dc.contributor.author | Yildiz, E | |
dc.contributor.author | Oztan, A | |
dc.contributor.author | Sar, F | |
dc.contributor.author | Pinarbasi, E | |
dc.contributor.author | Cetin-Atalay, R | |
dc.contributor.author | Akkiz, H | |
dc.contributor.author | Ozturk, M | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:24:05Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:24:05Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0920-8569 | |
dc.identifier.uri | https://dx.doi.org/10.1023/A:1020161818764 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/11491 | |
dc.description | WOS: 000177948300006 | en_US |
dc.description | PubMed ID: 12416679 | en_US |
dc.description.abstract | Based on direct sequencing information from 5'UTR and NS5B regions, we identified subtype 1b as a predominant hepatitis C virus genome in Turkey, which affected more than 91% of 79 patients studied. Next, the full genome sequence of a Turkish 1b isolate was obtained by the cloning of polypeptide-encoding region into 7 overlapping fragments. Turkish 1b isolate, which was named HCV-TR1, comprises 9361 nucleotides, including 306 nucleotides of 5'UTR, a single long open reading frame of 9033 nucleotides, and 22 nucleotides of 3'UTR. When compared to HCV 1b polypeptide sequences available at GenBank, the predicted polypeptide displayed a total of 36 amino acid substitutions, of which 16 was specific for HCV-TR1 isolate. Despite these changes, major structural and functional motifs of HCV proteins were maintained in HCV-TR1. In contrast, HCV-TR1 displayed amino acid substitutions in 6 out of 9 major cytotoxic T-cell epitopes. These data suggest that HCV-TR1 encodes functionally intact viral proteins, but it also encodes altered viral epitopes, which may affect host immune-response. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | KLUWER ACADEMIC PUBL | en_US |
dc.relation.isversionof | 10.1023/A:1020161818764 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | hepatitis C virus | en_US |
dc.subject | subtype 1b | en_US |
dc.subject | HCV-TR1 | en_US |
dc.subject | Turkey | en_US |
dc.subject | viral epitope | en_US |
dc.title | Molecular characterization of a full genome Turkish hepatitis C virus 1b isolate (HCV-TR1): A predominant viral form in Turkey | en_US |
dc.type | article | en_US |
dc.relation.journal | VIRUS GENES | en_US |
dc.contributor.department | Bilkent Univ, Dept Mol Biol & Genet, TR-06533 Ankara, Turkey -- Bilkent Univ, BilGen Genet & Biotechnol, Ctr Res & Dev, TR-06533 Ankara, Turkey -- Cumhuriyet Univ, Fac Med, Dept Med Biol & Genet, Sivas, Turkey -- Cukurova Univ, Fac Med, Dept Hepatol, Adana, Turkey | en_US |
dc.contributor.authorID | Cetin-Atalay, Rengul -- 0000-0003-2408-6606; Cetin-Atalay, Rengul -- 0000-0003-2408-6606; Erdal, Esra -- 0000-0001-7264-0574; Yildiz, Emel -- 0000-0002-3169-491X; Akkiz, Hikmet -- 0000-0001-9745-8875 | en_US |
dc.identifier.volume | 25 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.endpage | 177 | en_US |
dc.identifier.startpage | 169 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |