dc.contributor.author | Vardar-Unlu, G | |
dc.contributor.author | McSharry, C | |
dc.contributor.author | Douglas, LJ | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:26:26Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:26:26Z | |
dc.date.issued | 1998 | |
dc.identifier.issn | 0928-8244 | |
dc.identifier.issn | 1574-695X | |
dc.identifier.uri | https://dx.doi.org/10.1016/S0928-8244(97)00107-7 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/11827 | |
dc.description | WOS: 000072030900007 | en_US |
dc.description | PubMed ID: 9514576 | en_US |
dc.description.abstract | Lectin-like adhesins of hyphal-form Candida albicans were investigated by conventional fluorescence microscopy, fluorescence microscopy with image analysis, spectrofluorimetry and flow cytometry. Labelling was done with neoglycoprotein probes consisting-of sugars (fucose, mannose, glucose, galactose, lactose) covalently linked to bovine serum albumin (BSA), which itself was labelled with fluorescein. The fucose probe bound to both the yeast and germ-tube portions of hyphal-form cells, not especially at the tip, but in the adjacent region of the germ-tube portion. Probes with the other sugars did not label the hyphal-form cells. Fucose-probe binding to the cells was optimal at pH 5.0 in citrate buffer, and was a time-dependent reaction requiring 30-60 min and reaching saturation concentration at 100 mu g ml(-1). Each hyphal-form cell of C. albicans grown in 199 medium was calculated to have about 2x10(7) fucose probe-binding sites. There appeared to be no requirement for Ca2+ or Mg2+ in binding. Binding of the fucose probe to the hyphal-form cells was higher at 37 degrees C than at 22 degrees C or 4 degrees C, Fluorescence intensity of the fucose-labelled yeast forms was not increased over the hyphal-form cells. A germ-tube-deficient mutant when exposed to hyphal-form growth conditions for 2 h showed much less binding of the fucose probe than the wild-type which produced germ tubes. Confirmation of specificity and the need for a carrier molecule was obtained by showing that Fuc-BSA (without fluorescein) effectively inhibited the binding of the fucose probe, although L-fucose itself was inactive, as was Gal-BSA. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | WILEY | en_US |
dc.relation.isversionof | 10.1016/S0928-8244(97)00107-7 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Candida albicans | en_US |
dc.subject | adhesin | en_US |
dc.subject | germ tube | en_US |
dc.subject | fucose | en_US |
dc.subject | lectin-like | en_US |
dc.title | Fucose-specific adhesins on germ tubes of Candida albicans | en_US |
dc.type | article | en_US |
dc.relation.journal | FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | en_US |
dc.contributor.department | Univ Glasgow, Inst Biomed & Life Sci, Div Infect & Immun, Glasgow G12 8QQ, Lanark, Scotland -- Univ Glasgow, Fac Med, Dept Immunol, Glasgow, Lanark, Scotland | en_US |
dc.identifier.volume | 20 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 67 | en_US |
dc.identifier.startpage | 55 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |