dc.contributor.author | Akkaya, Birnur | |
dc.contributor.author | Uzun, Lokman | |
dc.contributor.author | Candan, Ferda | |
dc.contributor.author | Denizli, Adil | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:17:25Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:17:25Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0928-4931 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.msec.2006.04.009 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/10720 | |
dc.description | WOS: 000243833600031 | en_US |
dc.description.abstract | A magnetic metal-chelate adsorbent utilizing N-methacryloyl-(L)-histidine methyl ester (MAH) as a metal-chelating ligand was prepared. MAH was synthesized using methacryloyl chloride and L-histidine methyl ester. Magnetic beads with an average diameter of 50-100 mu m were produced by suspension polymerization of ethylene glycol dimethacrylate (EGDMA) and MAH carried out in a dispersion medium. Specific surface area of the magnetic beads was found to be 80 m(2)/g. Elemental analysis of the magnetic beads for nitrogen was estimated as 70 mu mol MAH/g polymer. Magnetic beads were complexed with the Cu2+ ions directly via MAH for the adsorption of cytochrome c from aqueous solutions. The cytochrome c adsorption on the mag-poly(EGDMA-MAH) beads was 51 mg/g. Cu2+ complexing increased the cytochrome c adsorption significantly. The maximum cytochrome c adsorption capacity of the Cu2+-chelated beads (carrying 68 mu mol Cu2+ per gram of polymer) was found to be 222 mg/g at pH 8.0 in phosphate buffer. Cytochrome c adsorption decreased with increasing temperature. Cytochrome c molecules could be reversibly adsorbed and desorbed ten times with the magnetic adsorbents without noticeable loss in their cytochrome c adsorption capacity. The resulting magnetic chelator beads posses excellent long term storage stability. (c) 2006 Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.msec.2006.04.009 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | affinity chromatography | en_US |
dc.subject | metal-chelate affinity beads | en_US |
dc.subject | histidine | en_US |
dc.subject | cytochrome c | en_US |
dc.subject | magnetic polymers | en_US |
dc.title | N-methacryloyl-(L)-histidine methyl ester carrying porous magnetic beads for metal chelate adsorption of cytochrome c | en_US |
dc.type | article | en_US |
dc.relation.journal | MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | en_US |
dc.contributor.department | Hacettepe Univ, Dept Chem, Div Biochem, Ankara, Turkey -- Cumhuriyet Univ, Dept Chem, Sivas, Turkey | en_US |
dc.contributor.authorID | Uzun, Lokman -- 0000-0002-3971-7725 | en_US |
dc.identifier.volume | 27 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 187 | en_US |
dc.identifier.startpage | 180 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |