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    Effects of Metformin on TNF- Release in Lipopolysaccharide-Induced Monocytes in Rats
    (Sivas Cumhuriyet Üniversitesi, 2024) Gedikli, Emre; Parlak, Mesut; Soydan, Ahmet Serdar
    Objective: Inflammation which is a response of immune system was demonstrated in many disorders such as atherosclerosis, hypertension, diabetes, cancer and rheumatoid arthritis. Metformin, an oral antidiabetic drug, has anti-inflammatory effect apart from blood glucose regulatory effect. However, the mechanism of its anti-inflammatory effect is not clearly understood. In this study, the effect of metformin on the release of cytokines (TNF-α and IL-6) from LPS stimulated rat mononuclear blood cells was investigated. Methods: Blood samples (5ml) were taken from healthy, male, 8-12 weeks old rats (n=5, 200-250g) through cardiac puncture under general anesthesia into sterile EDTA containing tubes. Monocytes were separated by centrifugation and were resuspended in RPMI 1640 media (3.3±0.2x105 /ml). Cells were then incubated with metformin (2.5 μM, 25 μM, 250 μM) for 2,5 hours followed by addition of LPS (100 ng/ml, 1μg/ml) for further 5 hours. After centrifugation, the supernatant was taken and TNF-α level was measured by ELISA. Results: There was no statistically significant change in the amounts of TNF-α in the LPS + metformin groups compared to the 100 ng/ml LPS group (p>0.05). In LPS+metformin groups, compared to 1 μg/ml LPS, 2.5 μM and 250 μM metformin significantly increased TNF-α levels (p0.05). The amount of IL-6 was not within measurable range in this study. Conclusion: In summary, metformin increased the amount of released TNF-α rather than decreased in our study.