Molecularly imprinted composite discs for transferrin recognition
| dc.authorid | 0000-0003-0900-035X | tr |
| dc.contributor.author | Aylaz, Gülgün | |
| dc.contributor.author | Zenger, Okan | |
| dc.contributor.author | Baydemir Peşint, Gözde | |
| dc.contributor.author | Andaç, Müge | |
| dc.date.accessioned | 2023-04-11T05:47:01Z | |
| dc.date.available | 2023-04-11T05:47:01Z | |
| dc.date.issued | 2022 | tr |
| dc.department | Mühendislik Fakültesi | tr |
| dc.description.abstract | Human Transferrin (HTr) imprinted composite cryogel (HTr-MIPCC) discs were synthesized with distinctive structure and increased adsorption capacity and specificity for HTr by combining the advantages of the surface imprinting technique. HEMA-based cryogel was embedded with HTrsurface imprinted particles. Thanks to the imprinted particles embedding, both the higher surface area and specific recognition are provided. The structure of the HTr-MIPCC was characterized by FTIR Spectrometer, SEM, swelling studies, flow dynamics, and surface area measurements. The addition of surface imprinted particles into the cryogel disc has resulted in an increased surface area, enhancing the capacity of composite cryogel for HTr adsorption up to 10.96 mg/g and gel fractionation yields reached up to 83%. Selectivity studies were carried out against HIgG and HSA as competitors. The HTr-MIPCC discs were packaged into an FPLC column for selective depletion of HTr. It was calculated that the HTr-MIPCC is 1.071 and 1.062 times selective for HTr than HSA and HIgG, respectively. According to FPLC studies HTr-MIPCC can be considered as an excellent alternative for affinity matrixes used for the detection and selective recognition of HTr directly from human serum. HTr-MIPCC discs can be used several times without any noteworthy reduction in the adsorption capacity of HTr | tr |
| dc.description.sponsorship | This project was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) within the scope of 3001-Initial R&D Projects Support Program [project number 114Z664]. | tr |
| dc.identifier.citation | Gülgün Aylaz, Okan Zenger, Gözde Baydemir Peşint & Müge Andaç | tr |
| dc.identifier.endpage | 1375 | tr |
| dc.identifier.issue | 9 | tr |
| dc.identifier.startpage | 1359 | tr |
| dc.identifier.uri | https://hdl.handle.net/20.500.12418/13484 | |
| dc.identifier.volume | 57 | tr |
| dc.language.iso | en | en_US |
| dc.publisher | Taylor and Francis | tr |
| dc.relation.ispartof | Seperation Science and Technology | en_US |
| dc.relation.publicationcategory | Uluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanı | tr |
| dc.rights | info:eu-repo/semantics/openAccess | tr |
| dc.subject | Molecular imprinting | tr |
| dc.subject | composite discs | tr |
| dc.subject | chromatography application | tr |
| dc.subject | transferrin | tr |
| dc.subject | protein recognition | tr |
| dc.title | Molecularly imprinted composite discs for transferrin recognition | en_US |
| dc.type | Article | en_US |
