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Alendronate enhances osseous healing in a rat calvarial defect model
(PERGAMON-ELSEVIER SCIENCE LTD, 2012) Toker, Hulya; Ozdemir, Hakan; Ozer, Hatice; Eren, Kaya
Aim: The aim of this study was to evaluate the effect of alendronate on osseous wound healing in an experimental model. Methods: Critical size defects were created in calvaria of 40 male Wistar rats. The animals were divided into four groups of 10 animals each: autogenous bone graft group; autogenous bone graft with systemic alendronate group (0.01 mg /kg body weight per day for 8 weeks); autogenous bone graft with local alendronate group (1 mg/mL for 5 min); non-treatment (control) group. Animals were sacrificed after 8 weeks; osteoblast number, lamellar bone formation, and area of newly formed bone were analysed. Results: The osteoblast number significantly increased in the autogenous bone graft with local alendronate group compared to the autogenous bone graft group (p < 0.05). Both systemic and local application of the alendronate significantly increased the new bone formation compared to the autogenous bone graft group (p < 0.05) with no significant difference between local or systemic use (p > 0.05). Local alendronate and autogenous bone graft use significantly increased the total bone area compared to autogenous bone graft alone (p < 0.05). Conclusion: Alendronate enhances the new bone formation by autogenous bone graft in the rat calvarial defect model suggesting that the inhibition of the osteoclastic activity allows an increased rate of bone apposition, which could be applicable to the inflammation-induced destruction of the periodontal tissues during disease. (C) 2012 Elsevier Ltd. All rights reserved.
ANALYSIS OF IL-6, IL-10 AND NF-kappa B GENE POLYMORPHISMS IN AGGRESSIVE AND CHRONIC PERIODONTITIS
(NATL INST PUBLIC HEALTH, 2017) Toker, Hulya; Gorgun, Emine Pirim; Korkmaz, Ertan Mahir
Objective: Pro-inflammatory cytokines, interleukin-6 (IL-6), demonstrated to be suppressed by interleukin-10 (IL-10) are known to be regulated by the transcription factor nuclear factor-kappa B(NF-kappa B). The aim of this study was to ascertain the association between genetic polymorphism of these genes (IL-6(-174), IL-10(-597) and NF-kappa B1-94ins/del)) and chronic/aggressive periodontitis. Methods: Forty-five patients with chronic periodontitis (CP), 58 patients with aggressive periodontitis (AP) and 38 periodontally healthy subjects were included in this study. Genomic DNA was isolated from whole blood samples. The NF-kappa B, IL-6, and IL-10 polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: Among subjects for the ins/ins genotypes of NF-kappa B1 gene, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis group than in healthy controls (p = 0.023). A statistically significant difference in genotyping frequencies between AP group and healthy controls was observed for the IL-6 gene. The AA genotype of IL-10 was overrepresented in CP and AP groups compared to healthy controls (OR = 9.93, 95% CI: 2.11-46.7, OR = 5,7, 95% Cl: 1.22-26.89, respectively). Conclusions.. Within the limits of this study, it can be concluded that the IL-10 (-597) AA genotype is associated with susceptibility to chronic/ aggressive periodontitis and IL-6 (-174) GG genotypes and G allele seems to be associated with aggressive periodontitis. Clinical relevance: The results of the current study indicate that IL-6 and IL-10 genotypes seem to be associated with aggressive periodontitis. Also, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis subjects with carrying NF-kappa B1 ins/ins genotypes.
Biocompatibility of fiber-reinforced composite (FRC) and woven-coated FRC: an in vivo study
(Springer Heidelberg, 2023) Nalbantoglu, Ahmet Mert; Eren, Kaya; Yanik, Deniz; Toker, Hulya; Tuncer, Ersin
Objectives To investigate biocompatibility and bone contact area of FRC and woven-coated FRC (FRC-C) in rats. Materials and methods Sixty rats were allocated to three groups: FRC (n=20), FRC-C (n=20), and control group (n=20). Subgroups were determined as 4th (n=10) and 12th weeks (n=10). The specimens were placed in the femur of rats. In the control group, the bone defects were left empty and sutured. Four and 12 weeks after implantation, the rats were sacrificed. Histopathological examinations were performed in a semi-quantitative manner. Twenty rats (n=20) were used for scanning electron microscopy (SEM) examination. Bone contact surfaces were calculated in SEM analysis. A chi-square test was performed to analyze the data. Results No statistical difference was detected between the 4th and 12th weeks in the quality of bone union. Quality of bone union was lower in FRC compared to the control group in the 4th week (p=0.012) and the 12th week (p=0.017). The periosteal reaction at the 12th week was lower in FRC than in the control group (p=0.021). Bone contact of FRC and FRC-C was 85.5% and 86.3%, respectively. Conclusions FRC and FRC-C were biocompatible and showed no inflammation. The woven coating did not increase the quality of bone union and bone contact area, while not reducing biocompatibility.
A comparative evaluation of the systemic and local alendronate treatment in synthetic bone graft: a histologic and histomorphometric study in a rat calvarial defect model
(ELSEVIER SCIENCE INC, 2012) Toker, Hulya; Ozdemir, Hakan; Ozer, Hatice; Eren, Kaya
Objective. The purpose of this study was to compare the relative efficacy of systemic and local alendronate treatment of synthetic bone graft in a rat calvarial defect model. Study Design. Forty Wistar rats were divided into 4 groups: experimental animals received alendronate systemically or locally combined with micro-macroporous biphasic calcium phosphate (MBCP) graft material. In the control group, the defect was left empty. On each animal, a 5-mm standardized bone defect was created with a standard trephine bur in calvarium. All animals were killed after 8 weeks. The number of osteoclasts, osteoclast morphology, resorption lacunae, osteoblastic activity, and lamellar bone formation were histopathologically evaluated and the newly formed bone area was analyzed histomorphometrically. Results. Eight weeks after surgery, the number of osteoclasts and the resorption lacunae in the MBCP group using systemic alendronate therapy was significantly higher than those of the other groups (P < .05). Osteoblast number in the MBCP group using systemic alendronate treatment was significantly increased (P < .05). No significant difference was found among all MBCP groups using local or systemic alendronate treatments with regard to new bone formation (P > .05). Conclusions. Within the limits of the study, alendronate, when administered systemically or locally, did not increase bone regeneration with MBCP graft in the rat calvarial defect model. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114(suppl 5):S146-S152)
Dramatic oral findings belonging to a pica patient: A case report
(WILEY, 2009) Toker, Hulya; Ozdemir, Hakan; Ozan, Fatih; Turgut, Mehmet; Goze, Fahrettin; Sencan, Mehmet; Kantarci, Alpdogan
Pica is a compulsive eating of non-nutritive substances and can have serious medical implications. The causes are not known, but the symptom is often associated iron-deficiency. Pica is an under-diagnosed problem. Although few dental problems associated with pica have been reported, because of its potential impact on blood measures, dental professionals must be aware of this common, but commonly missed, condition. This is an unusual case of a patient diagnosed with aggressive periodontitis (AgP) who had a dirt eating habit (geophagia) and was diagnosed as having pica.
The effect of boron on alveolar bone loss in osteoporotic rats
(ELSEVIER TAIWAN, 2016) Toker, Hulya; Ozdemir, Hakan; Yuce, Hatice Bald; Goze, Fahrettin
Background/purpose: The aim of this study is to investigate the effects of systemically administered boric acid on osteoporosis-related bone alterations, alveolar bone loss, receptor activator of nuclear factor kappa-b ligand (RANKL) expressions, and mandibular bone density in experimental periodontitis model in osteoporotic rats. Materials and methods: Thirty-six male Wistar rats were separated into five study groups: non-ligated control (C, n = 6) group; periodontitis (P, n = 6) group; osteoporosis (0, n = 8) group; osteoporosis + periodontitis n = 8) group, and osteoporosis + periodontitis with 50 mg/kg/d boric acid (BA50, n = 8) group for 15 days. Osteoporosis was created with intraperitoneal injection of 80 mg/kg retinoic acid for 15 days. Silk ligatures (4/0) were placed around the mandibular right first molar teeth to induce experimental periodontitis. After induction of osteoporosis and periodontitis, rats were sacrificed at Day 15. Alveolar bone loss was evaluated with a stereomicroscope by measuring the distance from the cement-enamel junction to the alveolar crest. Density measurements were performed on radiographs. RANKL and tartrate resistant acid phosphatase (TRAP) staining were performed on histological slides. Results: Alveolar bone loss was significantly higher in the O+P group than those of the other groups (P < 0.05). Boric acid decreased bone loss (P < 0.05). TRAP + osteoclast numbers were highest in the P group and lowest in the control group. The differences in TRAP + osteoclast numbers among control, P, O+P, and BA50 groups were significant (P < 0.05). There were no significant differences in RANKL expression and mandibular bone density (P > 0.05). Conclusion: Within limitations of this study, we conclude that boric acid may decrease alveolar bone loss in a rat model with periodontitis and osteoporosis. Copyright (C) 2016, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license.
Effect of Chronic Periodontitis on Serum and Gingival Crevicular Fluid Oxidant and Antioxidant Status in Patients With Familial Mediterranean Fever Before and After Periodontal Treatment
(AMER ACAD PERIODONTOLOGY, 2014) Bostanci, Vildan; Toker, Hulya; Senel, Soner; Ozdemir, Hakan; Aydin, Huseyin
Background: The aim of this study is to investigate the impact of periodontal status on oxidant/antioxidant status in patients with chronic periodontitis (CP) who experienced familial Mediterranean fever (FMF) and their response to non-surgical periodontal therapy. Methods: Data were obtained from 13 patients with FMF with generalized CP (FMF-CP), 15 systemically healthy patients with generalized CP, 15 systemically and periodontal healthy controls (HCs), and 14 periodontally healthy patients with FMF (FMF-HC). Each participant's total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in their gingival crevicular fluid (GCF) and serum were recorded. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks after periodontal treatment were recorded. Results: The study showed statistically significant improvement of clinical parameters in both FMF-CP and CP groups after periodontal treatment. The baseline GCF-TOS and OSI levels were significantly higher in the CP group compared with the FMF-CP group (P < 0.05). After periodontal treatment, the GCF-TOS levels were significantly reduced in members of the FMF-CP group (P < 0.05). The GCF-TAS levels in members of the FMF-CP group were significantly higher than those of members of the HC group at baseline (P < 0.05). Serum-TAS levels in the FMF-CP group were significantly higher than those in the CP and HC groups at baseline (P < 0.05). The GCF-TOS level in the FMF-CP group was significantly higher than that in the FMF-HC group at baseline and 6 weeks. However, there were no significant differences in the serum-TOS and serum-OSI levels of those in the FMF-CP and CP groups at baseline and 6 weeks (P > 0.05). Conclusion: The results of the present study show that patients with FMF-CP displayed reduced oxidative stress and increased antioxidant status compared with those in the CP and HC groups.
The effect of colchicine on alveolar bone loss in ligature-induced periodontitis
(SOCIEDADE BRASILEIRA DE PESQUISA ODONTOLOGICA, 2019) Toker, Hulya; Yuce, Hatice Balci; Yildirim, Ali; Tekin, Mehmet Bugrul; Gevrek, Fikret
Colchicine is widely used in the treatment of several inflammatory diseases due to its anti-inflammatory effect, but effects on bone metabolism are unclear. The aim of this study was to evaluate the effects of systemically-administered colchicine on healthy periodontium and experimentally-induced periodontitis. In total, 42 male Wistar rats were included in this study. A non-ligated group constituting the negative control group (Control, C, n = 6) and a ligature-only group forming the positive control group (LO, n = 12) were created separately. Twelve rats were treated with 0.4 mg/kg colchicine and another 12 with 1 mg/kg colchicine. In the colchicine-administered groups, right mandibles constituted the ligated groups (1 mgC-L or 0.4 mgC-L) and left mandibles formed the corresponding non-ligated controls (1mgC or 0.4mgC). Silk ligatures were placed at the gingival margin of the lower first molars. The animals were euthanized at different time-points of healing (11 or 30 days). Alveolar bone loss was clinically measured and TRAP+ osteoclasts, osteoblastic activity, and MMP-1 expression were examined histologically. There was no increase in alveolar bone loss with either colchicine dose in healthy periodontium (p > 0.05) and the highest level of alveolar bone loss, TRAP+ osteoclast number, and MMP-1 expression were measured in the LO group (p < 0.05). The 0.4 mgC-L group showed less alveolar bone loss at 11 days (p < 0.05), but greater loss at 30 days. The 1 mgC-L group showed higher osteoblast number than the other ligated groups (p < 0.05) at both time-points. In summary, colchicine did not increase alveolar bone loss in healthy periodontium and also may tend to reduce periodontitis progression. However, further extensive study is necessary to understand the mechanism of colchicine action on alveolar bone loss in periodontitis.
Effect of meloxicam on gingival crevicular fluid IL-1beta and IL1 receptor antagonist levels in subjects with chronic periodontitis, and its effects on clinical parameters
(SPRINGER HEIDELBERG, 2006) Toker, Hulya; Marakoglu, Ismail; Poyraz, Omer
The aim of the present study was to determine the effects of meloxicam after initial periodontal treatment on interleukin-1beta (IL-1 beta) and IL-1 receptor antagonist (IL-1ra) in gingival crevicular fluid (GCF) and clinical parameters in the chronic periodontitis patients. Data were obtained from 30 patients with chronic periodontitis. Fifteen chronic periodontitis patients received 7.5 mg meloxicam, and 15 patients received placebo tablets in a 1x1 regimen for 1 month. All subjects were nonsmokers and had not received any periodontal therapy. The plaque index (PI), gingival index (GI), probing depth (PD), and clinical attachment level (CAL) were recorded. The GCF was collected using a paper strip: eluted and enzyme-linked immunoabsorbent assays (ELISAs) were performed to determine the cytokine levels. The clinical data and GCF samples were obtained after periodontal therapy and 1 month after periodontal therapy. The PI, GI, PD, and GCF IL-1ra decreased significantly (p < 0.05) in meloxicam group at first month when comparing the initial levels. While decrease of the PI was statistically significant in control group (p < 0.05), statistically significant changes were not determined in the other clinical parameters and GCF cytokine levels (p > 0.05). There were no significant differences between two groups in any of the investigated parameters. Our observations did not reveal any influence of meloxicam on levels of IL-1 beta and IL-1ra in chronic periodontitis. Additional clinical studies are advisable to determine whether COX-2 selective drugs alter periodontal disease outcome with greater safety.
Effect of Non surgical Periodontal Treatment on Gingival Crevicular Fluid Interleukin-1beta and Interleukin-37 in Different Periodontal Diseases
(Galenos Yayincilik, 2021) Gorgun, Emine Pirim; Toker, Hulya; Poyraz, Omer
Objective: Interleukin-37 (IL-37), which is a natural inhibitor of innate immunity, is correlated with chronic inflammation and some autoimmune diseases. Proinflammatory cytokines, for example, interleukin-1beta (IL-1 beta), which plays a key part in the periodontium, can induce IL-37. This study aimed to investigate the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) IL-37 and IL-1 beta levels in generalized aggressive periodontitis (G-AgP) and generalized chronic periodontitis (GCP). Materials and Methods: Thirty-five patients with G-AgP, 30 patients with GCP and 30 volunteers with healthy periodontal status (C) were included in this research. Patient groups received scaling and root planning with periodontal hand instrumentations. Clinical periodontal parameters and GCF samples were collected at baseline and at six weeks following SRP. Biomarker levels in GCF were analysed by enzyme-linked immunosorbent assay. Results: Both periodontitis groups exhibited significant improvement in clinical parameters (p<0.05). GCF IL-37 levels were significantly higher in G-AgP and GCP groups than in the C group at baseline (p<0.05). Following periodontal treatment, GCF IL-37 levels were decreased and comparable in all groups. The GCF IL-1 beta levels were significantly higher at baseline in the G-AgP group than those in GCP and C groups, and after periodontal therapy, a decrease was detected in GCF IL-1 beta levels. GCF levels of IL-1 beta were positively correlated with GCF IL-37 levels. Conclusion: Clinical improvement was found in both periodontitis groups after periodontal treatment. IL-1 beta is a key regulator of periodontal inflammation and bone resorption. According to the results of our research, IL-37 may be useful marker of determining the clinical health and disease status of patients with periodontitis. However, further studies are required to determine the mechanism of IL-37 in periodontal inflammation.
Effect of periodontal treatment on IL-1 beta, IL-1ra, and IL-10 levels in gingival crevicular fluid in patients with aggressive periodontitis
(WILEY, 2008) Toker, Hulya; Poyraz, Omer; Eren, Kaya
Aim:The aim of this study was to examine the effect of phase I periodontal treatment on the levels of interleukin (IL)-1 beta, IL-1ra, and IL-10 in gingival crevicular fluid (GCF) in patients with generalized aggressive periodontitis (G-AgP). Material and Methods: Data were obtained from 15 patients with aggressive periodontitis and 15 healthy controls. GCF was collected from at least four pre-selected sites (one shallow, at least two moderate, or at least one deep pockets) in patients with G-AgP. In the healthy group, GCF samples were collected from one site. The cytokine levels were determined by an enzyme-linked immunosorbent assay. Probing depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. The GCF sampling and clinical measurements were recorded at baseline and 6 weeks later after periodontal treatment. Results: IL-1 beta levels were significantly higher at the moderate and deep pocket sites compared with the shallow sites (p < 0.05). After periodontal therapy, IL-1 beta levels were significantly reduced in the moderate and deep pocket sites (p<0.05). IL-1ra levels at baseline of the moderate and deep pocket sites were significantly lower than the control sites (p < 0.05). IL-10 levels were similar in all pockets and did not change after periodontal therapy. Conclusions: The periodontal treatment improves the clinical parameters in G-AgP, and this improvement is evident in deep pocket sites for pocket depth and CAL values. These results confirm that IL-1 beta is effective for evaluating the periodontal inflammation and can thus be used as a laboratory tool for assessing the activity of periodontal disease.
The effects of IL-10 gene polymorphism on serum, and gingival crevicular fluid levels of IL-6 and IL-10 in chronic periodontitis
(UNIV SAO PAULO FAC ODONTOLOGIA BAURU, 2018) Toker, Hulya; Gorgun, Emine Pirim; Korkmaz, Ertan Mahir; Yuce, Hatice Balci; Poyraz, Omer
Objective: Anti-inflammatory cytokines play a crucial role in periodontitis by inhibiting synthesis of pro-inflammatory cytokines. The purpose of this study was to evaluate the effect of interleukin-10 (-597) gene polymorphism and genotype distributions on chronic periodontitis (CP) development and IL-6 and IL-10 levels in gingival crevicular fluid (GCF) and serum before and after non-surgical periodontal treatment. Material and Methods: The study population consisted of 55 severe generalized CP patients as CP group and 50 healthy individuals as control group. Plaque index, gingival index, probing depth and clinical attachment level were recorded and GCF and blood samples were taken at both the baseline and the sixth week after non-surgical periodontal treatment. PCR-RFLP procedure was used for gene analyses and cytokine levels were measured via ELISA. Results: IL-10 genotype distribution was significantly different between CP and control groups (p=0.000, OR: 7, 95% CI, 2.83-60.25). Clinical measurements significantly improved in the CP group after periodontal treatment (p<0.05). Periodontal treatment significantly decreased GCF IL-6 and IL-10 levels. No significant difference was found in clinical parameters between IL-10 AA and AC+CC genotypes at both the baseline and the sixth week (p>0.05). Sixth week GCF IL-10 levels were significantly lower in patients carrying IL-10 AC+CC genotype compared to the patients carrying IL-10 AA genotype (p<0.05). Serum IL-6 and IL-10 levels were lower in patients carrying the IL-10 AA genotype compared to patients with IL-10 AC+CC genotype, but the difference was not significant (p>0.05). Conclusion: IL-10 AA genotype carriers had lower IL-6 and IL-6/10 levels in serum; however, GCF IL-6/10 levels were similar in both genotypes. Within the limitations of our study, a possible association between IL-10(597) gene polymorphism and CP might be considered.
The effects of non-surgical periodontal therapy on oxidant and anti-oxidant status in smokers with chronic periodontitis
(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Akpinar, Aysun; Toker, Hulya; Ozdemir, Hakan; Bostanci, Vildan; Aydin, Huseyin
Aim: The aim of this study was to determine the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) and serum oxidant-antioxidant levels in smoking and non-smoking patients with chronic periodontitis. Methods: Twenty-nine patients with chronic periodontitis (15 smokers (CP-S) and 14 nonsmokers (CP-NS)) and 20 periodontally healthy subjects (10 smokers (H-S) and 10 non-smokers (H-NS)) totalling 49 subjects were included in this study. GCF was collected from at least two pre-selected sites (one moderate and one deep pocket) in patients with CP. In the healthy group, GCF samples were collected from one site. Probing pocket depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. To determine serum total oxidant status (TOS) and total antioxidant status (TAS), venous blood was drawn from each subject. The GCF, serum sampling, and clinical measurements were recorded at baseline and 6 weeks after periodontal treatment. Results: The study showed statistically significant improvement of clinical parameters after periodontal treatment in both smokers and non-smokers. In the CP-S group, there were no significant differences in GCF TAS levels at both moderate and deep pocket sites between baseline and 6 weeks (p > 0.05). GCF TAS levels in the CP-NS groups were significantly increased (p < 0.05) at moderate and deep pocket sites between baseline and 6 weeks. GCF TOS levels in the CP-S groups were significantly decreased (p < 0.05) at deep pocket sites between baseline and 6 weeks. There was no significant difference in serum TAS levels of the all periodontitis patient groups between at baseline and 6 weeks (p > 0.05). Serum TOS levels in the CP-S and CP-NS groups were significantly decreased (p < 0.05) after periodontal treatments. Conclusions: The periodontal treatment improves the clinical parameters in both smokers and non-smokers. These results confirm that non-surgical periodontal therapy can reduce oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.
Efficiency of Low-Level Laser Therapy in Reducing Pain Induced by Orthodontic Forces
(MARY ANN LIEBERT, INC, 2012) Bicakci, Ali Altug; Kocoglu-Altan, Burcu; Toker, Hulya; Mutaf, Ilhan; Sumer, Zeynep
Objective: The aim of this study was to investigate the effect of low-level laser therapy (LLLT) on reducing post-adjustment orthodontic pain via evaluation of gingival crevicular fluid (GCF) composition changes at the level of prostaglandin-E-2 (PGE(2)) and visual analogue scale (VAS). Background data: LLLT has been found to be effective in pain relief. PGE(2) has the greatest impact on the process of pain signals and can be detected in GCF in order to investigate the response of dental and periodontal tissues in a biochemical manner. Materials and methods: Nineteen patients (11 females and 8 males; mean age 13.9 years) were included in this study. Maxillary first molars were banded and then a randomly selected first molar at one side was irradiated (lambda 820 nm; continuous wave; output power: 50 mW; focal spot: 0.0314 cm(2); exposure duration: 5 sec; power density: 1.59W/cm(2); energy dose: 0.25 J; energy density: 7.96 J/cm(2) for each shot), while the molar at the other side was served as placebo control. The GCF was collected from the gingival crevice of each molar to evaluate PGE(2) levels, before band placement, 1 and 24 h after laser irradiation. Pain intensity was analyzed at 5 min, 1 h, and 24 h after band placement by using VAS. Results: Although no difference was found in pain perception at 5 min and 1 h, significant reduction was observed with laser treatment 24 h after application (p < 0.05). The mean PGE(2) levels were significantly elevated in control group, whereas a gradual decrease occurred in laser group. The difference in PGE(2) levels at both 1 and 24 h were statistically significant between two groups (p < 0.05). Conclusions: The significant reductions in both pain intensity and PGE(2) levels revealed that LLLT was efficient in reducing orthodontic post-adjustment pain.
Evaluation of IL-1 beta, IL-1ra, and IL-10 levels and outcome of periodontal therapy in chronic periodontitis with familial Mediterranean fever
(SPRINGER HEIDELBERG, 2017) Bostanci, Vildan; Toker, Hulya; Senel, Soner; Poyraz, Omer; Akpinar, Aysun; Gorgun, Emine Pirim; Bakar, Olcay
This study aimed to examine the IL-1 beta, IL-1ra, and IL-10 cytokine levels in gingival crevicular fluid (GCF) and serum of familial Mediterranean fever (FMF) and chronic periodontitis (CP) patients, and their response to nonsurgical periodontal therapy. A total of 50 patients, 15 FMF patients with generalized chronic periodontitis (FMF-CP), 15 systemically healthy patients with generalized chronic periodontitis (CP), ten systemically and periodontal healthy controls (HC), and ten periodontally healthy FMF patients (FMF-HC) were enrolled in the study. The cytokine levels in GCF and serum were determined by ELISA. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks were recorded. The study indicated statistically significant healing of the clinical parameters in both FMF-CP and CP groups after periodontal treatment. GCF IL-1 beta levels at 6 weeks in FMF-CP group were significantly lower than the CP group (p < 0.05), and GCF IL-1ra levels were significantly decreased at 6 week in the FMF-CP group (p < 0.05). GCF IL-10 levels were significantly higher in the FMF-CP group than in the other groups at baseline and 6 weeks (p < 0.05). There were no significant differences in serum-IL-1 beta, IL-1ra, and IL-10 levels either FMF-CP or CP groups at baseline or 6 weeks (p > 0.05). The results of our study suggested that there was a positive correlation between gingival inflammation and serum cytokine levels in FMF patients and also colchicine treatment showed protective effects on GCF cytokine levels in FMF-CP group. Following treatment, GCF IL-1 beta and GCF IL-1ra levels were decreased in FMF-CP group. GCF IL-10 levels were increased in FMF-CP group compared to other groups. Also, the serum cytokine levels associated with periodontal inflammation in FMF patients.
The histopathological and morphometric investigation of the effects of systemically administered boric acid on alveolar bone loss in ligature-induced periodontitis in diabetic rats
(TAYLOR & FRANCIS LTD, 2014) Yuce, Hatice Balci; Toker, Hulya; Goze, Fahrettin
Objective. The purpose of this study was to evaluate the effects of systemically administered boric acid on alveolar bone loss, histopathological changes and oxidant/antioxidant status in ligature-induced periodontitis in diabetic rats. Materials and methods. Forty-four Wistar rats were divided into six experimental groups: (1) non-ligated (NL, n = 6) group, (2) ligature only (LO, n = 6) group, (3) Streptozotocin only (STZ, n = 8) group, (4) STZ and ligature (STZ+LO, n = 8) group, (5) STZ, ligature and systemic administration of 15 mg/kg/day boric acid for 15 days (BA15, n = 8) group and (6) STZ, ligature and systemic administration of 30 mg/kg/day boric acid for 15 days (BA30, n = 8) group. Diabetes mellitus was induced by 60 mg/kg streptozotocin. Silk ligatures were placed at the gingival margin of lower first molars of the mandibular quadrant. The study duration was 15 days after diabetes induction and the animals were sacrificed at the end of this period. Changes in alveolar bone levels were clinically measured and tissues were histopathologically examined. Serum total antioxidant status (TAS), total oxidant status (TOS), calcium (Ca) and magnesium (Mg) levels and oxidative stress index (OSI) were evaluated. Primary outcome was alveolar bone loss. Seconder outcome (osteoblast number) was also measured. Results. At the end of 15 days, the alveolar bone loss was significantly higher in the STZ+LO group compared to the other groups (p < 0.05). There was no significant difference in alveolar bone loss between the STZ+LO 15 mg/kg boric acid and STZ+LO 30 mg/kg boric acid groups (p > 0.05). Systemically administered boric acid significantly decreased alveolar bone loss compared to the STZ+LO group (p < 0.05). The osteoblast number in the BA30 group was significantly higher than those of the NL, STZ and STZ+LO groups (p < 0.05). Inflammatory cell infiltration was significantly higher in the STZ+LO group the other groups (p < 0.05). Serum TAS levels were significantly higher in the NL and LO groups than the other groups (p < 0.05). The differences in TOS levels were not found to be significant among all the groups (p > 0.05). The OSI values of the BA30 group were significantly lower than the STZ+LO group (p < 0.05). Also, the differences in serum calcium and magnesium levels were insignificant among the all groups (p > 0.05). Conclusion. Within the limits of this study, it can be suggested that BA, when administered systemically, may reduce alveolar bone loss in the diabetic rat model.
IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters
(SOCIEDADE BRASILEIRA DE PESQUISA ODONTOLOGICA, 2017) Gorgun, Emine Pirim; Toker, Hulya; Korkmaz, Ertan Mahir; Poyraz, Omer
Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin (IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid (GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). GAgP patients showed significant improvement on clinical parameters after periodontal therapy (p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levels were observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6 (-174) and IL-10 (-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6 (-174) GG genotype had higher levels of GCF IL-10 levels.
Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment
(WILEY-BLACKWELL, 2012) Toker, Hulya; Akpinar, Aysun; Aydin, Huseyin; Poyraz, Omer
Toker H, Akpinar A, Aydin H, Poyraz O. Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment. J Periodont Res 2012; 47: 572577. (C) 2012 John Wiley & Sons A/S Background and Objective: The aim of this study was to evaluate the impact of smoking on the relationship between interleukin-1 (IL-1 beta) and oxidation in patients with periodontitis and response to nonsurgical periodontal therapy. Material and Methods: Data were obtained from 30 patients with generalized chronic periodontitis (15 smokers and 15 nonsmokers) and from 10 periodontally healthy controls. IL-1 beta level, total oxidant status (TOS) and total antioxidant status (TAS) were recorded in gingival crevicular fluid. Probing depth, clinical attachment level, gingival and plaque indices and bleeding on probing were also measured. The gingival crevicular fluid and clinical parameters were recorded at baseline and 6 wk after periodontal treatment. Results: The study showed statistically significant improvement of clinical parameters in both smokers and nonsmokers after periodontal treatment. Moreover, the baseline IL-1 beta levels were significantly higher in smokers compared with nonsmokers (p < 0.05). After periodontal treatment, the IL-1 beta levels were significantly reduced in both smokers and nonsmokers (p < 0.05). There were no significant differences in TOS and TAS between periodontitis patients and healthy controls at baseline and 6 wk after periodontal treatment. The level of IL-1 beta in gingival crevicular fluid was positively correlated with TOS in both smokers and nonsmokers. Conclusions: Periodontal treatment improved the clinical parameters in both smokers and nonsmokers. The results confirm that periodontal therapy has an effect on IL-1 beta levels in gingival crevicular fluid, but not on TOS and TAS.
A ligneous periodontitis and conjunctival lesions in a patient with plasminogen deficiency
(MOSBY-ELSEVIER, 2007) Toker, Hulya; Toker, Mustafa Ilker; Goze, Fahrettin; Turgut, Mehmet; Yilmaz, Abdulkerim
Destructive membranous periodontal disease is a rare and poorly defined entity that is a part of a systemic disease due to accumulation of fibrin material. The disease is characterized by gingival enlargement and periodontal tissue destruction that leads to rapid bone loss despite treatment efforts. We present a case with ligneous periodontitis and conjunctivitis.
A morphometric and histopathologic evaluation of the effects of propolis on alveolar bone loss in experimental periodontitis in rats
(AMER ACAD PERIODONTOLOGY, 2008) Toker, Hulya; Ozan, Fatih; Ozer, Hatice; Ozdemir, Hakan; Eren, Kaya; Yeler, Hasan
Background: Propolis collected by honeybees from various plant sources is a resinous hive product possessing a broad spectrum of biologic activities. Propolis has been used extensively in the diet to improve health and prevent disease. The purpose of this study was to analyze the morphometric and histopathologic changes associated with experimental periodontitis in rats in response to the systemic administration of propolis. Methods: Forty Wistar rats were divided into four experimental groups: non-ligated (NL; IN = 10); ligature only (LO; N = 10); and systemic administration of ligature and propolis (100 mg/kg body weight per day [Pro100; N = 10] or 200 mg/kg body weight per day [Pro200; N = 10]). Silk ligatures were placed at the gingival margin of the lower first molars in both mandibular quadrants. The study duration was 11 days, and the animals were sacrificed at the end of this period. Changes in alveolar bone levels were clinically measured, and tissues were histopathologically examined to assess the differences among the study groups. Results: At the end of 11 days, alveolar bone loss was significantly higher in the LO group compared to the NL, Pro 100, and Pro200 groups (P<0.05). Osteoclast numbers in the LO group were significantly higher than those of the NL, Pro 100, and Pro200 groups (P<0.05). Both dosages of propolis significantly reduced the periodontitis-related bone loss, but the differences between the two propolis groups were not statistically significant (P>0.05). Conclusion: The findings of this study provide morphologic and histologic evidence that propolis, when administered systemically, prevents alveolar bone loss in the rat model.

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