dc.contributor.author | Durmaz, R | |
dc.contributor.author | Aydin, A | |
dc.contributor.author | Koroglu, M | |
dc.contributor.author | Aker, H | |
dc.contributor.author | Ozercan, IH | |
dc.contributor.author | Atik, E | |
dc.contributor.author | Arici, S | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:26:06Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:26:06Z | |
dc.date.issued | 1998 | |
dc.identifier.issn | 0001-723X | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/11786 | |
dc.description | WOS: 000079531900004 | en_US |
dc.description | PubMed ID: 10358743 | en_US |
dc.description.abstract | In order to determine the positivity rare and genotype of Epstein-Barr virus (EBV) in cases with Hodgkin's disease (HD) in Turkey, 40 tissue specimens from HD patients were analysed. Ten non-lymphoid tissue samples from individuals without any evidence for lymphoma were used as controls. The cases with HD included 33 males and 7 females with a mean age of 28 years. Nodular sclerosis was the most prevalent histological subtype (16/40) followed by mixed cellularity (10/40), lymphocyte predominance (9/40), and lymphocyte depletion (5/40). After histopathological evaluation, deparafinisation and lysis of the specimens, one-stage polymerase chain reaction (PCR) and two-stage (nested) PCR assays were performed with the primers common for both EBV genotypes and the primers specific for EBV types 1 and 2, respectively. EBV DNA was detected in 22 of 40 (55%) cases with HD and in 1 of 10 (10%) control specimens. The distribution of EBV DNA positivity according to the histological subtypes was as follows: 10 of 16 (62.5%) for nodular sclerosis, 3 of 5 (60%) for lymphocyte depletion, 5 of 9 (55.6%) for lymphocyte predominance, and 4 of 10 (40%) for mixed cellularity. Although most of the HD patients were males of 15 - 34 years of age, there were no significant differences between EBV positivities obtained From different sex and age groups. The rates of EBV genotypes were 82% for type 1, 9% for type 2, and 9% for both types, respectively. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SLOVAK ACADEMIC PRESS LTD | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Epstein-Barr virus | en_US |
dc.subject | virus genotype | en_US |
dc.subject | Hodgkin's disease | en_US |
dc.subject | polymerase chain reaction | en_US |
dc.title | Detection and genotyping of Epstein-Barr virus by polymerase chain reaction in tissues obtained from cases with Hodgkin's disease in Turkey | en_US |
dc.type | article | en_US |
dc.relation.journal | ACTA VIROLOGICA | en_US |
dc.contributor.department | Inonu Univ, Fac Med, Dept Clin Microbiol, TR-44069 Malatya, Turkey -- Inonu Univ, Fac Med, Dept Pathol, TR-44069 Malatya, Turkey -- Cumhuriyet Univ, Fac Med, Dept Pathol, Sivas, Turkey -- Firat Univ, Fac Med, Dept Pathol, Elazig, Turkey | en_US |
dc.identifier.volume | 42 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 381 | en_US |
dc.identifier.startpage | 375 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |