dc.contributor.author | Berkan, Ocal | |
dc.contributor.author | Bagcivan, Ihsan | |
dc.contributor.author | Kaya, Tijen | |
dc.contributor.author | Yildirim, Kemal | |
dc.contributor.author | Yildirim, Sahin | |
dc.contributor.author | Dogan, Kasim | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:16:38Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:16:38Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0008-4212 | |
dc.identifier.uri | https://dx.doi.org/10.1139/Y07-033 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/10611 | |
dc.description | WOS: 000249010900006 | en_US |
dc.description | PubMed ID: 17632587 | en_US |
dc.description.abstract | The radial artery (RA) is used as a spastic coronary bypass graft. This study was designed to investigate the mechanism of vasorelaxant effects of YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole), a nitric oxide (NO)-independent soluble guanylate cyclase (sGC activator, and DEA/NO (diethylamine/nitric oxide), a NO-nucleophile adduct, on the human RA. RA segments (n = 25) were obtained from coronary artery bypass grafting patients and were divided into 3-4 turn vascular rings. Using the isolated tissue bath technique, the endothelium-independent vasodilatation function was tested in vitro by the addition of cumulative concentrations of YC-1 (10(-10) to 3 x 10(-7) mol/L) and DEA/NO (10(-8) to 3 x 10(-5) mol/L) following vasocontraction by phenylephrine in the presence or absence of 10-5 mol/L ODQ (1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1-one), the selective sGC inhibitor, 10(-7) mol/L iberiotoxin, a blocker of Ca2+- activated K+ channels, or 10(-5) mol/L ODQ plus 10(-7) mol/L iberiotoxin. We also evaluated the effect of YC-1 and DEA/NO on the cGMP levels in vascular rings obtained from human radial artery (n = 6 for each drug). YC-1 (10(-10) to 3 x 10(-7) mol/L) and DEA/NO (10(-8) to 3 x 10(-5) mol/L) caused the concentration-dependent vasorelaxation in RA rings precontracted with phenylephrine (10(-5) mol/L) (n = 20 for each drug). Pre-incubation of RA rings with ODQ, iberiotoxin, or ODQ plus iberiotoxin significantly inhibited the vasorelaxant effect of YC-1, but the inhibitor effect of ODQ plus iberiotoxin was significantly more than that of ODQ and iberiotoxin alone p < 0.05). The vasorelaxant effect of DEA/NO almost completely abolished in the presence of ODQ and iberiotoxin plus ODQ, but did not significantly change in the presence of iberiotoxin alone (P > 0.05). The pEC(50) value of DEA/NO was significantly lower than those for YC-1 (p < 0.01), with no change E. x values in RA rings. In addition, YC- I -stimulated RA rings showed more elevation in cGMP than that of DEA/NO (p < 0.05). These findings indicate that YC-1 is a more potent relaxant than DEA/NO in the human RA. The relaxant effects of YC-1 could be due to the stimulation of the sGC and Ca2+- sensitive K(+)channels, whereas the relaxant effects of DEA/NO could be completely due to the stimulation of the sGC. YC-1 and DEA/NO may be effective as vasodilator for the short-term treatment of perioperative spasm of coronary bypass grafts. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS | en_US |
dc.relation.isversionof | 10.1139/Y07-033 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | YC-1 | en_US |
dc.subject | DEA/NO | en_US |
dc.subject | cGMP | en_US |
dc.subject | radial artery | en_US |
dc.title | Investigation of the vasorelaxant effects of 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole (YC-1) and diethylamine/nitric oxide (DEA/NO) on the human radial artery used as coronary bypass graft | en_US |
dc.type | article | en_US |
dc.relation.journal | CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | en_US |
dc.contributor.department | Cumhuriyet Univ Sch Med, Dept Cardiovasc Surg, TR-58140 Sivas, Turkey -- Cumhuriyet Univ Sch Med, Dept Pharmacol, TR-58140 Sivas, Turkey | en_US |
dc.identifier.volume | 85 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.endpage | 526 | en_US |
dc.identifier.startpage | 521 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |