Electrochemical DNA Sensor Designed Using the Pencil Graphite Electrode to Detect Listeria monocytogenes

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dc.contributor.authorKüçükoğlu, Nurçin
dc.contributor.authorKıvanç, Merih
dc.contributor.authorŞahin, Yücel
dc.date.accessioned2025-05-07T17:12:21Z
dc.date.available2025-05-07T17:12:21Z
dc.date.issued07.2024
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Beslenme Ve Diyetetik Bölümü
dc.description.abstractIn the present work, a novel electrochemical DNA sensor was designed to detect L. monocytogenes. Two different gene fragments were selected for the sensor design. One is a 702 bp long fragment of the hlyA gene, encoding the synthesis of listeriolysin O toxin, which is unique only to pathogenic strains of L. monocytogenes and is essential for pathogenicity. The other is a 209 bp long fragment of the 16 S RNA gene found in all species of the Listeria genus. As the working electrode, the pencil graphite electrode was modified in various ways (activated or covered with polypyrrole), and six different combinations were constituted using three types of the modified working electrode and two different gene fragments. The developed system is based on differential pulse voltammetric transduction of guanine oxidation after hybridization between the selected gene fragment (38 µg/mL) and the selected fragment-specific inosine-modified probe (1.8 µmol/L) immobilized on a pencil graphite electrode surface. The comparison of all combinations demonstrates that the best results are obtained with the combination formed from a polypyrrole-coated pencil graphite electrode (prepared at 2 scans) and 702 bp fragment of the hlyA gene. The analysis time is less than 1 hour, and the necessary DNA concentrations for the analysis have been determined as 8.2 × 10−11 M DNA and 2.7 × 10−10 M DNA respectively, for the hlyA gene and 16 S RNA gene.
dc.identifier.doi10.1007/s12010-023-04746-w
dc.identifier.endpage4698
dc.identifier.issn0273-2289
dc.identifier.issue7
dc.identifier.pmid37843717
dc.identifier.startpage4679
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35723
dc.identifier.volume196
dc.identifier.wosWOS:001166950700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.institutionauthorDinçer, Emine
dc.institutionauthoridhttps://orcid.org/0000-0002-6361-4419
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGenosensors · Listeriolysin O toxin · DNA hybridization · Polypyrrole · Pencil graphite electrode
dc.titleElectrochemical DNA Sensor Designed Using the Pencil Graphite Electrode to Detect Listeria monocytogenes
dc.typeArticle

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