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Öğe Alternative plants to be used in natural dyeing on wool yarn fibers(TAYLOR & FRANCIS INC, 2019) Kaynar, Hulya; Ucar, EsraIn this study, 28 plants were collected from nature and the extracts were obtained with and without mordants from these plants. The wool yarns (3.5 N m natural wool carpet yarns obtained from the natural fibers 60 s, 23.3 mu m, 10(-4) cm) were colored by each of these extracts and the light and abrasion fastness of obtained colored substances of various colors were determined. While the plant showing the highest light fastness was walnut tree leaf (Juglans regia), the plant showing the highest abrasion fastness was determined as quince leaf (Cydonia oblonga). On the other hand, while the ferric sulfate as a mordant provided the highest light fastness, the highest abrasion fastness was obtained from the experimental group to which mordant was not applied.Öğe Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Guclu, Gulsen; Ergul, Merve; Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Akpulat, Huseyin AskinThe Inula aucheriana, of the Asteraceae family, is widespread in Turkey. The aim of this study was to investigate different biological properties and thereby reveal the pharmacological potential of this plant, which is already known to be used in the treatment of various diseases,. Following the preparation of 80% ethanol extract from I. aucheriana, qualitative and quantitative methods were used to investigate the chemical composition (Q-TOF analysis), antioxidant (spectrophotometric analysis), inhibitory enzyme (Ellman's method), antimicrobial (MIC concentration value), and anti-cancer (XTT analysis) activities. The results showed that 80% ethanol extract from I. aucheriana was a potent antioxidant, with anti-cancer and enzyme inhibitor effects. In the chemical composition analysis, the primary compound of the extract was determined to be luteolin (32.55%). I. aucheriana extract was seen to have AChE and BChE inhibition, , and when compared with the reference drug, the extract was determined to have an inhibitory effect an enzyme called alpha-glucosidase. Besides, relatively high tyrosinase enzyme inhibition was also detected. The extract significantly showed antiproliferative activity on the MDA-MB-231 cells at 0.0625 mg/mL and higher concentrations,for 24 hours in a a dose-dependent manner.This study is the first to evaluate enzyme inhibitory effect and antioxidant activity of I. aucheriana.Öğe Biological activities of different plant species belonging to the Asteraceae family(Pamukkale University, 2023) Guclu, Gulsen; Inanir, Merve; Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Uskutoğlu, Tansu; Senkal, Belgin CosgeAchillea biebersteinii and Anthemis tinctoria, which are widely distributed species of the Asteraceae family, are used in folk medicine in the form of herbal tea or extract in the treatment of many diseases. The aim of this study was to investigate the chemical content, antimicrobial, antioxidant, enzyme inhibitor activities and cytotoxic effects of 80% ethanol extract of these two species and make a comparative analysis. In accordance with the data obtained, the major component of A. biebersteinii was determined as Cyclododecane (14.47%), while that of A. tinctoria was determined as Phytol (23.15%). A. biebersteinii, which showed moderate activity in terms of antimicrobial activity, produced more active inhibition than A. tinctoria did. Both plants showed high levels of antioxidant activity. The total phenol and total flavonoid contents of A. tinctoria were higher than those of A. biebersteinii. It was determined that there was no significant activity when the extracts were compared with galanthamine, which is the reference drug in terms of enzyme inhibitory activity. When the in vitro anticancer activity of human breast cancer cell line was examined, it was determined that A. tinctoria had a cytotoxic effect at high concentrations (IC50;0.82mg/mL), and A. biebersteinii showed strong cytotoxicity at all concentrations (IC50;<0.0625mg/mL). These two plants of the same family were evaluated in terms of many different biological parameters and it was revealed that A. biebersteinii was more active than A. tinctoria. However, in vivo studies are needed to determine whether these plants can be used as phytotherapeutic agents. © 2023 The Author(s).Öğe Comparative study of in vitro antioxidant, acetylcholinesterase and butyrylcholinesterase activity of alfalfa (Medicago sativa L.) collected during different growth stages(DE GRUYTER POLAND SP ZOO, 2018) Eruygur, Nuraniye; Dincel, Burak; Dincel, Nazire Gulsah Kutuk; Ucar, EsraMedicago sp. are often consumed as vegetable, salad and herbal tea as a form of fresh leaves and herbs. It is also very important in animal feeding, because it contains high percentage of protein and some important phytochemicals. In addition, traditionally, the leaves or other parts of the plant were used for treatment and preventing of kidney disorders, osteoporosis, anemia, diabetes, ulcer, coronary diseases, some cancers, and menopausal symptoms. In previous reports, it has been demonstrated that this plant has scavenging activity of the free radicals, which are involve in the development of the hypertension, Lschemia, neurodegenerative and rheumatoid diseases. In this study, the antioxidant, enzyme inhibitory activities of M. sativa L. collected in two growing period and phenolic substances that may be responsible for these activities were investigated. In this way, the beneficial effects of the plant will he revealed and a scientific work will be provided for the evaluation of the pharmaceutical and food industry.Öğe DETERMINATION OF BIOLOGICAL ACTIVITY OF ETHANOL EXTRACTS OF DATE-PLUM (Diospyros lotus) FRUITS AND SEEDS GROWING IN TURKEY(Univ Life Sciences Lublin, 2024) Ucar, EsraThis study was carried out to evaluate quality criteria such as chemical composition and antioxidant and antibacterial activity values in 80% ethanol extracts of date-plum ( Diospyros lotus ) fruits and seeds. In addition, the macro and microelement concentrations of fruits and seeds were also investigated. According to the data obtained, when fruit and seed were compared, it was determined that the seed (81.72%) contained more components than the fruit (79.4%), and the antioxidant activity of the seed was also higher. While the main component of the seed was Methyl hydrogen disulfide with 43.21%, the main component of the fruit was 5-Hydroxymethylfurfural with 24.2%. As a result of antimicrobial activity tests, neither seeds nor fruits have antimicrobial activity. At the same time, the nutritional content values of the seeds and fruits of this plant were analyzed and evaluated. When the data obtained are evaluated in terms of both macro and micronutrients, it has been observed that the nutritional content values of the fruit (K (3.63%), P (0.68 %), Cu (24.31%), Zn (10.49 mg kg -1 ) and Mn (25.29 mg kg -1 ) for fruits) are higher than the seeds. In conclusion, the findings from the evaluation of Diospyros lotus fruit and seeds in this study highlight the richness in chemical composition and high antioxidant activity of the seeds, as well as the nutritional superiority of the fruit. Therefore, further research to better understand and harness the potential health benefits of this plant could contribute to a deeper understanding of this field.Öğe Determination of biological activity of Tragopogon porrifolius and Polygonum cognatum consumed intensively by people in Sivas(Elsevier, 2020) Eruygur, Nuraniye; Ucar, Esra; Atas, Mehmet; Ergul, Merve; Ergul, Mustafa; Sozmen, FazliObjective: This study was aimed to investigate the in vitro antioxidant, antimicrobial, cytotoxicity, and enzyme inhibition activities of Tragopogon porrifolius and Polygonum cognatum which are naturally grown and consumed intensively by people in Sivas, Turkey. Methods: Plant materials were extracted with aqueous ethanol by maceration method. The components of the extracts were determined using the Gas Chromatography Mass Spectrometry. Antimicrobial, cytotoxic and enzyme inhibition activities of the extracts were investigated by micro dilution, XTT assay and 96-micro-well plate methods, respectively. The antioxidant activity evaluated using the DPPH radical scavenging, thiobarbituric acid and reducing power methods. The total phenol and total flavonoid content was also examined. Results: GC-MS analysis revealed the presence of 31 compounds inP. cognatum extract and 29 compounds in T. porrifolius extract. According to the results, T. porrifolius extract showed high level of antioxidant activity in comparison to P. Cognatum extract. T. porrifolius exhibited higher alpha-glucosidase inhibitory activity, and both extract showed strong alpha-amylase inhibition activity compared to reference drug acarbose. T. porrifolius and P. cognatum ethanolic extracts exhibited antimicrobial activity in the concentration range of 0.039-2.5 mg/ml. Both extracts also exhibited significant anticancer effect on MDA-MB-231 breast cancer cells. The IC50 values of T. porrifolius and P. cognatum extracts in MDA-MB-231 cells were determined as 0.0625 mg/mL and 0.053 mg/mL, respectively. Conclusion: Our findings demonstrated that T. porrifolius and P. cognatum ethanolic extracts have promising effect on antioxidant, antimicrobial and cytotoxic activity as well as enzyme inhibition activity, and hence further studies required to identify specific compounds responsible for these activities.Öğe Determination of inhibitory activities of enzymes, related to Alzheimer's disease and diabetes mellitus of plane tree (Platanus orientalis L.) extracts and their antioxidant, antimicrobial and anticancer activities(C M B ASSOC, 2018) Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Ergul, Merve; Ergul, Mustafa; Sozmen, FazliPlane tree (Platanus orientalis L.) leaves have been employed for centuries in various countries due to their pharmacological value. Therefore, determination of the biological activity of the leaves is of interest. The aim of the study was to evaluate the inhibitory effects against Alzheimer's disease-related enzymes Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE), diabetes mellitus related enzymes alpha-glucosidase and alpha-amylase. The antioxidant, anticancer, and antimicrobial activities of the leaves were also studied. According to the results, both water and methanol extracts of P. orientalis demonstrated more alpha-glucosidase and alpha-amylase inhibition activity than the antidiabetic drug-acarbose at the same concentration level. In addition, extracts showed good inhibition activity against AChE and BuChE. Significant results were obtained regarding antioxidant, anticancer, and antimicrobial activities. These results are very promising especially for the improvement of pharmaceutical formulations to treat various diseases such as age-related diseases, cancer, diabetes etc. and it is necessary to conduct further experiments.Öğe Effect of Different Nitrogen Doses on Dry Matter Ratio, Chlorophyll and Macro/Micro Nutrient Content in Sweet Herb (Stevia rebaudiana Bertoni)(TAYLOR & FRANCIS INC, 2017) Ucar, Esra; Ozyigit, Yasar; Demirbas, Ahmet; Guven, Dilek Yasin; Turgut, KenanWith this study, the effect of different nitrogen (N) doses applied to sweet herb (0, 50, 100, 150 and 200N kg ha(-1)) on the macro/micro nutrient element content, dry matter ratio and on the chlorophyll amount in the plant leaves is examined. The research was carried out in the research and trial field of Faculty of Agriculture Agronomy department, Akdeniz University, during the 2012-2013 growing season under field conditions for 2years with four replications according to the randomized block trial design. Chlorophyll amounts were determined and the average was obtained with three readings from each leaf by a chlorophyll meter when the plants were in the beginning of flowering. Post-harvest macro and micro nutrient elements of the sweet herb were also investigated. According to the obtained data, while the highest chlorophyll and N amounts in the leaf for both the first and second years were determined as 46.97 SPAD (Minolta Corp, NJ, USA) and 1.34N% 44.9 SPAD, 1.42N%, respectively, under 200kg ha(-1) N application, the lowest chlorophyll and N amounts were determined in the control group. In addition, N application in different doses for both years increased the phosphorus (P) and potassium (K) contents and increased the microelement contents, especially in the second year. At the same time, a correlation between the chlorophyll content in the plant leaves and the N amount was determined and a linear increase was observed in the chlorophyll amount with the increased green part.Öğe The effect of different nitrogen levels on yield and quality of stevia (Stevia rebaudiana bert.)(TAYLOR & FRANCIS INC, 2018) Ucar, Esra; Turgut, Kenan; Ozyigit, Yasar; Ozek, Temel; Ozek, GulmiraIn the present work, the efficiency of different nitrogen doses (0, 50, 100, 150, and 200kg ha(-1)) on growth, yield, and quality of stevia (Stevia rebaudiana Bert.) was investigated in 2011-2013. The study was conducted in Antalya located in the Mediterranean Region of Turkey. Terra rossa type soil (LVx, FAO) characteristics of the experimental field were clay loam, with high amounts of lime (33,9%) and slightly alkaline (pH 7.7). The experiment was carried out in randomized block design with four replications. All the results were summarized as mean of three years. The highest fresh and dry biomass yields (26.75 t ha(-1) and 7.5 ha(-1), respectively) were obtained from 150kg ha(-1) N dose and followed by 100kg ha(-1) N dose (26.29 t ha(-1) and 7.24 ha(-1), respectively). Whereas the highest fresh and dry leaf yields (13.27 t ha(-1) and 3.82 t ha(-1), respectively) were realized in 100kg ha(-1) N dose. Actually, all nitrogen doses gave higher biomass and leaf yields compared to the control. On the hand, major steviol glycosides (stevioside and rebaudioside A) in the leaf were not influenced by nitrogen levels. In conclusion, 100kg ha(-1) N dose was found to be suitable for cultivation of stevia under field conditions.Öğe The Effect of the Plant Age and Growth Period on the Nutritional Substance, Chlorophyll and Steviol Glycoside Rates in Stevia (Stevia Rebaudiana Bertoni) Leaves(TAYLOR & FRANCIS INC, 2018) Ucar, Esra; Ozyigit, Yasar; Eruygur, Nuraniye; Guven, Dilek; Yur, Suleyman; Turgut, Kenan; Ozek, Temel; Kutuk, NursahStevia (Stevia rebaudiana Bertoni) is a perennial and subshrub herb of the Asteraceae family, which originates from South America. Stevia has long been used as a sweetener and herbal medicine throughout the world. The most important feature of stevia is its sweetness with zero calories. This natural feature of Stevia is of utmost importance to everyone, especially those with diabetes and those who diet. In this study, samples were taken from Stevia rebaudiana plants of different ages (2, 3 and 4 years) in different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October) and an evaluation was made on the nutritional content values, the chlorophyll and sugar rates found in these samples according to the age and growth period of the plant. It was determined that the age of the plant and the harvesting period had an effect on the macro-micro nutritional elements, and chlorophyll and steviol glycoside rates contained in the stevia plant. In addition, as a result of correlation analysis, significant correlations were determined between the parameters measured in the plant. The results of the study demonstrated that in the cultivation of stevia, the best values were obtained from plants that were 3 years old and harvested in July.Öğe The Effects of different nitrogen doses on antioxidant and antimicrobial activity of Stevia (Stevia rebaudiana Bert.)(C M B ASSOC, 2018) Atas, Mehmet; Eruygur, Nuraniye; Ucar, Esra; Ozyigit, Yasar; Turgut, KenanIn this study, the efficiency of the different nitrogen doses (0, 5, 10, 15 and 20 kg ha(-1)) on biological activity levels (antioxidant and antimicrobial activity) of Stevia rebaudiana Bert. was investigated. In addition, methanol extracts were obtained by maceration method from different doses of fertilizer applied stevia. The components in methanol extracts of plants were determined by gas chromatography-mass spectrometry (GC-MS) method. Antimicrobial activities of stevia extracts were investigated by microdilution method. The antioxidant activity evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric thiocyanate (FTC), thiobarbituric acid (TBA), reducing power, total phenol content (TPC), and total flavonoid content (TFC) methods. According to the results, the fertilizer doses effects on antimicrobial activity of stevia were not made much difference. But in antioxidant activity, there were some variations in the activity-dependent on fertilizer amount.Öğe Estimation of in vitro antioxidant and antimicrobial activity of methanol extracts from Stevia(Parlar Scientific Publications, 2019) Eruygur, Nuraniye; Atas, Mehmet; Ucar, Esra; Ozyigit, Yasar; Turgut, KenanIn this study, the efficiency of the plant age and growth period on antioxidant and antimicrobial activity of stevia (Stevia rebaudiana Bert.) was investigated. Samples were taken from S. rebaudiana plants of three different ages (2, 3 and 4 years old) and four different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October). Extracts of sweet herb (S. rebaudiana) were obtained by maceration method with methanol as solvent. The experiments were carried out in completely randomized design with three replications. According to results, the highest antioxidant activity (metal chelating activity of methanol extract from stevia leaf; DPPH Free Radical Scavenging Activity of methanol extract from stevia leaves) was determined from respectively No.2 sample (3-year-old plant harvested in July) and No.1 sample (2-year-old plant harvested in July). The highest antimicrobial and antifungal activity for Escherichia coli and Staphylococcus aureus was determined from No.9, No.10, and No.11 samples (in the order of 3-year-old plant harvested in September, 3-year-old plant harvested in October, and 4-year-old plant harvested in November). All of the stevia samples harvested in different age and different periods were showing antimicrobial effect against Pseudomonas aeruginosa, E. faecalis and Candida albicans. © 2019 Parlar Scientific Publications. All rights reserved.Öğe ESTIMATION OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACTS FROM STEVIA(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2019) Eruygur, Nuraniye; Atas, Mehmet; Ucar, Esra; Ozyigit, Yasar; Turgut, KenanIn this study, the efficiency of the plant age and growth period on antioxidant and antimicrobial activity of stevia (Stevia rebaudiana Bert.) was investigated. Samples were taken from S. rebaudiana plants of three different ages (2, 3 and 4 years old) and four different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October). Extracts of sweet herb (S. rebaudiana) were obtained by maceration method with methanol as solvent. The experiments were carried out in completely randomized design with three replications. According to results, the highest antioxidant activity (metal chelating activity of methanol extract from stevia leaf; DPPH Free Radical Scavenging Activity of methanol extract from stevia leaves) was determined from respectively No.2 sample (3 year-old plant harvested in July) and No.1 sample (2-year-old plant harvested in July). The highest antimicrobial and antifungal activity for Escherichia coli and Staphylococcus aureus was determined from No.9, No.10, and No.11 samples (in the order of 3-year-old plant harvested in September, 3-year old plant harvested in October, and 4-year-old plant harvested in November). All of the stevia samples harvested in different age and different periods were showing antimicrobial effect against Pseudomonas aeruginosa, E. faecalis and Candida albicans.Öğe Evaluation of antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of Hypericum heterophyllum vent., an endemic species in Turkey's Flora(Elsevier, 2024) Eruygur, Nuraniye; Ucar, Esra; Tuzun, Burak; Atas, Mehmet; Inanir, Merve; Demirbas, Ahmet; Bal, HalilThe aim of this study was determined the antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of H. heterophyllum (endemic species in the Flora of Turkey) extracts. H. heterophyllum methanolic extract was used in this study. H. heterophyllum plants collected at the beginning of flowering and during full flowering. P content was determined with calorimetrically in spectrophotometer. K, CA, Mg, Fe, Mn, Zn and Cu was determined with Atomic Absorption and N concentration were determined according to the Kjeldahl distillation method. H. heterophyllum (endemic species in the Flora of Turkey) extract was evaluated for its antioxidant (DPPH, ABTS, total phenol and flavonoid content), antimicrobial (four bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus) and two Candida species (Candida albicans and Candida tropicalis)), enzyme inhibition activity (alpha-glucosidase, alpha-amylase, acetylcholinesterase, butyrylcholinesterase, tyrosinase), and cell viability (XTT assay) capacity. Extracts from the beginning of flowering period showed stronger biological activity. It has been determined that the extracts have antioxidant, antibacterial (against Staphylococcus aureus) anticancer activity (on breast cancer cells), and inhibition effects of BChE and alphaglucosidase. It has been determined that the extracts obtained from H. heterophylum have important pharmacological activities, and the plant exhibits higher activity in the BF period. Hypericum heterophyllum Vent. The activities of the chemicals contained in it against various proteins that are alpha-gly enzyme (PDB ID: 1R47), AChE enzyme (PDB ID: 4M0E), BChE enzyme (PDB ID: 5NN0), alpha-Amylase (PDB ID: 3BAJ), breast cancer protein (PDB ID: 1A52 and 1JNX), were compared. ADME/T analysis of molecules with high activity was performed.Öğe Evaluation of Various Biological Activities of Endemic Sideritis libanotica Extracts(Horticulture and Forestry Society from Transylvania, 2019) Atas, Mehmet; Eruygur, Nuraniye; Sozmen, Fazli; Ergul, Mustafa; Ergul, Merve; Akpulat, Huseyin Askin; Ucar, EsraIn recent years, using of plants to develop combine therapies for treatment of certain diseases such as cancer, Alzheimer's disease, and diabetes mellitus is extremely plausible approach. Perhaps, the definitive treatment of some of these diseases is hidden in the complex content of a plant in nature. Thus, in the present study, we decided to determine the biological activities of methanol and water extracts of the endemic Sideritis libanotica. To the best of our knowledge, the present study is the first investigation on antioxidant, enzyme inhibitory activity, antimicrobial activity, and in vitro cytotoxicity of S. libanotica. According to results, while the methanol extract shows better anti-cholinesterase, ?-glucosidase, and ?-amylase inhibition activity and cytotoxicity than the water extract, the tyrosinase inhibitory activity of the water extract was found to be better than the methanol extract. This study provides valuable information on how the biological activity of endemic S. libanotica changes in different solvent extractions such as water and methanol. © 2019, Horticulture and Forestry Society from Transylvania. All rights reserved.Öğe In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium(SPRINGER, 2019) Eruygur, Nuraniye; Ucar, Esra; Akpulat, Huseyin Askin; Shahsavari, Keyhan; Safavi, Seyed Mehdi; Kahrizi, DanialHypericum lydium Boiss. is a perennial plant of the Hypericaceae family, which has been used in particular to treat depression. The aim of this study was to determine in vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (-glucosidase/-amylase) and Tyrosinase inhibitor activity of methanol and water extracts of H. lydium. Also, gene expression has been evaluated in the shoot and root by microarray technology. So, in general, the purpose of this study is to study the active molecules such as antioxidant, antimicrobial, antidiabetic, enzymes and genes in the plant, which is the first to be reported. The experiments were conducted in a completely randomized design with three replications. In addition, gene expression was compared in the shoot and root parts. Expression profiling was carried out by microarrays. According to the results, the highest chemical components were determined in methanol extract rather than water extract. There was a difference between the obtained components. While the highest antioxidant activity was determined from the methanol extract of plant herbs for DPPH Free Radical Scavenging Activity, antioxidant activity was the same in both methanol and water extracts using the ABTS method. The methanol extract demonstrated stronger anticholinesterase (AChE and BChE) and -amylase inhibition activity. This study was complemented by the detection of antioxidant activity and some enzyme inhibition activity in the methanol extract. Microarray showed 10,784 genes had significantly different expression in root and shoot. There was a positive effect of methanol extract in respect of different activities compared to the water extract. Gene expression showed that the number of expressed genes in the root was greater than the shoot.Öğe IN VITRO ANTIOXIDANT, ANTIMICROBIAL, ANTICANCER ACTIVITIES ASSESSMENT OF THYMUS PECTINATUS, SCREENING OF ENZYME INHIBITORY(Parlar Scientific Publications (P S P), 2021) Ergul, Merve; Guclu, Gulsen; Atas, Mehmet; Cetin, Metin Durmus; Eruygur, Nuraniye; Ucar, Esra; Akpulat, Huseyin AskinAccording to the data obtained in this study, Thymus pectinatus has antioxidant and enzyme inhibitory activities while has not showed antimicrobial activities. The results of the current study provide valuable information, showing that the major component of the water extract of T. pectinatus is 1,3-Propanediol, 2 -methyl-, dipropanoate (59.34 %). This is the first investigation of the antioxidant and enzyme inhibitory activity of T. pectinatus. The results showed that the water extract had high antioxidant, anti-amylase, and anticancer effects, and a higher total phenolic content. Therefore, further phytochemical and bioactivity-guided isolation of T. pectinatus water extract could be carried out to identify the active compounds.Öğe In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts(Turkish Pharmacists Assoc, 2019) Ergul, Mustafa; Ergul, Merve; Eruygur, Nuraniye; Atas, Mehmet; Ucar, EsraObjectives: The present study aimed to investigate the inhibitory activities of enzymes related to diabetes mellitus and Alzheimer's disease of the methanol and water extracts of Ficus carica leaf extracts. The bioactive compounds and anticancer, antioxidant, and antimicrobial effects of the extracts were also investigated. Materials and Methods: The bioactive compounds in the extracts were determined by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6 sulphonic acid) (ABTS) radical scavenging, total phenol and flavonoid content, ferric reducing power, and iron chelating method. The anticancer, anticholinesterase, and antimicrobial effects were investigated using the XTT assay, Ellman method, and microdilution, respectively. Results: Our results showed that between the water and methanol extracts there was a difference in terms of chemical composition. The antioxidant results suggested that both extracts have strong antioxidant activity. Similarly, both extracts showed strong alpha-glucosidase and alpha-amylase inhibition activity, while the water extract had higher inhibition activity than the methanol extract against acetylcholinesterase and butyrylcholinesterase. The methanol extract of F. carica exhibited significant anticancer activity on MDA-MB-231 cells and showed moderate antimicrobial activities against Escherichia coli and Staphylococcus aureus. Conclusion: Our results suggest that F. carica leaves could be a valuable source for developing a promising therapeutic agent in cancer, diabetes, and Alzheimer's disease.Öğe IN-VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES AND VARIOUS ENZYME INHIBITORY ACTIVITIES OF ETHANOL EXTRACTS FROM DIFFERENT ORGANS OF CONSOLIDA REGALIS(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2018) Ucar, EsraIn this study in-vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (alpha-glukosidase/alpha-amylase) and tyrosinase inhibitor activity of ethanol (80%) extract of different organs of Consolida regalis were determined. The stem extract showed stronger anticholinesterase (AChE and BChE) inhibition activity than the extracts from the other parts. While the strong alpha-glucosidase inhibition was determined in the leaf extract, the highest alpha-amylase inhibition activity has been shown in the stem extract. It was worth to pointing out that the ethanol extract of stem of the Consolida regalis demonstrated more tyrosinase inhibition activity than the reference compound and also showed higher -amylase inhibition activity than the antidiabetic drug-acarbose at the same concentration level. So these results may be helpful for development of novel pharmaceutical formulations to treat various diseases such as diabetes.Öğe P 24: Clone Selection for High Quality Types of Oregano (Origanum dubium Boiss.)(JULIUS KUHN-INST, 2016) Turgut, Kenan; Tutuncu, Begum; Ozyigit, Yasar; Ucar, Esra; Marthe, F; Riegler, HOriganum dubium Boiss. is one of the economically important wild oregano species and it is intensely collected from the natural flora of Antalya in Turkey. Carvacrol chemotype of Origanum dubium is used mainly for essential oil production due to its high essential oil content. In this study, chemical diversity of Origanum dubium was investigated in order to develop new cultivars with improved essential oil yield and carvacrol content using clone selection method under cultivated condition. Essential oils obtained by hydrodistillation of the aerial parts of Origanum dubium were analysed by GC-MS and 24 different components were identified. In these genotypes, carvacrol was the major component and followed by p-cymene, gamma-terpinene, myrcene and alpha-thujene. Among the selected genotypes, essential oil yields were varied between 5.0% to 14.0%; carvacrol rates were varied between 73.76% to 88.21%; and p-cymene rates were varied between 3.16% to 9.10%.
