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dc.contributor.authorCetin, A
dc.contributor.authorKaya, T
dc.contributor.authorDemirkoprulu, N
dc.contributor.authorKaradas, B
dc.contributor.authorDuran, B
dc.contributor.authorCetin, M
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:23:03Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:23:03Z
dc.date.issued2004
dc.identifier.issn1347-8613
dc.identifier.urihttps://dx.doi.org/10.1254/jphs.94.19
dc.identifier.urihttps://hdl.handle.net/20.500.12418/11283
dc.descriptionWOS: 000188469000002en_US
dc.descriptionPubMed ID: 14745113en_US
dc.description.abstractThe aim of this study was to investigate the effect of YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole) on spontaneous contractions and levels of cyclic GMP (cGMP) of myometrial strips isolated from pregnant rats. It is a nitric oxide-independent soluble guanylate cyclase activator. Myometrial strips were obtained from eight pregnant Wistar albino rats and were mounted in organ baths for the recording of isometric tensions. We evaluated the effect of increasing concentrations of YC-1 on spontaneous myometrial contractions and on contractions of myometrial smooth muscle pretreated with methylene blue (10(-5) M), tetraethylammonium chloride (TEA) (3 x 10(-4) M), and glibenclamide (10(-6) M). YC-1 (10(-9)-3 x 10(-5) M) concentration-dependently decreased the amplitude and frequency of spontaneous contractions of myometrial strips. The inhibition of the amplitude and frequency of spontaneous contractions by YC-1 were antagonized with methylene blue (10(-5) M) and TEA (3 x 10(-4) M), but they were not changed by glibenclamide (10(-6) M); however, the antagonistic effect of methylene blue was significantly more than that of TEA (P<0.05). We also evaluated the effect of YC-1 on the levels of cGMP in myometrial strips obtained from pregnant rat uterine horns. YC-1-stimulated myometrial strips showed an excessive elevation in myometrial cGMP that declined slowly during the subsequent washout period. These results show that YC-1 decreases spontaneous contractile activity of myometrial strips isolated from pregnant rat and causes elevation of myometrial cGMP levels in vivo. This effect of YC-1 is significantly reduced by the methylene blue and TEA, suggesting the activation of soluble guanylate cyclase and Ca2+-sensitive K+ channels as the mechanisms of action.en_US
dc.language.isoengen_US
dc.publisherJAPANESE PHARMACOLOGICAL SOCen_US
dc.relation.isversionof10.1254/jphs.94.19en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectYC-1en_US
dc.subjectsoluble guanylate cyclase activatoren_US
dc.subjectmyometrium (rat)en_US
dc.titleYC-1, a nitric oxide-independent activator of soluble guanylate cyclase, inhibits the spontaneous contractions of isolated pregnant rat myometriumen_US
dc.typearticleen_US
dc.relation.journalJOURNAL OF PHARMACOLOGICAL SCIENCESen_US
dc.contributor.departmentCumhuriyet Univ, Sch Med, Dept Obstet & Gynecol, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Sch Med, Dept Pharmacol, TR-58140 Sivas, Turkeyen_US
dc.contributor.authorIDCetin, Ali -- 0000-0002-5767-7894en_US
dc.identifier.volume94en_US
dc.identifier.issue1en_US
dc.identifier.endpage24en_US
dc.identifier.startpage19en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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