Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Basit Öğe Kaydı
dc.contributor.authorKarakaş, Emin Ufuk
dc.contributor.authorPektaş, Ayşe Nur
dc.contributor.authorBerk, Şeyda
dc.date.accessioned2025-05-04T16:18:49Z
dc.date.available2025-05-04T16:18:49Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractReverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes. It is very important to normalize according to stably expressed housekeeping genes in order to facilitating gene expression studies and to acquire exact and meaningful results. The purpose of this study was to identify and validate six housekeeping genes (GADPH, RPS18, α-TUB, EF1α, ArgK and ACTB) in adults of cockroach species Blaptica dubia employing five different algorithms (geNorm, Bestkeeper, Normfinder, ΔCt method and RefFinder) to assess putative housekeeping gene expression stability. Our study also showed that the geNorm, Normfinder ΔCt method and RefFinder algorithms identified GADPH as the most stable housekeeping gene in B. dubia adults. Additioanlly, RPS18 was suggested as the most stable gene by GeNorm and BestKeeeper. ACTB has been shown to be by far the least stable of all algorithms. In addition, since there are few validation studies for reference genes in cockroaches in the literature, it is considered that it would be beneficial to increase the number of studies related with RT-qPCR on the reference genes validation under biotic and abiotic conditions in cockroaches.
dc.description.abstract[No abstract available]
dc.identifier.doi10.17776/csj.1069230
dc.identifier.doihttps://doi.org/10.17776/csj.1069230
dc.identifier.endpage182
dc.identifier.issn2587-2680
dc.identifier.issn2587-246X
dc.identifier.issue2
dc.identifier.startpage176
dc.identifier.urihttps://hdl.handle.net/20.500.12418/31145
dc.identifier.volume43
dc.language.isoen
dc.publisherSivas Cumhuriyet University
dc.relation.ispartofCumhuriyet Science Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250504
dc.subjectBlaptica dubia
dc.subjectRT-qPCR
dc.subjectReference genes
dc.subjectValidation
dc.subjectNormalization.
dc.titleSelection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)
dc.typeResearch Article

Dosyalar

Koleksiyon

DergiPark