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    A determination of the main regulators of necroptosis in testicular tissue under different heat stresses
    (Springer, 2025) Tatar, Musa; Tufekci, Kiymet Kubra; Uslu, Sema
    Although minimal increases in testicular temperature can compromise spermatogenesis and lead to fertility-related problems, the basic mechanism involved in germ cell destruction as a response to heat stress is still unclear. However, necroptosis is known to regulate a number of physiological and pathological events. This study investigated the role of RIPK1/RIPK3 and MLKL, the main regulators of necroptosis, against different heat stresses in testis tissue. Forty-two Wistar albino rats were divided into seven groups: six experimental exposed to heat stress and one control. Heat stress was induced by causing the rats to swim for 30 min daily for 60 days in a water bath at temperatures of 39 degrees C and 43 degrees C. Testis tissues were collected while the animals were under anesthesia on the 1st, 7th, and 14th days after 60 days of heat application. The tissues were first fixed in Bouin's solution. After routine histological procedures, immunohistochemical staining was performed on one-half of the tissues using RIPK1/RIPK3 and MLKL primary antibodies on serially collected 5 mu m-thick sections. Immunoblotting analysis was performed on the other half. Analyses revealed an increase in the expression of RIPK1/RIPK3 and MLKL proteins, regulators of necroptosis, in both the 39 degrees C and 43 degrees C groups, although this was greater in the tissue exposed to 43 degrees C heat stress. These molecules were also especially affected by round and elongated spermatids, and reactivity was observed in Leydig cells. In conclusion, exposure to increased temperature may cause RIPK1/RIPK3 and MLKL-mediated cellular changes in the testis.
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    Expression of ADAMTS 1-4-8 and placental growth factor in ovary and oviduct during pregnancy in the first trimester
    (Wiley, 2023) Tatar, Musa; Tufekci, Kiymet Kubra; Uslu, Sema; Oner, Jale
    A Disintegrin and Metalloprotease Domains with Thrombospondins Motifs (ADAMTS), proteinases responsible for the destruction of extracellular matrix structures, have essential roles in the physiological and pathological processes of the female reproductive system, which is a dynamic structure. This study aimed to evaluate the immunoreactivity of placental growth factor (PLGF) and ADAMTS' (1, -4, and -8) in the ovary and oviduct during pregnancy in the first trimester. Our findings suggest a predominant role of ADAMTS-4 and ADAMTS-8 as proteoglycan-degrading enzymes from the ADAMTS-1 in the first trimester. As an angiogenic factor, PLGF showed more immunoreactivity than ADAMTS-1 in the ovary. This study provides the first evidence that ADAMTS-4 and ADAMTS-8 are more expressed in ovarian cells and follicles at different developmental stages during the first trimester of pregnancy than ADAMTS-1. Consequently, we suggest that ADAMTSs and PLGF act together and may exert specific effects on the formation, stabilisation, and function (or a combination thereof) of the matrix surrounding and protecting the follicles.