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Akalküloz akut kolesistit modelinde l-arginin ve aminoguanidin tedavisinin safra kesesi inflamasyonu ve kontraktilitesi üzerine etkileri
(Cumhuriyet Üniversitesi, 2007) Soylu, Sinan; Aydın, Cengiz
Akut akalküloz kolesistit safra kesesi taşı olmadan akut safra kesesi inflamasyonunu içeren klinik bir durumdur ve klinik olarak ateş, lökositoz ve sağ üst kadran ağrısıyla karakterizedir. Bu hastalığın patogenezi tam olarak anlaşılamamıştır. Bilier staz ile olan bozulmuş kas kontaktilitesi ve/veya safra kesesinin inflamasyonu ile sonuçlanan non?oklüziv iskemisi patojenik faktörler olarak ileri sürülmektedir. Ana safra kanalı ligasyonu (ASKL) safra kesesi distansiyonu yapan akalküloz kolesistitin hayvan modelidir ve akut inflamasyon insanlardaki akut akalküloz kolesistit ile benzerdir. ASKL ile akut safra kesesi inflamasyonunun safra kesesi kontraktilitesine etkisi, azalmış düz kas kontraktilitesi ve sinir ile uyarılan kontraksiyonda azalma ile olur. Guinea pigdeki kas kontraktilitesi ASKL + SF, ASKL + Aminoguanidin(AG), ASKL + Aninoguanidin + L-arginin(AG + L-ARG) ve opere sham gruplarında in vitro olarak çalışıldı. KCl, karbakol, histamin ve elektrik alan uyarımı (EAU) safra kesesi kas şeritlerini kasmak için kullanıldı. Hematoxylin eozin boyama ile kas şeritlerinin inflamasyon skorları tespit edildi. Çalışmamızda karbakol, histamine ve EAU'ya bağlı kasılma cevaplarında, akut kolesistit grubunda kontrol grubuna göre azalma olduğu görüldü. AG tedavisi verilen grubun diğer iki kolesistit gruplarına göre kasılma cevabında iyileşme olduğu görüldü(p<0,05). AG + L-ARG tedavisi verilen grubun kasılma değerlerinde akut kolesistit grubuna göre düzelme olduğu görüldü, ancak aralarındaki fark istatiksel olarak anlamlı bulunmadı(p>0,05). AG ile 24 saatlik tedavinin SF grubuna göre inflamasyon skorunu arttırdığı görüldü(p<0,05). Ana safra kanalı ligasyonu sonrası SF, AG ve AG + L-ARG ile 48 saatlik tedavide inflamasyon skorları birbirine yakın olarak bulundu (p>0,05). Bu bulgular ışığında, AG ve AG + L-ARG ile tedavinin akut kolesistitte görülen inflamasyonu iyileştirici etkilerinin olmadığı, AG'in akut kolesistitte kas kasılma cevaplarını düzelttiği sonucu elde edilmiştir. Bu düzelme inflamasyondan bağımsız olup NO sisteminin diğer etkilerinden kaynaklanmış olabilir. Anahtar kelimeler: Akut akalküloz kolesistit, Aminoguanidin, L-arginin, Safra kesesi kontraksiyonu, Safra kesesi inflamasyonu.
Amniotic Membrane-Coated Polypropylene Mesh for the Repair of Incisional Hernia: An Experimental Study In a Rat Model of Abdominal Wall Defect
(KOWSAR PUBL, 2018) Soylu, Sinan; Yildiz, Caglar; Bozkurt, Birkan; Karakus, Savas; Kurt, Begum; Kurt, Atilla
Background: Incisional hernia repairs are among common abdominal wall surgeries, can be primarily required or being reconstructed using a synthetic or biological material. Objectives: This study aimed at evaluating intra-abdominal adhesions and incisional site healing after the repair of abdominal wall by fresh amniotic membrane-coated polypropylene mesh in comparison to only polypropylene mesh in an experimental rat study. Methods: The study protocol was approved by Cumhuriyet University Institutional Ethics Committee for Animal Experiments (Sivas-Turkiye, date 24/06/2015). Sixteen pregnant female Wistar-Albino rats (mean weight, 275 g) were anesthetized on the 21st day of pregnancy and a 1-cm area of the abdominal wall was excised. The pregnancy was terminated, emerging amniotic membranes were dissected, and eight pieces of the 1-cm(2) polypropylene mesh were coated with these amniotic membranes without using any suture or adhesive. The polypropylene meshes were sutured on the abdominal wall of eight rats (control group), selected by simple random sampling. For the remaining eight rats, the same procedure was applied with the amniotic membrane-coated polypropylene meshes (experimental group). On the 28th postoperative day, the anterior abdominal wall was opened, and intra-abdominal adhesions were assessed macroscopically by Nair's adhesion scoring system. Strip-shaped biopsy samples were taken from incision lines for histopathological examination. Results: The experimental group had significantly less intra-abdominal adhesions (i.e. Nair's score of 2 to 4) compared to the control group (two and six rats, respectively; P= 0.046), and had significantly lower mean score for polymorphonuclear leukocyte infiltration (P = 0.039), hyperemia (P= 0.039), and epithelialization (P= 0.039). The score for increase in connective tissue (P = 0.018) was significantly higher in the experimental group, and the scores for edema (P= 0.590) and macrophage infiltration (P= 0.590) were similar between the two groups. Conclusions: The use of polypropylene mesh coated with fresh amniotic membrane provides advantage of decreasing postoperative intra-abdominal adhesions along with less inflammation and higher epithelialization after abdominal wall repair surgery.
Antitumoral effects of Salvia absconditiflora Greuter & Burdet syn. Salvia cryptantha Montbret & Aucher ex Benth. on Breast cancer
(NATL INST SCIENCE COMMUNICATION-NISCAIR, 2013) Ozer, Hatice; Altun, Ahmet; Saraydin, Serpil Unver; Soylu, Sinan; Goktas, Selcuk; Tuncer, Ersin; Inan, Deniz Sahin; Koksal, Binnur; Temiz, Tijen Kaya; Tepe, Bektas; Sen, Metin; Karadayi, Kursat; Turan, Mustafa
This work aims to investigate the antiproliferative properties of Salvia cryptantha on breast cancer. Salvia cryptantha (SC) extracts were studied for cytotoxicity against the breast cancer cell lines. In-vitro apoptosis studies of breast cancer cells were performed by nnexin V staining in flow cytometry analyses Immunohistochemistry studies for Ki-67 and p16 in the tumoral tissue sections of Dimethyl Benzanthracene (DMBA) induced mammary tumor in rats were performed. In-vivo anticancer activity testing was carried out by inhibiting the growth of mammary tumor in rats. SC showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in SC treated cell lines were higher than the untreated control cells. The expression of the Ki-67 decreased in treatment groups compared with the control group. The expression of p16 protein was much higher for the rats treated by SC, compared with the untreated control group. In vivo studies showed that mean tumor volume inhibition ratio in SC treated group was 38 % compared with the untreated rats. These results indicate that Salvia cryptantha has antitumoral potential against breast cancer.
Are using neutrophil‐to‐lymphocyte ratio and platelet parameters useful in predicting Short Bowel Syndrome in patients with mesenteric ischemia?
(Sivas Cumhuriyet University, 2021) Koç, Süleyman; Soylu, Sinan
Objective: Acute mesenteric ischemia (AMI) is a potentially life-threatening medical condition that results in bowel infarction and gangrene. It usually occurs over the age of 60 and is seen in both sexes at similar rates. Early diagnosis and treatment are the cornerstones in the treatment of AMI. Although different biochemical markers for AMI are used clinically, no specific laboratory test has been proven to identify the presence of ischemic or necrotic bowel. In this study, we aimed to evaluate Neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLT/LYM), NLR/PLT, mean platelet volume-PLT ratio (MPV)/PLT, and MPV*NLR/PLT could be a useful tool for early prediction of the severity of AMI.Method: We analyzed retrospectively records of 105 patients diagnosed with acute AMI who were operated on at our clinic. We investigated patient demographics, clinical symptoms, concomitant diseases, blood test results, and data regarding the surgical procedure performed and the resection's width. The patients were divided into two groups as short bowel syndrome (SBS) developing and non-developing.Results: We enrolled a total of 105 AMI patients in this study. There was no significant difference between the rates of non-SBS and SBS in terms of age, gender, diabetes mellitus, and smoking status. WBC, NEU, LYM, and MPV were higher, and PLT was lower in the SBS group. There were no statistically significant differences in MPV/PLT between the two groups. The combined values of NLR, PLT, and MPV were also compared between non-SBS and SBS groups. According to our results, the means of NLR, PLT/LYM, NLR/PLT, MPV/NLR, and MPV*NLR/PLT variables were statistically significantly different between the two groups.Conclusions: Our findings showed that NLR, NLR/PLT, MPV/PLT, MPV*NLR, and MPV*NLR/PLT could be a relatively better tool for early prediction of the severity of AMI and SBS. Further studies have to be carried out to investigate the best parameter for predicting the severity or prognosis of AMI.
Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)
(Sivas Cumhuriyet University, 2021) Genç, Hüsnü; Soylu, Sinan; İnan, Deniz Şahin; Kurt, Atilla; Çoşkun, Hakkı; Yıldırır, Ali
Objective: The basic aim in the treatment of second-degree burns is to prevent progressive cell death, and so treatments are directed at the zone of stasis. In this study, We were aimed to investigate the healing effects of using Mesenchymal Stem Cells (MSCs), Ovarian follicular fluid (OFS) and Platelet-rich plasma (PRP) on burn stasis zone in an experimental burn model.Method: Forty rats were divided randomly into four groups. Burns were created in each group according to the comb burn model. The control group received no treatment; the mesenchymal stem cell
Crocus Officinalis (L.) Extract on Human Colerectal Cancer Cell Line (HT-22): Investigation in Vitro
(2024) Soylu, Sinan
Crocus sativus L. has been used as spice, food colouring and medicinal plant for thousands of years. In this study, antioxidant properties and cytotoxic effect of saffron extract were evaluated in HT-22 cell lines. Malignant and non-malignant cells (L929) were cultured in DMEM medium and incubated with different concentrations of saffron extract in different solvents (water, ethanol, ethyl-acetate, n-hexane). Cell viability was determined by MTT assay. Antioxidant parameters such as DPPH, FRAP, total polyphenol and flavonoid amounts were measured. The highest DPPH value was found as 15.90±0.12 µg/mL in ethyl acetate solvent. Total polyphenols were 89.06±0.02 mg GAE/g extract, flavonoids were 76.98±2.08 µg QE/g extract and FRAP was 67.93±0.98 mg/g extract in ethanol solvent. In colon cancer, IC50 value 94±1.37 µg/mL was found to be the highest value. This is thought to be due to the high antioxidant capacity in ethanol solvent. While antioxidant capacity was not found to be high, antiproliferation effect was found to be high. Saffron may also be considered as a promising chemotherapeutic agent in cancer treatment in the future.
Distinctive Histopathological Features of Fasciola Hepatica Adult
(2024) Kartal, Sümeyra; Aslan, Sema; Öz, Murtaza; Soylu, Sinan; Özer, Hatice
Fasciola hepatica is a parasite of the Trematoda class, found worldwide but primarily seen in cattle-raising countries. It usually settles in the biliary tract and causes nonspecific gastrointestinal symptoms such as abdominal pain, right upper quadrant tend erness, and fever in humans. Parasitological examination of stool or bile samples is required for definitive diagnosis. In our case, demonstrative histopathological features of Fasciola hepatica adult parasite seen in a 38-year-old female patient suffering from abdominal pain were evaluated. Fasciola hepat- ica, a rare parasite in pathology practice, should be considered in the diagnosis in the presence of nonspecific gastrointestinalcomplaints because it can be seen worldwide as well as in endemic regions.
Effect of Strontium Ranelate on Multiple Organ Damage in a Rat Sepsis Model
(KOWSAR PUBL, 2018) Soylu, Sinan; Inan, Zeynep Deniz Sahin
Background: Sepsis and multiple organ dysfunction syndrome (MODS) are life-threatening conditions common in intensive care units. In this regard, studies have shown that Strontium ranelate has anti-inflammatory activity by blocking tumor necrosis factor-alpha (TNF alpha). Objectives: This study aimed to investigate the effect of Strontium ranelate on MODS in an experimental sepsis model. Methods: The study protocol was approved by Cumhuriyet University Institutional Ethics Committee for Animal Experiments (Sivas-Turkiye, date 07/12/2017). Twenty female Wistar-Albino rats were randomly divided into four groups of sham operation, cecal ligation and perforation (CLP), CLP + Strontium (S) (oral 40 mg/kg for 7 days), and S (40 mg/kg oral preoperative 5 days) + CLP + S (oral 40 mg/kg for 7 days). Blood samples were taken, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine were studied. Tissues were removed, and inflammation scores were determined. Results: The mean inflammation scores of lung, liver, and renal tissues were found to be the lowest in the sham group (0.8 +/- 0.45), and they increased in the S + CLP + S (1.6 +/- 0.55) and CLP + S (3.4 +/- 0.55) groups, respectively, with the highest score in the CLP (3.8 +/- 0.45) group. It was found that there was no statistical difference between the sham and S + CLP + S groups (P > 0.05); however, there was a significant difference between the other groups (P < 0.05). The mean ALT, AST, BUN and creatinine values were found to be the lowest in the sham group, and they increased in the S + CLP + S and CLP + S groups, respectively, with the highest score in the CLP group. Regarding the mean ALT results, it was noted that there was no significant difference between the sham and S + CLP + S groups (P > 0.05); however, there was a significant difference between the other groups in terms of mean ALT, and there was a significant difference between all the groups in terms of mean AST, BUN, and creatinine (P < 0.05). Conclusions: It was concluded that Strontium ranelate reduced the development of life-threatening MODS in patients with sepsis, especially when it was administered before the development of sepsis, by suppressing inflammatory mediators.
Effect of the wrapping of experimentally induced ischemic colonic anastomoses with the amniotic membrane on anastomosis safety in rats.
(2018) Soylu, Sinan; İnan, Zeynep Deniz Şahin
Objective: Colonic anastomotic leakage represents a complication with high mortality and morbidity. In the presentstudy, it was aimed to examine the effect of the amniotic membrane on the healing of ischemic colonic anastomosis.Method: 25 pregnant Wistar albino rats aged 19-21 days were randomly divided into 5 groups. With midlinelaparotomy, pregnancies were terminated, and fresh amniotic membranes were obtained. In Group 1 Sham (S), noprocedure except for laparotomy was performed. In Group 2 of colonic anastomosis (A), the descending colon wascompletely transected and anastomosed. In Group 3 of amniotic anastomosis (AA), the anastomosis line was fixed bybeing wrapped with the fresh amniotic membrane. In Group 4 of colonic ischemic anastomosis (IA), the marginalarteries were ligated after descending colon transection. Ischemia was induced, and anastomosis was performed. InGroup 5 of colonic ischemic anastomosis wrapped with the amniotic membrane (AIA), after ischemic colonicanastomosis was performed, the anastomosis line was fixed by being wrapped with the amniotic membrane. On thepostoperative 7th day, rats were sacrificed, and their anastomotic bursting pressures were measured. For histologicalexamination, the colon segment containing the anastomosis line was removed. Hematoxylin-eosin and Van Giesontrichrome stained specimens were histologically evaluated and scored according to the Ehrlich-Hunt model.Results: Anastomotic bursting pressures were measured in group S as the highest, in AA, AIA, A in a decreasing orderand in group IA as the lowest. The difference between groups S and AA was statistically insignificant in the pairedcomparison of groups according to their bursting pressure values (p=0.222). When the other pairs were compared, thedifference between the groups was calculated as significant (p< 0.05). According to the Ehrlich-Hunt model, the totalscore was the highest in AIA group, decreased in AA, IA, A groups in a decreasing order, and was the lowest in groupS. A paired comparison for total scores between the groups was statistically insignificant between groups S and A(p=0.222). Other paired comparisons were statistically significant (p< 0.05).Conclusions: Wrapping the colon anastomosis line with the amniotic membrane gave positive results both in theischemic colon as well as in the non-ischemic colon in terms of bursting pressure and histological anastomosis safety.
Effects of NO/L-Arginine Pathway on Gallbladder Contractility in Bile Duct Ligated Guinea Pigs
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2009) Soylu, Sinan; Aydin, Cengiz; Bagcivan, Ihsan; Yildirim, Sahin; Koyuncu, Ayhan; Topcu, Omer; Arici, Sema
Background. Common bile duct ligation (CBDL) produces gallbladder distension and acute inflammation similar to that seen in human acute acalculous cholecystitis. CBDL in the guinea pig affects smooth muscle contractility. The aim of this study was to determine whether the nitric oxide-L-arginine pathway plays a role in the inflammatory process and abnormal gallbladder contractility that occur after CBDL. Materials and Methods. Contractility of gallbladder muscle from CBDL and sham-operated guinea pigs was studied in vitro. Animals were treated with saline, aminoguanidine (AG), or an aminoguanidine + L-arginine combination (AG + L-Arg) in vivo. Potassium chloride, carbachol, and electric field stimulation (EFS) were used for contracting the gallbladder muscle strips or activating intrinsic nerves. Hematoxylin and eosinstained slides of muscle strips were scored for inflammation. Results. Contraction responses to carbachol and EFS were decreased significantly in CBDL guinea pigs compared with those in the sham-operated group. AG partly reversed the smooth muscle contractile response to carbachol and EFS, but did not reduce the inflammation score. Treatment with AG + L-arg did not reverse either the contraction response or the inflammation score. Conclusions. These findings suggest that AG and AG + L-Arg treatments have no beneficial effect on inflammation in guinea pigs after CBDL, although AG significantly reversed the effect on muscle contractility (P < 0.05). This improvement was independent of inflammation and may be due to a decreased level of NO and its diminished relaxant effect. (C) 2009 Elsevier Inc. All rights reserved.
Effects of strontium ranilate and Hypericum perforatum extract on experimental colitis model in rats
(2018) Kurt, Atilla; Soylu, Sinan; İnal, Zeynep Deniz Şahin; Topçu, Ömer; Atabey, Mustafa
Objective: In this study, we aimed to compare the healing effect of strontium ranilate(SR) and hypericum perfaratum(HPE) with the healing effect of prednisolone used in the treatment of experimental colit in rats.Method: 30 wistar albino rats were used in the study. Rats were randomly assigned to 5 consecutive groups consistingof 5 rats. Group 1, healthy rats, group 2 colitis but untreated rats. The rats in groups 3, 4, and 5 had colitis and received160 mg / kg SR, 1mg/kg prednisolone and HP extracts containing 0.3% hiperisine (250 uq / 300 mg) by oral gavage,respectively for seven days. At the end of the seventh day, the colons of the rats were removed and histopathologicalexamination was performed.Results: The histopathological results of the HPE group were statistically worst including the control group. It wasobserved that the best results in the treatment groups were SR group followed by prednisolone group,respectively.(P<0.05)Conclusions: This study concluded that SR was at least as effective as prednisolone in colitis treatment, but HPEexacerbate colitis-induced inflammation when received by orally.
The effects of wireless electromagnetic fields on the activities of carbonic anhydrase and acetylcholinesterase enzymes in various tissues of rats
(WILEY, 2018) Kocyigit, Umit Muhammet; Taslimi, Parham; Gurses, Fatih; Soylu, Sinan; Dastan, Sevgi Durna; Gulcin, Ilhami
The purpose of our study is to assist in understanding the effects of wireless electromagnetic waves on carbonic anhydrase (CA) and acetylcholinesterase (AChE) enzymes activities in the different tissues of the rats. For this purpose, two different groups each of which contains eight rats (n=8) were formed as being control group and wireless electromagnetic wave-administered group. The rats were necropsied after 60min from the injection of chemicals into the rats intraperitoneally. The different tissues of the rats were extracted. CA and AChE enzymes activities were measured for each tissue. All the experimental results were provided in mean +/- S.D. Statistical significance was identified to be P<0.05. It was observed that there were significant changes of enzyme activities in wireless-administered group in salivary gland, stomach, colon, liver, and striated muscle tissues.
Gastric Diverticulum On The Greater Curvature: a Case Report
(Acıbadem Mehmet Ali Aydınlar Üniversitesi, 2016) Mollaoğlu, Murat Can; Bozkurt, Birkan; Soylu, Sinan; Bostancı, Meriç Emre; Karadayı, Kürşat
Gastric diverticulum is a rare pathological condition of the gastrointestinal system. It is seen in approximately 0.01% of all endoscopic examinations. Gastric diverticulum is commonly asymptomatic and usually detected incidentally during upper endoscopic examination. Gastric diverticulum is often located on the posteromedial wall of the fundus. Rarely, upper abdominal discomfort and pain may be presented. In this case, we report a 57 years old female patient with a gastric diverticulum on the greater curvature
Güvenli Kolesistektomi Gerçekten Güvenli mi?
(Sivas Cumhuriyet Üniversitesi, 2023) Soylu, Sinan
Amaç: Bu çalışma, klinik pratikte safra yolu yaralanmalarının önlenmesinde 'Eleştirel Güvenlik Görüşü' (CVS) tekniğinin etkinliğini göstermek için tek bir cerrahın deneyiminin sonuçlarını sunmayı amaçlamaktadır. Yöntem: 2018-2023 yılları arasında Sivas Cumhuriyet Üniversitesi Hastanesi Genel Cerrahi Anabilim Dalı'nda tek cerrah tarafından CVS tekniği kullanılarak kolesistektomi yapılan 899 hastanın kayıtları retrospektif olarak incelendi. Çalışma için etik onay alındı. Kolesistektomi olguları geriye dönük olarak tarandı. Hastaların yaşı, cinsiyeti, dren yerleşimi, ameliyat endikasyonları ve açık kolesistektomiye geçiş nedenleri kaydedildi. Bulgular: İncelenen 899 hastanın 312'si (%34,70) erkek, 587'si (%65,30) kadındı. Erkeklerde yaş ortalaması 55,10, kadınlarda ise 51,65’ idi. Olguların 7'sinin (%0,77) açık kolesistektomiye dönüştüğü belirlendi. Kolesistektominin en yaygın endikasyonu safra kesesi taşının elektif olarak çıkarılmasıydı ve vakaların %47,05'ini oluşturuyordu. Majör safra kanalı yaralanması tespit edilmedi. Sonuç: Laparoskopik kolesistektomide safra yolu yaralanmalarını önlemek için güvenli kolesistektomi tekniği güvenle uygulanabilir.
Investigation of gene expression levels in thyroid tissues of rats treated with Wi-Fi electromagnetic wave (2.4-3 GHz Wi-Fi RF-EMF)
(Elsevier, 2023) Serin, Musa; Soylu, Sinan; Dastan, Sevgi Durna; Koc, Suleyman; Kurt, Atilla
In this study, it was aimed to examine the effects of wireless waves applied to rats in the thyroid tissue by evaluating gene expression levels on 12 determined gene regions. 20 healthy 16-week-old Wistar albino female rats weighting 200-220 g were used. In the experiments, two groups, control and experimental groups, were formed, and ten female rats were used in each group. While WI-FI electromagnetic field was applied to the experimental group of these rats, nothing was applied to the control group. Many different gene regions, including beta catenin, beta-actin, GAPDH, L3B, HIF 1-Alpha, GSk-3B, TCF, WNT7a, WNT10a, WNT2, Beclin 1 and Beclin 2, ATG5 and ATG12, were investigated. ACTB and GAPDH gene primers were used as a house keeping gene. The fold change values were calculated statistically from the data obtained from the gene expression re-sults. Compared to the control group, it was observed that the experimental group had significant increases in gene expression levels of many gene regions investigated in the study (P<0.05). Among the study groups, 12 different genes such as beta catenin, beta-actin, GAPDH, L3B, HIF 1-Alpha, GSk-3B, TCF, WNT7a, WNT10a, WNT2, Beclin 1 and Beclin 2, ATG5 and ATG12, which we have determined according to the literature, are known to be related to autophagy and oxidative stress. When the expression levels were investigated, it was determined that the expression coefficients of all the genes studied in the wnt/ss catenin pathway in the experimental group of rats exposed to the Wi-Fi EM field, except for ATG5 and ATG12, were quite high, and there was significant differences between the groups.
Lazer Hemoroidopeksi Klinik Deneyimimiz
(Sivas Cumhuriyet Üniversitesi, 2024) Genç, Hüsnü Çağrı; Çoşkun, Hakkı; Demirtaş, Yıldırımcan; Soylu, Sinan; Kurt, Atilla
Amaç: Kliniğimizde uygulanan Lazer Hemoroidoplasti (LHP) işleminin sonuçlarını sunmayı amaçladık Yöntem: Bu retrospektif çalışmada, kliniğimizde 24 aylık bir dönemde yapılan LHP'nin sonuçlarını analiz ettik. Hastalar, ameliyat sonrası üç haftalık takip ziyaretleri planlandı, ardından üç ve altı aylık aralıklarla takip edildi. Bulgular: 112 hastanın 73'ü (%65.17) erkek, 39'u (%34.82) kadındı. Ortalama yaş 52.6 idi (aralık: 20-65). Ortalama operasyon süresi 18.3 dakika idi (aralık: 12-25). Yetmiş hasta grade 2 olarak sınıflandırılırken, 42 hasta grade 3 olarak sınıflandırıldı. Ortalama hastanede kalış süresi 1.16 gün idi. Grade 2 hemoroidli 70 hastanın 5'i takipten çıkarıldı, 24'ü 6 ay ve 41'i 1 yıl boyunca takip edildi. Bir (1.42%) hasta 1 yıl sonundaki takipte nüks gösterdi. Dört hasta (%9.52) nüks gösterdi. Grade 3 hemoroidli bir hasta, bir hafta süren postoperatif kanama yaşadı ve eritrosit süspansiyonu transfüzyon ihtiyacı oldu. Grade 3 hemoroidli bir başka hasta, konservatif tedavi ile çözülen bir hematom geliştirdi. Grade 3 hemoroidli sekiz hastada (%7.14) postoperatif ödem gelişti, hepsi konservatif tedavi ile iyileşti. Görsel Analog Skala (VAS) kullanılarak ölçülen ağrı değerleri, birinci postoperatif günün 2.03, ikinci postoperatif günün ise 1.49 olarak ölçüldü. Sonuç: LH tedavisi, Grade 2 ve Grade 3 hastalıklar için başarılı bir alternatif tedavi seçeneği olduğu düşünülmektedir ve LH tedavisinde hasta seçiminin kritik önem taşıdığı vurgulanmaktadır.
Neutrophil-to-Lymphocyte Ratio, Platelet-to-Lymphocyte Ratio, and Red Blood Cell Distribution Width as New Biomarkers in Patients with Colorectal Cancer
(AVES, 2017) Cakmak, Erol; Soylu, Sinan; Yonem, Ozlem; Yilmaz, Abdulkerim
Objective: The incidence of colorectal cancer in developed countries has been found to increase with age. Early diagnosis and screening decrease the mortality rates in colorectal cancer. This study aimed to use inflammatory markers neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and red blood cell distribution width (RDW)as new biomarkers for early diagnosis and screening in patients with colorectal cancer. Materials and Methods: A total of 59 patients with colorectal cancer and 59 age-and sex-matched healthy participants were included in the study. Localization, tumor node metastasis (TNM) stage, and preoperative hemoglobin levels, neutrophil counts, lymphocyte counts, platelet counts, and RDW values were obtained from medical records. Using the receiver operating characteristic (ROC) curve, the optimal cutoff levels of the biomarkers were determined. Results: NLR, PLR, and RDW were significantly higher in patients with colorectal cancer than in healthy participants (p< 0.001). According to ROC analysis, the cutoff value for NLR was 2.05 [area under the curve (AUC): 0.740, sensitivity: 78%, specificity: 66%]; the cutoff value for PLR was 130 (AUC: 0.702, sensitivity: 65%, specificity: 72%); and the cutoff value for RDW was 14 (AUC: 0.774, sensitivity: 68%, specificity: 73%). Conclusions: NLR, PLR, and RDW were found to be significantly higher in patients with colorectal cancer than in healthy participants. Therefore, it is recommended that these additional biomarkers can be used for early diagnosis and screening of colorectal cancer.
Our Experience with Emergency Surgery in Geriatric Patients
(Sivas Cumhuriyet Üniversitesi, 2024) Soylu, Sinan
Objective: A significant portion of admissions to emergency departments are made up of elderly patients. We aim to determine the causes of acute abdomen in geriatric patients requiring emergency surgery, evaluate postoperative intensive care needs and mortality rates, and raise awareness about this patient group. Methods: Patient files of individuals aged 65 and older who were consulted in our hospital's emergency department and subsequently admitted to the general surgery service between 2021 and 2023 were retrospectively reviewed. Data including age, gender, underlying diseases, additional illnesses, duration of stay in the intensive care unit, cause of mortality, and length of hospital stay were extracted from the patient's records. The collected data were analyzed statistically for comparisons. Results: Data of 27 patients were accessed.these patients, 40.7% (n=11) were female, and 59.3% (n=16) were male. The average age of the patients was determined to be 74.81 years (ranging from 65 to 88). Thirteen patients, comprising 5 males and 8 females, were monitored in the intensive care unit. 6 patients experienced mortality, and all of them were those admitted to the intensive care unit. When comparing mortality rates based on gender, it was 18.8% in male patients and 27.3% in female patients. All 27 patients had at least one comorbidity. Patients who experienced mortality had at least two comorbidities. Twelve patients did not undergo surgery. Conclisions: In the emergency assessment of geriatric patients, a prompt diagnosis should be made, considering comorbidities after diagnosis, and follow-up and treatments should be adjusted multidisciplinary for patients requiring intensive care or those to be monitored in regular rooms.
Photodynamic activity of cationic ball-type zinc phthalocyanine in cell culture experiments with MCF-7 and MG63 cancer cells
(World Scientific Publ Co Pte Ltd, 2024) Soylu, Sinan; Bagda, Efkan; Altun, Ahmet; Bagda, Esra; Kurt, Atilla; Erden, Fuat; Yabas, Ebru
Effective binding of cationic ball-type zinc phthalocyanine (BT-Pc) to G-quadruplex (G4) DNA molecules has already been demonstrated previously. This motivated us to investigate the PDT performance of BT-Pc in cell culture studies. Accordingly, the present work involves the application of BT-Pc to breast (MCF-7) and osteosarcoma (MG63) cancer cells, and also to healthy fibroblast cells (L929). Cell lines (CL) were irradiated at 640 nm wavelength, and the photodynamic activity was evaluated. The results show that cytotoxicity was significantly stronger in MCF-7 cells than in both L929 and MG63 cells (p < 0.05) in the absence of IPL irradiation. Also, we report that the application of l IPL provided significant photodynamic activity in MCF-7 and MG63 cells (p < 0.05). These results further suggest that BT-Pc might become a promising photosensitizer for future clinical trials.
Role of pentoxifylline and iloprost in the prevention of ischemia-reperfusion injury in an experimental model of intestine ischemia-reperfusion in rats
(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2018) Abakay, Ugur; Soylu, Sinan; Goksel, Sabahattin; Sarac, Bulent; Inan, Zeynep Deniz Sahin; Cakmak, Erol; Korkmaz, Ozge; Kurt, Atilla; Genc, Husnu Cagri
BACKGROUND: Intestinal ischemia-reperfusion (I/R) injury can lead to multiple organ failure and death. The aim of this study was to investigate the effects of pentoxifylline and iloprost administered before reperfusion in intestinal ischemia. METHODS: In total, 25 male Wistar Albino rats weighing 250-300 g were divided into five groups each comprising five subjects: control group (n=5), sham group (n=5, no I/R), I/R group (n=5, 45 min ischemia, and 120 min reperfusion), I/R + pentoxifylline group (n=5, 45 min ischemia following intraperitoneal 50 mg/kg pentoxifylline and 120 min reperfusion), and I/R + iloprast group (n=5, 45 min ischemia followed by intraperitoneal 2 mcg/kg iloprost and 120 min reperfusion). At the end of the experiment, ileum specimens were stained using hematoxylin-eosin and histopathologically evaluated using the Chiu score. Isometric contraction-relaxation responses were recorded using organ baths for contraction-relaxation responses. RESULTS: Pentoxifylline provided a significant improvement in response to histopathological and contraction-relaxation responses. Although iloprost provided recovery in reperfusion injury, it was not statistically significant. CONCLUSION: Our findings demonstrate that pentoxifylline may be promising in preventing small bowel ischemia-reperfusion injury. We concluded that further clinical and experimental studies for iloprost are needed.

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