Investigation of anti-epileptic mechanisms of 5HT1A receptor with pentylenetetrazole induced epilepsy model in rats

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dc.contributor.authorŞahin, Bilal
dc.contributor.authorÖzdemir, Ercan
dc.contributor.authorTaşkıran, Ahmet Şevki
dc.contributor.authorGümüş, Erkan
dc.contributor.authorErgül, Mustafa
dc.date.accessioned2025-05-04T16:26:42Z
dc.date.available2025-05-04T16:26:42Z
dc.date.issued2019
dc.departmentSivas Cumhuriyet Üniversitesi
dc.descriptionT739
dc.description.abstractObjective: According to current neurophysiological evidence, the role of 5-hydroxytryptamine (5-HT) receptors in epileptic seizure formation is still not fully elucidated. The aim of this study was to investigate the effects of 5-HT1A receptor on epileptic seizure with pentylenetetrazole induced epilepsy in rats. Method: In this study, 28 male Wistar Albino rats weighing 240-260 g were used. Pentylenetetrazole (PTZ, 35 mg/kg, i.p.) was injected to the rats to induce epilepsy and seizure stages were determined according to the Racine scale. Electrodes were placed in the skulls of the animals under stereotaxis for ECoG recording. All the experimental animals were sacrificed by decapitation after ECoG and video recordings. GABA level was measured using the Elisa kit from brain tissues, and c-Fos expression was shown immunohistochemically. Results: According to the results, it was shown that 8-OH-DPAT increased the time of initial myoclonic jerk (FMJ (p<0.05). However, the number of epileptic spikes was reduced by 8-OH-DPAT (p<0.05). GABA levels decreased in PTZ group (p<0.05). 8-OH-DPAT and WAY-100135 decreased c-Fos expression in all hippocampal areas (p<0.05). Conclusions: In conclusion, 5-HT1A receptor agonist 8-OH-DPAT, showed an anti-epileptic effect. The anti-epileptic effects of 5-HT1A receptor were found to be inconsistent with changes in GABA level. c-Fos expression is a marker of neuronal activation and may be related to the anti-convulsive effect of 5-HT1A receptor.
dc.description.abstract[No abstract available]
dc.description.sponsorshipSivas Cumhuriyet Üniversitesi
dc.identifier.doi10.7197/cmj.vi.623515
dc.identifier.doihttps://doi.org/10.7197/cmj.vi.623515
dc.identifier.endpage499
dc.identifier.issn1305-0028
dc.identifier.issue3
dc.identifier.startpage490
dc.identifier.urihttps://hdl.handle.net/20.500.12418/32870
dc.identifier.volume41
dc.language.isoen
dc.publisherSivas Cumhuriyet University
dc.relation.ispartofCumhuriyet Tıp Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250504
dc.subjectEpilepsy
dc.subjectpentylenetetrazole
dc.subjectserotonin
dc.subjectGABA
dc.subjectc-Fos
dc.subjectserotonin receptor agonist
dc.titleInvestigation of anti-epileptic mechanisms of 5HT1A receptor with pentylenetetrazole induced epilepsy model in rats
dc.typeResearch Article

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