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    Resveratrol-Loaded Silver Nanoparticles as an Effective Strategy for Targeted Breast Cancer Therapy
    (Wiley-V C H Verlag Gmbh, 2024) Topcu, Ege; Hepokur, Ceylan; Kariper, I. Afsin; Oksuz, Kerim Emre; Yaylim, Ilhan
    The AgNPs-Rsvl complex was investigated for its anti-cancer effects on human breast cancer cell lines. The UV-vis spectrophotometer was used to perform synthesized AgNPs-Rsvl binding analyses. The size and morphology of the AgNPs were determined by field emission gun-transmission electron microscopy (FEG-TEM). The surface chemical signatures of AgNPs nanocomposites were assessed using FT-IR spectroscopy, and the zeta potential (zeta potential) of various AgNPs colloids was measured using the Zetasizer Nano ZS analyzer. Cell proliferation was analyzed using the XTT method and cell apoptosis was analyzed using the AnnexinV-fluorescein isothiocyanate (FITC)/PI method. The effects of resveratrol were evaluated on human breast cancer cell lines (MDA-MB-231 and MCF-7). As a result of flow cytometry, a decrease in viability was observed in the MDA-MB-231 cells, and an increase in early apoptotic, late apoptotic, and necrotic values was observed. Resveratrol arrests the cell cycle in the G0/G1 phase but when used with AgNPs, the cell cycle arrests at the S phase. The combination of resveratrol and AgNPs exhibited a cytotoxic effect on cancer cells, with MDA-MB-231 breast cancer cells being the most sensitive to AgNPs-Rsvl. Resveratrol and AgNPs arrested the cell cycle and increased apoptosis, indicating their potential use in breast cancer treatment.