OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY

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dc.contributor.authorAlpan, Aysan Lektemür
dc.contributor.authorToker, Hülya
dc.contributor.authorÖzer, Hatice
dc.date.accessioned2025-05-04T16:18:32Z
dc.date.available2025-05-04T16:18:32Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractAmaç: Diabetes mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone have several actions such as antimicrobial ,immunostimulating, antihypoxic effects, and activates antioxidant enzymes and angiogenesis. The aim of this study is to investigate the effect of gaseous ozone on bone healing in diabetic rat calvarial defects treated with autogenous bone graft, morphometrically and immunohistochemicallyGereç ve Yöntemler:Diabetes was induced and critical size defects were created on rats. Study groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation day and the following 2 weeks daily (140ppm @ 2L/d, 2.24 mg). Total bone area was measured. Osteocalcin and Bone morphogenic protein-2  protein expressions were evaluated.Bulgular:Control and Control+Ozone groups had no osteoclast and residuel lacunae during the study . Osteoblasts in AG+Ozone group were higher than AG group at 4th week (p>0.05). AG+Ozone group had more total bone area than AG group at 4th week. AG+Ozone group revealed more BMP-2 immune positivity compared to the other groups. Osteocalcin immune positivity in AG groups was higher than those of the Control groups.Sonuçlar:Anahtar Kelimeler:
dc.description.abstractBackround: Diabetes mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone have several actions such as antimicrobial ,immunostimulating, antihypoxic effects, and activates antioxidant enzymes and angiogenesisAim: The aim of this study is to investigate the effect of gaseous ozone on bone healing in diabetic rat calvarial defects treated with autogenous bone graft, morphometrically and immunohistochemically.Material and Methods: Diabetes was induced and critical size defects were created on rats. Study groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation day and the following 2 weeks daily (140ppm @ 2L/d, 2.24 mg). Total bone area was measured. Osteocalcin and Bone morphogenic protein-2  protein expressions were evaluated.Results: Control and Control+Ozone groups had no osteoclast and residuel lacunae during the study . Osteoblasts in AG+Ozone group were higher than AG group at 4th week (p>0.05). AG+Ozone group had more total bone area than AG group at 4th week. AG+Ozone group revealed more BMP-2 immune positivity compared to the other groups. Osteocalcin immune positivity in AG groups was higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozone application decreased osteoclast number and increased osteoblast number and bone regeneration, especially, in early stages of bone regeneration in diabetic rats. KEYWORDS: autograft, bone regeneration, calvarial defect, experimental diabetes mellitus, ozone
dc.identifier.doi10.15311/selcukdentj.419582
dc.identifier.doihttps://doi.org/10.15311/selcukdentj.419582
dc.identifier.endpage53
dc.identifier.issn2148-7529
dc.identifier.issue1
dc.identifier.startpage44
dc.identifier.urihttps://hdl.handle.net/20.500.12418/31010
dc.identifier.volume7
dc.language.isoen
dc.publisherSelcuk University
dc.relation.ispartofSelcuk Dental Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250504
dc.subjectautograft
dc.subjectbone regeneration
dc.subjectcalvarial defect
dc.subjectexperimental diabetes mellitus
dc.titleOZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY
dc.typeResearch Article

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