Karyotype, C- and G-band Patterns and DNA content of Callimenus (=Bradyporus) macrogaster macrogaster

Abstract

Chromosomes and detailed karyotype information (the number, shape, relative length, arm ratio, centromeric index) of Callimenus (=Bradyporus) macrogaster macrogaster Lef. (Orthoptera: Tettigonioidea, Bradyporini) of Turkey belonging to the subfamily Bradyporinae are described. The diploid number of chromosomes was found to be 2n ?= 23, with 2 metacentric pairs, 2 submetacentric pairs, 6 acrocentric pairs. The X chromosome is metacentric. This species has a XX ?/X0 ? sex determining mechanism. The basic karyotype is complemented by a description of C- and G-banding patterns. The C-banding pattern in spermatogonial metaphase was characterized by the presence of paracentromeric C-bands in all chromosomes, and a distal C-band in chromosome 5. The G-banding pattern was complex. The 2C nuclear DNA content was found to be 10.26 ± 0.16 picograms by microspectrophotometry.