dc.contributor.author | Bagcivan, I | |
dc.contributor.author | Kaya, T | |
dc.contributor.author | Turan, M | |
dc.contributor.author | Goktas, S | |
dc.contributor.author | Demirel, Y | |
dc.contributor.author | Gursoy, S | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:22:30Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:22:30Z | |
dc.date.issued | 2004 | |
dc.identifier.issn | 0008-4212 | |
dc.identifier.issn | 1205-7541 | |
dc.identifier.uri | https://dx.doi.org/10.1139/Y04-073 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/11137 | |
dc.description | WOS: 000226483900001 | en_US |
dc.description | PubMed ID: 15644932 | en_US |
dc.description.abstract | Possible mechanisms for nicotine-induced relaxation were investigated in the isolated sheep's sphincter of Oddi. Sheep's sphincter of Oddi rings were mounted in tissue bath with modified Krebs-Henseleit solution and aerated with 95% oxygen and 5% carbon dioxide. Tension was measured with isometric force transducers, and muscle relaxation was expressed as percent decrease of precontraction induced by carbachol. Nicotine (1 x 10(-5) to 3 x 10(-3) mol/L) produced concentration-dependent relaxation on sphincter of Oddi precontracted by carbachol (10(-6) mol/L). Nicotine-induced relaxation was 72.8 +/- 4.2% of precontraction with carbachol (10(-6) mol/L) (mean pD(2) value, 3.76 +/- 0.05 mol/L). Nicotine-induced relaxation was not affected by N(w)-nitro L-arginine methyl ester (L-NAME) (3 x 10(-5) mol/L), methylene blue (10(-5) mol/L), indomethacin (10(-5) mol/L), hexamethonium (10(-5) mol/L), glibenclamide (10(-5) mol/L), 4-aminopyridine (10(-3) mol/L), tetraethylammonium (3 x 10(-4) mol/L), clotrimazole (10(-6) mol/L), 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) (10(-6) mol/L), and anthracene-9-carboxylate (9-AC) (10(-6) mol/L), but potentiated by bupivacain (10(-5) mol/L). A calcium-antagonizing effect of nicotine was not observed. The results suggest that nicotine-induced relaxation of the sheep's sphincter of Oddi is not mediated by the release of Prostaglandins, nitric oxide (NO), or a related substance; by the activation of potassium channels or chloride channels; or by the stimulation of nicotinic cholinoceptors. Potentiation of the nicotine-induced relaxation by bupivacain indicates that blockade of sodium channels may play a role in this relaxation. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | en_US |
dc.relation.isversionof | 10.1139/Y04-073 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | nicotine | en_US |
dc.subject | sphincter of Oddi | en_US |
dc.subject | relaxation | en_US |
dc.title | Investigation of the mechanism of nicotine-induced relaxation on the sheep sphincter of Oddi | en_US |
dc.type | article | en_US |
dc.relation.journal | CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | en_US |
dc.contributor.department | Cumhuriyet Univ, Sch Med, Dept Pharmacol, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Sch Med, Dept Surg, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Sch Med, Dept Family Med, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Sch Med, Dept Anesthesiol, TR-58140 Sivas, Turkey | en_US |
dc.identifier.volume | 82 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.endpage | 939 | en_US |
dc.identifier.startpage | 935 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |