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Öğe Adverse effects of oxytetracycline and enrofloxacin on the fertility of Saanen bucks(Springer, 2021) Yucel, Ufuk Mercan; Kosal, Volkan; Kara, Mikail; Taspinar, Filiz; Uslu, Baris AtalayThis study aimed to determine the adverse effects of oxytetracycline and enrofloxacin application on the fertility of Saanen bucks. For this purpose, twenty-four bucks were divided into three groups. Group I (control group) received only 5 ml of 0.9% NaCl for 7 days, group II was given a single dose of 20 mg/kg oxytetracycline and group III was given at a dose of 2.5 mg/kg per day for 7 days intramuscularly. Serum and semen samples were collected from the bucks at post-treatment 1, 3, 5, 7, and 9 days and examined spermatological parameters (quantity, motility, density, abnormal sperm ratio, and live-dead sperm ratio), serum testosterone levels (with ELISA) and sperm DNA parameters (with Comet assay). The results showed no change in sperm volume, abnormal sperm rate, and dead-live sperm ratio in group II and III following oxytetracycline and enrofloxacin administration. However, a decrease in sperm density, sperm motility, mass activity, and testosterone levels, and an increase in sperm DNA damage were detected. These spermatological parameters (density, motility, mass activity) and testosterone levels were less decreased and sperm DNA damage was less increased in group II than group III. The greater damage in group III may be attributed to the longer duration of enrofloxacin administration compared to oxytetracycline and the effect of enrofloxacin on DNA. The results obtained from this study suggest that usage of oxytetracycline and especially enrofloxacin should be restricted and antibiotics with fewer side effects on sperm should be preferred in Saanen bucks during the reproduction period.Öğe An Insight Into Veterinary Students' Perceptions on the use of 3D-Printed Bone Biomodels in Anatomy Learning(Univ Ljubljana, 2023) Kocyigit, Alper; Ari, Hasan Huseyin; Uslu, Baris AtalayToday, conventional teaching methods are losing their effectiveness at transferring knowledge and skills, prompting the presentation of alternative strategies that hold more promise. One of the innovative alternative education materials in veterinary anatomy education is the models produced on three-dimensional (3D) printers. The subject of this study is 4 different bone biomodels 3D modeled and printed with reference to cadaver-derived bones. In the study, a total of 298 students were asked to evaluate these biomodels in terms of their similarity to the reference bones. According to the survey, 75.5% of the students stated that their biomodel resembled the reference bones. In addition, 64.8% of these students stated that the use of biomodels can be efficient in learning the skeletal system. These outcomes showed that a sample from each of the 4 main bone types could be replicated on a 3D printer with an acceptable similarity ratio. Based on student opinions about these four different biomodels, we think that 3d printed biomodels deserve to be evaluated as an alternative in anatomy education.Öğe Effect of SpermVital® technology on conception rate in repeat breeder multiparous dairy cows: preliminary results(Tubitak Scientific & Technological Research Council Turkey, 2019) Kocyigit, Alper; Uslu, Baris Atalay; Sendag, Sait; Treupel, Elisabeth; Wehrend, AxelThe aim of this study was to investigate the effect of SV technology on conception rates in repeat breeder multiparous dairy cows. Seventy-nine multiparous Holstein cows from a private dairy farm were used in the study. These animals were cows that had failed to conceive from at least 3 regularly spaced services (repeat breeders). Estrus cycles of the cows were synchronized by 2 injections of the PG analogue, administered 11 days apart. GnRH was applied 48 h after the second injection of PG. Twenty-four h after this administration, the animals were randomly divided into 2 groups, control and SV. The animals in the control group (n = 28) were inseminated with standard processed semen, and the cows in the SV group (n = 51) were inseminated with SV (R) technology processed semen. A lower pregnancy rate (35.5%) was determined in the control group than in the SV (47.1%) group. The difference between pregnancy rates in the groups was statistically significant (P < 0.05). We are at too early a stage to say that SV (R) Technology can fully respond to the deficiencies in herd management. This work may also lead to future studies into the use of more animal material.Öğe Effects of long-term release GnRH agonist "deslorelin" on testicular HSP expression, accessory sex glands and testicular functions in adult male rats(ELSEVIER SCIENCE INC, 2019) Eski, Funda; Cetin, Nebi; Uslu, Sema; Uslu, Baris Atalay; Sendag, Sait; Yoruk, Mecit; Naseer, Zahid; Wehrend, Axel; Shakeel, MuhammadThe objective of the present was to determine the effect of long-term release GnRH agonists "deslorelin" on suppression and restoration of testicular and accessory sex glands functions, and expression of HSP in testes of adult male rats. A group of twenty-eight male rats and fifty-six female rats were kept for eleven months. The male rats were subdivided into treatment (n = 18; deslorelin, an analogue of GnRH, 4.7 mg, S.C; six months) and control (n = 10; untreated), and the adult female rats were introduced with either treatment or control male rats at the 2nd, 6th and 11th months post implant insertion. At 6th month of deslorelin implants insertion, six male rats from treatment and five rats from control group were sacrificed. The remaining (twelve treatment and five control) male rats were sacrificed at 11 months. The testicular dimension were measured monthly in both treatment and control rats. The blood samples were collected for testosterone and HSP70 antibody, whereas, the testes and accessory glands were isolated for histological examination at each sacrificial time. The results showed that testicular dimension were significantly lesser in treatment group until 9 months post treatment. HSP70 protein expression was negligible at 6 months in treatment group but its intensity increased in spermatids 11 months of treatment similar to control group. Significantly lower testosterone concentrations with poor semen quality, and smaller litter size were observed in treatment group. The histological picture of accessory sex glands and seminiferous tubules shown a variable integrity in treatment group than control at 6 months implant insertion. In conclusion, the subcutaneous application of 4.7 mg of the GnRH-analogue deslorelin represents a practicable, like in the female rats, method to suppress testicular, accessory sex glands functions, testicular HSP expression and fertility in male rats. Moreover, the suppressive effects of deslorelin, continued until 11th months after removal of the implant. (C) 2019 Elsevier Inc. All rights reserved.Öğe Evaluation of hematological parameters, oxidative stress, Iron and unsaturated Iron binding capacity levels in Kangal shepherd dogs, before and after orchiectomy(Univ Zulia, Facultad Ciencias Veterinarias, 2024) Yurdakul, Ibrahim; Uslu, Baris AtalayGonadectomy is a widely used method in reproductive health management, and behavior disorder therapies of domestic animals. Anemia and its development, one of the complications of surgical castration, which has been done so much recently in shelter dogs and dogs kept at home, has been investigated in male Kangal shepherd dogs. In castrated male Kangal shepherd dogs was to investigate the relationship between gonadectomy and oxidant and antioxidant levels, Iron (Fe) concentrations, unsaturated iron binding capacity (UIBC) and some blood parameters (RBC, HGB, HCT, MCV, RDW, MCHC). Blood samples were taken from 20 adult Kangal dogs brought to a clinic before castration (Day 0) and on the 1st, 3rd and 7th days. Some biochemical analyzes and blood parameters were evaluated in the blood samples taken. For this purpose, serum total oxidant- antioxidant capacity (TAC-TOC), Iron (Fe) concentrations, unsaturated iron binding capacity (UIBC) and some blood parameters (RBC, HGB, HCT, MCV, RDW, MCHC) were examined. In this study, it was evaluated the relationship between gonadectomy and oxidant-antioxidant capacity and Fe metabolism at some serum parameters in male dogs. After the orchidectomy operation, a remarkable decrease in clinical and statistical blood parameters was observed. As a result of this study, in the analysis of blood parameters, a severe picture of anemia was observed. It was determined an important role in erythropoiesis, with orchidectomy. The statistical difference in blood parameters (P<0.05) was indicative of this.Öğe Impact of Trolox Supplementation on the Cryopreservation of Honamli Buck Semen(Mary Ann Liebert, Inc, 2025) Gungor, Sukru; Inanc, Muhammed Enes; Uslu, Baris Atalay; Burca, Ahmet Burak; Ata, AyhanCryopreservation of buck semen is essential in animal breeding but often damages sperm viability and integrity. The Honamli breed, a hardy Turkish goat, can benefit from improved freezing techniques using antioxidants such as Trolox (T). This study explores the effects of varying T concentrations on Honamli buck semen, assessing parameters such as motility, viability, and membrane integrity to enhance post-thaw quality. Findings support T's potential to improve semen extender formulations for preserving Honamli genetics.This study aims to freeze Honamli buck semen with T and to evaluate in vitro spermatological parameters. Three Honamli bucks, aged 2-3 years, were used in the study. Semen was collected from the bucks and mixed after removing seminal plasma. The mixed semen was diluted with a tris egg yolk extender containing three different concentrations of T (0.25 mM, 0.5 mM, and 1 mM) and control (0 mM). The diluted semen was equilibrated for 2 hours at +4 degrees and subjected to cryopreservation in liquid nitrogen vapor (-120 degrees C for 12 minutes) and frozen. After thawing (37 degrees C water bath for 30 seconds), the groups were evaluated at flow cytometric analysis for viability (SYBR/propidium iodide [PI]), plasma membrane acrosome integrity (FITC-PNA/PI), and mitochondrial membrane potential (JC-1), plasma membrane integrity (hypo-osmotic swelling test), microscopic evaluations for motility and morphological integrity (abnormal spermatozoa rate). The 0.5 T and 0.25 T groups showed significant improvements in motility compared with the control group (p < 0.05). The control group had the lowest plasma membrane integrity (p < 0.05). The highest morphological integrity was observed in the T groups compared with the control group (p < 0.05). In conclusion, supplementing T in buck semen extenders benefits spermatological parameters; particularly, 0.25 and 0.5 mM T could be used in Tris semen extenders during the cryopreservation process.Öğe INVESTIGATION OF THE IMMUNOCASTRATION EFFICACY AND LONGEVITY OF A SINGLE DOSE GNRH VACCINE IN YOUNG MALE RATS(Parlar Scientific Publications (P S P), 2019) Eski, Funda; Mis, Leyla; Tasal, Ibrahim; Uslu, Baris Atalay; Comba, BahatConventionally, immunization protocol require an initial priming immunization followed by an antigen boost for adequate immunity and long-term immunization. In this study we were evaluate the immunocastration efficacy and longevity of immunization with a single-dose GnRH vaccine (Repro-Bloc (TM)) in young male rats. Rats in the vaccine group (VAC, n: 35) were treated with GnRH vaccine as single-dose (200 mu l) and the control group (C, n: 15) were also injected with saline solution. Blood samples from all rats were taken monthly for 6 months and at 12th month. GnRH antibody titers, testosterone and leptin levels were determined by commercial RIA kits. Rats at 6th and 12th months were sacrificed for histologic analysis of testes. GnRH antibody levels were found similar in the VAC and C groups (P>0.05) at -1 week. As compared to the C, GnRH antibody levels (pg/ml) of the VAC group at 1st, 2nd, 3rd and 4th months rose continuously and reached the peak level at 5th month, decreased at 6th month and increased again by the 12th month (P<0.05), whereas in the C group GnRH antibodies weren't different (P>0.05). Compared to the unchanged testosterone levels within the process in C group, serum testosterone levels of the VAC group were lower at 2nd month and higher at 3th month, and thereafter lower (P<0.05). Leptin levels were no difference between groups (P>0.05). There were significant differences in the testicle weights, sperm motility, sperm numbers and abnormal sperm rates between VAC and C groups at 6th and 12th months (P< 0.05). Histological examination showed no significant difference in testes between groups at 6th and 12th months (P>0.05). In conclusion, although the vaccine reduced the germinative and endocrine testicular function from 6th months to 12th months with the single-dose GnRH vaccine, the single-dose vaccination did not provide adequate effect in the genital organs in male rats. Further studies are needed to determine an optimal single-dose, longevity of the vaccine and route of administration for complete suppression on the genital organs in different animal species.Öğe Protective effects of silymarin on methotrexate-induced damages in rat testes(UNIV SAO PAULO, CONJUNTO QUIMICAS, 2018) Yaman, Turan; Uyar, Ahmet; Kaya, Mehmet Salih; Keles, Omer Faruk; Uslu, Baris Atalay; Yener, ZabitThe present study aimed to investigate the protective effects of silymarin (SMN), an antioxidant, on methotrexate (MTX)-induced damage in rat testes. Thirty-two Wistar albino rats were divided into four groups (n = 8): control, MTX (20 mg/kg, i.p. on days 1 and 5), SMN (200 mg/kg, orally), and MTX + SMN (20 mg/kg, i.p. on days 1 and 5 and SMN 200 mg/kg orally) groups. At the end of the 6-week trial period, histopathological, immunohistochemical, biochemical, and spermatological analyses were performed on testes tissues. Histopathologically, MTX-induced damage, including depletion of germ cell and loos of spermatozoa, was significantly improved with SMN treatment. Immunohistochemically, the immunoreactivity of glutathione peroxidase 1 (GPx1) and manganese superoxide dismutase 2 (SOD2) were detected more intensely in the MTX + SMN group than in the MTX group. Biochemical examinations revealed that SMN supplementation decreased the lipid peroxidation and increased enzymatic antioxidants in the SMN-treated rats. Spermatologically, significant differences were found in the density, motility, dead-to-live sperm ratio, and abnormal sperm rate in the MTX + SMN group compared to the MTX group. In conclusion, SMN seems to have protective effects as an antioxidant against MTX-induced damage in rat testes.Öğe A retrospective examination of reproduction and survival rates of Van cats(AGRICULTURAL RESEARCH COMMUNICATION CENTRE, 2017) Cak, Bahattin; Uslu, Baris Atalay; Demirel, Ahmet Fatih; Gulyuz, Fetih; Yilmaz, Orhan; Kosal, VolkanVan cats are animals that often become the centre of attention due to their white hair, colourful eyes, and affectionate attitudes. However, it is nearly impossible to find research studies on the reproductive behaviours of Van cats. We conducted this study with the purpose of revealing the breeding efficiency properties and survival rates of Van cats. The study materials consisted of Van cats in the Van Cat Home of Yuzuncu Yil University's Van Cats Research Center. We determined the breeding efficiency properties using records obtained from 56 female cats over the course of four years. As a result of the study, we determined that the durations of oestrus, pregnancy, and lactation in Van cats were, respectively, 2.41 +/- 0.09, 63.75 +/- 0.27, and 98.45 +/- 1.18 days. We also determined the average number of kittens per delivery and the mating per pregnancy as, respectively, 3.30 +/- 0.19 and 13.71 +/- 0.74. In this study, we recorded the presence of 56 delivering cats, with a total of 185 kittens, 22 of which were dead (11.89%), and an 88.11% survival rate (%). The most common delivery type was twin deliveries (30%), and the highest rate of stillbirths was 4.86% in quadruplet deliveries.Öğe The effect of GnRH on the pregnancy ratio in low-yielding local race cows: comparison of different injection times(Springer, 2020) Uslu, Baris Atalay; Kocyigit, Alper; Sendag, Sait; Gulyuz, Fetih; Wehrend, AxelThe objective of this article was to investigate the efficiency of GnRH administrations at different time points after induced luteolysis on pregnancy rates in low-yielding subfertile cows. One thousand six hundred and ten healthy and subfertile dairy cows of different ages and races were used in this study. Cows were randomly divided into 4 groups. Estrus cycles were synchronized by two, with 11-day intervals, injections of the prostaglandin F2 alpha-analogue (PG). The artificial inseminations (AIs) of all animals were achieved at the 72nd and 96th hours following the last PG injection. The animals in groups I (n 257), II (n 337), and III (n 675) were used for the administration of a single dose of GnRH at different time points. Accordingly, GnRH was applied at 48th, 64th, and 72nd hours following the last PG injection in groups I, II, and III, respectively. Group IV was accepted as a control without GnRH injection (n 341). The pregnancy rates in groups I, II, III, and IV after transrectal pregnancy examinations were found to be 89.88%, 91.09%, 83.25%, and 77.12%, respectively. In our study, maximal pregnancy rates could be obtained with GnRH injections performed at 48th and 64th hours following luteolysis induction (P < 0.001). There was a 6-8% decrease in pregnancy rates due to the injection of GnRH in the 72nd hour (P < 0.001). These dramatic losses and gains in pregnancy rates in our study emphasized the necessity of taking the time of injection into account when using GnRH to stimulate ovulation. It can be said that the success of GnRH stimulation of ovulation is directly related to the follicle wave dynamics at the time of injection point and the character of a dominant follicle.Öğe The effects of levamisole and albendazole on spermatological parameters, testosterone levels, and sperm DNA damage in Saanen bucks(Springer, 2021) Yucel, Ufuk Mercan; Kosal, Volkan; Taspinar, Filiz; Uslu, Baris AtalayThis study aimed to investigate the effect of levamisole and albendazole on spermatological parameters, testosterone levels, and sperm DNA damage in Saanen bucks. For this purpose, twenty-four Saanen bucks were divided into three groups as control, levamisole, and albendazole administration group. The control group received only water (20 ml, oral), the levamisole group received 7.5 mg/kg of levamisole (2 oral tablets once daily for 2 days) + water (20 ml, oral), and the albendazole group received 7.5 mg/kg of albendazole (1 oral tablet) + water (20 ml, oral). Semen and blood samples were collected from all animals, both before drug application (day 0) and within a 2-day interval after drug application between day 1 (day of the treatment) and day 11. Spermatological parameters were evaluated immediately after collection. Testosterone levels were also measured from the blood samples with ELISA. Sperm DNA damage was determined with comet assay. The present research showed that especially albendazole administration decreased spermatological parameters and levamisole administration decreased testosterone levels. Significant sperm DNA damage was seen after both albendazole and levamisole administration. As a result, albendazole and levamisole administration should be used carefully on Saanen bucks, especially during the breeding season.
