Yazar "Zontul, Cemile" seçeneğine göre listele
Listeleniyor 1 - 11 / 11
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe ASIC3 gen polimorfizmi ile fibromiyalji sendromu arasındaki ilişkinin incelenmesi(Cumhuriyet Üniversitesi, 2017) Zontul, Cemile; Siliğ, YavuzFibromiyalji sendromu (FMS), uzun süreli yaygın vücut ağrısı ve tanımlanmış kronik bir ağrı sendromudur. ASIC3 iyon kanalları kas ağrı reseptörlerini etkinleştirmekte ve kronik kas ağrılarına neden olmaktadır. Bu çalışmada Türk popülasyonunda, ASIC3 polimorfizmi ile FMS arasındaki ilişki incelendi. ASIC3 genindeki polimorfizim (rs4148855 ve rs2288646) 175 hasta ve 176 sağlıklı kontrolde Real-Time PCR metoduyla belirlendi. Elde edilen sonuçlar, lojistik regresyon ve Khi-kare (?2) testi kullanılarak değerlendirildi. FMS'li hastalar ve kontroller uyku bozukluğu, yorgunluk, baş ağrısı, sabah yorgunluğu, göz kuruluğu, ağız kuruluğu, bacak uyuşması, konsantrasyon güçlüğü, yumuşak dokularda şişkinlik hissi ve ailede fibromiyalji öyküsü bulunması açısından lojistik regresyon analizi ile değerlendirildiğinde, istatistiksel olarak anlamlı bir ilişki bulundu (p<0,05). Fibromiyalji hastalarında, ASIC3 polimorfizmi (rs4148855 ve rs2288646) ile bu semptomlar istatistiksel olarak ?2 yöntemiyle değerlendirildiğinde genotipler arasında anlamlı bir farklılık tespit edilmedi (p>0,05). İncelenen Türk popülasyonunda ASIC3 rs4148855 polimorfizmi ile Fibromiyalji sendromu arasında anlamlı bir ilişki bulunamadı (p>0,05). Benzer olarak ASIC3 rs2288646 polimorfizmi ve Fibromiyalji sendromu arasında anlamlı bir ilişki tespit edilmedi (p>0,05).Öğe ASSOCIATION OF SERUM ANNEXIN A3 (ANXA3) LEVELS WITH AGE-RELATED MACULAR DEGENERATION Yaşa Bağlı Makula Dejenerasyonu İle Serum Anneksin A3 (Anxa3) Düzeylerinin İlişkisi(Yozgat Bozok Üniversitesi, 2023) Öztürk, Alpaslan; Gül, Mehmet Ali; Ağbektaş, Tuğba; Zontul, Cemile; Çiftçi, Cem Hakan; Taş, Ayça; Yeter, Duygu YalınbaşABSTRACT Objective: Age-related macular degeneration (AMD) is the most common cause of advanced visual loss in developed societies. ANXA3 contributes to tumor development by affecting cell signaling pathways, cell profiling, invasion, metastasis and cell apoptosis. The aim of this study was to compare serum ANXA3 levels in wet type and dry type macular degeneration patients with the control group and to investigate whether there is a significant difference between the groups. Material and Methods: A total of 78 patients (39 patients with wet AMD, 39 patients with dry AMD diagnosed at the same time period and 39 healthy controls without any previous diagnosis of macular degeneration) were enrolled in this study. ANXA3 levels were determined by enzyme-linked immunosorbent assay (ELISA). Results: Serum ANXA3 levels were found to be significantly higher in wet AMD than in the control group (p=0.005). In addition, the difference between serum ANXA3 levels of wet and dry AMD patients was found to be significant. It was observed that there was a significant increase in wet type AMD patients (p=0.004). However, no significant difference was found in ANXA3 levels in dry type AMD compared to the control group (p=0.444). Conclusion: These findings suggest that ANXA3 protein may play an important role in age-related macular degeneration.Öğe CLASSIFICATION OF FIBROMYALGIA SYNDROME WITH SUPPORT VECTOR MACHINES(İstanbul Üniversitesi, 2017) Zontul, Cemile; Hayta, Emrullah; Zontul, Metin; Taş, Ayça; Siliğ, YavuzFibromyalgia syndrome (FMS) is a long-term common body pain and a defined chronic pain syndrome. Fibromyalgia syndrome is a difficult disease to diagnose. For this reason, after many unnecessary treatments are applied to the patient, the diagnosis of FMS is usually delayed by clinical examination and evaluation of the patient's complaints. In this respect, there is a need for a decision support system that will facilitate the diagnosis of FMS. In this study, by using the questions asked 351 respondents, 175 FSM patients and 176 healthy control subjects and experimental data, FMS classification was performed with Support Vector Machines which is one of machine learning methods and 85% success was achieved.Öğe Determination of the impacts of titanium dioxide nanoparticles on a number of xenobiotic-metabolizing enzymes in rat liver(Burdur Mehmet Akif Ersoy University, 2020) Taş, Ayça; Keklikcioğlu Çakmak, Neşe; Agbektaş, Tuğba; Zontul, Cemile; Özmen, Esma; Siliğ, YavuzNanotechnology techniques are used in many applications, such as cancer treatment, radiological imaging methods, and pharmaceutical industry, as well as in the microbiology field, tissue regeneration, injury healing, treatment of some chronic diseases, and production of vaccines. Whereas products of nanotechnology have a lot of benefits mentioned in our life, they also have some systemic, genetic, and toxic effects in organisms. This study’s goal was to reveal the impacts of titanium dioxide (TiO2) nanoparticles on a number of xenobiotic-metabolizing enzymes in the rat liver fraction. In the current research, adult Wistar albino rats having a weight of approximately 150-200 g and fed under normal conditions were utilized. The incubation of four various concentrations of TiO2 nanoparticles (0.5, 1, 5, and 10 ppm) was performed in the liver fractions. We studied the effects of TiO2 nanoparticles on some enzymes identified in the microsomal fraction, such as N-nitrosodimethylamine demethylase (cytochrome P4502E1), NADPH cytochrome c reductase, NADH cytochrome b5 reductase, and other enzymes found in the cytosolic fraction, e.g. glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PDH), and glutathione level (GSH). GST, G6PDH, NADH-cytochrome b5 reductase, and NADPH cytochrome c reductase levels decreased statistically significantly, whereas the GSH level increased significantly in comparison with controls (p 0.05). Accordingly, in this study, we have shown that TiO2 nanoparticles are capable of inhibiting xenobiotic-metabolizing enzymes. Therefore, this inhibition can affect the detoxification system negatively.Öğe Diagnostic Significance of Chemerin and Chitinase 3-Like Protein 1 Levels in Cholelithiasis(2023) Öztürk, Alpaslan; Gül, Mehmet Ali; Zontul, Cemile; Güçlü, Gülşen Boztepe; Ağbektaş, Tuğba; Taş, Ayça; Topçu, ÖmerObjectives: Cholelithiasis is a common gallbladder disease that refers to the formation of gallstones. In this study, we investigated the relationship between Cholelithiasis and Chitinase 3-like-1 protein (Chi3L1) and Chemerin levels. Materials and Methods: Our study was conducted in 88 individuals, 44 of whom were healthy and 44 of whom had cholelithiasis. Chemerin and Chi3L1 serum levels were measured by enzyme-linked immunsorbent assay-ELISA method. The results were then analyzed using SPSS. Results: When cholelithiasis patient groups were compared with healthy groups, a significant increase was found in Chemerin levels in cholelithiasis patients (6.28-4.68, p<0.001). Chi3L1 concentration values were not statistically different between groups (p=0.460). After receiver operating characteristic analysis, the Chemerin result was statistically significant in predicting disease [p<0.001, area under the curve: 0.795 (0.697-0.893)], Chi3L1 was statistically insignificant in predicting disease. While the difference between the patient and control groups in terms of creatinine, albumin, amylase, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, direct bilirubin, sodium, and calcium was statistically significant (p<0.05), the difference between Chi3L1 concentration and creatinine and total protein was statistically significant (p<0.05). Conclusion: Our study suggests that chemerin may be a new biomarker in the diagnosis of cholelithiasis. In addition, since our study has not been done before, it can bring a new perspective to the literature.Öğe High levels of cathepsin S and cystatin C in patients with fibromyalgia syndrome(Wiley, 2020) Kocak, Ibrahim; Hizmetli, Sami; Tas, Ayca; Karadag, Ahmet; Zontul, Cemile; Silig, YavuzObjectives Although the etiopathogenesis of fibromyalgia syndrome (FM) is not yet clear, central sensitization is thought to be responsible for the pathogenesis of FM. The aim of this study was to compare the serum cathepsin S (CatS) and cystatin C (CysC) levels between patients with FM and healthy control subjects. Methods This study was conducted in the Physical Medicine and Rehabilitation Clinic between January 2019 and October 2019. The study included 145 FM patients newly diagnosed with primary FM according to the 2010 American College of Rheumatology criteria and 129 healthy volunteers. The age, gender, and body mass index (BMI) of the participants were recorded. Venous blood samples were collected from both groups for the measurement of the levels of serum CatS and CysC. The functional status of FM patients was evaluated using the Fibromyalgia Impact Questionnaire (FIQ). Results No statistically significant difference was determined between the patient and control groups in terms of age, gender, and BMI (P > .05). A comparison of the serum CatS and CysC levels of the FM and control groups revealed a statistically significant difference (P = .001). No correlation was determined between FIQ and serum CatS and CysC levels (P > .05). Conclusion Serum CatS and CysC levels were found to be higher in FM patients. However, there was no correlation between the functional status of FM patients and serum CatS and CysC levels. These results can be of guidance for further clinical studies of the etiopathogenesis and treatment of FM.Öğe In vitro cytotoxicity, gene expression, bioinformatics, biochemical analysis, and in silico analysis of synthesized carbonitrile derivatives(Springer Wien, 2025) Tuzun, Burak; Agbektas, Tugba; Naghiyev, Farid N.; Tas, Ayca; Zontul, Cemile; Ozum, Unal; Khalilov, Ali N.In this work, an efficient single-step green synthesis protocol of 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles was applied with the purpose of their biological activity studies. It was found that the cytotoxic activities of 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles in the SHSY-5Y cancer cell line were most active after 72 h of incubation. It was determined that 1,6-diamino-4a-methyl-3-oxo-2,8-diphenyl-2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitrile showed higher anticancer activity on SHSY-5Y cancer cells compared to other molecules. The expression levels of Myc-N, Casp2, Tp53, RAD51, BRCA2, MDM2, BAX and NF-kappa B1 genes were analyzed by RT-PCR method by applying 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles to the SHSY-5Y neuroblastoma cancer cell line. It was observed that there were differences in expression levels between study groups and all differences were found to be statistically significant (p < 0.000). Bioinformatics analysis was performed using the STRING v 11 Protein interaction tool. When the molecules in the SHSY-5Y cell line were compared to the control group, an 80% increase in GSH levels was observed in 1,6-diamino-4a-methyl-3-oxo-2,8-diphenyl-2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitrile and 1,6-diamino-8-(4-chlorophenyl)-4a-methyl-3-oxo-2-phenyl-2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitrile groups. SOD enzyme activity increased significantly in the former group compared to the control and other groups. LDH activity was detected at a higher rate in this cell line compared to the control group. Calculations were made for 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles using the Gaussian software package on B3LYP, HF, and M06-2 x levels with the 6-31++g(d,p) basis sets. The activities of the 2,3,4,4a-tetrahydroisoquinoline-5,7-dicarbonitriles found in the study against SH-SY5Y protein (2F37, 3PBL and 5WIV), comparison has been made. MM/GBSA methods are calculated binding free energy for all molecule and 3PBL protein. Afterwards, ADME/T analysis was performed to examine the effects of the molecules on human metabolism.Öğe Investigation of The Effect of B-108 Contaınıng Azomethin Group On DNA Repair Gene(Sivas Cumhuriyet University, 2022) Eğilmez, Elif; Zontul, Cemile; Huseynzada, Alakbar; Aliyeva, Gunel; Hasanova, Ulviyya; Taş, Ayça; Siliğ, YavuzObjective: Cancer arises as a result of the failure of the mechanisms controlling normal division in a group of cells. It is known that some new synthesis compounds intended for use in cancer treatment have anti-fungal, anti-bacterial, anti-carcinogenic effects. In this study, it was aimed to apply the newly synthesized B-108 compound to the A-549 cell line and then to investigate the effect of this compound on the ERCC1 gene expression profile. Materials and Methods: Firstly, compound B-108 was synthesized in our study. Afterwards, this synthesized molecule was applied in eight different concentrations (1-100 μg/ml) in A-549 cell line and 3-(4,5-dimethylthiazol-2-yl)-2,5-yl for 24 hours, 48 hours and 72 hours. Anticancer activities were determined using diphenyltetrazolium bromide (MTT) method. Expression level of DNA repair gene (ERCC1) was determined using RT-PCR method. Results: As a result, it was determined that the molecule applied to the A-549 cell line showed the highest activity after 72 hours of incubation. It was observed that the ERCC1 gene expression of the molecule applied on lung cancer was lower than the control group. Discussion: Considering the current study results, low expression of ERCC1 shows that compound B-108 correlates with overall survival on lung cancer cells.Öğe PD-L1 geni susturulmuş mide kanseri hücrelerinde dosetaksel ilacının antikanser aktivitesi ile hücre döngüsü ve apoptozis genleri arasındaki ilişkinin araştırılması(Sivas Cumhuriyet Üniversitesi, 2023) Zontul, Cemile; Siliğ, YavuzBu çalışmada, PD-L1 geni susturulmuş AGS mide kanseri hücre hatlarında, dosetaksel ilacının sitotoksik aktivitesi ve MYC, CASP2, Tp53, RAD51, BRCA2, MDM2 ve BAX genlerinin ekspresyon düzeyleri, apoptoz üzerine etkisinin araştırılması amaçlanmıştır. Çalışmamızda öncelikle hücre döngüsü ve apoptozda görevli proteinlerin birbirleriyle ve PD-L1 geni ile etkileşimini belirlemek için String v11 programı kullanıldı. Analiz sonucunda PD-L1 proteininin MYC, CASP2, Tp53, RAD51, BRCA2, MDM2 ve BAX proteinleri ile etkileşimleri belirlendi. Mide kanseri teşhisi alan ve ameliyat olan hastalardan alınan doku örneklerinde PD-L1, MYC, CASP2, Tp53, RAD51, BRCA2, MDM2 ve BAX genlerinin ekspresyon düzeyleri tespit edildi. Dosetaksel ilacı AGS hücre hattına sekiz farklı konsantrasyonda (1-100 µg/mL) 24, 48 ve 72 saat süreyle uygulanmış ve antikanser aktiviteleri MTT yöntemi ile belirlenerek IC50 değeri hesaplandı ve hücrelere uygulandı, RNA izole edildi ve cDNA sentezlendi. siRNA havuzu ile PD-L1 geni susturuldu. RT-PCR cihazında ??CT yöntemi kullanılarak hücre döngüsü, apoptoz genleri ve PD-L1 geninin ekspresyon düzeyleri analiz edildi. PD-L1 geni susturulmuş ve dosetaksel uygulanmış hücrelerde apoptotik cisimcikleri belirlemek için DAPi boyama işlemi gerçekleştirildi. Mide kanseri tümör dokularında BRCA2, BAX, RAD51 genlerinin ekspresyonu kontrol grubuna kıyasla azaldığı; PD-L1, MDM2, MYC, CASP2, TP53 genlerinin ise kontrol grubuna kıyasla arttığı tespit edildi. PD-L1 siRNA transfekte edilmiş AGS hücre hattında PD-L1, MDM2, BRCA2, BAX, MYC, CASP2, Tp53 genlerinin ekspresyonunun kontrol grubuna kıyasla azaldığı, RAD51 geninin ise kontrol grubuna kıyasla arttığı tespit edilmiştir. Sonuç olarak mide kanseri tedavisinde PD-L1 genin susturulması ve DTX ile kombine tedavi umut vericidir. Ancak, konuyla ilgili daha kapsamlı in vitro ve in vivo çalışmalara ihtiyaç duyulmaktadır.Öğe Potassium Ion Channel Protein (KCNH) Levels in Patients with Fibromyalgia Syndrome(CMB Association Publiher, 2021) Taş, Ayça; Hayta, Emrullah; Karadağ, Ahmet; Zontul, Cemile; Özmen, Esma; Aydın, Süleyman; Siliğ, YavuzAlthough there is not yet full clarity of the pathogenesis of fibromyalgia syndrome (FM), central sensitization is considered to be responsible. The purpose of this study was to measure the plasma levels of potassium ion channel proteins (human KCNH2, KCNH6 and KCNH7) in FM patients and healthy control subjects. The study sample includes 76 newly diagnosed FM patients and 79 healthy individuals. Venous blood samples were taken to measure the plasma levels of KCNH2, KCNH6 and KCNH7. Pain severity in FM patients was assessed using a visual analog scale (VAS). Bioinformatics analysis was performed using the STRING v 11 Protein interaction tool. Age, gender and body mass index were seen to be similar in both groups. In comparisons between FM and control groups, KCNH2 plasma levels was found to be significantly lower in the FM group. No significant correlation was found between plasma levels of KCNH2, KCNH6 and KCNH7 protein levels and VAS score of patients with FM. The KCNH2 protein had a high homology score with 9 proteins. The plasma levels of KCNH2 FM patients were found to be lower than those of the healthy control subjects, no difference was determined in respect of the plasma levels of KCNH6 and KCNH7. These results may be of use in guiding future studies on the pathogenesis of FM.Öğe The relationship between ASIC3 gene polymorphism and fibromyalgia syndrome(Walter De Gruyter Gmbh, 2024) Zontul, Cemile; Tas, Ayca; Hayta, Emrullah; Silig, YavuzObjectives: Fibromyalgia syndrome (FMS) is a chronic pain syndrome characterized by widespread body pain over a long period, the cause of which is not yet clearly known. FMS patients usually have high pain sensitivity. We aimed to investigate whether rs4148855 and rs2288646 polymorphisms of acid-sensing ion channel 3 (ASIC3), one of the factors contributing to pain, cause a predisposition to FMS in the Turkish population.Methods: ASIC3 gene rs4148855 and rs2288646 polymorphisms in DNA samples obtained from blood samples of 175 patients with FMS and 176 healthy individuals were analyzed by real-time polymerase chain reaction (RT-PCR) using a hydrolysis probe. Statistical data were obtained by chi-square (chi(2)) test and logistic regression analysis.Results: No significant association was found between ASIC3 gene rs4148855 and rs2288646 polymorphisms and the Turkish population's FMS group and control group (p>0.05).Conclusions: As a result, no significant association was found between the genotype and allele distributions of ASIC3 polymorphism (rs4148855 and rs2288646) in patients with FMS compared to controls in the Turkish population. Further studies are needed to elucidate the relationship between ion channels and FMS to elucidate the mechanisms of FMS.
