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Öğe Assessment of therapeutic effect of human choriogonadotropin in a chemical cystitis model(ELSEVIER TAIWAN, 2017) Tanik, Serhat; Zengin, Kursad; Albayrak, Sebahattin; Gurel, Abdullah; Atar, Muhittin; Sahin, Sevinc; Tuzcu, Nevin; Tuzcu, Mehmet; Imamoglu, Muhammet Abdurrahim; Gurdal, MesutIn this study, female rats induced with chemical cystitis were administered the hormone human choriogonadotropin (HCG), and it was aimed to reveal the usefulness of HCG in the treatment of interstitial cystitis/bladder pain syndrome. The materials for this study were 32 Wistar albino female rats. The study groups were formed as follows: the cystitis group (Group 1), the cystitis + HCG protection group (Group 2), the cystitis + HCG treatment group (Group 3), and the control group (Group 4), with eight rats in each group. In this study, blood and urine samples were taken from the rats, they were euthanized, and their bladders were removed for glutathione, malondialdehyde, tumor necrosis factor alpha, and interferon gamma measurements. It was observed that tissue damage in Group 2 was lower than that in the other two groups. Glutathione levels in Groups 2 and 4 were significantly higher than in Groups 1 and 3 (p = 0.01). Malondialdehyde levels of Groups 2 and 4 were significantly lower than the values in Groups 1 and 3 (p < 0.001). When the cystitis groups were compared in terms of their interferon gamma and tumor necrosis factor alpha levels, the lowest interferon gamma and tumor necrosis factor alpha levels were detected in Group 3. It was found that HCG has positive effects on experimental cystitis in rats. This study revealed that HCG should be researched as a therapeutic agent and formed a step for studies to be carried out on this subject. Copyright (C) 2017, Kaohsiung Medical University. Published by Elsevier Taiwan LLC.Öğe Determination of Apoptosis, Necroptosis and Autophagy Markers by Real-time PCR in Naturally Infected Pneumonic Pasteurellosis caused by Pasteurella multocida and Mannheimia haemolytica in Cattle(Univ Agriculture, Fac Veterinary Science, 2024) Akcakavak, Gokhan; Karatas, Ozhan; Tuzcu, Nevin; Tuzcu, MehmetPneumonic pasteurellosis (PP) is defined as one of the pivotal infectious diseases caused by Pasteurella multocida and Mannheimia haemolytica . This study aimed to determine the levels of Bcl-2-associated protein X (Bax), B-cell lymphoma 2 (Bcl2), caspase-3, autophagy related-5 (Atg5), beclin-1 and receptor interacting protein3 ( RIP3) in lung tissues with naturally infected PP caused by P. multocida and M. haemolytica , and to reveal their effects on the pathogenesis of P. multocida and M. haemolytica pneumonia. The material of the study consisted of 150 fibrinous pneumonia/pleuropneumonia and 10 healthy lung tissue samples. Relevant samples were examined by histopathological, immunohistochemical and real-time PCR methods. Immunohistochemically, 23 (15.3%) were positive for P. multocida, and 17 (11.3%) were positive for M. haemolytica . Subsequently, the processes of apoptosis, autophagy and necroptosis for P. multocida and M. haemolytica were evaluated by real-time PCR. P. multocida pneumonia increased Bax, Caspase-3, Atg5, Beclin-1, and RIP3 gene expressions (4.2, 3.8, 2.9, 2.1, 2.8-fold, respectively), whereas Bcl-2 gene expression was decreased (0.22-fold). While Bax, Caspase-3, Atg5, Beclin-1, and RIP3 gene expressions were increased in M. haemolytica pneumonia (2.3, 1.9, 1.7, 1.2, 4.2-fold, respectively), it was observed that Bcl-2 gene expression was reduced (0.52-fold). The results obtained in the study revealed the importance of necroptosis, apoptosis and autophagy processes in the pathogenesis of PP caused by P. multocida and M. haemolytica and contributed to the literature. In addition, we found that the processes of apoptosis and autophagy play a more active role in PP caused by P. multocida , and the process of necroptosis plays a more active role in PP caused by M. haemolytica.Öğe Determination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method(2024) Akçakavak, Gökhan; Karataş, Özhan; Cifci, Aysenur Tural; Dağar, Osman; Doğan, Osman; Tuzcu, MehmetWhite muscle disease (WMD) is also known as Stiff Lamb Disease or Nutritional Muscular Dystrophy. Selenium and/or Vitamin E deficiency constitutes the etiology of the disease. This study aimed to immunohistochemically evaluate local protein expressions of Nuclear factor kappa B (NF-kB), Insulin-like growth factor-1 (IGF-1) and Microtubule-related protein 1A/1B-light chain 3 beta (LC3B) in WMD. The material of the study consisted of 15 WMD, and 6 healthy lamb heart samples. The heart tissues of the autopsied lambs were subjected to routine tissue processing and paraffin blocks were obtained. Then, it was stained with Hematoxylin-Eosin and immunohistochemical methods. Control group lambs had normal macroscopic appearance. Macroscopically, hyaline degeneration and zenker’s necrosis, calcification areas were observed in WMD tissues. Microscopically, degenerative and necrotic muscle fibers, calcification areas, fibrosis, mononuclear cell infiltrates and macrophage infiltrates were detected in WMD heart tissues. Immunohistochemically, significant increases were detected in IGF-1 (p<0.001), LC3B (p<0.001) and NF-kB (p<0.05) in the WMD group compared to the control group. Immunoreactivity in the relevant primers was detected commonly in degenerative and necrotic muscle fibers. In addition, occasional immunoreactivity was observed in the relevant primers in inflammatory cell infiltrates. In conclusion, NF-kB, IGF-1 and LC3B protein expressions were evaluated immunohistochemically for the first time in lambs with WMD. Our findings show that IGF-1 and LC3B proteins are highly expressed in heart tissue in WMD. Additionally, it is possible to say that IGF-1 and LC3B can be used in the diagnosis of WMD.Öğe Diagnostic value of serum procalcitonin, neopterin, and gamma interferon in neonatal calves with septicemic colibacillosis(SAGE PUBLICATIONS INC, 2016) Ercan, Nazli; Tuzcu, Nevin; Basbug, Onur; Tuzcu, Mehmet; Alim, AhmetThe objective of our study was to compare the serum concentrations of procalcitonin (PCT), neopterin, tumor necrosis factor alpha (TNF-), prostaglandin E-2, malondialdehyde, interleukin 8, and gamma interferon (IFN-) in neonatal calves with septicemic colibacillosis (n = 15) with healthy controls (n = 15). Septicemic colibacillosis was diagnosed based on clinical signs and the isolation of Escherichia coli from heparinized blood in clinically suspected cases. PCT, neopterin, TNF- (p < 0.05), and IFN- concentrations in calves with septicemic colibacillosis were significantly higher than in the healthy controls (p < 0.01). Serum PCT concentrations were similar to 4 times higher in the calves with septicemic colibacillosis than in the healthy calves, suggesting that PCT could be a useful marker of septicemic colibacillosis in neonatal calves.Öğe Köpeklerde canine distemper virüs enfeksiyonunun klinik, patolojik ve real-time RT-PCR ile teşhisi(2021) Tuzcu, Nevin; Tuzcu, Mehmet; Akçakavak, Gökhan; Başbuğ, Onur; Kurul, MehmetKlinik olarak canine distemper virüs (CDV) teşhisi konulan köpeklerde enfeksiyonun hem sürüntü örneklerinde ve hem de postmortem alınan doku örneklerinde hızlı teşhisine yönelik real-time RT-PCR metodu çalışılarak, viral nükleik asit tespitine dayalı real-time RT-PCR metodu ile histopatolojik yöntemin karşılaştırması amaçlanmıştır. Çalışmanın materyalini, ateş, gözyaşı akıntısı ile solunum, sindirim ve sinir sistemi enfeksiyonlarına ait klinik bulgulara sahip, CDV enfeksiyonu klinik tanısı ile tedaviye başlanmış, ancak tedaviye yanıt vermeden ölen köpeklerden histopatolojik bulgulara göre CDV teşhisi konulmuş köpeklere ait beyin, beyincik, karaciğer, akciğer, dalak, böbrek, taban yastığı ve kan ile tonsil, burun ve gözden alınan sürüntü örnekleri oluşturdu. Histopatolojik incelemelerde CDV enfeksiyonu için karakteristik sayılan intrasitoplazmik ve intranükleer inklüzyon cisimciği belirlenen köpeklerden nekropsi sırasında alınmış sürüntü örneklerinde viral nükleik asit varlığı incelendiğinde tonsillerden alınan sürüntülerin tamamında, burun ve konjunktivadan alınan örneklerin iki tanesinde CDV nükleik asit kopyalarının belirlendiği, kan örneklerinde ise viral nükleik asit belirlenemediği görüldü. Köpeklerde görülen klinik bulgular hastalığı düşündürse de kesin teşhis için kullanılan virolojik ve histopatolojik incelemeler uzun zaman almaktadır. Bu durum viral nükleik asitlerin çoğaltılması esasına dayalı PCR yöntemlerini ön plana çıkarmakta, PCR yöntemleri içerisinde de uygulayıcı hatasını minimize eden, hızlı sonuç veren ve virüs sayısının tahmin edilebildiği real-time RT-PCR uygulamalarının önemini artırmaktadır.Öğe Pathological, cytological, microbiological and molecular investigations of pneumonia caused by Pasteurella multocida and Mannheimia haemolytica(2020) Tuzcu, Mehmet; Tuzcu, Nevin; Başbuğ, OnurAim: In cattle breeding, pneumonia caused by Pasteurella multocida andMannheimia haemolytica is very important due to the economic losses caused. In this study, it was aimed to reveal cytological findings in pneumonicbovine lungs and nasal swaps due to P. multocida and M. haemolytica determined by microbiological methods and real time PCR.Materials and Methods: In this study, bacteriological culture, histopathological, cytological and real time PCR techniques were used in pneumonic bovine lungs. Cytological findings were evaluated in cases of pneumonia whoseetiological agent was identified as M.haemolytica and P.multocida by realtime PCR and were compared with other pneumonia typesResults: Cytological findings were recorded in smear samples prepared fromnasal swaps and lung samples. Pneumonia caused by P. multocida and M.haemolytica was detected in 23 cases (9.87%) out of 233 cattle with signsof pneumonia. P.multocida and M.haemolytica were determined to occur inabout 51.11% of the animals with fibrinous pneumonia. During the cytological examination of these cases, neutrophils were seen increased in numbercompared to the other types of pneumonia. In addition to neutrophils, thenumber of lymphocytes and ciliated epithelial cells was also significantly increased in these cases compared to the other fibrinous pneumonia cases inwhich P.multocida ve M.haemolytica was not detected.Conclusion: The results of this study showed that P. multocida and M. haemolytica cause a fibrinous type of pneumonia and constitute an importantportion of cattle respiratory diseases. Clinically, cytology results may be evaluated for the typing of pneumonia.Öğe The protective effects of sildenafil in acute lung injury in a rat model of severe scald burn: A biochemical and histopathological study(ELSEVIER SCI LTD, 2013) Gokakin, Ali Kagan; Deveci, Koksal; Kurt, Atilla; Karakus, Boran Cihat; Duger, Cevdet; Tuzcu, Mehmet; Topcu, OmerSevere burn induces biochemical mediators such as reactive oxygen species that leads to lipid peroxidation which may have a key role in formation of acute lung injury (ALI). Sildenafil is a selective and potent inhibitor of cyclic guanosine monophosphate specific phosphodiesterase-5. Sildenafil preserves alveolar growth, angiogenesis, reduces inflammation and airway reactivity. The purpose of the present study was to evaluate the effects of different dosages of sildenafil in ALI due to severe scald burn in rats. Twenty-four rats were subjected to 30% total body surface area severe scald injury and were randomly divided into three equal groups as follow: control, 10 and 20 mg/kg sildenafil groups. Levels of malondialdehyde (MDA), activities of glutathione peroxidase (Gpx), catalase (Cat), total oxidative stress (TOS), and total antioxidative capacity (TAG) were measured in both tissues and serums. Oxidative stress index (OSI) was calculated. A semi-quantitative scoring system was used for the evaluation of histopatological findings. Sildenafil increased Gpx, Cat, TAG and decreased MDA, TOS and OSI. Sildenafil decreased inflammation scores in lungs. Our results reveal that sildenafil is protective against scald burn related ALI by decreasing oxidative stress and inflammation and the dosage of 10 mg/kg could be apparently better than 20 mg/kg. (C) 2013 Elsevier Ltd and ISBI. All rights reserved.Öğe Strontium Chloride: Can It Be a New Treatment Option for Ulcerative Colitis?(HINDAWI LTD, 2014) Topal, Firdevs; Yonem, Ozlem; Tuzcu, Nevin; Tuzcu, Mehmet; Ataseven, Hilmi; Akyol, AndmelihBackground/Aims. Patients with ulcerative colitis still need effective therapy without major side effects. It has been found that strontium can suppress NF kappa B activation induced by TNF-alpha. This opens a gate to a new anti-TNF agent which is cheap and can be given orally. We for the first time aimed to investigate the effect of strontium chloride (SrCl2) on inflammation in experimental colitis. Methods. Thirty female Wistar albino rats were divided into 5 groups each containing 6 rats. The rats in groups 1 and 2 served as the healthy control and colitis group, respectively. The rats in groups 3, 4, and 5 had colitis and received 40 mg/kg SrCl2, 160 mg/kg SrCl2, and 1 mg/kg prednisolone by oral gavage, respectively. The rats were sacrificed for histological evaluation and determination of serum neopterin, TNF-alpha, and IFN-gamma levels. Results. The neopterin, TNF-alpha and IFN gamma levels of group 2 was significantly higher than the other groups. The neopterin, TNF-alpha, and IFN-gamma levels of controls and other treatment groups were comparable. There were a significant difference in macroscopic and microscopic healing between group 2 and other groups histologically. But there was not a significant difference within treatment receiving groups. Conclusion. SrCl 2 had comparable therapeutic efficiency with prednisolone.Öğe Tarantula cubensis alcohol extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats(University of Benin, 2024) Akcakavak, Gokhan; Celik, Zeynep; Karatas, Ozhan; Dogan, Osman; Ozdemir, Ozgur; Tuzcu, MehmetPurpose: To evaluate the effect of a combination of Tarantula cubensis alcohol extract (TCAE) and capecitabine (CAP) in the treatment of azoxymethane (AOM)-induced colorectal cancer (CRC). Methods: Forty-two Wistar albino rats were divided into 7 groups with 6 rats in each group. The groups consisted of Control (C), Control+TCAE (C-TCAE), Control+CAP (C-CAP), Cancer control (CC), Cancer+TCAE (CC-TCAE), Cancer+CAP (CC-CAP) and Cancer+CAP+TCAE (CC-CAP+TCAE). To induce CRC, AOM (15 mg/kg) was administered to rats subcutaneously (sc) twice at a one-week interval to all the groups except control. From the 15th week, TCAE (0.2 mL/rat sc) was administered to CC-TCAE group every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered by gavage to CC-CAP group for 4 weeks. In CC-CAP+TCAE group, TCAE (0.2 mL/rat sc) was administered every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered gavage for 4 weeks. Animals were treated for 18 weeks. Aberrant crypt foci (ACF) were evaluated histopathologically among CC, CC-TCAE, CC-CAP, and CC-CAP+TCAE groups. ?-catenin, CD15, Proliferating Cell Nuclear Antigen (PCNA), and Nuclear Factor kappa B (NF-?B) expression levels were immunohistochemically compared among all groups. Results: Histopathologically, ACF scores were significantly increased in CC group, while a significant decrease in the relevant scores (p < 0.001) was observed in CC-CAP and CC-CAP+TCAE treatment groups, and the lowest scores were in CC-CAP+TCAE group. Immunohistochemically, in CC group, ?-catenin, Nuclear Factor kappa B (NF-?B), Proliferating Cell Nuclear Antigen (PCNA) and CD15 expressions were highly irregular. CC-CAP and CC-CAP+TCAE groups had significantly reduced expressions (p < 0.001), and the lowest expressions were in CC-CAP+TCAE group. Conclusion: The combined use of TCAE and CAP in treatment of CRC has a synergistic effect and increases the anticancer efficacy of TCAE, and CAP. More studies at the molecular level are needed in the future to demonstrate the clinical benefit of TCAE supplementation during the treatment of CRC with CAP. © 2024 The authors. This work is licensed under the Creative Commons Attribution 4.0 International License.Öğe The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: A biochemical and histopathological study(Turkish Association of Trauma and Emergency Surgery, 2014) Gökakin, Ali Kağan; Atabey, Mustafa; Deveci, Koksal; Sancakdar, Enver; Tuzcu, Mehmet; Duger, Cevdet; Topcu, OmerBackground: Severe burn induces systemic inflammation and reactive oxygen species leading to lipid peroxidation which may play role in remote organs injury. Sildenafil is a selective and potent inhibitor of cyclic guanosine monophosphate specific phosphodiesterase- 5. Sildenafil reduces oxidative stress and inflammation in distant organs. The aim of the present study was to evaluate the effects of different dosages of sildenafil in remote organs injury.; Methods: A total of thirty-two rats were randomly divided into four equal groups. The groups were designated as follows: Sham, Control, 10, and T20 mg/kg sildenafil treatment groups. Levels of malondialdehyde (MDA), vascular endothelial growth factor (VEGF), VEGF receptor (Flt-1), activities of glutathione peroxidase (Gpx), levels of total antioxidative capacity (TAC), and total oxidant status (TOS) were measured in both tissues and serum, and a semi-quantitative scoring system was used for the evaluation of histopathological findings.; Results: Sildenafil increased levels of Gpx, and Flt-1, and decreased MDA and VEGF levels in tissues. Sildenafil also increased serum levels of TAC and Flt-1 and decreased TOS, OSI, and VEGF.; Conclusion: Sildenafil decreased inflammation scores in remote organs in histopathological evaluation. It has protective effects in severe burn-related remote organ injuries by decreasing oxidative stress and inflammation. © 2014, TJTES.
