Yazar "Siliğ, Yavuz" seçeneğine göre listele
Listeleniyor 1 - 15 / 15
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Allium Polyanthum Schult Ekstraktı ve Dosetakselin Kolorektal Kanser Hücrelerindeki Apoptozla İlgili Genler Üzerindeki Sinerjik Etkisinin Araştırılması(Sivas Cumhuriyet Üniversitesi, 2024) Tunçbilek, Zuhal; Kabak, Gonca; Siliğ, YavuzAmaç: Kolorektal kanser (CRC), yüksek mortalite ve yaygın metastazlı bir kanser türüdür. CRC'nin erken teşhis edildiği durumlarda kemoterapi ve cerrahi uygulamalar esastır. Docetaxel, taksan grubunda bir antikanser ajandır. Ayrıca, tıbbi aktiviteye sahip bitkilerin CRC üzerinde koruyucu bir etkiye sahip olduğu bilinmektedir. Amaç, Allium polyanthum Schult bitki özütü ve bu bitki özütünün dosetaksel ile kombinasyonu ile tedavi edilen kolorektal kanser hücrelerinde apoptozisle ilişkili genlerin ifade düzeylerini belirlemekti. Gereç ve Yöntem: Sadece Allium polyanthum Schult bitki ekstresi ve bu bitki ekstresiyle birlikte docetaxel uygulanan CRC hücre hattı HT-29 ve sağlıklı kolon hücre hattı CCD-18Co'da apoptozda rol oynayan kaspaz-2 (CASP2), nükleer faktör NF-kappa-B1 (NFKB1), apoptoz düzenleyici BAX ve proto-onkogen MYC genlerinin ekspresyon analizleri, gerçek zamanlı polimeraz zincir reaksiyonu (RT-PCR) yöntemi kullanılarak gerçekleştirildi. Çeşitli veritabanları kullanılarak ilgili genlerin biyoinfarmatik analizi gerçekleştirildi. Sonuçlar: CASP2, MYC, NFKB1 ve BAX gen ekspresyonu, Allium polyanthum Schult özütü ve docetaxel kombinasyonu ile tedavi edilen CRC hücrelerinde sağlıklı hücrelere kıyasla önemli ölçüde azaldı. Biyoenformatik analiz sonucunda, CASP2, MYC, NFKB1 ve BAX proteinlerinin birbirleriyle etkileşime girdiği ve genlerinin ekspresyon seviyelerinin hayatta kalma ile ilişkili olduğu bulundu. Ayrıca, CASP2 ve NFKB1'in metilasyon durumu, CRC'de epigenetik mekanizmaları etkileyerek protein seviyelerini değiştirme potansiyeline sahiptir. Tartışma: Literatürdeki bilgilere göre, Allium türlerinin apoptotik yollardaki genleri etkilediği bildirilmiştir. Buna göre, Allium polyanthum Schult'un tek başına ve docetaksel ile kombinasyonu daha fazla çalışma ile desteklenmelidir.Öğe Bazı nitrozaminlerin diyabetik rat karaciğer mikrozomal sistemi ile oluşturulan denitroasyon ve dealkilasyon tepkimelerinin incelenmesi(Cumhuriyet Üniversitesi, 1997) Siliğ, Yavuz; Çetinkaya, ÖgeÖZET Bu çalışmada, kimyasal olarak diyabet oluşturulan ve diyabet oluşturulduktan sonra etanol, aseton ve izopropanol uygulanan ratların karaciğer mikrozomal sisteminde Nitrozodimetilamin ve N-Nitrozodietilaminin denitrozasyon ve dekilasyon tepkimeleri incelendi. Her iki nitrozaminin de denitrozasyon tepkimesi sonucu oluşan nitrit miktarı tayin edilip nmol/mg mikrozomal protein/dakika olarak verildi. Dealkilasyon tepkimelerinde ise, Nitrozodimetilaminin demetilasyonunda oluşan formaldehit ve N- Nitrozodietilaminin deetilasyonunda oluşan asetaldehit miktarları tayin edilip nmol/mg mikrozomal protein/ dakika olarak verildi. Diyabet ve diyabet sonrası indükleyici verilen ratlarda ; her iki nitrozamini metabolize eden dealkilasyon ve denitrozasyon enzimlerinin Km ve Vmax değerleri bulundu. Her iki nitrozamin için, kontrol grubuna göre diyabetin, diyebetik gruba göre de indükleyici uygulanan enzimlerin indüklendiği görüldü. inhibisyon çalışması sonucunda, nitrozaminlerin aynı oranda metabolize olduğu görüldü. Metabolize eden sistemin her iki nitrozamini (kompetetif olarak inhibe ettiği) metabolize etmek için yarıştığı gözlendi. Diyabet ve diyabet sonrası verilen indükleyicüerin sadece bir tane izoenzimi indüklediği düşüncesi desteklendi.Öğe Biyoinformatik Yaklaşımlar Kullanılarak Glioblastoma Multiform’ da SLC2A Gen Ailesinin Prognostik Potansiyelinin Ortaya Çıkarılması(Sivas Cumhuriyet Üniversitesi, 2024) Taş, Ayça; Siliğ, YavuzAmaç: Glioblastomalar (GBM'ler) çok düşük genel sağkalım oranlarına sahip invaziv ve metastatik kanserlerdir. Bu nedenle, GBM tanısı ve prognozu için yeni bir biyobelirteç önermek çok önemlidir. Bu amaçla, kanserde büyük öneme sahip olan SLC2A gen ailesinin GBM'deki prognostik potansiyelini araştırmayı amaçladık. Yöntemler: Solute taşıyıcı 2A (SLC2A) gen ailesi ekspresyon düzeyleri, metilasyon ve genel sağkalım oranları TCGA, GEPIA ve UALCAN veri tabanları ile analiz edildi. Mutasyonlar Kaplan-Meier Plot ve UCSC Xena veri tabanı ile değerlendirildi. Protein-protein etkileşimleri String veri tabanı ile analiz edildi. Bulgular: SLC2A gen ailesinde istatistiksel olarak anlamlı bir mutasyon saptanmadı. Analiz sonucunda SLC2A1 ve SLC2A5 genlerinde yüksek ekspresyon ve SLC2A6 gen ekspresyonunda azalma istatistiksel olarak anlamlı bulundu. SLC2A1, SLC2A2, SLC2A3 ve SLC2A5 genlerinin promotör bölgelerinde hipermetilasyon, SLC2A4 ve SLC2A6 genlerinde ise hipometilasyon saptandı. SLC2A3 gen ekspresyonundaki artış hastaların genel sağ kalım oranı ile ilişkili bulundu. Sonuç: SLC2A1, SLC2A5 ve SLC2A6 geninin yukarı regülasyonu GBM tanısında bir biyobelirteç olabilir ve SLC2A3 prognozda bir belirteç olabilir.Öğe CLASSIFICATION OF FIBROMYALGIA SYNDROME WITH SUPPORT VECTOR MACHINES(İstanbul Üniversitesi, 2017) Zontul, Cemile; Hayta, Emrullah; Zontul, Metin; Taş, Ayça; Siliğ, YavuzFibromyalgia syndrome (FMS) is a long-term common body pain and a defined chronic pain syndrome. Fibromyalgia syndrome is a difficult disease to diagnose. For this reason, after many unnecessary treatments are applied to the patient, the diagnosis of FMS is usually delayed by clinical examination and evaluation of the patient's complaints. In this respect, there is a need for a decision support system that will facilitate the diagnosis of FMS. In this study, by using the questions asked 351 respondents, 175 FSM patients and 176 healthy control subjects and experimental data, FMS classification was performed with Support Vector Machines which is one of machine learning methods and 85% success was achieved.Öğe Cytotoxic activity of zinc oxide/titanium dioxide nanoparticles on prostate cancer cells(2019) Taş, Ayça; Çakmak, Neşe Keklikcioğlu; Agbektas, Tugba; Siliğ, YavuzProstate cancer is caused by uncontrolled growth of cells in the prostate gland. The aim of this study was to determine the cytotoxic activity of titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles (NPs) and TiO2 + ZnO nanocomposite (NC) in human prostate cancer cell line (DU-145) and healthy mouse fibroblast cell line (L-929). In the study, TiO2 and ZnO NPs and TiO2 + ZnO NC were synthesized. Cytotoxic activities of NC and NPs was then analyzed in human prostate cancer cell line (DU-145) and healthy mouse fibroblast cell lines (L-929) using the MTT method. TiO2, ZnO and TiO2 + ZnO IC50 values were determined in DU-145 and L-929 cell cells (n = 6). TiO2 + ZnO NC in the Du-145 cell line was found as the most active, having statistically significant (***p < 0.0001, **p ? 0.001 and **p ? 0.01).Öğe Determination of the impacts of titanium dioxide nanoparticles on a number of xenobiotic-metabolizing enzymes in rat liver(Burdur Mehmet Akif Ersoy University, 2020) Taş, Ayça; Keklikcioğlu Çakmak, Neşe; Agbektaş, Tuğba; Zontul, Cemile; Özmen, Esma; Siliğ, YavuzNanotechnology techniques are used in many applications, such as cancer treatment, radiological imaging methods, and pharmaceutical industry, as well as in the microbiology field, tissue regeneration, injury healing, treatment of some chronic diseases, and production of vaccines. Whereas products of nanotechnology have a lot of benefits mentioned in our life, they also have some systemic, genetic, and toxic effects in organisms. This study’s goal was to reveal the impacts of titanium dioxide (TiO2) nanoparticles on a number of xenobiotic-metabolizing enzymes in the rat liver fraction. In the current research, adult Wistar albino rats having a weight of approximately 150-200 g and fed under normal conditions were utilized. The incubation of four various concentrations of TiO2 nanoparticles (0.5, 1, 5, and 10 ppm) was performed in the liver fractions. We studied the effects of TiO2 nanoparticles on some enzymes identified in the microsomal fraction, such as N-nitrosodimethylamine demethylase (cytochrome P4502E1), NADPH cytochrome c reductase, NADH cytochrome b5 reductase, and other enzymes found in the cytosolic fraction, e.g. glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PDH), and glutathione level (GSH). GST, G6PDH, NADH-cytochrome b5 reductase, and NADPH cytochrome c reductase levels decreased statistically significantly, whereas the GSH level increased significantly in comparison with controls (p 0.05). Accordingly, in this study, we have shown that TiO2 nanoparticles are capable of inhibiting xenobiotic-metabolizing enzymes. Therefore, this inhibition can affect the detoxification system negatively.Öğe Does dietary intake of acrylamide affect hydroxyproline levels? an animal study(2022) Agbektas, Tugba; Pazarcı, Özhan; Taş, Ayça; Çakmak, Neşe Keklikcioğlu; Kılınç, Seyran; Siliğ, YavuzAcrylamide is a chemical that occurs due to high temperatures during cooking. It consists of an amino acid found in foods and sugars. Studies have shown that cancer formation occurs within the scope of oxidant reagents and DNA damage due to exposure to acrylamide. Our study aims to examine the effects of dietary acrylamide intake on plasma hydroxyproline levels in rats. In this study, 4 groups were formed with 8 rats in each group (total number=32). Blood samples were collected on days 14 and 28. Acrylamide solution was applied to each rat in the treatment group by gastric gavage process at 5 mg/kg three times a week. Hydroxyproline levels in rats’ plasma samples were measured. The median (IQR) hydroxyproline levels were 7.40(2.45) ?g/L in group 1 (14. days control group) and 7.98(3.34) ?g/L in group 2 (14. days acrylamide applied) who received acrylamide. The mean hydroxyproline levels were 7.25(1.96) ?g/L in group 3 (28. days control group) and 9.76(2.64) ?g/L in group 4 (28. days acryl- amide applied) who received acrylamide. No difference was observed between the groups. Dietary acrylamide intake did not have a significant effect on hydroxyproline levels at the application dose and duration in our study.Öğe Investigation of The Effect of B-108 Contaınıng Azomethin Group On DNA Repair Gene(Sivas Cumhuriyet University, 2022) Eğilmez, Elif; Zontul, Cemile; Huseynzada, Alakbar; Aliyeva, Gunel; Hasanova, Ulviyya; Taş, Ayça; Siliğ, YavuzObjective: Cancer arises as a result of the failure of the mechanisms controlling normal division in a group of cells. It is known that some new synthesis compounds intended for use in cancer treatment have anti-fungal, anti-bacterial, anti-carcinogenic effects. In this study, it was aimed to apply the newly synthesized B-108 compound to the A-549 cell line and then to investigate the effect of this compound on the ERCC1 gene expression profile. Materials and Methods: Firstly, compound B-108 was synthesized in our study. Afterwards, this synthesized molecule was applied in eight different concentrations (1-100 μg/ml) in A-549 cell line and 3-(4,5-dimethylthiazol-2-yl)-2,5-yl for 24 hours, 48 hours and 72 hours. Anticancer activities were determined using diphenyltetrazolium bromide (MTT) method. Expression level of DNA repair gene (ERCC1) was determined using RT-PCR method. Results: As a result, it was determined that the molecule applied to the A-549 cell line showed the highest activity after 72 hours of incubation. It was observed that the ERCC1 gene expression of the molecule applied on lung cancer was lower than the control group. Discussion: Considering the current study results, low expression of ERCC1 shows that compound B-108 correlates with overall survival on lung cancer cells.Öğe Investigation of The Effect of Compound B-47/2 Containing Azomethine Group On Angiogenesis(Sivas Cumhuriyet University, 2022) Bucak, Efe Taha; Tunçbilek, Zuhal; Huseynzada, Alakbar; Aghayev, Mirjavid; Hasanova, Ulviyya; Taş, Ayça; Siliğ, YavuzObjective: Lung cancer is one of the most common cancers in the world. It is known that angiogenesis plays a role in the development and metastasis of lung cancer. Azomethine derivatives known as Schiff bases have many biological activities. In this study, we aimed to determine the anticancer activity of the newly synthesized azomethine derivative compound B-47/2 on lung cancer and to determine the effect of this component on vascular endothelial growth factor B (VEGFB) gene expression. Material and Method: Compound B-47/2 was synthesized for the first time. B-47/2 compound was applied to lung cancer cell line (A549) at varying concentrations (1-100 µg/mL) and its anticancer activity was found after 24, 48 and 72 hours incubations using the 3-Öğe Investigation of the relationship between ?2- adrenergic receptor (?2-AR) polymorphism and gastric cancer(2018) Atabey, Mustafa; Taş, Ayça; Ağbektaş, Tuğba; Bostancı, Meriç Emre; Topçu, Ömer; Siliğ, YavuzObjective: Gastric cancer is a multifunctional disease. Emotional stress, physiological and neuroendocrine changes incancer patients can lead to the activation of the hypothalamic-pituitary-adrenal axis and the release of hormonedependent hormones such as catecholamine. In particular, it has been reported that catecholamine induction directlyaffects the biological behavior of tumor cells via ?2-Adrenergic receptor (?2-AR) mediated signaling. In this study, itwas aimed to investigate polymorphism of ?2-AR gene (rs1042717) in gastric cancer patients.Method: Polymorphism in the ?2-AR gene (rs1042717) was determined by Real-Time PCR method in 60 gastric cancerpatients and 50 healthy controls. The results were evaluated using logistic regression and Chi-square (?2) test.Results: The comparison of gastric cancer patients and controls determined a statistically significant relationship foralcoholic drink consumption (p<0,05). There was a statistically significant relationship between ?2-AR (rs1042717)polymorphism and stomach cancer (p <0.05). There was a statistically significant relationship between mutant (AA)genotype with wild type (GG) and heterozygous (AG) polymorphic genotypes when evaluated in ?2-AR polymorphismgastric cancer patients and control group (?2: 19,38, p: 0.001). Similarly, there was a statistically significant correlationbetween heterozygous (AG) with wild type (GG) and mutant (AA) polymorphic genotypes in gastric cancer (?2: 14, 27,p: 0,001).Conclusions: In this study, it was found that the AG genotype is predominant in gastric cancer patients and controls,and that the AA genotype has a protective effect against gastric cancer.Öğe Investigation of the relationship between exportin5 (XPO5) polymorphism and gastric cancer(Sivas Cumhuriyet University, 2021) Tufa, Abdisa; Taş, Ayça; Ağbektaş, Tuğba; Topcu, Ömer; Genet, Solomon; Siliğ, YavuzObjective: We aimed to analyzed the association between gastric cancer and polymorphism of XPO5 gene.Method: The polymorphism in the XPO5 gene (rs11544382) was determined in 120 individuals (60 gastric cancer patients; 60 healthy controls) using Real-Time PCR method.Results: The comparison of gastric cancer patients and controls revealed a statistically significant relationship for alcoholic drink consumption (p<0.05).The relationship between XPO5 gene (rs11544382) polymorphism and gastric cancer was statistically not significant. There was no a statistically significant relationship between mutant (GG) genotype with both wild type (AA) and heterozygous (AG) polymorphic genotypes when evaluated in XPO5 polymorphism gastric cancer patients and control groups (χ2:0.12, p=0.729). The heterozygous (AG) was dominant in gastric cancers patients and control subjects, 93.3 and 91.7% respectively.Conclusions: This study provides information about alleleand genotype frequency distribution of XPO5 gene polymorphism (rs11544382) in Turkish At the same time, AG genotype was found to be dominant in gastric cancer patients and their controls.Öğe L-Valin amino asitinin insan eritrosit membranından taşınımına dietilnitrozaminin etkisi(Cumhuriyet Üniversitesi, 1990) Siliğ, Yavuz; Aker, Ahmet6. ÖZET Bu çalışmada mutajenik ve karsinojenik etkileri olduğu bilinen dietilnitrozaminin insan eritrosit membranmda L-Valin amino asiti taşmımma etkisi in celendi. înkübasyon süresi için yapılan deneyler sonu cunda 20 dk seçildi. 50 o dk üzerinde hemoliz olayın dan dolayı hücre içine taşınan L-Valin miktarında azalma olduğu gözlendi. Kullanılan kan numunelerinde hemotokrit değeri arttıkça taşımının arttığı bulundu. Bütün deneyler % 20 hematokrit değerli kanlar kulla nılarak standart bir ortam sağlandı. DEHA deriş iminin amino asit taşmımma etkisi ni belirlemek için yapılan çalışmalarda DEHA'nın de rişimi arttıkça hücre içine taşınan L-Valin miktarı nın kontrollere kıyasla azaldığı başka bir deyimle DEHA'nın L-Valin' in hücre içine taş mimini inhibe ettiği saptandı. Bu inhibisyonun, L-Valin 'in DEHA ile bağlanmasıyla değil, DEHA'nın taşıyıcı sistemdeki proteini alkillemesiyle oluşabileceği gösterildi.Öğe Mir146a Polymorphism in Gastric, Colon and Rectum Cancers(Sivas Cumhuriyet Üniversitesi, 2023) Ağbektaş, Tuğba; Taş, Ayça; Atabey, Mustafa; Sarı, İsmail; Bostancı, Meriç Emre; Topcu, Ömer; Siliğ, Yavuzmir146a, a member of the microRNA family, plays an important role in the regulation of many biological pathways such as the regulation and differentiation of hematopoietic cells. The relationship between mir146a polymorphism and gastric, colon, rectum cancers have been investigated in Turkish population. Polymorphism in mir146a gene rs2961920 and rs2910164 have been determined in 212 patients (gastric: 73, colon:76 and rectum:63) and in 77 healthy controls by Real-Time PCR. Findings were evaluated by logistic regression and Khi (χ2) tests. The comparison of gastric, colon and rectum cancer patients and controls determined a statistically significant relationship for alcoholic drink consumption (p0.05). However, there was statistically significant relationship between this polymorphism and gastric cancer in GG+CG and CC genotypes when the gastric cancer patients and control group were evaluated for mir146a rs2910164 polymorphism (χ2: 5,49 p: 0,019). Similarly, there was statistically significant relationship between this polymorphism and gastric cancer in GG+CC and CG genotypes (χ2:5,39, p: 0,020). In this study, it is thought that by investigating the functions of microRNAs and their role in cancer, it may be promising in understanding the molecular pathology of cancer and in developing molecular targeted therapies.Öğe Potassium Ion Channel Protein (KCNH) Levels in Patients with Fibromyalgia Syndrome(CMB Association Publiher, 2021) Taş, Ayça; Hayta, Emrullah; Karadağ, Ahmet; Zontul, Cemile; Özmen, Esma; Aydın, Süleyman; Siliğ, YavuzAlthough there is not yet full clarity of the pathogenesis of fibromyalgia syndrome (FM), central sensitization is considered to be responsible. The purpose of this study was to measure the plasma levels of potassium ion channel proteins (human KCNH2, KCNH6 and KCNH7) in FM patients and healthy control subjects. The study sample includes 76 newly diagnosed FM patients and 79 healthy individuals. Venous blood samples were taken to measure the plasma levels of KCNH2, KCNH6 and KCNH7. Pain severity in FM patients was assessed using a visual analog scale (VAS). Bioinformatics analysis was performed using the STRING v 11 Protein interaction tool. Age, gender and body mass index were seen to be similar in both groups. In comparisons between FM and control groups, KCNH2 plasma levels was found to be significantly lower in the FM group. No significant correlation was found between plasma levels of KCNH2, KCNH6 and KCNH7 protein levels and VAS score of patients with FM. The KCNH2 protein had a high homology score with 9 proteins. The plasma levels of KCNH2 FM patients were found to be lower than those of the healthy control subjects, no difference was determined in respect of the plasma levels of KCNH6 and KCNH7. These results may be of use in guiding future studies on the pathogenesis of FM.Öğe The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model(İstanbul Üniversitesi, 2022) Bostancı, Meriç Emre; Avcı, Onur; Taş, Ayça; Koç, Tülay; Gürsoy, Sinan; Siliğ, YavuzObjective: The aim of this study was to examine the effect of rectal ozone on the histopathological healing of the colonic mucosa, tissue oxidative stress, and bacterial translocation in the experimental colitis model. Materials and Methods: Three groups of rats were randomly formed
