantly low in study groups compared to control group (p < 0.05). Adhesion formation was significantly low in Chx-Glu groups compared to 20% NaCl group (p < 0.05). There was no diffirence of adhesion formation between Chx-Glu groups (p > 0.05). Conclusion: Currently, intraabdominal adhesions are still important problems in the surgical area. We recommend the use of 0.04% Chx-Glu solution in the treatment of hydatid disease and Peritoneal Hydatidosis as a potent and non-toxic agent in addition to its ability for decreasing intrabdominal adhesion formation.Öğe The effects of various scolicidal agents on the hepatopancreatic biliary system(KARGER, 2006) Topcu, Omer; Aydin, Cengiz; Arici, Sema; Duman, Mustafa; Koyuncu, Ayhan; Sen, MetinBackground: Sclerosing cholangitis is a rare but dreadful complication of liver hydatid surgery. Almost all scolicidal agents were shown to be potentially associated with this complication clinically or experimentally. This experimental study was designed to examine the effects of 0.04% chlorhexidine gluconate and 95% ethanol on the hepatobiliary system and pancreas and also to compare their effects with 10% povidone iodine and hypertonic 20% saline. Materials and Methods: 50 Wistar rats were divided into equal five groups identified as: 0.9% NaCl ( control group), 0.04% chlorhexidine gluconate, hypertonic 20% saline, 10% povidone iodine, and 95% ethanol. Scolicidal agents were injected into the biliary tract through a duodenal puncture. The animals were kept for 3 months. The animals were sacrificed, and histopathological examinations of the liver, biliary tract, and pancreas were performed. Results: The most severe histopathological changes of the liver, biliary tract, and pancreas were found in the ethanol group, and the least changes were detected in the chlorhexidine gluconate group. Conclusion: This experimental study confirms the deleterious effect of 95% ethanol on hepatopancreatic biliary system when used for the percutaneous drainage of hydatid diseases. For treatment of hydatid cysts in the hepatobiliary system, 0.04% chlorhexidine gluconate should be used as scolicidal agent.Öğe Epigenetic Inactivation of Tumor Suppressor SFRP2 and Point Mutation in KRAS Proto-Oncogene in Fistula - Associated Mucinous Type Anal Adenocarcinoma: Report of Two Cases(JAPAN SOC INTERNAL MEDICINE, 2010) Sen, Metin; Ozdemir, Oztuerk; Turan, Mustafa; Arici, Sema; Yildiz, Fazilet; Koksal, Binnur; Goze, FahrettinThe secreted frizzled-related proteins (SFRPs) genes are unmethylated in normal colorectal mucosa tissue but abberant methylation profiles can be detected in colorectal cancer (CRC), adenomas, and in aberrant crypt foci. The aim of the current study was to clarify whether SFRP2 methylation and K-ras structural mutation in fecal DNA can be found in stool and tumoral tissues of individuals with fistula-associated mucinous type anal adenocarcinomas (MTAA). Two man patients (68 and 56 years old) were treated for anorectal fistula in the surgical department. Patients were evaluated for clinical findings, tumoural tissue samples were examined histopathologically and DNA from fecal and tumoral tissue samples were isolated. K-ras mutation and promoter hypermethylation of SFRP2 gene in tumoral tissues were assessed by methylation-specific PCR based stripAssay hybridisation technique (Me-PCR) and compared to the healthy controls. Fecal and tumoural tissue samples from both patients were found to be fully hypermethylated profiles for SFRP2 gene and combined point mutations were detected in codon 12 and 13 of K-ras proto-oncogene. The current results showed that the combined effects of somatic mutations in K-ras and epigenetic alterations in SFRP2 genes may play an active role in the development of mucinous type anal adenocarcinoma.Öğe Functional Gene Mediated Oncogenesis by Germ-Line MTHFR Polymorphism in Colorectal Carcinoma(Springer, 2013) Ozen, Filiz; Sen, Metin; Ozcirak, Seyma; Colak, Ahmet; Ozdemir, Ozturk[Abstract Not Available]Öğe Homozygous hypermethylation of tumor suppressor SFRP2 gene in a case with mucinous anal adenocarcinoma(SPRINGER, 2009) Sen, Metin; Ozdemir, Oztuerk; Colak, Ahmet; Turan, Mustafa; Arici, Sema; Oezcan, Dogan; Yildirim, Yesim; Koksal, Binnur…Öğe The impact of Ramadan on peptic ulcer perforation(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2012) Gokakin, Ali Kagan; Kurt, Atilla; Atabey, Mustafa; Koyuncu, Ayhan; Topcu, Omer; Aydin, Cengiz; Sen, Metin; Akgol, GunduzBACKGROUND Medical treatment has played an important role in the reduction of peptic ulcer perforation (PUP). The goal of this study was to evaluate the effect of fasting on PUP. METHODS A retrospective analysis of 229 patients who were operated due to PUP between 1999-2009 was made. Patients were divided into two groups. Group I (n=188) included the patients who were operated in other periods of the year, while Group II (n=41) included the patients who were operated during Ramadan, the Muslim period of fasting. Patients in Group II were analyzed in terms of duration of fasting. RESULTS The increase in surgeries per group was higher in Group II than Group I (p<0.05). Predisposing factors, anti-ulcer drug usage and demographic variables were seen to have no role in this difference. Duration of fasting may have a minimal effect on the perforation. CONCLUSION The results of this study demonstrate that PUP is detected as relatively higher during Ramadan among those who are fasting for more than 12 hours daily. We suggest that people with predisposing factors should be informed before making a decision to fast.Öğe Methylenetetrahydrofolate Reductase Gene Germ-Line C677T and A1298C SNPs are Associated with Colorectal Cancer Risk in the Turkish Population(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2014) Ozen, Filiz; Sen, Metin; Ozdemir, OzturkColorectal cancer (CRC) is the third most common cause of death due to cancer in the worldwide and the incidence is also increasing in Turkey. Our present aim was to investigate any association between germ-line methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms and CRC risk in Turkey. A total of 86 CRC cases and 212 control individuals of the same ethnicity were included in the current study. Peripheral blood-DNA samples were used for genotyping by StripAssay technique, based on the reverse-hybridization principle and real-time PCR methods. Results were compared in Pearson Chi-square and multiple logistic regression models. The MTHFR 677TT (homozygous) genotype was found in 20.9% and the T allele frequency 4.2-fold increased in CRC when compared with the control group. The second SNP MTHFR 1298CC (homozygous) genotype was found in 14.0% and the C allele frequency 1.4-fold elevated in the CRC group. The current data suggest strong associations between both SNPs of germ-line MTHFR 677 C>T and 1298 A>C genotypes and CRC susceptibility in the Turkish population. Now the results need to be confirmed with a larger sample size.Öğe New partial splenectomy technique using radiofrequency ablation technology(WILEY, 2010) Karadayi, Kursat; Turan, Mustafa; Sen, MetinThe present case is of a patient undergoing radiofrequency-assisted partial splenectomy. A 48-year-old man was found to have a hydatic cyst in the superior pole of the spleen. A six in-line needle radiofrequency probe was applied to the spleen. The division of the splenic parenchyma was completed with a surgical scalpel through the midline of the ablated tissue. There was no bleeding. This procedure may reduce the number of splenectomies and the amount of blood transfusion required.Öğe A New Technique for Partial Spleenectomy With Radiofrequency Technology(LIPPINCOTT WILLIAMS & WILKINS, 2011) Karadayi, Kursat; Turan, Mustafa; Sen, MetinThe advantage of partial splenectomy is the preservation of its immunologic function. In this series, 8 patients underwent a spleen preservation procedure with radiofrequency. Four of the partial splenectomy procedures were performed in elective situations, whereas the other 4 cases were performed to control traumatic bleeding in emergency situations. A harrow-like radiofrequency probe with 6 needles was applied to the spleen, and the division of the splenic parenchyma was completed using a surgical scalpel through the midline of the ablated tissue. This safe, fast, and simple technique allows for preservation of splenic function with minimum blood loss.Öğe The effects of specific and non-specific phosphodiesterase inhibitors and N-acetylcysteine on oxidative stress and remote organ injury in two-hit trauma model(Turkish Assoc Trauma Emergency Surgery, 2020) Ozer, Ozdemir; Topal, Ugur; Sen, MetinBACKGROUND: Sepsis is a systemic inflammatory response to infection and is one of the leading causes of morbidity and mortality. The second hit after trauma causes increased inflammatory response and multiple organ failure (MOF). The infection which develops after burn injury is a suitable model for a two-hit trauma study. Sepsis causes the release of biochemical mediators, such as Free Oxygen Radicals (FORs), which may lead to lipid peroxidation, which may play a key role in multiple organ failure. In this study, we aimed to investigate the effects of phosphodiesterase (PDE) inhibitors (sildenafil, milrinone, pentoxifylline) and N-acetylcysteine (NAS) on oxidative stress and organ damage in two-hit models. METHODS: In this experimental study, peritonitis was created by cecal ligation and puncture (CLP) method in 40 rats, 72 hours after creating a 30% scalding injury. Rats were divided into five groups of eight rats each as follows: Group I: No treatment; Group II: 10/mg/kg/day dosage of intraperitoneal (i.p) sildenafil treatment was applied for 72 hours after CLP; Group III: 1/mg/kg/day dosage of i.p milrinone treatment was applied for 72 hours after CLP; Group IV: 150/mg/kg/day dosage of i.p NAS treatment was applied for 72 hours after CLP; Group V: 50/mg/kg/day dosage of i.p pentoxifylline treatment was applied for 72 hours after CLP. All rats were sacrificed on the seventh day of this study. Malondialdehyde (MDA), Glutathione Peroxidase (GPx), Superoxide Dismutase (SOD), catalase, Tumor Necrotic Factor-alpha (TNF-alpha) levels, and tissue (lung, kidney) and serum samples were taken for histopathological study. RESULTS: When compared to the control group, the tissue damage score was found to be lower in all treatment groups. Sildenafil, milrinone and NAS groups had higher kidney GPx levels compared to the control group. Milrinone and pentoxifylline were higher in the lung tissue compared to the SOD control group. TNF alpha levels were lower in pentoxifylline and milrinone groups compared to the control group. CONCLUSION: This experimental study has shown that PDE inhibitors and NAS have a decreasing effect on oxidative stress and distant organ damage in the two-hit model. Further clinical and experimental studies are needed on this subject.
