Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri

Amaç: Bu çalmada yurdumuzda çeitli hastalklarn geleneksel tedavisinde kullanlan kekik Thymus vulgaris , kimyon Cuminum cyminum ve mersin Myrtus communis bitkilerinden elde edilen ya larn Leishmania promastigotlarnn canll üzerine etkilerinin belirlenmesi amaçlanmtr. Yöntem: Bitkilerden elde edilen ya lar 2/10 ile 1/100 arasnda de ien oranlarda seyreltilerek çalmaya alnmtr. 2.107 promastigot/ml Leishmania solüsyonundan alnan 100’er l mikrotitrasyon plaklarna da tlm ve üzerlerine farkl oranlarda RPMI ile seyreltilen bitkisel ya lardan 100’ er l konularak 20 dakika beklenmitir. Ik mikroskobu altnda Thoma lamnda hareketli ve hareketsiz promastigotlar saylarak canllk yüzdeleri hesaplanmtr. Bulgular: Aratrlan bitkisel ya lardan kekik ya en yüksek antileishmanial etkiyi gösterirken LD50= 1/1200 ml/ml , LD50 de eri mersin ya için 1/1800 ml/ml ve kimyon ya için 1/3000 ml/ml olarak tespit edilmitir. Sonuç: Leishmaniasis ülkemizde görülen önemli paraziter hastalklardan biridir. Leishmaniasisin tedavisinde yaanan zorluklar nedeniyle alternatif ilaç araylar gündemdedir. leri çalmalara gereksinim duyulmakla birlikte, bu çalmada denenen bitkisel ya lardan özellikle kekik ya nn leishmaniasis tedavisinde kullanlabilece i düünülmektedir.

Objective: In this study, it is aimed to determine the in vitro antileishmanial activity of essential oils obtained from three different plants such as Thyme Thymus vulgaris , Cummin Cuminum cyminum and Mersin Myrtus communis that widely used in traditional medicine in our country. Method: Essential oils from these plants were diluted ranging from 2/10 to 1/100. 100 l of Leishmania promastigote solution 2.107 promastigot/ml dispensed in microtitration plate wells and 100 l plant essantial oil diluted in RPMI with different ratios was added onto, afterwards waited for 20 minutes. The motile and non-motile promastigotes were counted on Thoma slide under light microscope and percentage of cell viability was assessed.Results: The highest in vitro antileishmanial activity against promastigote forms of the parasite was obtained with Thyme oil LD50= 1/1200 ml/ml . LD50 doses of Cumin oil and Mersin oil were 1/ 1800 ml/ml and 1/3000 ml/ml, respectively. Conclusion: Because of the diffuculties of the treatment of leishmaniasis which is one of the most important parasitic diseases in Turkey, new drug researches are concerned, recently. In this study, we observed that particularly Thyme oil can be used for the treatment of Leishmaniasis, after further researches are performed.