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Öğe Antitumoral effects of Allium sivasicum on breast cancer in vitro and in vivo(SPRINGER, 2013) Tepe, Bektas; Tuncer, Ersin; Saraydin, Serpil Unver; Ozer, Hatice; Sen, Metin; Karadayi, Kursat; Inan, Deniz Sahin; Karadayi, Sule; Polat, Zubeyde; Akpulat, Askin; Duman, Mustafa; Koksal, Binnur; Turan, MustafaThis work aims to investigate the antiproliferative properties of Allium sivasicum (AS) on breast cancer. AS extracts were studied for cytotoxicity against the breast cancer cell lines. In vitro apoptosis studies of breast cancer cells were performed by annexin V staining in flow cytometry analyses. AS showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in AS treated cell lines were higher than the untreated control cells. The expressions of caspase-7 protein and TUNEL positive cells were much higher for the rats treated by AS, compared with the untreated control group. The expressions of the Ki-67 decreased in treatment groups compared with the control group. In vivo studies showed that mean tumor volume inhibition ratio in AS treated group was 38 % compared with the untreated rats. These results indicate that A. sivasicum has antitumoral potential against breast cancer.Öğe Antitumoral Effects of Melissa officinalis on Breast Cancer in Vitro and in Vivo(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2012) Saraydin, Serpil Unver; Tuncer, Ersin; Tepe, Bektas; Karadayi, Sule; Ozer, Hatice; Sen, Metin; Karadayi, Kursat; Inan, Deniz; Elagoz, Sahande; Polat, Zubeyde; Duman, Mustafa; Turan, MustafaBackground: There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. Here we investigated the antiproliferative properties of Melissa officinalis (MO) from Turkey on breast cancer. Methods: MO extracts were studied for cytotoxicity against breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). In vitro apoptosis studies were performed by annexin V staining and flow cytometry analyses. Immunohistochemistry for Ki-67 and caspase 7 in the tumoral tissue sections of DMBA-induced mammary tumors in rats was also performed, along with TUNEL assays to detect apoptotic cells. In vivo anticancer activity testing was carried out with reference to inhibition of growth of DMBA induced mammary tumors in rats. Results: MO showed cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells. Expression of caspase-7 protein and TUNEL positive cells were much higher in rats treated by MO, compared with the untreated control group, while expression of Ki-67 was decreased. Furthermore, in vivo studies showed that mean tumor volume inhibition ratio in MO treated group was 40% compared with the untreated rats. Conclusion: These results indicated that MO extrcts have antitumoral potential against breast cancer.Öğe Antitumoral effects of Salvia absconditiflora Greuter & Burdet syn. Salvia cryptantha Montbret & Aucher ex Benth. on Breast cancer(NATL INST SCIENCE COMMUNICATION-NISCAIR, 2013) Ozer, Hatice; Altun, Ahmet; Saraydin, Serpil Unver; Soylu, Sinan; Goktas, Selcuk; Tuncer, Ersin; Inan, Deniz Sahin; Koksal, Binnur; Temiz, Tijen Kaya; Tepe, Bektas; Sen, Metin; Karadayi, Kursat; Turan, MustafaThis work aims to investigate the antiproliferative properties of Salvia cryptantha on breast cancer. Salvia cryptantha (SC) extracts were studied for cytotoxicity against the breast cancer cell lines. In-vitro apoptosis studies of breast cancer cells were performed by nnexin V staining in flow cytometry analyses Immunohistochemistry studies for Ki-67 and p16 in the tumoral tissue sections of Dimethyl Benzanthracene (DMBA) induced mammary tumor in rats were performed. In-vivo anticancer activity testing was carried out by inhibiting the growth of mammary tumor in rats. SC showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in SC treated cell lines were higher than the untreated control cells. The expression of the Ki-67 decreased in treatment groups compared with the control group. The expression of p16 protein was much higher for the rats treated by SC, compared with the untreated control group. In vivo studies showed that mean tumor volume inhibition ratio in SC treated group was 38 % compared with the untreated rats. These results indicate that Salvia cryptantha has antitumoral potential against breast cancer.Öğe Association of higher proliferation of Ki67 with unfavorable prognostic factors and shorter survival in breast cancer(AMER SOC CLINICAL ONCOLOGY, 2012) Kilickap, Saadettin; Kaya, Yalcin; Yucel, Birsen; Tuncer, Ersin; Elagoz, Sahande…Öğe Biocompatibility of fiber-reinforced composite (FRC) and woven-coated FRC: an in vivo study(Springer Heidelberg, 2023) Nalbantoglu, Ahmet Mert; Eren, Kaya; Yanik, Deniz; Toker, Hulya; Tuncer, ErsinObjectives To investigate biocompatibility and bone contact area of FRC and woven-coated FRC (FRC-C) in rats. Materials and methods Sixty rats were allocated to three groups: FRC (n=20), FRC-C (n=20), and control group (n=20). Subgroups were determined as 4th (n=10) and 12th weeks (n=10). The specimens were placed in the femur of rats. In the control group, the bone defects were left empty and sutured. Four and 12 weeks after implantation, the rats were sacrificed. Histopathological examinations were performed in a semi-quantitative manner. Twenty rats (n=20) were used for scanning electron microscopy (SEM) examination. Bone contact surfaces were calculated in SEM analysis. A chi-square test was performed to analyze the data. Results No statistical difference was detected between the 4th and 12th weeks in the quality of bone union. Quality of bone union was lower in FRC compared to the control group in the 4th week (p=0.012) and the 12th week (p=0.017). The periosteal reaction at the 12th week was lower in FRC than in the control group (p=0.021). Bone contact of FRC and FRC-C was 85.5% and 86.3%, respectively. Conclusions FRC and FRC-C were biocompatible and showed no inflammation. The woven coating did not increase the quality of bone union and bone contact area, while not reducing biocompatibility.Öğe Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study(DEOMED PUBL, ISTANBUL, 2017) Durmus, Kasim; Bora, Adem; Dogan, Mehtap; Ozer, Hatice; Tuncer, Ersin; Altuntas, Emine ElifObjective: The aim of the present pilot study was to evaluate the effects of a single local dose administration of humic acid on healing of subcondylar mandibular fractures in rats. Methods: In this study, a randomized experimental protocol was used. The study was conducted with 16 male Wistar-albino rats that were 16-18 weeks old. The rats (n=16) were randomly divided into two groups: Group HA received humic acid (0.3 cc/site, n=8) and Group C received no additional medical administration (control group, n=8). A full-thickness surgical osteotomy was performed in the subcondylar area. A single dose of humic acid (0.3 cc/site) was administered locally by spraying on the bone surfaces of the fracture line. Mandible was dissected on postoperative day 21. Then, fractured hemimandibles were obtained for histopathological examination. Results: The median score of bone fracture healing was 7.16 (range: 7 to 8) in the Group HA and 7.50 (range: 7 to 8) in the Group C. When the groups were compared in terms of bone healing scores, there was no statistical difference between the Group HA and the Group C (p>0.05). Conclusion: Results of this study showed that local administration of humic acid was not efficient for healing of bone fractures. However, we are of the opinion that it is required to conduct more comprehensive studies, including humic acid's different concentrations and administration manners, evaluating the effects of humic acid on tissue both histopathologically and in terms of inflammatory and proinflammatory cytokine levels.Öğe A case of spinal extradural angiolipoma(DRUNPP-SARAJEVO, 2012) Ozer, Hatice; Tuncer, Ersin; Gurelik, Mustafa; Oztoprak, Ibrahim; Egilmez, Reyhan; Ozum, UnalAngiolipoma is a benign tumor composed of mature adipocytes admixed with vascular elements. This article presents a case of spinal extradural angiolipoma in 52-year-old man, who underwent surgical treatment with preoperational diagnosis of schwannoma. This rare clinical entity must be considered in the differential diagnosis for any spinal epidural lesion.Öğe CD44, membrane type 1-matrix metalloproteinase (MTI-MMP) and KI-67 expression in astrocytic tumors(SPRINGER, 2007) Kivanc, Fugen; Egilmez, H. Reyhan; Tuncer, Ersin; Ozer, Hatice; Karadag, Ozen; Goze, O. Fahrettin…Öğe Chordoid Meningioma - A Case Report: Clinicopathological Features and Differential Diagnosis of an Uncommon Tumor(DE GRUYTER OPEN LTD, 2016) Ozer, Hatice; Tuncer, Ersin; Saray, Gonul; Gurelik, Mustafa; Oztoprak, Ibrahim; Egilmez, ReyhanMeningiomas are tumors that originate from the arachnoid cell and the majority are benign and grade I tumors according to World Health Organization. Chordoid meningioma is an uncommon variant of meningioma and corresponds to grade II tumor in the World Health Organization Classification of Tumors of the Nervous System 2007 because of its more aggressive behavior and increased likelihood of recurrence. A 75-year-old female was referred to the neurosurgery department complaining of headache, syncope, and seizure. Radiological examination revealed a mass lesion in the neighbourhood of the frontal lobe that destructed bone and was associated with peritumoral edema. The patient underwent surgery. The tumor was totally excised with the dura beneath. Histopathological examination showed that the tumor was composed of clusters and cords of small polygonal cells with fine chromatin and eosinophilic vacuolated cytoplasm embedded in a myxoid matrix, and also focal whorls of spindle-shaped cells. Two mitoses were seen in 10 high power fields. Vascular proliferation was observed in some tumoral areas. Bone invasion was present. Immunohistochemical analysis of the tumor cells revealed widespread strong membranous and cytoplasmic expression of epithelial membrane antigen. The Ki67 labeling index was 6-8%. All of these findings were consistent with a diagnosis of chordoid meningioma, the neoplasm was identified as grade II based on the World Health Organization Classification, 2007. In this report we present a case of chordoid meningioma without classical radiological findings of meningioma with areas of vascular proliferation that mimicked glial tumors at histopathologic examination.Öğe A common disease but rare presentation: branchial cyst located on tonsil(SPRINGER WIEN, 2015) Dogan, Mansur; Erkoc, Mustafa Fatih; Yuce, Salim; Seker, Kubra Yildiz; Uysal, Ismail Onder; Tuncer, Ersin…Öğe Comparison of inflammatory level and symptom level in cases having chronic rhinosinusitis(DERMAN MEDICAL PUBL, 2018) Dogan, Mansur; Tuncer, Ersin; Kol, Iclal Ozdemir; Uysal, Ismail Onder; Yuce, Salim; Muderris, SuphiAim: The aim of this study was to Investigate whether the endoscopic findings, corona! computed tomography data and the level of histopathological changes observed in tissue samples had any effects on the quality of life in chronic rhinosinusitis cases. Material and Method: In this prospective study, 34 patients diagnosed with chronic rhinosinusitis (Group 1) and 34 volunteer individuals having no chronic rhinosinusitis (Group 2) were included. Detailed anamnesis, endoscopic examination findings, quality of life in patients with chronic rhinosinusitis questionnaire scores and coronal paranasal computed tomography scores were recorded in the files of all the cases. Endoscopic evaluation findings were evaluated using the Lund-Kennedy scoring system, all the cases were given a chronic rhinosinusitis evaluation questionnaire, coronal paranasal computed tomography findings were scored using the Lund-MacKay system. Results: The average quality-of-life score, the average radiological score, the average endoscopic examination score, the histopathological evaluation of the surgical specimens, the average inflammatory score, the average fibrosis scores and the average osteoblastic-osteoclastic score was statistically different between two groups. When a correlation analysis was performed, the quality-of-life scores were found to have a negative correlation with the radiological scores, endoscopic scores. fibrosis scores and osteoblastic-osteoclastic activity scores in Group 1. And also radiological scores were positively correlated both with the endoscopic scores and the osteoblastic-osteoclastic activity scores and a positive correlation was found between the endoscopic scoring and the mucosal inflammation in Group 1. Discussion: The results of our study showed that the histopathological changes affected the quality of life negatively.Öğe The Effect of Topical Minoxidil Pretreatment on Nonsurgical Delay of Rat Cutaneous Flaps: Further Studies(SPRINGER, 2013) Gumus, Nazim; Odemis, Yusuf; Tuncer, Ersin; Yilmaz, SarperThe purpose of this study was to determine the effectiveness of topically applied minoxidil in the pharmacological delay phenomenon and to demonstrate the comparable microscopic and macroscopic changes between minoxidil-pretreated flaps and surgically delayed flaps. A modified version of the McFarlane flap was used. Group 1 rats, in which a caudally based dorsal skin flap was raised and sutured back, were the control group. In group II, minoxidil solution was spread over the marked skin flap area for 7 days. On the 7th day, a caudally based dorsal skin flap was elevated and then sutured back. Group III rats underwent a surgical delay procedure alone. On the 7th day after flap elevation, evaluation was done by histologic examination and calculation of the flap survival areas in all groups. The lowest flap survival rate appeared in group I and was statistically different from groups II and III. The mean surviving skin flap area in the minoxidil-pretreated group was significantly larger than that in the control group. After histologic evaluation, moderate angiogenesis was also detected in group II. We also found that surgical delay significantly reduced flap necrosis when compared to the minoxidil pretreatment group. According to our study, minoxidil may be considered an effective vasoactive agent for the stimulation of angiogenesis in rat cutaneous flaps and capable of achieving pharmacological delay and increasing flap survival. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.Öğe Effect of Topically Applied Minoxidil on the Survival of Rat Dorsal Skin Flap(SPRINGER, 2012) Gumus, Nazim; Odemis, Yusuf; Yilmaz, Sarper; Tuncer, ErsinFlap necrosis still is a challenging problem in reconstructive surgery that results in irreversible tissue loss. This study evaluated the effect of topically applied minoxidil on angiogenesis and survival of a caudally based dorsal rat skin flap. For this study, 24 male Wistar rats were randomly divided into three groups of eight each. A caudally based dorsal skin flap with the dimensions of 9 x 3 cm was raised. After elevation of the flaps, they were sutured back into their initial positions. In group 1 (control group), 1 ml of isotonic saline was applied topically to the flaps of all the animals for 14 days. In group 2, minoxidil solution was spread uniformly over the flap surface for 7 days after the flap elevation. In group 3, minoxidil solution was applied topically to the flap surface during a 14-day period. On day 7 after the flap elevation, the rats were killed. The average area of flap survival was determined for each rat. Subdermal vascular architecture and angiogenesis were evaluated under a light microscope after two full-thickness skin biopsy specimens had been obtained from the midline of the flaps. The lowest flap survival rate was observed in group 1, and no difference was observed between groups 1 and 2. Compared with groups 1 and 2, group 3 had a significantly increased percentage of flap survival (P < 0.05). Intense and moderate angiogenesis also was observed respectively at the proximal and distal areas of the flaps in group 3. The results of this experiment seem to show that the early effect of minoxidil is vasodilation and that prolonged use before flap elevation leads to angiogenesis, increasing flap viability. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.Öğe Efficacy of Chlorhexidine Gluconate During Surgery for Hydatid Cyst(SPRINGER, 2009) Topcu, Omer; Sumer, Zeynep; Tuncer, Ersin; Aydin, Cengiz; Koyuncu, AyhanDissemination of protoscolices-rich fluid during surgery for hydatid cyst disease is a major cause of recurrence. Instillation of a scolicidal agent into a hepatic hydatid cyst before opening is the most commonly employed measure to prevent this serious complication. In this clinical study, the efficacy of 0.04% chlorhexidine gluconate (Chx-Glu) during hydatid cyst surgery was tested, and early-term results were evaluated. A total of 30 consecutive patients with 45 liver cysts were studied. Only type I and type II cysts were included. Cysts were punctured, and fluid was aspirated. The viability of protoscolices in this fluid was determined. Chx-Glu 0.04% was instilled into the cyst cavity. After 5 minutes of exposure, the cyst fluid was reaspirated and evaluated for the viability of protoscolices. Imprints of the germinative membranes were determined for protoscolices viability. All protoscolices in 45 cysts evaluated were killed by 5 minutes of exposure to 0.04% Chx-Glu. The whole of the germinative membrane imprints contained dead protoscolices. Chx-Glu 0.04% did not cause any adverse effect on biliary tracts in communication with cysts. There was no recurrence during the 2-year follow-up period. There were also no mortality and no cavity-related complications. This study shows that intracystic injection of 0.04% Chx-Glu is an effective measure against the dissemination of viable protoscolices. In addition, Chx-Glu is the most convenient scolicidal agent as per the criteria defined by the World Health Organization. Therefore, it can be used safely during hydatid cyst surgery. However, there remains the need to perform advanced comparative clinical studies on the efficacy of Chx-Glu and other scolicidal agents.Öğe Exploring the Prognostic Role of Trop-2, CD47, and CD163 Expression Levels on Survival Outcomes in Patients with Triple-Negative Breast Cancer(MDPI, 2025) Yuceer, Ramazan Oguz; Aydin, Sedanur; Gelir, Iclal; Koc, Tulay; Tuncer, Ersin; Ucar, MahmutBackground: This study evaluated the prognostic impact of Trop-2, CD47, and CD163 expression on clinical outcomes in triple-negative breast cancer (TNBC) and investigated their interactions with tumor progression. Methods: A retrospective cohort of 92 patients with TNBC was analyzed. The expression scores for Trop-2, CD47, and CD163 were categorized as negative/low (0-3 points) or high (4-6 points). The primary endpoint was overall survival (OS). Results: The median age of the cohort was 50 years old. High Trop-2 expression was observed in 55.4% of the patients and was significantly associated with advanced disease stage (p < 0.001). High CD47 expression (44.6%) was correlated with advanced stage (p = 0.044), whereas high CD163 expression (45.7%) was associated with advanced stage (p = 0.021), absence of comorbidities (p = 0.022), and lower pT stage (p = 0.023). Moderate positive correlations were found between Trop-2 and CD47 (p = 0.022), Trop-2 and CD163 (p = 0.037), and CD47 and CD163 (p < 0.001), respectively. Kaplan-Meier survival analysis revealed that patients with low Trop-2 expression exhibited significantly prolonged OS (p = 0.021) and progression-free survival (PFS) (p = 0.026) compared to those with high Trop-2 expression. Univariate and multivariate analyses revealed significant associations between OS and PFS for Trop-2, lymphovascular invasion, and BRCA status. Conclusions: Trop-2 expression is a significant prognostic factor for TNBC and is correlated with worse outcomes. Although CD47 and CD163 showed trends for poorer prognosis, their significance was not confirmed. These findings offer promising prospects for future studies on combined antibody-drug conjugates (ADCs), as they may present opportunities to address multiple resistance mechanisms in the management of TNBC and enhance clinical outcomes.Öğe Expression of p16 Protein and Cyclin D1 in Periampullary Carcinomas(Federation Turkish Pathology Soc, 2011) Tuncer, Ersin; Turk, Nilay Sen; Arici, Sema; Duzcan, Suleyman Ender; Demirka, Nese CalliObjective: The majority of the tumors in periampullary region are pancreatic and ampulla of Vater carcinomas. The aim of this study was to compare histopathological features of ampulla of Vater carcinomas with pancreatic ductal adenocarcinomas and to determine diagnostic and predictive values of p16 protein and cyclin D1 expression. Material and Method: Tissue samples from pancreatic ductal adenocarcinomas and ampulla of Vater carcinomas were obtained from 31 patients who underwent pancreticoduodenectomy for periampullary carcinoma. The study group was composed of 12 women and 19 men. Their median age was found to be 62.32 years (range 26-85 years). The parameters analyzed in the study included lymph node metastases, perineural invasion, differentiation, duodenal invasion, grade of intraepithelial neoplasia and p16 and cyclinD1 expression in tumoral and peritumoral pancreatic tissues. Results: In both tumor groups, the loss of p16 protein expression was significantly correlated with perineural invasion (p= 0.0001). Perineural invasion was more frequent in the pancreatic ductal adenocarcinoma group than the ampulla of Vater carcinoma group (p= 0,01). When desmoplasia and lymphoplasmacytic stromal infiltration were examined, desmoplastic reaction was significantly higher in pancreatic ductal adenocarcinomas than ampulla of Vater carcinomas (p= 0.01). No significant difference was observed between tumor groups for Cyclin D1 (p> 0.05). Conclusion: The results suggest that loss of p16 protein expression may be a sign for poor prognosis in periampullary cancers that is correlated mainly with perineural invasion. Desmoplastic stromal reaction may be a distinctive feature for pancreatic ductal adenocarcinoma compared with ampulla of Vater carcinoma.Öğe Higher Ki67 Expression is Associates With Unfavorable Prognostic Factors and Shorter Survival in Breast Cancer(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2014) Kilickap, Saadettin; Kaya, Yalcin; Yucel, Birsen; Tuncer, Ersin; Babacan, Nalan Akgul; Elagoz, SahandeBackground: The prognostic value of the Ki67 expression level is yet unclear in breast cancer. The aim of this study was to investigate the association between Ki67 expression levels and prognostic factors such as grade, Her2 and hormone receptor expression status in breast cancers. Materials and Methods: Clinical and pathological features of the patients with breast cancer were retreived from the hospital records. Results: In this study, 163 patients with breast cancer were analyzed, with a mean age of 53.4 +/- 12.2 years. Median Ki67 positivity was 20% and Ki67-high tumors were significantly associated with high grade (p<0.001), lymphovascular invasion (p=0.001), estrogen receptor (ER) negativity (p=0.035), Her2 positivity (p=0.001), advanced stage (p<0.001) and lymph node positivity (p<0.003). Lower Ki67 levels were significantly associated with longer median relapse-free and overall survival compared to those of higher Ki67 levels. Conclusions: High Ki67 expression is associated with ER negativity, Her2 positivity, higher grade and axillary lymph node involvement in breast cancers. The level of Ki67 expression is a prognostic factor predicting relapse-free and overall survival in breast cancer patients.Öğe HISTOPATHOLOGICAL ASSESSMENT OF AXILLARY LYMPH NODES WITH MULTIPLE LEVEL SECTIONS IN BREAST CARCINOMA(Aves, 2011) Ozer, Hatice; Tuncer, Ersin; Elagoz, Sahande; Koyuncu, Ayhan; Inal, SevincPurpose: This study was planned to evaluate whether assessing five histological sections separated by 25 mu m detects significantly more tumor metastases in axillary lymph nodes. Methods: The study was a retrospective study cmposed of 56 mastectomy specimens. An extra four levels were cut from all blocks containing non-metastatic lymph nodes of each individual case. The original section was then counted as the first level. All the sections were then examined for the presence of tumor metastases within lymph nodes at all five levels. Results: Twenty seven N0, 10 N1, 10 N2 and 9 N3 cases were examined. Three hundred and twelve paraffin blocks containing nonmetastatic lymph nodes were cut, resulting in an extra 1248 sections, and eight hundred and forty two lymph nodes were examined. Thirteen extra metastases were discovered in nodes at new levels. One of these extra metastases occurred in newly appearing lymph nodes that had not been present in the level 1. None of the cases in which extra lymph node metastases were changed in respect to the tumor stage. Conclusion: Although extra metastases detected in this study did not increase the stages, histopathologic evaluation with multiple level sections may provide more significant prognostic knowledge compared to evaluation with one level section. But concerning the labour intensity and cost effectivity, the benefit that would be obtained from that procedure may be controversial.Öğe Histopathological evaluation of the effect of locally administered strontium on healing time in mandibular fractures: An experimental study(WROCLAW MEDICAL UNIV, 2017) Durmus, Kasim; Turgut, Nergiz Hacer; Dogan, Mehtap; Tuncer, Ersin; Ozer, Hatice; Altuntas, Emine Elif; Akyol, MelihBackground. Mandibular fractures are the most common facial fractures. They can be treated by conservative techniques or by surgery. The authors hypothesized that the application of a single local dose of strontium chloride would accelerate the healing of subcondylar mandibular fractures, shorten the recovery time and prevent complications. Objectives. The aim of the present pilot study was to evaluate the effects of a single local dose of strontium chloride on the healing of subcondylar mandibular fractures in rats. Material and methods. This randomized experimental study was carried out on 24 male Wistar albino rats. The rats were randomly divided into 3 groups: experimental group 1, receiving 3% strontium chloride; experimental group 2, receiving 5% strontium chloride; and the control group. A full thickness surgical osteotomy was created in the subcondylar area. A single dose of strontium solution (0.3 cc/site) was administered locally by injection on the bone surfaces of the fracture line created. Nothing was administered to the control group. The mandibles were dissected on postoperative day 21. The fractured hemimandibles were submitted to histopathological examination. Results. The median bone fracture healing score was 9 (range: 7-9) in experimental group 1; 8 (range: 7-10) in experimental group 2; and 7.50 (range: 7-8) in the control group. When the groups were compared in terms of bone healing scores, there was a statistically significant difference between experimental group 1 and the control group (p < 0.05). Conclusions. This study is the first to show that local strontium may have positive effects on the healing of subcondylar mandibular fractures. In the authors' opinion, 3% strontium was beneficial for accelerating facial skeleton consolidation and bone regeneration in rat subcondylar mandibular fractures. This treatment procedure may be combined with closed fracture treatment or a conservative approach.Öğe Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors(BIOMED CENTRAL LTD, 2012) Ozer, Hatice; Yenicesu, Goncalmir; Arici, Sema; Cetin, Meral; Tuncer, Ersin; Cetin, AliBackground: In many tumors including ovarian cancer, cell proliferation and apoptosis are important in pathogenesis and there are many alterations in most of the genes related to the cell cycle. This study was designed to evaluate immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, and bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in typing of benign, borderline, and malignant serous and mucinous ovarian tumors. Methods: Total of 68 ovarian tumors, 25 benign [13 (19.1%) serous and 12 (17.6%) mucinous], 16 borderline [9 (13.2%) serous and 7(10.3%) mucinous], and 27 malignant ovarian tumors [24 (35.3%) serous and 3 (4.4%) mucinous tumors] were included in the study. Immunohistochemical expression of p53, p21, bax, bcl-2, telomerase, c-kit, and metallothionein were evaluated. Results: When all 68 cases were evaluated as benign, borderline, and malignant ovarian tumors without considering histopathological subtypes, the p53, p21, bax and metallothionein showed significantly higher staining scores in the borderline and malignant ones (p < 0.05). After evaluation of all 68 cases, the serous tumors showed significantly higher staining scores of p53, p21, c-kit, and metallothionein compared to the mucinous ones (p < 0.05). For differentiation of benign and borderline and malignant tumors combined, p53 was not used because all benign tumors has no staining, and p21, bax, and metallothionein was determined the significant predictors for borderline and malignant tumors combined (p < 0.05). For differentiation of borderline and malignant tumors, only p53 was determined the significant predictor for malignant tumors (p < 0.05). Conclusions: In conclusion, p53, p21, bax, c-kit, and metallothionein may be helpful for the typing of ovarian tumors as benign, borderline and malignant or serous and mucinous. p53, p21, bax, c-kit, and metallothionein may have different roles in the pathogenesis of ovarian tumor types. p53 and metallothionein may be helpful in the typing of borderline and malignant ovarian tumors. The immunohistochemical staining with bcl-2 and telomerase may not provide meaningful contribution for the typing of ovarian tumors.
