Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp globosum (lamiaceae) by three different chemical assays
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This study is designed to examine the chemical composition and in vitro antioxidant activity of the essential oil and sub-fractions of the methanol extract of Marrubium globosum subsp. globosum. The GC and GC-MS analysis of the essential oil were resulted in the determination of 84 components representing 88.2% of the oil. The major constituents of the oil were spathulenol (15.8%), beta-caryophyllene (9.0%), caryophyllene oxide (7.9%), germacrene D (6.5%), and bicyclogermacrene (3.1%). Antioxidant activities of the samples were determined by three different test systems namely DPPH, beta-carotene/linoleic acid and reducing power assay. In DPPH system, the weakest radical scavenging activity was exhibited by the essential oil (1203.38 +/- 7.18 mu g ml(-1)). Antioxidant activity of the polar sub-fraction of methanol extract was superior to the all samples tested with an EC50 value of 157.26 +/- 1.12 mu g ml(-1). In the second case, the inhibition capacity (%) of the polar sub-fraction of methanol extract (97.39% +/- 0.84) was found the strongest one, which is almost equal to the inhibition capacity of positive control BHT (97.44% +/- 0.74). In the case of reducing power assay, a similar activity pattern was observed as given in the first two systems. Polar sub-fraction was the strongest radical reducer when compared with the non-polar one, with an EC50 value of 625.63 +/- 1.02 mu g ml(-1). The amount of the total phenolics was highest in polar sub-fraction (25.60 +/- 0.74 mu g/mg). A positive correlation was observed between the antioxidant activity potential and total phenolic level of the extracts. On the other hand, total flavonoid content was found equal for the both sub-fractions. (C) 2007 Elsevier Ltd. All rights reserved.