Yazar "Yuksel, Murat" seçeneğine göre listele
Listeleniyor 1 - 11 / 11
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A different approach to diagnosis of subclinical mastitis: milk arginase activity(Univ Zagreb Vet Faculty, 2013) Kandemir, Fatih M.; Yuksel, Murat; Ozdemir, Necmi; Deveci, HuseyinThe aim of this study was to determine the relationships between subclinical mastitis and milk arginase activity in dairy cows. Thirty, various breed 4-8 year old cows from Firat University Animal Hospital were used in the study. Subclinical mastitis was diagnosed by the California Mastitis Test (CMT) combined with bacteriological examination of milk samples. The subclinical mastitis group consisted of fifteen clinically healthy but CMT and microbiologically positive animals. CMT and microbiologically negative animals (n = 15) served as the control group. Arginase activity in milk samples was measured by spectrophotometer using the thiocemicarbazide diacetylmonoxime urea (TDMU) method and protein was determined with the folin phenol reagent. Arginase activity in the milk of animals with subclinical mastitis (0.26 +/- 0.07 U/mg protein) significantly increased compared to the arginase activity in the milk from healthy animals in the control group (0.10 +/- 0.03 U/mg protein). Milk enzyme activity in the mildly severe disease (0.26 +/- 0.01 U/mg protein) was significantly lower than in the moderately severe disease (0.30 +/- 0.01 U/mg). No relationship was found between the nature of the bacterial infection of the cows with subclinical mastitis and their milk arginase activity. It was concluded that assay of milk arginase activity may be used as an additional laboratory method for the diagnosis of subclinical mastitis.Öğe Determination of 8-hydroxy-2?deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour(Ankara Univ Press, 2020) Ercan, Nazli; Yuksel, Murat; Kockaya, MustafaCanine transmissible venereal tumour (TVT) is a contagious cancer and sexually transmitted one dog to another by allogenic transfer of living cancer cells. The aim of this study is to examine the relationship between antioxidative metabolism and venereal tumour at Kangal Dogs and to show the size of oxidative damage it causes through 8-hydroxy-2'deoxyguanosine (8-OHdG). The materials of this study included the blood sera of 15 Kangal Dogs with TVT, and as a control group of 15 healthy Kangal Dogs. The sera levels of 8-OHdG were determined by competitive enzyme linked immunosorbent assay (ELISA) method. The level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) which are antioxidative enzymes were investigated by spectrophotometric methods as described. Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P<0.001). The levels of GPx were statistically different (P<0.05). In the light of the findings, which are obtained in this study, the transmissible venereal tumour, which is an infectious and treatable type of cancer, can lead to new studies that may be caused by oxidant-antioxidant metabolism.Öğe Determination of reproductive response to different synchronization methods and profitability during the transition to anoestrus in multiparous Kangal sheep(Veterinarni A Farmaceuticka Univerzita Brno, 2024) Takci, Abdurrahman; Kivrak, Mehmet Bugra; Murat, Hakan; Yuksel, MuratThe aim of the study was to determine a reproductively and economically successful synchronization method for Kangal sheep during the transition period from the breeding season to anoestrus. A total of 212 Kangal sheep were divided into three groups. On day 0, a sponge containing progesterone (P4) hormone was inserted intravaginally into the animals of Group 1 (n = 75). The sponges were removed 9 days later, and equine chorionic gonadotropin (eCG) and prostaglandin F2 alpha (PGF2 alpha) hormones were injected. Animals in Group 2 (n = 65) were given PGF2 alpha hormone at 9-day intervals. In addition to the second PGF2 alpha injection, eCG was also administered. A double dose of PGF2 alpha was administered to animals in Group 3 (n = 72) at 9-day intervals. The animals in this group were administered eCG along with the first PGF2 alpha injection. As a result of the applications, Group 1 showed a higher rate of oestrous than the other two groups (P < 0.05). There was no difference in pregnancy rates between Group 1 and the other groups. The pregnancy rate of Group 2 was significantly lower than that of Group 3. While there was no difference between the groups regarding multiple pregnancies, the fertility rate of Group 3 was higher than in the other two groups. Cost evaluations showed that the synchronization technique used in Group 3 was the most cost-effective. It was determined that the synchronization method used in Group 3 is preferred for Kangal sheep during the transition period.Öğe The effect of dystocia on passive immune status, oxidative stress, venous blood gas and acid-base balance in lambs(ELSEVIER SCIENCE BV, 2018) Aydogdu, Ugur; Coskun, Alparsian; Yuksel, Murat; Basbug, Onur; Agaoglu, Zahid TevfikThe aim of this study was to research the effect of dystocia in lambs on passive immune status, oxidative stress, venous blood gas and acid-base balance. The study involved 20 lambs of both sexes. The lambs were divided into two groups, normal (n = 10) and dystocia (n = 10) births. Blood samples for blood gas, MDA, GSH, GSH-Px and IgG were collected at 0 h and at periodic intervals of 1, 3, 6, 24 and 48 h. Both groups' 0 h pH and glucose levels were under normal values and their pCO(2) and lactate levels were above normal values. The blood pH increased in both groups in the 1st, 3rd, 6th, 24th and 48th hours at a significant rate when compared to 0 h and the pCO(2) level decreased at a significant rate. Moreover, the blood pH of the dystotic lambs was found to be low according to the normal birth group in 0 h and the 1st, 3rd, 6th and 48th hours, but no statistical difference was determined. When it was compared to the normal birth group, the pO(2) and O-2 saturation in dystotic lambs was low at all hours but a statistical difference was determined only in the 48th hour. Lactate levels decreased in both groups. When the oxidative stress parameters were analysed, no statistical difference was determined although the malondialdehyde (MDA) level of the dystotic lambs was found to be higher than the normal birth group and its glutation (GSH) and glutation peroxidase (GSH-Px) levels were lower. When the IgG levels of the lambs at the postnatal 48th hour were examined, no difference was found between the two groups. In conclusion, it was deduced that dystocia caused negative effects on the oxidative stress and blood gases but the changes in the lambs' passive immunity, oxidative stress, blood gases and vital functions could be reduced to a minimum level with early intervention in dystocia.Öğe The effect of oxytocin and cloprostenol application via umbilical artery immediately after dystocia on time and rate of fetal membrane removal in cows(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2012) Akar, Yasar; Kizil, Omer; Saat, Nevzat; Yuksel, MuratThe aim of this clinical study was to investigate the effects of oxytocin and cloprostenol application via umbilical artery on the time and rate of fetal membrane removal in cows with dystocia immediately aft er parturition. Metabolic profiles indicated certain serum biochemical parameters that were also determined in the cows. The study was performed on 60 cows with dystocia. All of the cows were randomly divided into 3 equal subgroups. The first group was injected with 100 IU oxytocin, while the second group was treated with 0.15 mg cloprostenol and the third group (the control group) received 10 mL 0.9% NaCl via intraumbilical artery. Blood samples were randomly taken from the jugular veins of 10 cows from each group. No significant differences were determined between the groups with regard to the values of aspartate aminotransferase, alkaline phosphatase, creatine kinase, total protein and bilirubin, albumin, glucose, Ca, Na, or K (P > 0.05). The lactate dehydrogenase level in the oxytocin group was determined to be significantly lower when compared to the cloprostenol and control groups (P < 0.05). The value of gamma-glutamyl transferase in the oxytocin group was significantly higher than in the other groups (P < 0.05). The time and rate of fetal membrane removal were not different among the groups (P > 0.05). In conclusion, the administration of combinations of other uterotonics and enzymes together with these agents should be investigated to determine the effects on cows with retained placenta prophylaxis. Future applications may also study the effects of these agents on fertility and time and rate of fetal membrane removal in cows with normal parturition and dystocia at the herd level.Öğe Effects of gentamicin on spontaneous and agonist-induced in vitro contractions of isolated myometrial tissue from pregnant cows(Polish Soc Veterinary Sciences Editorial Office, 2020) Yuksel, Murat; Ocal, Halts; Ayar, AhmetThe aim of the present study is to examine the effects of gentamicin sulphate on spontaneous, oxytocin and PGF(2 alpha) induced in vitro contractions of myometrium isolated from pregnant cows. Myometrial strips were obtained from healthy pregnant cows and suspended in a covered organ bath filled with Krebs' solution at 37 degrees C (pH 7.4) continuously bubbled with 95% oxygen and 5% carbon dioxide: isometric contractions were recorded using an isometric force displacement transducer. After the stabilization of spontaneous contractile activity during a 90-minute equilibration period, contractions were recorded for 20 minutes (control). Gentamicin sulphate was then added to the tissue bath cumulatively and the responses were recorded every 20-minutes for each consecutive dose of gentamicin. In agonist-induced contractions, oxytocin or PGF(2 alpha) was added to the tissue bath at the end of the equilibration period and the same protocol was followed to investigate the effects gentamicin on these agonist-induced contractions. Gentamicin decreased the frequency and inhibited the amplitude of the spontaneous contractions in a dose dependent manner (p < 0.05). The mean frequency and amplitude of oxytocin-induced contractions was significantly inhibited by the application of gentamicin (p < 0.05). Gentamicin also inhibited the contractions induced by PGF(2 alpha) in a dose dependent manner (p < 0.05). This study showed gentamicin inhibited, depending on the dosage, oxytocin and PGF(2 alpha) induced contractions of myometrium isolated from pregnant cows. Upon clinically examining the findings obtained by the study, gentamicin can be used as an antibacterial in septic abort and chorioamniotis in order to prevent premature birth, abortion and early uterus contractions. Further studies are necessary to test whether the same effect will take place in vivo and to examine the effects of long-term use of gentamicin on offsprings.Öğe Evaluation of Serum Amyloid A and Procalcitonin in Some Inflammatory Diseases of Cattle(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020) Basbug, Onur; Yurdakul, Ibrahim; Yuksel, MuratRecent study in humans and animals has been focused on inflammatory biomarkers that infectious diseases, such as serum amyloid A (SAA), procalcitonin (PCT), that may more accurately and efficiently diagnose inflammation. The aim of this study was to evaluate SAA and PCT levels in the diagnosis of cattle with inflammatory disease. Ten healthy control cattle and 64 patients with systemic inflammatory response syndrome (SIRS) were included in cattle. Inflammatory disease in cattle was diagnosed based on clinical signs and the laboratory examination in clinically suspected cases. SAA and PCT concentrations were measured with a commercial ELISA assay for cattle. SAA and PCT concentrations in cattle with inflammatory disease were significantly higher than in the healthy controls (respectively, P<0.001, P<0.008). Concentrations of SAA and PCT at admission were significantly (r = 0.376, P<0.01) correlated with outcome in cattle with inflammatory conditions. The cut-off value of SAA and PCT for healthy and inflammatory cattle was determined 28.52 mu g/mL and 149.55 pg/mL. In conclusion, PCT levels may be used as an alternative to serum SAA measurement as an indicator of acute inflammation in cattle. Serum PCT concentrations were similar to 9 times higher in the cattle with peritonitis than in the healthy cattle, suggesting that PCT could be a useful marker of peritonitis in cattle.Öğe Neutrophil to Lymphocyte Ratio is Predictor of Atrial Fibrillation Recurrence After Cardioversion With Amiodarone(SAGE PUBLICATIONS INC, 2015) Karavelioglu, Yusuf; Karapinar, Hekim; Yuksel, Murat; Memic, Kadriye; Sarak, Taner; Kurt, Recep; Yilmaz, AhmetBackground: In this study, our aim is to examine the role of the neutrophil to lymphocyte ratio (NLR) in the predictions of recurrence under long-term follow-up in patients whose sinus rhythms (SRs) were restored with amiodarone in acute atrial fibrillation (AF). Methods: Retrospectively, patients with acute AF, which successfully converted to the SR with amiodarone treatment, were recruited into the study. Patients experiencing the first AF attack were enrolled to the study and followed up for 5 years (median 23 months, 25-75 percentiles 12-24 months). Neutrophil to lymphocyte ratio was computed as absolute neutrophil count divided by lymphocyte count. Results: A total of 218 patients were recruited into the study and followed up for 21.6 13.9 months; 87 (40%) patients had 1 recurrent AF attack within this period. The follow-up of 131 (60%) patients resulted in persisted SR without any other AF attack. Groups were similar in terms of age and gender. Left atrium (LA) diameter and NLR were increased, and platelet count and lymphocyte count were decreased in patients with AF recurrence in univariate analysis (P < .05 for all). Only LA diameter (for per mm, 1.077 [1.021-1.136], P = .006) and NLR (1.584 [1.197-2.095], P = .001) were independent predictors of AF recurrence in the multivariate analysis. Conclusion: Increased NLR is a marker of increased inflammation and may serve as simple, cheap, and readily available predictors of recurrence in the long-term follow-up of patients admitted with acute AF and successfully converted to SR with amiodarone.Öğe Pharmacokinetics of letrozole and effects of its increasing doses on gonadotropins in ewes during the breeding season(Wiley, 2024) Kivrak, Mehmet Bugra; Corum, Orhan; Yuksel, Murat; Turk, Erdinc; Corum, Duygu Durna; Tekeli, Ibrahim Ozan; Uney, KamilLetrozole is a non-steroidal, third-generation aromatase inhibitor used in humans. Although letrozole is not approved for use in animals, it is used off-label in cases of synchronization and infertility. The aim of this study was to determine the pharmacokinetics of letrozole after a single intravenous administration at three different doses in ewes during the breeding season and its effect on gonadotropins (luteinizing hormone (LH) and follicle-stimulating hormone (FSH)) at the beginning of proestrus. The study was carried out on 24 healthy Merino ewes. Ewes were randomly divided into four groups (n = 6) as control, 0.5, 1, and 2 mg/kg. Plasma concentrations of letrozole were measured using HPLC-UV and were analyzed by non-compartmental analysis. LH and FSH concentrations were measured with a commercial ELISA kit. The terminal elimination half-life (t(1/2 lambda z)) was significantly prolonged from 11.82 to 18.44 h in parallel with the dose increase. The dose-normalized area under the concentration-time curve (AUC) increased, and total body clearance (Cl-T) decreased at the 1 and 2 mg/kg doses (0.05 L/h/kg) compared with the 0.5 mg/kg dose (0.08 L/h/kg). There were no differences in the volume of distribution at steady-state and initial (C-0.083h) plasma concentration values between dose groups. The decreased ClT, prolonged t(1/2 lambda z), and increased AUC at increasing doses showed the nonlinear kinetic behavior of letrozole. Letrozole significantly reduced LH concentration without affecting FSH concentration at all doses. As a result, letrozole has the potential to be used in synchronization methods and manipulation of the follicular waves due to its effect on LH secretion.Öğe The effects of Caesarean section on lipid peroxidation and some antioxidants in the blood of newborn calves(Univ Zagreb Vet Faculty, 2013) Erisir, Mine; Kandemir, Fatih M.; Yuksel, MuratThe mode of delivery may have a considerable effect on the state and health of the newborn. Therefore, the purpose of this study was to investigate the effect of a Caesarean section on oxidant and antioxidant status in newborn calves. This study included the neonates of 8 normally calved cows and 8 cows who had undergone by Caesarean section. Malondialdehyde concentrations were significantly higher in the calves delivered by caesarean section compared to the calves of normally calved cows, but catalase activity was significantly lower (P<0.01). Glutathione concentrations tended to increase in the calves delivered by Caesarean section compared to the calves of normally calved cows. There were no significant differences in the glutathione peroxidase activity between the groups. Our results suggest that the malondialdehyde concentrations and catalase activities, which show lipid peroxidation and antioxidant status in newborn calves, change due to Caesarean section. In conclusion, newborn calves are exposed to higher oxidative stress in Caesarean section.Öğe The pharmacokinetics of sulpiride and its effect on sexual behaviours and LH concentrations in anestrous does (Capra hircus)(Elsevier, 2025) Yuksel, Murat; Corum, Orhan; Kivrak, Mehmet Bugra; Corum, Duygu Durna; Turk, Erdinc; Takci, Abdurrahman; Yardimci, Sara BusraThe aim of this study was to investigate the pharmacokinetics of sulpiride and its effect on sexual behaviours and LH concentrations in anestrous does (Capra hircus). This study was carried out in two stages: pharmacokinetics (stage I) and effect on LH pulsatility, concentration, and estrus display (stage II). In the stage I, sulpiride was administered via intravenous (IV), intramuscular (IM), subcutaneous (SC) and oral routes to does at a dose of 0.6 mg/kg. In the stage II, sulpiride was administered intramuscularly at a dose of 0.6 mg/kg every 12 hours for 10 days. Plasma concentrations of sulpiride were measured using HPLC and pharmacokinetic parameters were calculated by non-compartmental analysis. LH concentrations were quantified using ELISA. The terminal elimination half-life, apparent volume of distribution, and total body clearance of sulpiride following IV administration were 1.76 h, 0.38 L/kg, and 0.15 L/h/kg, respectively. The peak plasma concentration of IM and SC administration was 1.39 and 0.83 mu g/mL at 0.53 and 0.78 h, respectively. The bioavailability of sulpiride was 103.30 % for the IM route and 72.21 % for the SC route. Sulpiride showed erratic and low absorption after oral administration. While LH concentrations decreased significantly after sulpiride administration, the LH plus frequency increased significantly. In conclusion, sulpiride with distinctive effect on LH pulse frequency has the potential to be used in protocols for hastening cyclicity. However, more studies are needed on the use of sulpiride in estrus induction protocols.
