Evaluation of Ki-67 immunostaining in the differential diagnosis of low grade squamous intraepithelial lesion and normal cervix

Abstract

AMAÇ: Ki-67 immun boyaması serviksin skuamöz intraepitelyal lezyonlarda (SIL) ayırıcı tanısında kullanılan tanısal bir testtir. Bu çalışmanın amacı Low-grade SIL ve normal serviksin ayırıcı tanısında Ki-67 indeksini ve Ki-67 pozitif hücre kümelerinin belirlemektir. MATERYAL-METOD: Çalışmada patolojik incelemede 17’si önceden normal serviks, diğer 17’si low-grade SIL tanısı konmuş toplam 34 servikal örnek Ki-67 immunohistokimyasal boyaması yapıldı. SONUÇLAR: Örnekler histo-patolojik olarak ve Ki-67 immun boyaması ile tekrar değerlendirdikten sonra 34 örneğin 21’inde normal serviks ve 13’ünde low-grade SIL tanısı kondu. Ki-67 indeksi low-grade SIL olgularında anlamlı olarak yüksek idi (p<0.05). Ki-67 pozitif hücre kümeleri normal servikste gözlenmedi, 13 low-grade SIL vakasının %77’sinde ise Ki-67 pozitif hücre kümeleri gözlendi (p<0.05). TARTIŞMA: Ki-67 immun boyaması normal serviks ve low-grade SIL olgularının ayırıcı tanısında kolay ve kullanışlı bir teknik olarak düşünülebilir.

OBJECTIVE: Ki-67 immunostaining is a diagnostic adjunctive test used in the differential diagnosis of squamous intraepithelial lesions (SILs) of the cervix. The aim of this study was to determine Ki-67 index and Ki 67-positive cell cluster for distinguishing normal cervix and low-grade squamous intraepithelial lesions (low-grade SILs). MATERIAL-METHOD: Thirty-four cervical specimens, in which 17cases were previously diagnosed as normal cervix and 17 cases were low-grade SILs, included in the study. Ki-67 immunohistochemical staining was performed using avidin biotin complex. RESULTS: After reevaluating the cases by means of histo-pathological and Ki-67 immunostaining features, 21 of 34 cases were diagnosed as normal cervix and 13 were diagnosed as low-grade SILs. Ki-67 index was higher in low-grade SILs (p&lt;0.05). Although Ki-67 positive cell clusters was not observed in normal cervix, 77% of 13 low-grade SILs had positive cell clusters (p&lt;0.05). CONCLUSIONS: Ki-67 immunostaining may be considered as an easy and reproducible technique in differential diagnosis of normal cervix and low-grade SIL.