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dc.contributor.authorBerkan, Ocal
dc.contributor.authorBagcivan, Ihsan
dc.contributor.authorKaya, Tijen
dc.contributor.authorYildirim, Kemal
dc.contributor.authorYildirim, Sahin
dc.contributor.authorDogan, Kasim
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:16:38Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:16:38Z
dc.date.issued2007
dc.identifier.issn0008-4212
dc.identifier.urihttps://dx.doi.org/10.1139/Y07-033
dc.identifier.urihttps://hdl.handle.net/20.500.12418/10611
dc.descriptionWOS: 000249010900006en_US
dc.descriptionPubMed ID: 17632587en_US
dc.description.abstractThe radial artery (RA) is used as a spastic coronary bypass graft. This study was designed to investigate the mechanism of vasorelaxant effects of YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole), a nitric oxide (NO)-independent soluble guanylate cyclase (sGC activator, and DEA/NO (diethylamine/nitric oxide), a NO-nucleophile adduct, on the human RA. RA segments (n = 25) were obtained from coronary artery bypass grafting patients and were divided into 3-4 turn vascular rings. Using the isolated tissue bath technique, the endothelium-independent vasodilatation function was tested in vitro by the addition of cumulative concentrations of YC-1 (10(-10) to 3 x 10(-7) mol/L) and DEA/NO (10(-8) to 3 x 10(-5) mol/L) following vasocontraction by phenylephrine in the presence or absence of 10-5 mol/L ODQ (1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1-one), the selective sGC inhibitor, 10(-7) mol/L iberiotoxin, a blocker of Ca2+- activated K+ channels, or 10(-5) mol/L ODQ plus 10(-7) mol/L iberiotoxin. We also evaluated the effect of YC-1 and DEA/NO on the cGMP levels in vascular rings obtained from human radial artery (n = 6 for each drug). YC-1 (10(-10) to 3 x 10(-7) mol/L) and DEA/NO (10(-8) to 3 x 10(-5) mol/L) caused the concentration-dependent vasorelaxation in RA rings precontracted with phenylephrine (10(-5) mol/L) (n = 20 for each drug). Pre-incubation of RA rings with ODQ, iberiotoxin, or ODQ plus iberiotoxin significantly inhibited the vasorelaxant effect of YC-1, but the inhibitor effect of ODQ plus iberiotoxin was significantly more than that of ODQ and iberiotoxin alone p < 0.05). The vasorelaxant effect of DEA/NO almost completely abolished in the presence of ODQ and iberiotoxin plus ODQ, but did not significantly change in the presence of iberiotoxin alone (P > 0.05). The pEC(50) value of DEA/NO was significantly lower than those for YC-1 (p < 0.01), with no change E. x values in RA rings. In addition, YC- I -stimulated RA rings showed more elevation in cGMP than that of DEA/NO (p < 0.05). These findings indicate that YC-1 is a more potent relaxant than DEA/NO in the human RA. The relaxant effects of YC-1 could be due to the stimulation of the sGC and Ca2+- sensitive K(+)channels, whereas the relaxant effects of DEA/NO could be completely due to the stimulation of the sGC. YC-1 and DEA/NO may be effective as vasodilator for the short-term treatment of perioperative spasm of coronary bypass grafts.en_US
dc.language.isoengen_US
dc.publisherNATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESSen_US
dc.relation.isversionof10.1139/Y07-033en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectYC-1en_US
dc.subjectDEA/NOen_US
dc.subjectcGMPen_US
dc.subjectradial arteryen_US
dc.titleInvestigation of the vasorelaxant effects of 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole (YC-1) and diethylamine/nitric oxide (DEA/NO) on the human radial artery used as coronary bypass graften_US
dc.typearticleen_US
dc.relation.journalCANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGYen_US
dc.contributor.departmentCumhuriyet Univ Sch Med, Dept Cardiovasc Surg, TR-58140 Sivas, Turkey -- Cumhuriyet Univ Sch Med, Dept Pharmacol, TR-58140 Sivas, Turkeyen_US
dc.identifier.volume85en_US
dc.identifier.issue5en_US
dc.identifier.endpage526en_US
dc.identifier.startpage521en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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