Evaluated periodontal tissues and oxidative stress in rats with  neuropathic pain-like behavior

Toraman, Ayşe; Toraman, Emine; Özkaraca, Mustafa; Budak, Harun

Evaluated periodontal tissues and oxidative stress in rats with neuropathic pain-like behavior

dc.contributor.author	Toraman, Ayşe
dc.contributor.author	Toraman, Emine
dc.contributor.author	Özkaraca, Mustafa
dc.contributor.author	Budak, Harun
dc.date.accessioned	2024-03-04T10:56:26Z
dc.date.available	2024-03-04T10:56:26Z
dc.date.issued	Ekim 2023	tr
dc.department	Eğitim Bilimleri Enstitüsü	tr
dc.description.abstract	Background Oxidative stress has a critical effect on both persistent pain states and periodontal disease. Voltage-gated sodium NaV1.7 (SCN9A), and transient receptor potential ankyrin 1 (TRPA1) are pain genes. The goal of this study was to investi gate oxidative stress markers, periodontal status, SCN9A, and TRPA1 channel expression in periodontal tissues of rats with paclitaxel-induced neuropathic pain-like behavior (NPLB). Methods and results Totally 16 male Sprague Dawley rats were used: control (n=8) and paclitaxel-induced pain (PTX) (n=8). The alveolar bone loss and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were analyzed histometrically and immu nohistochemically. Gingival superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities (spectrophotometric assay) were measured. The relative TRPA1 and SCN9A genes expression levels were evaluated using quantitative real-time PCR (qPCR) in the tissues of gingiva and brain. The PTX group had significantly higher alveolar bone loss and 8-OHdG compared to the control. The PTX group had significantly lower gingival SOD, GPx and CAT activity than the control groups. The PTX group had significantly higher relative gene expression of SCN9A (p=0.0002) and TRPA1 (p=0.0002) than the control in gingival tissues. Increased nociceptive susceptibility may affect the increase in oxidative stress and periodontal destruction. Conclusions Chronic pain conditions may increase TRPA1 and SCN9A gene expression in the periodontium. The data of the current study may help develop novel approaches both to maintain periodontal health and alleviate pain in patients suffering from orofacial pain.	tr
dc.identifier.uri	https://hdl.handle.net/20.500.12418/14577
dc.language.iso	en	en_US
dc.relation.publicationcategory	Uluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanı	tr
dc.rights	info:eu-repo/semantics/openAccess	tr
dc.subject	Chronic pain, Oxidative stress, Nociceptive sensitivity, Periodontal disease, SCN9A, TRPA1	tr
dc.title	Evaluated periodontal tissues and oxidative stress in rats with neuropathic pain-like behavior	en_US
dc.type	Article	en_US