dc.contributor.author | Akkaya, Birnur | |
dc.contributor.author | Uzun, Lokman | |
dc.contributor.author | Altintas, Evrim Banu | |
dc.contributor.author | Candan, Ferda | |
dc.contributor.author | Denizli, Adil | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:14:42Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:14:42Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1060-1325 | |
dc.identifier.issn | 1520-5738 | |
dc.identifier.uri | https://dx.doi.org/10.1080/10601320802637086 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/10244 | |
dc.description | WOS: 000262647200002 | en_US |
dc.description.abstract | Concanavalin A (Con A) immobilized monosize and magnetic poly(glycidyl methacrylate)[m-poly(GMA)] beads were investigated for specific adsorption of yeast invertase from aqueous solutions. m-Poly(GMA) beads (1.6 m in diameter) were prepared by dispersion polymerization in the presence of Fe3O4 nanopowder. The epoxy groups of m-poly(GMA) beads were opened by base catalyses. Then, Con A was immobilized by covalent binding onto the beads. Con A immobilization amount was 12.5 mg/g. The invertase adsorption capacity of the m-poly(GMA)/Con A beads was 111 mg/g. The maximum invertase adsorption on the m-poly(GMA)/Con A beads was observed at pH 5.5. The optimum activity for both free and adsorbed invertase was observed at 50C. Vmax values were determined as 330 U/mg and 292 U/mg enzyme, for free and adsorbed invertase, respectively. KM values were found to be the same for free and adsorbed invertase (20 mM). Adsorption of invertase via Con A improved the pH stability of invertase. Thermal stability also increased with adsorption. The adsorbed enzyme activity was found to be quite stable in repeated experiments. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | TAYLOR & FRANCIS INC | en_US |
dc.relation.isversionof | 10.1080/10601320802637086 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Enzyme immobilization | en_US |
dc.subject | magnetic beads | en_US |
dc.subject | poly(glycidyl methacrylate) | en_US |
dc.subject | concanavalin A | en_US |
dc.subject | invertase | en_US |
dc.title | Concanavalin a Immobilized Monosize and Magnetic Poly(glycidyl Methacrylate) Beads for Use in Yeast Invertase Adsorption | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY | en_US |
dc.contributor.department | [Uzun, Lokman -- Altintas, Evrim Banu -- Denizli, Adil] Hacettepe Univ, Dept Chem, Div Biochem, TR-06532 Ankara, Turkey -- [Akkaya, Birnur -- Candan, Ferda] Cumhuriyet Univ, Dept Chem, Sivas, Turkey | en_US |
dc.contributor.authorID | Uzun, Lokman -- 0000-0002-3971-7725 | en_US |
dc.identifier.volume | 46 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.endpage | 239 | en_US |
dc.identifier.startpage | 232 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |