Sugammadex Causes C6 Glial Cell Death and Exacerbates Hydrogen Peroxide-Induced Oxidative Stress

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dc.contributor.authorŞahin, Bilal
dc.contributor.authorKarabulut, Sabahattin
dc.date.accessioned2025-05-04T16:26:29Z
dc.date.available2025-05-04T16:26:29Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjective: Sugammadex (SUG) quickly reverses steroidal neuromuscular blocking drugs after anesthesia. It has been reported that SUG has a toxic effect on neurons in primary culture. This study aims to examine the effect of SUG on glial cell viability, oxidative stress, and apoptosis in C6 glial cells after exposure to H2O2.Method: In this study, C6 glioma cell line was used to study the effect of SUG on the glial cell in four cell groups. The control group was untreated. Cells in the H2O2group were treated with 0.5 mM H2O2 for 24 h. Cells in the SUG group were treated with 50 μg/mL SUG for 24 h. Cells in the SUG+ H2O2 group were pre-treated with 50 μg/mL of SUG for 1 h before 24-h exposure to 0.5 mM H2O2. Cell viability was evaluated using XTT assay. Total antioxidant status (TAS), total oxidant status (TOS), caspase-3, Bax, 8-hydroxy-2′ -deoxyguanosine (8-OHdG), and cleaved-PARP levels in the cells were measured by commercial kits. Results: SUG significantly decreased the viability of C6 cells after H2O2-induced oxidative stress (p < 0.05). SUG pretreatment also raised TOS levels and led to increased Bax, Caspase-3, 8-OHdG, and cleaved PARP levels after H2O2-induced oxidative damage in C6 cells (p < 0.05).
dc.description.abstract[No abstract available]
dc.identifier.doi10.7197/cmj.1069629
dc.identifier.doihttps://doi.org/10.7197/cmj.1069629
dc.identifier.endpage27
dc.identifier.issn1305-0028
dc.identifier.issue1
dc.identifier.startpage22
dc.identifier.urihttps://hdl.handle.net/20.500.12418/32737
dc.identifier.volume44
dc.language.isoen
dc.publisherSivas Cumhuriyet University
dc.relation.ispartofCumhuriyet Tıp Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250504
dc.subjectsuggammadex
dc.subjectcell death
dc.subjectC6 glioma
dc.titleSugammadex Causes C6 Glial Cell Death and Exacerbates Hydrogen Peroxide-Induced Oxidative Stress
dc.typeResearch Article

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