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Öğe The Effects of Sunitinib in Healthy and Cisplatin-Induced Rats(Wiley-V C H Verlag Gmbh, 2023) Demirtas, Levent; Gurbuzel, Mehmet; Akbas, Emin Murat; Tahirler, Hilal; Karatas, Ozhan; Arslan, Yusuf KemalSunitinib is a multitargeted kinase inhibitor that inhibits many receptor tyrosine kinases and has been used in the treatment of gastrointestinal stromal tumors, metastatic renal cell carcinoma, and pancreatic neuroendocrine tumors. In this study, the effects of sunitinib given to rats, both alone and after stress with cisplatin, were investigated. The animals were divided into four groups - (1) control group (C) administered interperitoneally with a single dose 0.9 % saline, (2) Cis group administered a single dose (7 mg/kg) of cisplatin, (3) Sun group administered 10 mg/kg sunitinib for seven days, and (4) Cis+Sun group administered 10 mg/kg sunitinib for seven days after a single dose (7 mg/kg) of cisplatin. After these applications, the rats were sacrificed, and blood and tissue samples were taken for biochemical and histopathological evaluations. Sunitinib did not show any effect on urea, creatine, and kidney IL1 beta and TGF-beta 3 expression levels when administered alone; it increased ALT, AST, and IL-38 levels. When sunitinib was given to the cisplatin-induced rats, it was observed that the increase in ALT, AST, and IL-38 levels increased more than the rats that was given only sunitinib. According to the data obtained, sunitinib does not cause a significant change in kidney tissue under both normal and stress conditions, while it creates stress in liver tissue. In addition, its toxicity in the liver becomes more certain as a result of its combination with cisplatin.Öğe New benzimidazole derivatives containing hydrazone group as anticancer agents: Inhibition of carbonic anhydrase IX and molecular docking studies(Wiley-V C H Verlag Gmbh, 2025) Bostanci, Hayrani Eren; Yildiz, Mehmet Taha; Kapancik, Serkan; Inan, Zeynep Deniz Sahin; Kilic, Haci Ahmet; Guler, Ozen Ozensoy; Cevik, Ulviye AcarIn this study, we propose identifying potential novel compounds targeting carbonic anhydrase (CA) IX and anticancer activity. To study the impact of these synthesized compounds on CA IX and anticancer activity, we have developed and synthesized novel benzimidazole-hydrazone derivatives (3a-3j). The target compounds' H-1 NMR (nuclear magnetic resonance), C-13 NMR, and high resolution mass spectrometry spectra were used to confirm their chemical structures. L929 (healthy mouse fibroblast cell line) used as control healthy cell line and MCF-7 (breast cancer), C6 (rat glioblastoma), HT-29 (colon cancer), cells were used in cell culture studies. As a result of cell culture studies, it was determined that the newly synthesized compounds 3d and 3j had cytotoxic effects on colon cancer. Again, it was determined that the compound 3d had a more toxic effect than cisplatin on both breast cancer and glioma cells. According to the CA IX activity results, compounds 3d and 3j were found to have the highest activity. Compounds 3d and 3j are essential for having anti-cancer properties and inhibiting the carbonic anhydrase IX enzyme. Molecular docking of these compounds was carried out in the active site of CA IX. Flow cytometry and immunofluorescence microscope analyses also confirmed that these compounds had cytotoxic effects on cancer cells.Öğe Density Functional Theory Computations and Experimental Analyses to Highlight the Degradation of Reactive Black 5 Dye(Wiley-V C H Verlag Gmbh, 2023) Yildiz, Sayiter; Canbaz, Gamze Topal; Kaya, Savas; Maslov, Mikhail M.The oxidative degradation of Reactive Black 5 (RB5) in aqueous solution was investigated using Fenton (FP), photo Fenton (P-FP), sono Fenton (S-FP), and sono photo Fenton (S-P-FP) processes. Degradation experiments showed efficient dye degradation for FP, P-FP, S-FP, and S-P-FP under optimal conditions. The half-life values of the reaction calculated for first-order reaction kinetics showed that the S-FP process is faster than the FP and P-FP processes. Using DFT calculations, the chemical reactivities of the studied chemical systems were analyzed. Especially the calculated chemical hardness values reflect the reactivities of the dye and the dye-Fe2+ complex. The calculated binding energy between the Fe2+ ion and RB5 of 15.836 eV is compatible with the prediction made in the light of the principle of hard and soft acids and bases. The computed data supported the experimental observations.Öğe Benzothiazole Derived Ether Hybrids as Potent Anti-Thymidine Phosphorylase Agents: Synthesis, In Vitro, and Computational Investigations(Wiley-V C H Verlag Gmbh, 2025) Usman, Muhammad; Alam, Aftab; Zainab; Khan, Majid; Tuezuen, Burak; Ayaz, Muhammad; Alanazi, Mohammed M.This work is based on the synthesis of new ether derivatives bearing benzothiazole (BTA) scaffold through multistep reaction process. Initially, BTA was prepared by refluxing 4-hydroxybenzaldehyde with aminothiophenol having sodium metabisulfite in dimethylformamide (DMF); subsequently, the product was further refluxed with different substituted benzyl and alkyl bromides in acetone to get ether hybrids of BTA in good yields. Structurally, these compounds were confirmed by means of 1H, 13C-NMR, and mass spectrometry and evaluated for in vitro thymidine phosphorylase (TP) inhibitory activity. In the series, seven compounds attributed excellent inhibition against TP enzyme better than the standard. Similarly, three compounds showed good activity, whereas two compounds were found inactive. Moreover, all these compounds showed no toxicity to normal human fibroblast cell line (BJ cell line). In addition, Gaussian calculations were performed on the 6-31++g(d,p) basis set to examine the 13 synthesized compounds at the B3LYP, HF, and M062X levels. Additionally, molecular docking calculations were performed on TP enzyme proteins (PDB IDs: 4EAD, 2WK6, and 4LHM), and absorption, distribution, metabolism, excretion/toxicity (ADME/T) calculations were performed to investigate the effects and responses of these compounds in human metabolism.Öğe Synthesis, Biological Activities, DFT Calculations, and Molecular Docking Studies of O-Methyl-Inositols(Wiley-V C H Verlag Gmbh, 2025) Aksu, Kadir; Ayvaz, Melek col; Celik, Omer Faruk; Serdaroglu, Goncagul; Ustun, Elvan; Kelebekli, LatifThe concise synthesis of O-methyl-d-inositol derivative and conduritol derivatives was obtained starting from p-benzoquinone. Spectroscopic methods have been performed for the characterization of newly synthesized compounds. Cyclitols are useful molecules with anticancer, antibiotic, antinutrient, and antileukemic activities. Inositol class molecules, known as the most important cyclitol derivatives, were examined in this study for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide radical scavenging and butyrylcholinesterase (BChE) and glycosidase inhibition activities. It was observed that compound 5, in particular, showed efficacy that competed with the standards in terms of both antioxidant activity and enzyme inhibitor potential. Additionally, compound 5 shows effective antimicrobial activity. The water-soluble characteristics and lipophilic properties of the compounds were also considered and discussed. Moreover, the quantum chemical analyses were performed in light of the DFT/B3LYP/6-311G** level computations to elucidate/compare the studied inositols' possible reactivity directions. Additionally, the interactions of the molecules were analyzed against acetylcholinesterase (AChE), peroxiredoxin 5, and DNA gyrase by molecular docking methods. Cholinesterase inhibitors have an important status as the most important drug group used in the treatment of Alzheimer's disease today. Considering the effects of inhibition of the alpha-glucosidase enzyme by inhibitors, such molecules can also be used as therapeutic components in the treatment of diabetes.Öğe Novel Aminothiazole-Chalcone Analogs: Synthesis, Evaluation Acetylcholinesterase Activity, In Silico Analysis(Wiley-V C H Verlag Gmbh, 2025) Sarikaya, Meryem Kececi; Yildirim, Suheda; Kocyigit, Umit M.; Ceylan, Mustafa; Yirtici, Umit; Eyupoglu, VolkanIn this study, novel thiazole-chalcone analogs were synthesized, and their inhibitory effects on acetylcholinesterase (AChE) were examined. In vitro enzyme activity studies were conducted to calculate IC50 values, which were found to range between 2.55 and 72.78 mu M (tacrine IC50 = 53.31 mu M). The Ki values of the compounds showing the best inhibition (6g and 6e) were calculated and compared to those of the standard substance tacrine. All compounds reduced the AChE activity. Additionally, predictions made with SwissADME indicated that all compounds complied with Lipinski's rules and possessed good oral bioavailability properties, and the inhibitory effects of compounds 6e and 6g on AChE were evaluated using molecular docking and molecular dynamics simulations (100 ns). The results showed that compounds 6e and 6g had strong and stable interactions with AChE.Öğe Reaction kinetics of molybdenum dissolution by hydrogen peroxide in acidic and alkaline solutions using tartaric acid and sodium hydroxide: A semi-empirical model with rotating disc method(Wiley, 2024) Motasim, Mahmoud; Agacayak, Tevfik; Eker, Yasin Ramazan; Aydogan, Salih; Abbaker, AhmedMolybdenum is an amphoteric metal that dissolves in both acidic and alkaline solutions. This fundamental study explores a sustainable process for the dissolution of molybdenum, focusing on the reaction kinetics in H2O2, H2O2-NaOH, and H2O2-C4H6O6 solutions. A rotating disc method was applied with the Levich's equation. Semi-empirical models with activation energy were developed for the H2O2-NaOH and H2O2-C4H6O6 solutions. The study examined the effects of rotating speed, disc surface area, temperature, H2O2, NaOH, and C4H6O6 concentrations, along with rotating speed, disc surface area, and temperature. Hydrogen peroxide significantly impacted molybdenum dissolution rates across all three solutions. The reaction order of hydrogen peroxide concentration in the H2O2 solution was greater than that of the H2O2-NaOH and H2O2-C4H6O6 solutions. The complex of molybdenum peroxo was formed in H2O2 and H2O2-NaOH solutions but decomposed at a temperature >= 50 degrees C. The activation energies were determined to be 49.90, 43.60, and 41.10 kJ/mol for the H2O2, H2O2-NaOH, and H2O2-C4H6O6 solutions. Molybdenum dissolution in H2O2-NaOH solution. imageÖğe Development of Potent Type V MAPK Inhibitors: Design, Synthesis, and Biological Evaluation of Benzothiazole Derivatives Targeting p38α MAPK in Breast Cancer Cells(Wiley-V C H Verlag Gmbh, 2025) Zoatier, Bayan; Yildiztekin, K. Gizem; Alagoz, M. Abdullah; Hepokur, Ceylan; Burmaoglu, Serdar; Algul, OztekinType V MAPK inhibitors are distinguished by their capacity to target both the ATP binding site and a specific allosteric site on the enzyme. The present work utilized in silico analysis with Maestro 13.8.135 (Schrodinger) software in conjunction with experimental investigations to enhance the antiproliferative efficacy and forecast the likely mechanism of action of benzothiazole derivatives. Approximately 28 compounds were developed, produced, and assessed for their antiproliferative properties against two breast cancer cell lines: ER+ (MCF7) and ER- (MDA-MB-231), in addition to one normal mouse fibroblast cell line (L929). Their antiproliferative activities were evaluated via the MTT test, with doxorubicin and cisplatin serving as reference drugs for comparison. Consequently, the compounds with the greatest activity against the MCF7 cell line were chosen, and their inhibitory effects on the p38 alpha MAPK enzyme were examined. The molecular docking studies of compounds 15 and 19 demonstrated significant binding affinities for p38 alpha MAPK. Molecular dynamics simulations conducted over 100 ns revealed that compounds 15 and 19 exhibit stability inside both the ATP-binding domain and the lipid domain of p38 alpha MAPK. The research focused on creating effective Type V MAPK inhibitors demonstrate that compounds 15 and 19 possess considerable ability to inhibit p38 alpha MAPK, hence establishing them as promising anticancer agents.Öğe Black Garlic Extract Modulates Endothelin Expression and Ovulatory Function in Monosodium Glutamate Treated Rats(Wiley, 2025) Gezer, Arzu; Aras, Sukran Yediel; Ozkaraca, Mustafa; Baygutalp, Nurcan Kilic; Gundogdu, Gulhande; Sari, Ebru Karadag; Bedir, GurselMonosodium glutamate (MSG), a widely used food additive, has been associated with various health concerns, including potential reproductive toxicity. This study investigated the protective effects of black garlic (BG) ethanol extract against MSG-induced ovarian damage in rats. Thirty-two female rats in estrus were randomly divided into four groups (n = 8 per group): control (saline), BG (250 mg/kg BW), MSG (4 mg/g BW), and BG+MSG (combined treatment). Treatments were administered daily for 14 days. Ovarian tissues were collected for histopathological, immunohistochemical (IHC), and biochemical analyses. Histopathological examination revealed a significant reduction in cystic follicles in the BG+MSG group compared to the MSG group (p < 0.0001). IHC analysis showed decreased immunoreactivity of endothelin-1 and endothelin-2 in the BG+MSG group compared to the MSG group (both p < 0.01). Biochemical assays demonstrated significantly increased follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol levels in the BG+MSG group compared to the MSG group (all p < 0.05), while progesterone levels were significantly lower in the MSG group compared to the BG+MSG group (p < 0.05). These findings suggest that BG ethanol extract may mitigate MSG-induced ovarian dysfunction in rats by alleviating degenerative changes in follicles and modulating hormonal levels. This study provides insights into potential natural interventions for MSG-related reproductive toxicity.Öğe Suitability of Water Quality for Irrigation Purposes Using GIS-Based Irrigation Water Quality Index(Wiley, 2025) Simsir, Mehmet; Yildiz, Sayiter; Karakus, Can Bulent; Ozbek, Dilara UlgerIn this study, surface water quality was assessed on the basis of irrigation water quality indices and the irrigation water quality index (IWQI) via GIS. The study was carried out on the basis of analyses of samples collected in August (dry) and November (wet) 2023 from 12 designated points along the Y & imath;ld & imath;z River in Sivas. The sodium adsorption ratio (SAR), Kelly index (KI), sodium percentage (Na%), permeability index (PI), residual sodium carbonate (RSC), magnesium hazard (MH) indices and IWQI were calculated to determine the classification of irrigation water quality. Additionally, analyses of Ca2+, Cl-, Fe2+, K+, HCO3-, Mg2+, Mn, Na+, pH and SO42- were conducted on the samples. The spatial distributions of the calculated parameters were mapped via GIS, and irrigation water quality assessment was performed according to the US Salinity Diagram and irrigation water quality standards. The IWQI values ranged from 401 to 61 during the rainy season and from 42 to 67 during the dry season. In the rainy season, two surface water samples were classified as 'poor (MR: moderate restriction, IWQI: 55-70)' and nine as 'very poor (HR: high restriction, IWQI: 40-55)'. In the dry season, three surface water samples were classified as 'poor (MR: moderate restriction)' and nine as 'very poor (HR: high restriction)'. According to the US Salinity Diagram, the majority of surface water samples in both the rainy and dry seasons fell into categories C3S1 (high-salinity hazard-low-sodium hazard) and C2S1 (medium-salinity hazard-low-sodium hazard), respectively. The results highlight the effectiveness of these methodologies in evaluating surface water quality, assisting in the development of informed management strategies for sustainable water resource use in agricultural environments. The IWQI has proven to be a good tool for assessing the quality of irrigation water in the study area and managing water quality and can help decision makers manage water resources more effectively for sustainable agriculture.Öğe Preserving Quality and Extending Shelf Life of Climacteric Persimmon Fruits Using Melatonin and Modified Atmosphere Packaging(Wiley, 2025) Kucuker, Emine; Gundogdu, Muttalip; Guler, Emrah; Sumbul, Ahmet; Tekin, Onur; Hallac, BulentQuality and product losses during the postharvest storage of climacteric persimmon fruits pose significant challenges due to their short shelf life and limited marketing period. This study examined the effects of melatonin (1 mM) and modified atmosphere packaging (MAP) on preserving the quality, biochemical properties, and organic acid contents of persimmons, as well as extending their shelf life during storage at 0 degrees C +/- 0.5 degrees C. The MAP resulted in the lowest weight loss, recording only 6.63%. The combination of melatonin and MAP provided the highest fruit firmness at 8.69 kg/cm2, along with a total antioxidant level of 12.40 mu mol TE 100 g-1. Additionally, this treatment improved total phenolic content and most individual organic acids, except for fumaric acid, which was highest in the MAP treatment. The organic acid contents of the fruits varied during storage, depending on the specific acid. Malic acid was the predominant organic acid and was consistently better preserved by the treatments compared to the control. It is important to note that the reduction in malic acid was approximately 1.5 times less in the treated fruits than in the controls, while the decline of other organic acids was 3-4 times greater. In conclusion, both MAP and the combination of melatonin and MAP were effective methods for preserving the quality attributes and extending the shelf life of persimmon fruits.Öğe Comprehensive evaluation of deep neck infections: A retrospective analysis of 111 cases(Wiley, 2024) Aksoy, Ahmet; Demirkiran, Berat Baturay; Bora, Adem; Dogan, Mansur; Altuntas, Emine ElifPurpose: Our study will analyze patients' clinical characteristics, treatment strategies, and complications with deep neck infection (DNI) using their medical records for five years. Methods: The present study included 111 patients diagnosed with DNI in our clinic between January 2018 and March 2023. The patients' complaints at admission, sociodemographic characteristics, season of diagnosis, findings from laboratory tests, radiological imaging performed at the time of first diagnosis, abscess localization, medical and surgical treatment methods used, and complications developing during follow-up were retrospectively examined. Results: The mean age of the patients included in the study was 38.51 +/- 16.92 (6-87 years). There was a significant correlation between chronic disease, smoking behaviours, oral hygiene, and DNI among patients based on their sociodemographic characteristics and medical history (p < .005). DNI development did not differ by season (p > .005). Physical examination findings predominantly revealed neck masses (39.6%) and peritonsillar abscesses (32.4%), and patients with peritonsillar abscesses had a shorter length of hospital stay than those with other localizations. No severe complications occurred during the clinical follow-up. Conclusion: Chronic diseases, smoking, and poor oral hygiene are the primary risk factors for developing DNIs. If an abscess is located in a critical area, it may require extended hospitalization and surgery under general anesthesia. Therefore, addressing these risk factors and encouraging good oral hygiene practices are crucial to preventing DNIs and reducing the need for intensive treatment.Öğe Investigation of Long Noncoding RNA-NRAV and Long Noncoding RNA-Lethe Expression in Crimean-Congo Hemorrhagic Fever(Wiley, 2024) Baysal, Aysenur Comez; Kiymaz, Yasemin cakir; Sahin, Nil Ozbilum; Bakir, MehmetCrimean-Congo hemorrhagic fever (CCHF) is a zoonotic infectious disease caused by the CCHF virus, a member of the Bunyavirales order and the Orthonairoviridae family. The exact pathogenesis is not fully understood. Long noncoding RNAs (lncRNAs) are RNAs that are shown to play a role in various pathological processes of viral diseases. NRAV and Lethe are two well-known lncRNAs. Although previous studies have shown that NRAV and Lethe play important roles in the pathogenesis of viral infections, their role in CCHF is unknown. This study aimed to evaluate the expression levels of NRAV and Lethe in patients with CCHFV. Eighty patients diagnosed with CCHF were included, and RNA was extracted from their blood samples. The expression of NRAV and Lethe was measured using quantitative real-time polymerase chain reaction (qPCR). Patients were divided into three groups based on severity score, which was mild, moderate, and severe, and into two groups (survivors and non-survivors). The expression levels of NRAV and Lethe were compared between these groups. Of the patients, 49 (61.25%) were male, 31 (38.75%) were female, and the mean age was 38.62 +/- 19.28 years. No differences in age or gender were found between the groups. It was shown that NRAV expression was 21.86 times higher in the severe patient group compared to the moderate group and 22.74 times higher than in the mild group, statistically significant. When comparing fatal cases with survivors, NRAV expression levels were found to be 9.2 times higher in fatal cases. Lethe levels were 3 times lower in moderately severe cases compared to mild cases, but this difference was not statistically significant. In conclusion, our study suggests that NRAV may be a lncRNA involved in the pathogenesis of CCHFV.Öğe SIRT2 deacetylates and decreases the expression of FOXM1 in colon cancer(Wiley, 2024) Yildiz, Baris; Demirel, Ramazan; Staudacher, Jonas J.; Beseren, Hatice; Yildiz, Gulden; Akpinar, Ali Emre; Park, Seong-HoonNew FOXM1-specific inhibitors with the potential to be used for therapeutic purposes are under extensive research. We hypothesized that deacetylation of FOXM1 would decrease protein expression, thus providing novel therapeutic management of colon cancers. Immunostaining was used to determine FOXM1 and SIRT2 expressions in human colon cancer tissue microarrays (n = 90) from Stage I to Stage IV. SIRT2-FOXM1 interaction was evaluated in colon cancer cells using immunoprecipitation. Deacetylation of FOXM1 via SIRT2 was determined using in vitro deacetylation assays. FOXM1 could be hyper-acetylated when p300 and pCAF histone acetyltransferases were administered alongside deacetylase inhibitors. We detected that SIRT2 and FOXM1 physically interacted, and SIRT2 deacetylated FOXM1 in vitro. SIRT2 overexpression led to a significant decrease while knockdown of SIRT2 increased the FOXM1 expression in HCT116 human colon carcinoma cells. In the analysis of 90 human colorectal cancer samples, high SIRT2 expression was observed in about 49% of colorectal cancer, intermediate in 29%, and low or no staining in 22%. Strong SIRT2 expression was found to be negatively associated with the FOXM1 staining in our clinical cohort. This study reveals a molecular interaction and association between SIRT2 and FOXM1 expression in colon cancer cell lines and human colon cancer samples, and suggests that targeting SIRT2 activity using small molecule modulators may be a promising therapeutic approach for colorectal cancer. SIRT2-FOXM1 interaction in colon cancer. SIRT2 and FOXM1 physically interacted and SIRT2 deacetylated FOXM1 in vitro. Strong SIRT2 expression was negatively associated with FOXM1 staining in a human colon carcinoma tissue microarray. Resveratrol, berberine, and quercetin reduced FOXM1 expression in colon cancer cells.image Upregulation of FOXM1 may be an early molecular signal required for cancer SIRT2 and FOXM1 physically interacted, and SIRT2 deacetylated FOXM1 in vitro Strong SIRT2 expression was negatively associated with the FOXM1 staining in the TMA Resveratrol, berberine and quercetin decreased FOXM1 expression in colorectal cancer cellsÖğe In Vitro Neuroprotective Effect Evaluation of Donepezil-Loaded PLGA Nanoparticles-Embedded PVA/PEG Nanofibers on SH-SY5Y Cells and AP-APP Plasmid Related Alzheimer Cell Line Model(Wiley-V C H Verlag Gmbh, 2025) Guler, Ece; Yekeler, Humeyra Betul; Uner, Burcu; Dogan, Murat; Asghar, Asima; Ikram, Fakhera; Yazir, YusufhanRecently developed nanoparticles and nanofibers present new brain-specific treatment strategies, especially for Alzheimer's disease treatment. In this study, donepezil (DO)-loaded PLGA nanoparticles (DNP) are embedded in PVA/PEG nanofibers (DNPF) produced by pressurized gyration for sublingual administration. SEM images showed produced drug-loaded and pure nanofibers, which have sizes between 978 and 1123 nm, demonstrated beadless morphology and homogeneous distribution. FT-IR, XRD, and DSC results proved the produced nanoparticles and fibers to consist of the DO and other polymers. The in vitro drug release test presented that the release profile of DO is completed at the end of the 18th day. It is released by the first order kinetic model. DNPF has an ultra-fast release profile via its disintegration within 2 sec, which proved itself to be suitable for the administration sublingually. All samples presented above approximate to 90% cell viability via their non-toxic natures on SH-SY5Y human neuroblastoma cells by using Alamar blue assay. The anti-Alzheimer effects of DO, DNP, and DNPF are evaluated on the A beta(1-42)-induced SH-SY5Y cells at 1, 5, and 10 mu M as treatment groups. The 1 mu M dosage exhibited the most significant neuroprotective effects, which showed enhanced cellular uptake and superior modulation of Alzheimer's-related proteins, including tau and A beta.Öğe Moxifloxacin-Impregnated Contact Lenses for Treatment of Keratitis in Rabbit Eyes(Wiley, 2025) Erdogan, Hakika; Gungor, Buket; Suner, Selin S.; Silan, Coskun; Saraydin, Serpil U.; Saraydin, Dursun; Ayyala, Ramesh S.Moxifloxacin (MOX) was loaded into commercial contact lenses (CLs) via supercritical carbon dioxide (ScCO2) to attain MOX-impregnated CL for keratitis treatment. This study aimed to investigate Pseudomonas keratitis treatment with MOX-impregnated CL compared to the traditional eyedrop administration. MOX impregnation was accomplished employing optimum parameters of 2.5 h drug exposure time, 25 MPa pressure, and 313 K for ScCO2 conditions using ethanol co-solvent rendering sustainable delivery, up to 7 days at effective dosage formulation. The MOX-impregnated CL was found to be safe with no significant toxicity on fibroblast cells after 5 days of contact time. Bacterial viability in vivo keratitis treatment in rabbit eyes was significantly decreased to 10(2) from 10(9) CFU/cornea for MOX-impregnated CL treatment, almost similar to exhaustive conventional 0.5% MOX eye drop treatments. The MOX-impregnated CL treatment revealed no conjunctival hyperemia, edema, or secretion for all eyes in the relevant group, and transparent cornea with no keratitis focus was obtained for two of the eyes (n = 6). The normal histological structure was seen with MOX-impregnated CL treatment on healthy eyes. Moreover, polymorphonuclear cell infiltration observed in keratitis eyes without any treatment was significantly decreased to a few polymorphonuclear cells in the groups treated with MOX eyedrops and MOX-impregnated CL.Öğe Validity of a nutrition screening tool for childhood cancer(Wiley, 2024) Kangalgil, Melda; Meral, Buket; Alford, Alexia J. Murphy; Erduran, ErolBackgroundMalnutrition in pediatric oncology patients is a serious clinical condition. There is a need for standardized nutrition screening in pediatric oncology patients, as nutrition screening can offer a simple method to identify children with cancer at risk of malnutrition. This study aimed to determine the validity of a Turkish version of nutrition screening tool for childhood cancer (SCAN) in identifying the risk of malnutrition among children with cancer.Materials and MethodsA cross-sectional study was conducted with 78 children with cancer admitted to the pediatric hematology-oncology unit of a university hospital. In the first stage of this study, SCAN was translated into Turkish, and in the second stage, the validity of SCAN against pediatric Subjective Global Nutritional Assessment (SGNA) and Academy of Nutrition and Dietetics/American Society for Parenteral and Enteral Nutrition (AND/ASPEN) malnutrition criteria were evaluated.ResultsPatients had a median age of 8.0 years (range, 2-18 years; IQR, 5-14 years), 61.5% were male, and 60.3% were diagnosed with leukemia. According to SCAN, 53.8% had high risk of malnutrition. Validation of SCAN against pediatric SGNA showed that SCAN has a sensitivity of 97.5%, specificity of 94.5%, and accuracy of 96.1%.ConclusionThe risk of malnutrition is common in children with cancer. The Turkish version of the SCAN is a simple, quick, and valid tool to determine the risk of malnutrition in children with cancer. Further research is needed to understand the impact of nutrition interventions on clinical outcomes in children at risk for malnutrition based on SCAN.Öğe Middle School Students' Motivations and Learning Competencies in Science: Mediating Role of Digital Literacy(Wiley, 2025) Nacaroglu, Oguzhan; Kizkapan, Oktay; Demir, HuseyinScience learning motivation refers to the drive and enthusiasm that students exhibit towards learning science. Science learning competencies, on the other hand, are the skills and knowledge enabling students to understand and apply scientific concepts effectively. Digital literacy, defined as the ability to effectively use digital tools and resources, has emerged as a crucial skill in modern education. Digital literacy can bridge the gap between motivation and competencies by providing students with the tools they need to engage more deeply with scientific content. Given its significance, exploring the mediating role of digital literacy in the relationship between science learning motivation and competence is essential. Hence, we aimed to test the mediating role of digital literacy in the relationship between middle school students' science learning motivation and science learning competencies. To test these relations, we adopted the correlation design of the quantitative research method. The sample consisted of 471 middle school students. Science learning motivation scale, competence scale for learning science, and digital literacy scale were used to collect data. The hypothesis model was tested by using covariance-based structural equation modeling (CB-SEM). The results showed that science learning motivation is correlated with students' science learning competency (r = 0.891, p < 0.05) and digital literacy (r = 0.538, p < 0.05). Also, digital literacy partially mediates the relation between science learning motivation and science learning competency (r = 0.757, p < 0.05). These findings highlight the importance of fostering digital literacy in educational settings to enhance students' motivation and competency in science learning. Educators and policymakers should integrate digital tools and resources into the curriculum to create a more engaging and effective learning environment, thereby bridging the gap between students' motivation and their ability to master scientific concepts.Öğe Next-Generation Application-Based Artificial Intelligence in Modeling and Estimation for Ni/n-GaAs/In Schottky Barrier Diode(Wiley-V C H Verlag Gmbh, 2023) Dogan, Hulya; Kockanat, SerdarHerein, for Ni/n-GaAs/In Schottky barrier diode, experimental measurement, modeling, data generation from the model, and parameter estimation processes are simultaneously carried out. In the experimental step, Ni/n-GaAs/In Schottky barrier diodes are fabricated and annealed from the temperature of 200 degrees C up to 600 degrees C with 100 degrees C steps. Current values are recorded by applying voltage to the diode contacts from -1 V up to 0.5 V. In the modeling step, 1503 experimental current-voltage data are used for 19 different regression models. For Adaptive Neuro Fuzzy System (ANFIS), when root mean square error, mean square error, mean absolute error, and coefficient of determination are calculated 6.0341e-07, 3.6410e-13, 2.3873e-07, and 0.9999 for training, they are obtained 5.8904e-07, 3.4697e-13, 2.3083e-07, and 0.9999 for testing. In the estimation step, the values of electrical parameters are estimated by using Mayfly algorithm. Estimations are performed for all annealing temperatures. In addition, current-voltage data for the annealing temperature of 350 degrees C are produced by the ANFIS model. Thus, a new-generation artificial intelligence application, that includes measurement, modeling, and estimation for the Ni/n-GaAs/In Schottky barrier diode with varying annealing temperatures, is realized and a new perspective is provided to researchers and practitioners.Öğe Sulfonamide-Bearing Pyrazolone Derivatives as Multitarget Therapeutic Agents: Design, Synthesis, Characterization, Biological Evaluation, In Silico ADME/T Profiling and Molecular Docking Study(John Wiley & Sons Ltd, 2025) Lolak, Nebih; Akocak, Suleyman; Topal, Meryem; Kocyigit, Umit Muhammet; Isik, Mesut; Turkes, Cuneyt; Topal, FevziThe research and design of new inhibitors for the treatment of diseases such as Alzheimer's disease and glaucoma through inhibition of cholinesterases (ChEs; acetylcholinesterase, AChE and butyrylcholinesterase, BChE) and carbonic anhydrase enzymes are among the important targets. Here, a series of novel sulfonamide-bearing pyrazolone derivatives (1a-f and 2a-f) were successfully synthesized and characterized by using spectroscopic and analytical methods. The inhibitory activities of these newly synthesized compounds were evaluated both in vitro and in silico for their effect on carbonic anhydrases (hCA I and hCA II isoenzymes) and ChEs. The in vitro studies showed that these novel compounds demonstrated potential inhibitory activity, with K-I values covering the following ranges: 18.03 +/- 2.86-75.54 +/- 4.91 nM for hCA I, 24.84 +/- 1.57-85.42 +/- 6.60 nM for hCA II, 7.45 +/- 0.98-16.04 +/- 1.60 nM for AChE, and 34.78 +/- 5.88-135.70 +/- 17.39 nM for BChE. Additionally, many of these compounds showed promising inhibitory activity, and some showed higher potency than reference compounds. While the in silico studies have also identified the potential binding positions of these compounds, using the crystal structures of hCA I, II, AChE and BChE receptors. The varying affinities demonstrated by these designed compounds for ChEs and hCA isoenzymes show that these compounds could hold promise as potential alternative agents for selectively inhibiting ChEs and hCAs in the treatment of diseases such as Alzheimer's disease and glaucoma.
