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dc.contributor.authorAkkaya, Birnur
dc.contributor.authorUzun, Lokman
dc.contributor.authorCandan, Ferda
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:17:25Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:17:25Z
dc.date.issued2007
dc.identifier.issn0928-4931
dc.identifier.urihttps://dx.doi.org/10.1016/j.msec.2006.04.009
dc.identifier.urihttps://hdl.handle.net/20.500.12418/10720
dc.descriptionWOS: 000243833600031en_US
dc.description.abstractA magnetic metal-chelate adsorbent utilizing N-methacryloyl-(L)-histidine methyl ester (MAH) as a metal-chelating ligand was prepared. MAH was synthesized using methacryloyl chloride and L-histidine methyl ester. Magnetic beads with an average diameter of 50-100 mu m were produced by suspension polymerization of ethylene glycol dimethacrylate (EGDMA) and MAH carried out in a dispersion medium. Specific surface area of the magnetic beads was found to be 80 m(2)/g. Elemental analysis of the magnetic beads for nitrogen was estimated as 70 mu mol MAH/g polymer. Magnetic beads were complexed with the Cu2+ ions directly via MAH for the adsorption of cytochrome c from aqueous solutions. The cytochrome c adsorption on the mag-poly(EGDMA-MAH) beads was 51 mg/g. Cu2+ complexing increased the cytochrome c adsorption significantly. The maximum cytochrome c adsorption capacity of the Cu2+-chelated beads (carrying 68 mu mol Cu2+ per gram of polymer) was found to be 222 mg/g at pH 8.0 in phosphate buffer. Cytochrome c adsorption decreased with increasing temperature. Cytochrome c molecules could be reversibly adsorbed and desorbed ten times with the magnetic adsorbents without noticeable loss in their cytochrome c adsorption capacity. The resulting magnetic chelator beads posses excellent long term storage stability. (c) 2006 Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.isversionof10.1016/j.msec.2006.04.009en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaffinity chromatographyen_US
dc.subjectmetal-chelate affinity beadsen_US
dc.subjecthistidineen_US
dc.subjectcytochrome cen_US
dc.subjectmagnetic polymersen_US
dc.titleN-methacryloyl-(L)-histidine methyl ester carrying porous magnetic beads for metal chelate adsorption of cytochrome cen_US
dc.typearticleen_US
dc.relation.journalMATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMSen_US
dc.contributor.departmentHacettepe Univ, Dept Chem, Div Biochem, Ankara, Turkey -- Cumhuriyet Univ, Dept Chem, Sivas, Turkeyen_US
dc.contributor.authorIDUzun, Lokman -- 0000-0002-3971-7725en_US
dc.identifier.volume27en_US
dc.identifier.issue1en_US
dc.identifier.endpage187en_US
dc.identifier.startpage180en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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