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Anticancer Activity of Water and Methanol Extracts of Hypericum scabrum L. on Different Cancer Cell Lines
(Horticulture and Forestry Society from Transylvania, 2019) Guclu, Gulsen; Tas, Ayca; Tulimat, Manhal; Eruygur, Nuraniye; Silig, Yavuz
Hypericum scabrum L. is an endemic medicinal plant with antimicrobial, anticancer and anti-inflammatory, antiviral effect. In this study, we aimed to investigate the cytotoxic effect of water and methanol extracts on different cancer cell lines such as osteosarcoma cancer, cervical cancer and prostate cancer. The data showed that the methanol extract had a highly effective cytotoxic effect on all three cell lines. Although water extract caused less cell loss than methanol extract, it was found that the cells were inhibited in all three cell lines. DU-145, which is the prostate cell line, was more effective in both extracts. As a result, the water and methanol extract of Hypericum scabrum L. have an anticancer effect on Saos-2, HeLa and DU-145 cells. There is a need for further and comprehensive studies by isolating the main active ingredient of this plant which is a candidate for drug of cancer in the future. Our study is expected to lead the studies in this direction. © 2019. All Rights Reserved.
Anticancer activity, hTERT expression and telomere length analysis in SH-SY5Y neuroblastoma cell lines applied to docetaxel
(Elsevier B.V., 2023) Inandiklioglu, Nihal; Tas, Ayca; Agbektas, Tugba; Tuncbilek, Zuhal; Raheem, Kayode Yomi; Cinar, Gulcihan; Silig, Yavuz
Neuroblastoma is the most common extracranial solid tumor of infancy in a broad range of clinical courses, ranging from spontaneous regression to fatal progression. Telomere maintenance plays an important role in genome stability and cell proliferation. Telomerase reverse transcriptase (hTERT in humans) is a catalytic subunit of the enzyme telomerase. In this study, it was aimed to determine the anticancer activity of the docetaxel chemotherapeutic agent in neuroblastoma cell line (SH-SY5Y) and to investigate its effect on hTERT gene expression level and telomere length. Molecular docking studies were performed on docetaxel with the crystal structure of telomerase. The electronic properties of docetaxel were calculated using the density functional theory (DFT) method. SH-SY5Y cells were treated for 24, 48 and 72 h with specific concentrations of docetaxel drug ranging from 1 to 100 µg/ml. IC50 doses of docetaxel were determined and administered to SH-SY5Y cells, followed by RNA isolation. hTERT and MYC gene expression levels and telomere length were measured in the docetaxel-treated sample using the RT-PCR method. In addition, theoretical analyzes were made. The IC50 values of docetaxel after 24, 48 and 72 h were 8.32±1.45 μg/ml, 7.67±2.56 μg/ml and 5.51±1.24 μg/ml, respectively. According to the results obtained, docetaxel was found to have the highest activity in 72 h of incubation. It was determined that the docetaxel drug decreased the expression level of the hTERT gene in SH-SY5Y cells. Telomere lengths were significantly reduced in the docetaxel treated SH-SY5Y cell line compared to the control group (p < 0.05). Molecular docking analysis results were in agreement with the experiments. Analysis results indicated a good interaction between docetaxel and the active site of telomerase. The results of this study, reinforced by molecular docking analyzes, might be proved valuable for the development of potent telomerase inhibitors. © 2022
Comparison of Beta-2 Adrenergic Receptor Gene Polymorphisms Between Patients With Fibromyalgia Syndrome and Healthy Controls
(Turkish League Against Rheumatism, 2020) Sen Cakiro, Gozde; Hizmetli, Sami; Silig, Yavuz; Karadag, Ahmet; Hayta, Emrullah; Ozaltin, Burcu; Tas, Ayca
Objectives: This study aims to compare the beta-2 adrenergic receptor (ADRB2) gene polymorphisms of patients with fibromyalgia syndrome (FMS) with those of healthy control subjects, and to investigate the possible relationship between symptoms of FMS and polymorphisms of the ADRB2 gene. Patients and methods: The study included 170 females (mean age 47.8 +/- 10.3 years; range, 21 to 75 years) diagnosed with FMS according to the 2010 American College of Rheumatology criteria and 170 healthy females (mean age 47.2 +/- 8.8 years; range, 20 to 72 years) as the control group. Several clinical symptoms of the participants related to FMS were questioned and recorded. The visual analog scale (VAS) and Fibromyalgia Impact Questionnaire (FIQ) scores of the fibromyalgia group were recorded. In both groups, the ADRB2 (rs1042717) single-nucleotide polymorphism was detected by way of a real-time polymerase chain reaction. The wild-type (Guanine/Guanine), the mutant type (Adenine/Adenine) and heterozygous type (Adenine/Guanine) were detected. The sample power was calculated considering the minor allele frequency. Results: The comparison of the ADRB2 gene polymorphism between patients with FMS and the control subjects showed that the groups were similar in terms of ADBR2 gene polymorphism and genotype (p>0.05). There was no significant difference in terms of genotype when the ADRB2 gene polymorphisms in patients with FMS were compared in terms of clinical symptoms, VAS and FIQ scores (p>0.05). Conclusion: Beta-2 adrenergic receptor (rs1042717) gene polymorphisms and genotype distribution are no different between patients with FMS and healthy individuals. ADRB2 gene polymorphisms in patients with FMS have no effect on clinical symptoms and VAS and FIQ scores. The results of the present study will light the way for future research into ADRB2 gene polymorphisms in the pathogenesis of FMS.
Does the Addition of Poly(glycolide-co-lactide) to Teicoplanin-Containing Poly(methyl methacrylate) Beads Change the Elution Characteristics?
(Springer Heidelberg, 2020) Kilinc, Seyran; Pazarci, Ozhan; Cakmak, Nese Keklikcioglu; Tas, Ayca
Background The objective of our study was to measure and compare the elution characteristics of teicoplanin from poly(methyl methacrylate) PMMA beads with those of poly(glycolide-co-lactide) PGLA-added beads. Methods The study included two groups of PMMA + teicoplanin beads. PMMA was added to teicoplanin in Group 1 and PMMA + PGLA was added to teicoplanin in Group 2. A total of 16 beads of 1 cm(3) were created for each group. Samples were added individually to tubes containing 3 ml of phosphate-buffered saline (PBS). Antibiotic elution was measured by measuring absorbance values of 1-ml samples taken at regular intervals using a UV-Vis spectrophotometer and cumulative percentages of drug release were calculated. In addition, the spectra of teicoplanin were identified using a FTIR spectrophotometer in a wavelength range of 400-4000 cm(-1). Results Drug elution in the PBS medium was measured and compared for Groups 1 and 2. The cumulative percentage of drug release from the PGLA-added beads (Group 2) was significantly higher (p = 0.01). The molecular structure of teicoplanin was also confirmed using FTIR. Conclusion The in vitro results showed that the addition of biodegradable PGLA into bone cement functions as a water-soluble porogen which allows for significant increases in the elution of teicoplanin from cement. This increase in elution suggests that the PGLA would allow for further fluid contact and exchange with the previously entrapped drug. These results may have important clinical applications.
The effects of colostrum on some biochemical parameters in the experimental intoxication of rats with paracetamol
(SPRINGER HEIDELBERG, 2018) Karabacak, Mursel; Kanbur, Murat; Eraslan, Gokhan; Silig, Yavuz; Sarica, Zeynep Soyer; Tekeli, Muhammet Yasin; Tas, Ayca
In the current study, the possible prophylactic and therapeutic effects of colostrum (COL) on acute organ injury caused by paracetamol (PAR) in rats were evaluated. Within the scope of this study, a 2-month-old male (150-200 g) 70 Wistar Albino rat was used and a total of seven groups were designed. The first group (CNT) was maintained for control purposes. The second group (COL-1) was given COL for 1 day, at a dose of 500 mg/kg at 6-h intervals, and blood and tissue sampling was performed at 24 h. The third group (COL-7) received COL for 7 days, at a dose of 500 mg/kg at 6-h intervals on day 1 and at a daily dose of 500 mg/kg on the following days, and blood and tissue samples were taken at the end of seventh day. The fourth group (PAR-1) was administered with PAR at a dose of 1.0 g/kg bw and was blood and tissue sampled at 24 h. The fifth group (PAR-7) received PAR at a dose of 1.0 g/kg bw on day 1 and was blood and tissue was removed at the end of day 7. The sixth group (PAR+COL-1) was administered with a combination of PAR (1 g/kg bw) and COL (500 mg/kg at 6-h intervals), and blood and tissue samples were collected at 24 h. The seventh group (PAR+COL-7) received 1.0 g/kg bw of PAR on day 1 and was given COL throughout the 7-day study period (at a dose of 500 mg/kg at 6-h intervals on day 1 and at a daily dose of 500 mg/kg on the following days). In the seventh group, blood and tissue samples were taken at the end of seventh day. Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), glucose, creatinine, triglyceride, total bilirubin, total protein and albumin levels/activities were analysed in the serum samples. The malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) levels/activities, known as oxidative stress parameters, were assayed for tissue homogenates and blood (erythrocytes/plasma); in addition, enzyme activities of GSH S-transferase (GST), cytochrome P4502E1 (CYP2E1), NADH-cytochrome b5 reductase (CYTB5), glucose-6-phosphate dehydrogenase (G6PD), NADPH-cytochrome P450 C reductase (CYTC) and glutathione (GSH) levels/activities defined as drug metabolising parameters were measured in liver homogenates. In result, it was determined that PAR caused significant alterations in some biochemical and lipid peroxidation parameters and the activities/levels of drug metabolising parameters in the liver and that COL normalised some of these parameters and reduced PAR-induced tissue damage.
Effects of Triclosan-Impregnated Suture Materials on Colonic Anastomosis
(Erciyes Univ Sch Medicine, 2020) Atabey, Mustafa; Tas, Ayca; Topcu, Omer; Silig, Yavuz
Objective: Symptomatic anastomotic leakage observed after colorectal surgery is one of the major complications. One of the factors affecting the anastomosis healing is the type of suture material used. This study aims to investigate the effects of the suture material polydioxanone (PDS, Ethicon), which is late absorbable and has a monofilament structure, and the suture material polyglactin 910 (Vicryl, Ethicon), which is absorbable and has a multifilament structure, on the healing of colonic anastomosis, and to compare the traditional forms of these sutures with their antibacterial effective triclosan-impregnated forms (PDS Plus, Ethicon and Vicryl Plus, Ethicon). Materials and Methods: The rats were divided into four equal groups consisting of 10 subjects each: Group I: Vicryl; Group II: Vicryl Plus; Group III: PDS; and Group IV: PDS Plus. The presence of wound infection, whether the integrity of the abdominal wall was maintained, intra-abdominal adhesion scoring, the presence of intra-abdominal abscess, and whether the macroscopic integrity of anastomosis was maintained were evaluated. Results: According to the results of this experimental study, while the highest ABP and hydroxyproline levels were observed in the PDS Plus group, the lowest values were observed in the Vicryl group. However, the difference was not statistically significant. Conclusion: Slowly absorbable and monofilament PDS suture material causes less tissue reaction and inflammatory response compared to the Vicryl suture material that is absorbable in the colonic anastomosis line and multifilament.
Evaluation of the Relationship Between Mobile Phone Usage and miRNA-574-5p and miRNA-30C-5p Levels in Thyroid Cancer Patients
(Galenos Publ House, 2024) Hasbek, Zekiye; Tas, Ayca; Erturk, Seyit Ahmet; Sariakcali, Baris; Babacan, Ozge Ulas; Duman, Gulhan; Silig, Yavuz
Objectives: This study aimed to evaluate the relationship between mobile phone usage and miRNA-574-5p and miRNA-30C-5p levels in patients diagnosed with differentiated thyroid cancer (DTC). Methods: Fifty patients diagnosed with DTC and 50 healthy volunteers were included in the study. miRNA-574-5p and miRNA-30C-5p gene expression levels in the blood of all subjects were analyzed by real time-polymerase chain reaction, and a questionnaire including various questions was administered to both groups. Results: Although there was a 7.60 -fold increase in miRNA-30C-5p gene expression levels in the patient group compared with the control group, it was not found to be statistically significant. Considering the miRNA-574-5p gene expression levels, although there was a 2.96 -fold increase in the patient group compared with the control group, no significant relationship was found. In our study, 85% of our patients were using mobile phones with internet access, whereas 98% of our healthy volunteers were using mobile phones (p<0.05). While 53.5% of the patients had their mobile phones with them while they were sleeping, this rate was 83.7% in healthy volunteers (p<0.05). However, 93.9% of the healthy volunteers did not have a Wi-Fi device in their bedrooms, and this rate was 75% in the patient group (p<0.05). Conclusion: Although miRNA-30C-5p and miRNA-574-5p gene expression levels were higher in patients than in healthy volunteers, the differences were not statistically significant. Although there was no significant difference in miRNA levels, we believe that due to the higher rate of Wi-Fi device presence in bedrooms in patients compared with healthy volunteers, the effects of electromagnetic radiation on the thyroid can be reduced by paying attention to this simple change.
Expression levels of some genes in the MAPK pathway (DUSP1, DUSP2, DUSP4, DUSP6 and DUSP10) in eyelid tumor tissue
(Walter De Gruyter Gmbh, 2024) Ozmen, Esma; Tas, Ayca; Akin, Dilara Fatma; Erdogan, Haydar; Silig, Yavuz
Objectives To control mitogen-activated protein kinases (MAPK) signaling pathways involved in the onset and progression of cancer, dual specificity phosphatases (DUSPs/MKP) are essential. This study seeks to detect the correlation between eyelid tumors and the genes DUSPs, known for their influence on MAPK signaling pathways. Additionally, we aim to juxtapose our findings with analyses from various bioinformatics databases.Methods Expression levels of relevant genes in cDNA samples were determined by quantitative PCR method. Open-access databases were used for mutation analysis of relevant genes, mRNA expression changes, and survival analyses, and the STRING database was used for protein-protein interactions.Results It was found that the expression of DUSP1 and DUSP2 showed a significant decrease in the tumor tissue, while a significant increase was detected in the DUSP4 and DUSP6 genes. Additionally, when we compared the study genes with the Cancer Genome Atlas program cancer cohorts, it was found that the DUSP1 and DUSP10 gene expression profiles were downregulated in uveal melanoma compared to other cancer cohorts.Conclusions Significant and obvious changes were observed in the DUSP genes we studied in eyelid tumors. However, the relationship between these genes and cancer must be studied more. Considering that these enzymes are effective in cell proliferation, differentiation, and apoptosis, it would be appropriate to plan comprehensive studies on their interactions with other proteins they interact with in the MAPK pathway.
High levels of cathepsin S and cystatin C in patients with fibromyalgia syndrome
(Wiley, 2020) Kocak, Ibrahim; Hizmetli, Sami; Tas, Ayca; Karadag, Ahmet; Zontul, Cemile; Silig, Yavuz
Objectives Although the etiopathogenesis of fibromyalgia syndrome (FM) is not yet clear, central sensitization is thought to be responsible for the pathogenesis of FM. The aim of this study was to compare the serum cathepsin S (CatS) and cystatin C (CysC) levels between patients with FM and healthy control subjects. Methods This study was conducted in the Physical Medicine and Rehabilitation Clinic between January 2019 and October 2019. The study included 145 FM patients newly diagnosed with primary FM according to the 2010 American College of Rheumatology criteria and 129 healthy volunteers. The age, gender, and body mass index (BMI) of the participants were recorded. Venous blood samples were collected from both groups for the measurement of the levels of serum CatS and CysC. The functional status of FM patients was evaluated using the Fibromyalgia Impact Questionnaire (FIQ). Results No statistically significant difference was determined between the patient and control groups in terms of age, gender, and BMI (P > .05). A comparison of the serum CatS and CysC levels of the FM and control groups revealed a statistically significant difference (P = .001). No correlation was determined between FIQ and serum CatS and CysC levels (P > .05). Conclusion Serum CatS and CysC levels were found to be higher in FM patients. However, there was no correlation between the functional status of FM patients and serum CatS and CysC levels. These results can be of guidance for further clinical studies of the etiopathogenesis and treatment of FM.
In vitro cytotoxicity, gene expression, bioinformatics, biochemical analysis, and in silico analysis of synthesized carbonitrile derivatives
(Springer Wien, 2025) Tuzun, Burak; Agbektas, Tugba; Naghiyev, Farid N.; Tas, Ayca; Zontul, Cemile; Ozum, Unal; Khalilov, Ali N.
In this work, an efficient single-step green synthesis protocol of 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles was applied with the purpose of their biological activity studies. It was found that the cytotoxic activities of 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles in the SHSY-5Y cancer cell line were most active after 72 h of incubation. It was determined that 1,6-diamino-4a-methyl-3-oxo-2,8-diphenyl-2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitrile showed higher anticancer activity on SHSY-5Y cancer cells compared to other molecules. The expression levels of Myc-N, Casp2, Tp53, RAD51, BRCA2, MDM2, BAX and NF-kappa B1 genes were analyzed by RT-PCR method by applying 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles to the SHSY-5Y neuroblastoma cancer cell line. It was observed that there were differences in expression levels between study groups and all differences were found to be statistically significant (p < 0.000). Bioinformatics analysis was performed using the STRING v 11 Protein interaction tool. When the molecules in the SHSY-5Y cell line were compared to the control group, an 80% increase in GSH levels was observed in 1,6-diamino-4a-methyl-3-oxo-2,8-diphenyl-2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitrile and 1,6-diamino-8-(4-chlorophenyl)-4a-methyl-3-oxo-2-phenyl-2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitrile groups. SOD enzyme activity increased significantly in the former group compared to the control and other groups. LDH activity was detected at a higher rate in this cell line compared to the control group. Calculations were made for 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles using the Gaussian software package on B3LYP, HF, and M06-2 x levels with the 6-31++g(d,p) basis sets. The activities of the 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles found in the study against SH-SY5Y protein (2F37, 3PBL and 5WIV), comparison has been made. MM/GBSA methods are calculated binding free energy for all molecule and 3PBL protein. Afterwards, ADME/T analysis was performed to examine the effects of the molecules on human metabolism.
An in vitro Study of Cytotoxic Activity of Euphorbia macroclada boiss on Mcf-7 Cells
(ASSOC PHARMACEUTICAL TEACHERS INDIA, 2018) Tas, Ayca; Sahin-Bolukbasi, Serap; Cevik, Esranur; Ozmen, Esma; Gumus, Erkan; Silig, Yavuz
Objective: The study was aimed to evaluate the cytotoxic activity of acetone extract of leaves, flower and body of Euphorbia macroclada boiss on human breast cancer cell line (MCF-7). Material: The cells were plated at a cell density of 1x10(5) cells in 96-well plates and grown with DMEM medium containing supplemented with 10% FBS and 1% penicillin. The cells were treated by different concentrations of acetone extract of Euphorbia macroclada boiss (10-1000 mu g/mL) during 24, 48 and 72 h. The cytotoxic activities of the tested compounds were determined by cell proliferation analysis using standard (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: After the evaluation of cytotoxicity assay results, it is determined that flower and body parts have a significant cytotoxic effect on MCF-7 breast cancer cell line. The values that obtained reading at 570 nm spectrophotometrically, were analyzed with GraphPad Prism7 and IC50 growth inhibition values was determined. Conclusion: The results of MTT assay showed that leaves, flower and body significantly reduced % cell viability comparative to the control. It was also shown that body had more growth inhibitory effect on MCF-7 cell compared to the leaves part.
Investigation of the association between the MDM2 T309G polymorphism and gastric cancer
(SPANDIDOS PUBL LTD, 2017) Tas, Ayca; Atabey, Mustafa; Caglayan, Gulcin; Bostanci, Meric Emre; Bolukbasi, Serap Sahin; Topcu, Omer; Silig, Yavuz
Murine double minute clone 2 oncoprotein (MDM2) is a key component in the regulation of the tumour suppressor p53. The association between the MDM2 polymorphism and gastric cancer (GC) has been investigated in Turkish population. In the present case-control study, the aim was to investigate the association between genetic polymorphisms of the MDM2 gene (a major regulator of p53 function) and primary GC risk in a Turkish population. The polymorphism, T309G (rs2279744) in the MDM2 gene was determined in patients with GC (n=65) and in healthy control subjects (n=67) using the polymerase chain reaction-restriction fragment length polymorphism method. The findings were evaluated using logistic regression and.2 tests. No statistically significant differences were observed between the control subjects and patients with GC regarding smoking status. A comparison between GC cases and control subjects indicated a statistically significant difference for family history of cancer [odds ratio (OR)=0.17; 95% confidence interval (CI), 0.05-0.56;.2=0.19; P=0.01]. A significant difference was identified in the GG genotype distribution between GC patients and control subjects (OR=4.58; 95% CI, 1.18-17.79; P=0.022). Thus, the results of the present study indicate that the MDM2 gene T309G intron (GG) genotype may be an important risk factor for GC development in the Turkish population.
Is there a relation between Murine double minute 2 T309G polymorphism and lung cancer risk in the Turkish population?
(WALTER DE GRUYTER GMBH, 2017) Yilmaz, Meral; Tas, Ayca; Kacan, Turgut; Sari, Musa; Silig, Yavuz
Introduction: Association between Murine double minute 2 T309G polymorphism and lung cancer risk has been investigated in several populations, but results of these studies are inconsistent. We aimed to investigate the effect of Murine double minute 2 T309G polymorphism on development of lung cancer in a Turkish population. Methods: Total 200 subjects including 100 patients and 100 controls were analyzed and used polymerase chain reaction and restriction fragment length polymorphism methods for genotyping analysis of the polymorphism. Results: We found that smokers compared with nonsmokers have approximately eight fold higher lung cancer risk [p = 0.0001, OR = 8.27 (4.02-16.9)]. Frequency of GG genotype was higher in patients than in controls, but this ratio was not significant (chi(2) = 3.5, p = 0.17). Genotype distribution of the polymorphism was not different neither patients with non-small cell lung cancer nor patients with small cell lung cancer (chi(2) = 2.89, p = 0.57). We analyzed together with demographic feature (except smoking habit), clinicopathological findings and genotype frequencies of this polymorphism, and any association with lung cancer risk was not obtained. Conclusion: No correlation between Murine double minute 2 T309G polymorphism and lung cancer risk was detected in this Turkish population.
Leptin/Melanocortin Pathway in Cholelithiasis Patients: A Diagnostic Perspective
(Bentham Science Publ Ltd, 2025) Agbektas, Tugba; Guclu, Gulsen; Tas, Ayca; Ozmen, Esma; Topcu, Omer; Aydin, Suleyman; Silig, Yavuz
Background: Cholelithiasis is the most prevalent inflammatory condition of the gallbladder. The regulation of biological processes, including energy homeostasis, and control of body weight are key mechanisms that the leptin and melanocortin pathways play a role in Cholelithiasis is the most prevalent inflammatory condition of the gallbladder. There are various risk factors for the development of gallstone disease, especially weight gain, and obesity is just one of them. This risk factor can be minimized by maintaining appetite and energy balance. Here, leptin and melanocortin pathways are the key mechanisms in maintaining appetite and energy homeostasis. Objectives: The aim of this study was to investigate the relationship between the levels of LEP, LEPR, TrkB, BDNF, POMC, and MC4R proteins in patients with Cholelithiasis. This study aims to determine the relationship between LEP, LEPR, TrkB, BDNF, POMC, and MC4R protein levels, which play a role in maintaining appetite and energy homeostasis, and cholelithiasis. Methods This study examined 44 patients diagnosed with Cholelithiasis and 44 healthy control subjects who had not previously been diagnosed with any form of Cholelithiasis. The levels of leptin (LEP), Leptin Binds To Leptin Receptors (LEPR), Tropomyosin Receptor Kinase B (TrkB), Brain-Derived Neurotrophic Factor (BDNF), Pro-OpioMelanoCortin (POMC), and Melanocortin-4 Receptors (MC4R) molecules were analyzed using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The results were analyzed using the SPSS Software (Version 22.0) program and GraphPad Prism 8.0.1 software. Results: The study found a statistically significant decrease (p < 0.05) in MC4R, TrkB, BDNF, and POMC protein levels in Cholelithiasis patients compared to the control group. There was no statistically significant difference in LEP and LEPR concentration values between the two groups (p = 0.247, p = 0.674). Conclusion: The proteins MC4R, TrkB, BDNF, and POMC, which are involved in the leptin and melanocortin pathways may play a significant role in Cholelithiasis disease. However, more detailed research on the relevant proteins is needed. Nevertheless, this research will guide new studies.
Manganese-superoxide dismutase (MnSOD) polymorphisms
(WALTER DE GRUYTER GMBH, 2015) Silig, Yavuz; Tas, Ayca; Pinarbasi, Hatice
Objective: Manganese superoxide dismutase (MnSOD, SOD2), the only known superoxide scavenger in mitochondria, may be particularly important for antioxidant defense because mitochondria are the major sites for cellular metabolism and hence production of reactive oxygen species. Methods: In this study, 440 Turkish individuals were genotyped for polymorphisms of SOD2 gene. The distribution of these polymorphisms in this population was examined using a PCRRFLP method. Results: In the present study, a total of 440(females: 201, 46% and males: 239, 54%) healthy individuals were studied. The mean age of the study population was 54,41 +/- 5,76 years (males, 55,34 +/- 5,76; females, 53,12 +/- 7,16). The observed genotype frequencies of SOD2 were 17.5, 50.5 and 32.0% for CC, CT and TT, respectively. Conclusion: This study provides basic information about the allele and genotype frequency distributions of polymorphisms of rs4880 in the SOD2 gene studied. These frequencies may be useful parameters as a reference for future studies on genetic basis of various diseases and cancer susceptibility.
PEGylated Titanium Dioxide Nanoparticle-bound Doxorubicin and Paclitaxel Drugs Affect Prostate Cancer Cells and Alter the Expression of DUSP Family Genes
(Bentham Science Publ Ltd, 2025) Tuncbilek, Zuhal; Cakmak, Nese Keklikcioglu; Tas, Ayca; Ayan, Durmus; Silig, Yavuz
Background PC is among the cancer types with high incidence and mortality. New and effective strategies are being sought for the treatment of deadly cancers, such as PC. In this context, the use of nanocarrier systems containing titanium dioxide can improve treatment outcomes and increase the effectiveness of anticancer drugs.Objective This study aimed to evaluate the cytotoxic activity of doxorubicin (DOX) and paclitaxel (PTX) drugs on the prostate cancer (PC) cell line by attaching them to pegylated titanium dioxide nanoparticles and to examine their effect on the expression levels of dual-specificity phosphatase (DUSP) genes.Methods Free DOX and PTX drugs, DOX and PTX compounds bound to the pegylated titanium dioxide system were applied to DU-145 cells, a PC cell line, under in vitro conditions, and MTT analysis was performed. Additionally, the IC50 values of these compounds were analyzed. In addition, the expression levels of DUSP1, DUSP2, DUSP4, DUSP6, and DUSP10 genes were measured using RT-PCR. Additionally, bioinformatics and molecular docking analyses were performed on DUSP proteins.Results The cytotoxic activity of PTX compound bound to PEGylated TiO2 was found to be higher than that of DOX compound bound to PEGylated TiO2. Additionally, when the expression levels were compared to the control group, the expression levels of DUSPs were found to be lower in the drugs of the drug carrier systems.Conclusion Accordingly, it was predicted that the pegylated titanium dioxide nano-based carrier could be effective in PC.
Role of SOD2 Ala16Val polymorphism in primary brain tumors
(SPANDIDOS PUBL LTD, 2019) Tas, Ayca; Silig, Yavuz; Pinarbasi, Hatice; Gurelik, Mustafa
The present study aimed to investigate the possible association between the genetic polymorphism of the enzyme superoxide dismutase 2 (SOD2, also known as manganese-dependent SOD), Ala16Val (rs4880), and primary brain tumor risk in the Turkish population. Frequency of the SOD2 gene rs4880 polymorphism was identified in 225 Turkish individuals (120 controls and 105 patients with primary brain tumor) by polymerase chain reaction-restriction fragment length polymorphism. Subject demographics and clinical characteristics were also recorded. The findings were evaluated using logistic regression and chi(2) tests. Logistic regression analysis indicated that smoking did not increase the risk for primary brain tumor [ odds ratio (OR)= 0.77, 95% confidence interval (CI)= 0.44-1.33, chi(2)= 0.352, P= 0.860]. Similarly, there was no statistically significant difference in the family history of cancer incidence between the control subjects and the primary brain tumor patients (OR= 0.81, 95% CI= 0.39-1.71, chi(2)= 0.340, P= 0.560). There was no significant association of the histopathological type, genotype/ allele frequencies and inheritance models of tumor with the gene variants among the patients with primary brain tumor. In summary, the results of the present study indicated that the Ala16Val polymorphism of the SOD2 gene was not associated with primary brain tumor risk in the Turkish population studied.
Serum Calcitonin Gene-Related Peptide and Receptor Protein Levels in Patients With Fibromyalgia Syndrome: A Cross-Sectional Study
(Turkish League Against Rheumatism, 2020) Korucu, Ragip Ulvi; Karadag, Ahmet; Tas, Ayca; Ozmen, Esma; Hayta, Emrullah; Silig, Yavuz
Objectives: This study aims to compare the serum calcitonin gene-related peptide (CGRP) and CGRP receptor protein levels between patients with fibromyalgia syndrome (FM) and healthy control subjects. Patients and methods: The study included 88 patients (7 males, 81 females; mean age 44.5 +/- 9.1 years; range, 20 to 72 years) newly-diagnosed with FM according to the 2010 American College of Rheumatology criteria and 88 healthy volunteers (6 males, 82 females; mean age 43.0 +/- 6.1 years; range, 20 to 57 years). Venous blood samples were collected from both groups for the measurement of the levels of serum CGRP and CGRP receptor proteins (receptor component protein [RCP], receptor activity modifying protein 1 [RAMP 1] and calcitonin receptor-like receptor [CLR]). Results: A comparison of the serum CGRP, CLR and RCP levels of the FM and control groups revealed a statistically significant difference (p=0.001, p=0.005, p=0.001, respectively). The difference between the groups in respect of the serum RAMP 1 levels was not statistically significant (p=0.107). Conclusion: The serum CGRP, CLR and RCP levels were found to be higher in the FM patients, but no difference was determined between the FM patients and the healthy control group in respect of the RAMP 1 level. These results can be of guidance for further clinical studies of the etiopathogenesis and treatment of FM.
Significant association between MDM2 T309G polymorphism and colorectal cancer
(IMPRIMATUR PUBLICATIONS, 2019) Atabey, Mustafa; Tas, Ayca; Sari, Ismail; Bostanci, Meric Emre; Topcu, Omer; Silig, Yavuz
Purpose: The Murine double minute 2 (MDM2) gene plays a crucial role in regulating and suppressing the function of apoptotic pathway. We investigated the relationship between MDM2 gene SNP309 (T309G) (rs2279744) polymorphism and colorectal cancer (CRC) in a Turkish population. Methods: The polymorphism T309G (rs2279744) in the MDM2 gene was studied in patients with colorectal cancer (n=135) and healthy control subjects (n=145) using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method The findings were evaluated using logistic regression and x(2) tests. Results: When CRC cases and controls were evaluated based on different habits and family cancer histories, a statistically significant relationship was found between CRC and alcohol consumption (x(2) =4.07, p=0.044). Cancer cases and controls had statistically significant different family histories of cancer (x(2) =6.82, p =0.009). There was also significant difference in TG genotype distribution in the MDM2 T309G polymorphism between those with and without cancer (OR=1.98, 95% CI =1.98-3.91, x(2) =4.00, p =0.045). Conclusions: The SNP309 polymorphism of the MDM2 gene is associated with increased CRC risk in the Turkish population.
Superoxide Dismutase 1 (SOD 1) A251G Polymorphism
(WALTER DE GRUYTER GMBH, 2017) Silig, Yavuz; Tas, Ayca; Sahin-Bolukbasi, Serap; Caglayan, Gulcin; Sari, Ismail
Objective: A genetic polymorphism of SOD1 A251G(rs2070424) is in the 3rd intron region of the SOD gene. The aim of this study was to determine the frequencies of the polymorphisms of the SOD1 A251G in a Turkish population, including 494 healthy individuals. Methods: The 494 Turkish individuals were genotyped for polymorphisms of SOD1 gene. The distribution of SOD1 A251G polymorphisms in this population was examined using a PCR-RFLP method. Genotype and allele frequencies were estimated by counting. Hardy-Weinberg equation between expected and observed genotype distributions was assessed using the X-2 test. Results: In the present study, the distribution of SOD1 A251G polymorphisms in a Turkish population including 494 (females: 278, 56.3% and males: 216, 43.7%) healthy individuals was examined. The mean age of the study population was 38.4 +/- 16.6 years (males, 39.8 +/- 17.1; females, 37.3 +/- 16.1). The observed genotype frequencies of SOD1 A251G were 86.2, 13.4 and 0.4% for AA, AG and GG, respectively. Conclusions: This study provides basic information about the allele and genotype frequency distributions of polymorphisms in the SOD1 A251G gene studied. These frequencies may be useful parameters as a reference for future studies on genetic basis of various diseases and cancer susceptibility.

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