dc.contributor.author | Disli, Olcay Murat | |
dc.contributor.author | Ozdemir, Ercan | |
dc.contributor.author | Berkan, Ocal | |
dc.contributor.author | Bagcivan, Ihsan | |
dc.contributor.author | Durmus, Nedim | |
dc.contributor.author | Parlak, Ahmet | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:14:09Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:14:09Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0008-4212 | |
dc.identifier.issn | 1205-7541 | |
dc.identifier.uri | https://dx.doi.org/10.1139/Y09-043 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/10086 | |
dc.description | WOS: 000270228300002 | en_US |
dc.description | PubMed ID: 19767883 | en_US |
dc.description.abstract | Radial artery (RA) vasospasm remains a potential cause of early graft failure after coronary artery bypass graft surgery, despite pretreatment with alpha-adrenergic or calcium channel blockers. Our aim was to investigate the mechanism of the vasorelaxant effects of Rho-kinase inhibitors (Y-27632 and fasudil) on the human RA. Segments were obtained from 30 patients undergoing coronary artery bypass graft and were divided into 3-4 mm vascular rings. The rings were stimulated with 10(-5) mol/L phenylephrine (PE) by using the isolated tissue bath technique and were relaxed with 10(-6) mol/L acetylcholine. Relaxation responses were recorded for Y-27632 (10(-9)-10(-4) mol/L), fasudil (10-9-10-4 mol/L), and sodium nitroprusside (SNP) (10(-9)-10(-5) mol/L). Y-27632 and fasudil relaxation responses were repeated in either N-G-nitro-L-arginine (L-NNA), which is a specific endothelial nitric oxide synthase inhibitor, or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), which is a guanylate cyclase inhibitor. SNP relaxation responses were repeated in 10(-8) mol/L Y-27632 and 10(-8) mol/L fasudil. Y-27632 and fasudil caused concentration-dependent vasorelaxation in RA rings precontracted with PE, and maximal relaxation (100%) was recorded at the highest concentration used (10(-4) mol/L). The vasorelaxant effects of Y-27632 and fasudil were significantly reduced in the presence of L-NNA and ODQ, and the pD(2) values of Y-27632 and fasudil were not changed. The vasorelaxant effects of SNP were significantly increased in the presence of Y-27632 and fasudil, and the pD(2) values of SNP were not changed. These findings indicate that Y-27632 and fasudil caused concentration-dependent vasorelaxation in the RA rings. Because this effect was decreased in a dose-dependent manner by L-NNA and ODQ, the relaxant effects of Y-27632 and fasudil could be due to stimulation by nitric oxide that is being released. Rho-kinase inhibitors may have an important role in preventing vasospasm in arterial grafts used for coronary artery surgery. | en_US |
dc.description.sponsorship | Cumhuriyet University Scientific Research Project [T-267] | en_US |
dc.description.sponsorship | This study was supported by Cumhuriyet University Scientific Research Project T-267 (CUBAP, Sivas, Turkey). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | en_US |
dc.relation.isversionof | 10.1139/Y09-043 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | radial artery | en_US |
dc.subject | vasospasm | en_US |
dc.subject | Rho-kinase | en_US |
dc.subject | Y-27632 | en_US |
dc.subject | fasudil | en_US |
dc.subject | nitric oxide | en_US |
dc.title | Rho-kinase inhibitors Y-27632 and fasudil prevent agonist-induced vasospasm in human radial artery | en_US |
dc.type | article | en_US |
dc.relation.journal | CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | en_US |
dc.contributor.department | [Ozdemir, Ercan] Cumhuriyet Univ, Sch Med, Dept Physiol, TR-58140 Sivas, Turkey -- [Disli, Olcay Murat -- Berkan, Ocal] Cumhuriyet Univ, Sch Med, Dept Cardiovasc Surg, TR-58140 Sivas, Turkey -- [Bagcivan, Ihsan -- Durmus, Nedim -- Parlak, Ahmet] Cumhuriyet Univ, Sch Med, Dept Pharmacol, TR-58140 Sivas, Turkey | en_US |
dc.identifier.volume | 87 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.endpage | 601 | en_US |
dc.identifier.startpage | 595 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |